Restriction map for
pFN21AE1044
(Circular) (Six-base) MAPSORT of: pFN21AE1044.seq Check: 5196 from: 1 to: 6451
pFN21AE1044 6451 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4261 278
Size: 53 83 186 936 2725 2468
Fragments arranged by size:
2725 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3794 1937
Size: 1857 4594
Acc65I G'GTAC_C
Cuts at: 3772 3772
Size: 6451
AclI AA'CG_TT
Cuts at: 1563 4578 4951 1563
Size: 3015 373 3063
Fragments arranged by size:
3063 3015 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2330 2408 2421 3588 4514 5898 6166 1632
Size: 698 78 13 1167 926 1384 268 1917
Fragments arranged by size:
1917 1384 1167 926 698 268 78 13
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6254
AflIII A'CryG_T
Cuts at: 1203 5350 6327 1203
Size: 4147 977 1327
Fragments arranged by size:
4147 1327 977
AgeI A'CCGG_T
Cuts at: 5282 5282
Size: 6451
AhdI GACnn_n'nnGTC
Cuts at: 5180 5180
Size: 6451
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3054 1184
Size: 1870 4581
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 6419
AlwNI CAG_nnn'CTG
Cuts at: 3188 4194 5766 3188
Size: 1006 1572 3873
Fragments arranged by size:
3873 1572 1006
ApaI G_GGCC'C
Cuts at: 3339 3339
Size: 6451
ApaLI G'TGCA_C
Cuts at: 4508 5664 4508
Size: 1156 5295
ApoI r'AATT_y
Cuts at: 1493 2012 2061 2885 3116 3646 3760 3986 4138
Size: 519 49 824 231 530 114 226 152
Cuts at: 4138 6247 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 824 530 519 231 226 152
114 49
AseI AT'TA_AT
Cuts at: 160 1032 5005 160
Size: 872 3973 1606
Fragments arranged by size:
3973 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
AvaI C'yCGr_G
Cuts at: 1870 1943 3651 3776 1870
Size: 73 1708 125 4545
Fragments arranged by size:
4545 1708 125 73
BamHI G'GATC_C
Cuts at: 3781 4148 3781
Size: 367 6084
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 3429 3772 5227 618
Size: 325 217 627 546 1096 343 1455 1842
Fragments arranged by size:
1842 1455 1096 627 546 343 325 217
BanII G_rGCy'C
Cuts at: 1391 2901 3339 3770 1391
Size: 1510 438 431 4072
Fragments arranged by size:
4072 1510 438 431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2396 3285 928
Size: 971 497 889 4094
Fragments arranged by size:
4094 971 889 497
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4642 4676 1903
Size: 34 2705 34 3678
Fragments arranged by size:
3678 2705 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4343 5553 4343
Size: 1210 5241
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6451
BfrBI ATG'CAT
Cuts at: 2459 2459
Size: 6451
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2554 3265 5062
Size: 107 122 71 631 605 882 711 1797
Cuts at: 5062 136
Size: 1525
Fragments arranged by size:
1797 1525 882 711 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2383 6446 2383
Size: 4063 2388
BlpI GC'TnA_GC
Cuts at: 3461 3868 3461
Size: 407 6044
Bme1580I G_kGCm'C
Cuts at: 1790 2255 2338 3339 3534 3585 4512 5668 1790
Size: 465 83 1001 195 51 927 1156 2573
Fragments arranged by size:
2573 1156 1001 927 465 195 83 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6451
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4182 5140 442
Size: 416 53 83 399 24 2765 958 1753
Fragments arranged by size:
2765 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2752 2812 5111 1239
Size: 1513 60 2299 2579
Fragments arranged by size:
2579 2299 1513 60
Bpu10I CC'TnA_GC
Cuts at: 3071 3071
Size: 6451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3222 4576 5441 5739 3222
Size: 1354 865 298 3934
Fragments arranged by size:
3934 1354 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2841 3236 3279 5114 882
Size: 617 1342 395 43 1835 2219
Fragments arranged by size:
2219 1835 1342 617 395 43
BsaAI yAC'GTr
Cuts at: 493 1204 2951 4242 493
Size: 711 1747 1291 2702
Fragments arranged by size:
2702 1747 1291 711
BsaBI GATnn'nnATC
Cuts at: 3409 6445 3409
Size: 3036 3415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4258 4640 5275 275
Size: 53 83 186 936 2725 382 635 1451
Fragments arranged by size:
2725 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4884 5282 5556 5703 6114 4884
Size: 398 274 147 411 5221
Fragments arranged by size:
5221 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2234 2264 3134 3164 3622
Size: 30 165 30 831 30 870 30 458
Cuts at: 3622 3652 1178
Size: 30 3977
Fragments arranged by size:
