Restriction map for
pFN21AE1047
(Circular) (Six-base) MAPSORT of: pFN21AE1047.seq Check: 7268 from: 1 to: 5743
pFN21AE1047 5743 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2979 2979
Size: 5743
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2418 3086 1937
Size: 481 668 4594
Fragments arranged by size:
4594 668 481
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2498 3806 5190 5458 1632
Size: 866 1308 1384 268 1917
Fragments arranged by size:
1917 1384 1308 866 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 2839 4642 5619 1203
Size: 1636 1803 977 1327
Fragments arranged by size:
1803 1636 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2248 2716 2881 3486 5058 2248
Size: 468 165 605 1572 2933
Fragments arranged by size:
2933 1572 605 468 165
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 2669 3052 3278 3430 5539 1493
Size: 1176 383 226 152 2109 1697
Fragments arranged by size:
2109 1697 1176 383 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 2613 3068 1870
Size: 73 192 478 455 4545
Fragments arranged by size:
4545 478 455 192 73
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 4519 618
Size: 325 217 627 1277 1455 1842
Fragments arranged by size:
1842 1455 1277 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2759 3062 1391
Size: 1368 303 4072
Fragments arranged by size:
4072 1368 303
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2348 2439 928
Size: 971 449 91 4232
Fragments arranged by size:
4232 971 449 91
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2696 4354 136
Size: 107 122 71 631 605 1024 1658 1525
Fragments arranged by size:
1658 1525 1024 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2538 5738 2538
Size: 3200 2543
BlpI GC'TnA_GC
Cuts at: 2738 3160 2738
Size: 422 5321
Bme1580I G_kGCm'C
Cuts at: 1790 3804 4960 1790
Size: 2014 1156 2573
Fragments arranged by size:
2573 2014 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3028 3474 4432
Size: 416 53 83 399 24 1611 446 958
Cuts at: 4432 442
Size: 1753
Fragments arranged by size:
1753 1611 958 446 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2113 2423 2430 4403 1239
Size: 874 310 7 1973 2579
Fragments arranged by size:
2579 1973 874 310 7
Bpu10I CC'TnA_GC
Cuts at: 2249 2249
Size: 5743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3868 4733 5031 3868
Size: 865 298 4580
Fragments arranged by size:
4580 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2554 3534 493
Size: 711 1350 980 2702
Fragments arranged by size:
2702 1350 980 711
BsaBI GATnn'nnATC
Cuts at: 2013 2543 5737 2013
Size: 530 3194 2019
Fragments arranged by size:
3194 2019 530
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2436 3550 3932 4567
Size: 53 83 186 936 903 1114 382 635
Cuts at: 4567 275
Size: 1451
Fragments arranged by size:
1451 1114 936 903 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5518
Fragments arranged by size:
5518 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2810 2971 1186
Size: 1624 161 3958
Fragments arranged by size:
3958 1624 161
BseYI C'CCAG_C
Cuts at: 2346 2472 2821 2848 2935 4946 5442 2346
Size: 126 349 27 87 2011 496 2647
Fragments arranged by size:
2647 2011 496 349 126 87 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 2396 2410 1163
Size: 573 456 204 14 4496
Fragments arranged by size:
4496 573 456 204 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2553 2958 3804 3889 4960 2553
Size: 405 846 85 1071 3336
Fragments arranged by size:
3336 1071 846 405 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2553 2759 2958 3062 3804 3889 4960
Size: 399 763 206 199 104 742 85 1071
Cuts at: 4960 1391
Size: 2174
Fragments arranged by size:
2174 1071 763 742 399 206 199 104
85
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2979 3098 844
Size: 2135 119 3489
Fragments arranged by size:
3489 2135 119
BsrBI CCG'CTC
Cuts at: 1394 1823 2224 3630 1394
Size: 429 401 1406 3507
Fragments arranged by size:
3507 1406 429 401
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2901 4387 4574 1700
Size: 110 1091 1486 187 2869
Fragments arranged by size:
2869 1486 1091 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2014 2538 3073 3440 3825 3842 5294 5380
Size: 42 524 535 367 385 17 1452 86
Cuts at: 5380 5392 5738 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 535 524 385 367 346 86