3977 870 831 458 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2498 2909 3107 3155 3543 3758 3761
Size: 1069 243 411 198 48 388 215 3
Cuts at: 3761 1186
Size: 3876
Fragments arranged by size:
3876 1069 411 388 243 215 198 48
3
BseYI C'CCAG_C
Cuts at: 3181 3231 3356 3437 3722 5654 6150 3181
Size: 50 125 81 285 1932 496 3482
Fragments arranged by size:
3482 1932 496 285 125 81 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2877 1163
Size: 573 1141 4737
Fragments arranged by size:
4737 1141 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3901 4662 5690 1540
Size: 275 186 1900 761 1028 2301
Fragments arranged by size:
2301 1900 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2901 4512 4597 5668 2901
Size: 1611 85 1071 3684
Fragments arranged by size:
3684 1611 1071 85
BsmI GAATG_Cn'
Cuts at: 3962 4055 3962
Size: 93 6358
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2255 2338 2901 3339 3534 3585 3770
Size: 399 465 83 563 438 195 51 185
Cuts at: 3770 4512 4597 5668 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 563 465 438 399 195
185 85 83 51
BspEI T'CCGG_A
Cuts at: 6114 6114
Size: 6451
BspHI T'CATG_A
Cuts at: 4340 6070 4340
Size: 1730 4721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2201 3806 844
Size: 1357 1605 3489
Fragments arranged by size:
3489 1605 1357
BsrBI CCG'CTC
Cuts at: 1394 1823 4338 1394
Size: 429 2515 3507
Fragments arranged by size:
3507 2515 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2374 4946 5120 65
Size: 1183 1126 2572 174 1396
Fragments arranged by size:
2572 1396 1183 1126 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5095 5282 1700
Size: 110 3285 187 2869
Fragments arranged by size:
3285 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6451
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6451
BssSI C'ACGA_G
Cuts at: 4511 5523 4511
Size: 1012 5439
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6451
BstBI TT'CG_AA
Cuts at: 5255 5255
Size: 6451
BstEII G'GTnAC_C
Cuts at: 1184 2188 3299 3655 1184
Size: 1004 1111 356 3980
Fragments arranged by size:
3980 1111 1004 356
BstXI CCAn_nnnn'nTGG
Cuts at: 2822 2995 2822
Size: 173 6278
BstYI r'GATC_y
Cuts at: 1972 2091 2383 3781 4148 4533 4550 6002 6088
Size: 119 292 1398 367 385 17 1452 86
Cuts at: 6088 6100 6446 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1398 385 367 346 292 119
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4960
Fragments arranged by size:
4960 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5712
Fragments arranged by size:
5712 683 56
BtsI GCAGTG_nn'
Cuts at: 3186 3967 4761 4781 3186
Size: 781 794 20 4856
Fragments arranged by size:
4856 794 781 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2683 2718 529
Size: 35 2119 35 4262
Fragments arranged by size:
4262 2119 35 35
DraI TTT'AAA
Cuts at: 3755 3755
Size: 6451
DraIII CAC_nnn'GTG
Cuts at: 3088 3088
Size: 6451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5458 809
Size: 507 4142 1802
Fragments arranged by size:
4142 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3898 4787 6416
Size: 54 1425 50 275 18 2068 889 1629
Cuts at: 6416 8
Size: 43
Fragments arranged by size:
2068 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3898 1537
Size: 275 2086 4090
Fragments arranged by size:
4090 2086 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2213 2703 2986 3132 3729 3735 4381 1353
Size: 860 490 283 146 597 6 646 3423
Fragments arranged by size:
3423 860 646 597 490 283 146 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3508 5038 5412 5558 387
Size: 1064 138 1919 1530 374 146 1280
Fragments arranged by size:
1919 1530 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2899 2899
Size: 6451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2408 2421 2752 2812 3588 4514
Size: 393 698 78 13 331 60 776 926
Cuts at: 4514 5111 5898 6166 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 926 787 776 698 597 393 331
268 78 60 13
EcoO109I rG'GnC_Cy
Cuts at: 2556 3416 2556
Size: 860 5591
EcoRI G'AATT_C
Cuts at: 3116 3646 3760 4138 3116
Size: 530 114 378 5429
Fragments arranged by size:
5429 530 378 114
FspI TGC'GCA
Cuts at: 4957 4957
Size: 6451
HaeII r_GCGC'y
Cuts at: 1678 5598 1678
Size: 3920 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2083 2115 2911 2943 3535
Size: 32 135 32 511 32 796 32 592