42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2284 2384 2480 2790 513
Size: 552 939 280 100 96 310 3466
Fragments arranged by size:
3466 939 552 310 280 100 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 2198 2749 3259 4053 4073 2198
Size: 551 510 794 20 3868
Fragments arranged by size:
3868 794 551 510 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 2556 3190
Size: 54 1425 50 275 18 489 237 634
Cuts at: 3190 4079 5708 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 634 489 275 237 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 2910 3673 1353
Size: 752 805 763 3423
Fragments arranged by size:
3423 805 763 752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2677 4330 4704 4850 387
Size: 1064 138 1088 1653 374 146 1280
Fragments arranged by size:
1653 1280 1088 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2113 2423 2430 2498 3806 4403 5190
Size: 393 481 310 7 68 1308 597 787
Cuts at: 5190 5458 1239
Size: 268 1524
Fragments arranged by size:
1524 1308 787 597 481 393 310 268
68 7
EcoO109I rG'GnC_Cy
Cuts at: 2596 3030 2596
Size: 434 5309
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FspI TGC'GCA
Cuts at: 2489 4249 2489
Size: 1760 3983
HaeII r_GCGC'y
Cuts at: 1678 2248 2357 2455 4890 1678
Size: 570 109 98 2435 2531
Fragments arranged by size:
2531 2435 570 109 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4384 4416 4458 4490 1373
Size: 32 135 32 2812 32 42 32 2626
Fragments arranged by size:
2812 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2556 4857 5041 1305
Size: 1251 2301 184 2007
Fragments arranged by size:
2301 2007 1251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3921
Fragments arranged by size:
3921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3701 4060 4219 518
Size: 648 2535 359 159 2042
Fragments arranged by size:
2535 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2206 2286 2350 2386 2482 2664 2821 3159
Size: 600 80 64 36 96 182 157 338
Cuts at: 3159 3836 4984 5229 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 600 338 245 182 157
96 80 64 36
NcoI C'CATG_G
Cuts at: 513 1065 2004 2790 513
Size: 552 939 786 3466
Fragments arranged by size:
3466 939 786 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 3101 4646 1132
Size: 97 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2694 2716 1348
Size: 1346 22 4375
Fragments arranged by size:
4375 1346 22
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2855 2881 1108
Size: 1747 26 3970
Fragments arranged by size:
3970 1747 26
PfoI T'CCnGG_A
Cuts at: 2500 2500
Size: 5743
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PmlI CAC'GTG
Cuts at: 2554 2554
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2596 3030 2596
Size: 434 5309
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2205 2361 2460 2712 3095 830
Size: 1375 156 99 252 383 3478
Fragments arranged by size:
3478 1375 383 252 156 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
PvuII CAG'CTG
Cuts at: 2206 2350 2206
Size: 144 5599
SacII CC_GC'GG
Cuts at: 2287 2387 2483 2287
Size: 100 96 5547
Fragments arranged by size:
5547 100 96
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SanDI GG'GwC_CC
Cuts at: 3030 3030
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SexAI A'CCwGG_T
Cuts at: 2775 2775
Size: 5743
SfcI C'TryA_G
Cuts at: 826 1045 2201 2357 2456 2708 3091 4226 4907
Size: 219 1156 156 99 252 383 1135 681
Cuts at: 4907 5098 826
Size: 191 1471
Fragments arranged by size:
1471 1156 1135 681 383 252 219 191
156 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3847 4748 5010 820
Size: 197 926 1904 901 262 1553
Fragments arranged by size:
1904 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 2400 2684 1517
Size: 883 284 4576
Fragments arranged by size:
4576 883 284
StyI C'CwwG_G
Cuts at: 513 1065 2004 2790 2854 3182 513
Size: 552 939 786 64 328 3074
Fragments arranged by size:
3074 939 786 552 328 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3989 5701 96
Size: 274 80 33 51 1443 2012 1712 138
Fragments arranged by size:
2012 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2482 2514 5703 5735 2482
Size: 32 3189 32 2490
Fragments arranged by size:
3189 2490 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3034 1316
Size: 1718 4025
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SapI SfiI SfoI SrfI SwaI XcmI