Cuts at: 3535 3567 5092 5124 5166 5198 1373
Size: 32 1525 32 42 32 2626
Fragments arranged by size:
2626 1525 796 592 511 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3795 4041 669
Size: 988 281 1857 246 3079
Fragments arranged by size:
3079 1857 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6451
HpaI GTT'AAC
Cuts at: 4041 4041
Size: 6451
KpnI G_GTAC'C
Cuts at: 3776 3776
Size: 6451
MfeI C'AATT_G
Cuts at: 4050 4050
Size: 6451
MluI A'CGCG_T
Cuts at: 6327 6327
Size: 6451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 5565 5749 1305
Size: 888 3372 184 2007
Fragments arranged by size:
3372 2007 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4629
Fragments arranged by size:
4629 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 4409 4768 4927 518
Size: 648 948 2295 359 159 2042
Fragments arranged by size:
2295 2042 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2861 3133 3668 3867 4544 5692 5937 1606
Size: 1255 272 535 199 677 1148 245 2120
Fragments arranged by size:
2120 1255 1148 677 535 272 245 199
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4960
Fragments arranged by size:
4960 939 552
NdeI CA'TA_TG
Cuts at: 387 2461 387
Size: 2074 4377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6451
NotI GC'GGCC_GC
Cuts at: 3898 3898
Size: 6451
NsiI A_TGCA'T
Cuts at: 2461 2461
Size: 6451
NspI r_CATG'y
Cuts at: 1132 1229 3809 5354 1132
Size: 97 2580 1545 2229
Fragments arranged by size:
2580 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3527 3596 1348
Size: 2179 69 4203
Fragments arranged by size:
4203 2179 69
PciI A'CATG_T
Cuts at: 5350 5350
Size: 6451
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6451
PmeI GTTT'AAAC
Cuts at: 3755 3755
Size: 6451
PmlI CAC'GTG
Cuts at: 2951 2951
Size: 6451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 3166 4520 4552 6033 6065 3134
Size: 32 1354 32 1481 32 3520
Fragments arranged by size:
3520 1481 1354 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6451
PsiI TTA'TAA
Cuts at: 4021 4021
Size: 6451
PspOMI G'GGCC_C
Cuts at: 3335 3335
Size: 6451
PstI C_TGCA'G
Cuts at: 830 2176 3667 3803 830
Size: 1346 1491 136 3478
Fragments arranged by size:
3478 1491 1346 136
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6451
PvuII CAG'CTG
Cuts at: 2861 3133 3668 2861
Size: 272 535 5644
Fragments arranged by size:
5644 535 272
SacI G_AGCT'C
Cuts at: 2901 2901
Size: 6451
SalI G'TCGA_C
Cuts at: 1936 3793 1936
Size: 1857 4594
SapI GCTCTTCn'nnn_
Cuts at: 2986 2986
Size: 6451
SbfI CC_TGCA'GG
Cuts at: 3803 3803
Size: 6451
ScaI AGT'ACT
Cuts at: 4699 6411 4699
Size: 1712 4739
SfcI C'TryA_G
Cuts at: 826 1045 2172 3663 3799 4934 5615 5806 826
Size: 219 1127 1491 136 1135 681 191 1471
Fragments arranged by size:
1491 1471 1135 1127 681 219 191 136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6451
SmaI CCC'GGG
Cuts at: 1872 3653 3778 1872
Size: 1781 125 4545
Fragments arranged by size:
4545 1781 125
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3237 4555 5456 5718 820
Size: 197 926 1294 1318 901 262 1553
Fragments arranged by size:
1553 1318 1294 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4242 493
Size: 3749 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6451
SphI G_CATG'C
Cuts at: 1132 3809 1132
Size: 2677 3774
SspI AAT'ATT
Cuts at: 5 52 4375 5
Size: 47 4323 2081
Fragments arranged by size:
4323 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6451
StyI C'CwwG_G
Cuts at: 513 1065 2004 3890 513
Size: 552 939 1886 3074
Fragments arranged by size:
3074 1886 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2551 3417 4478 4495 4648 4833 5278
Size: 1255 971 866 1061 17 153 185 445
Cuts at: 5278 325
Size: 1498
Fragments arranged by size:
1498 1255 1061 971 866 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4697 6409 96
Size: 274 80 33 51 1443 2720 1712 138
Fragments arranged by size:
2720 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2234 2266 3622 3654 6411 6443 2234
Size: 32 1356 32 2757 32 2242
Fragments arranged by size:
2757 2242 1356 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6451
XbaI T'CTAG_A
Cuts at: 2017 3787 2017
Size: 1770 4681
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2152 2152
Size: 6451
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6451
XmaI C'CCGG_G
Cuts at: 1870 3651 3776 1870
Size: 1781 125 4545
Fragments arranged by size:
4545 1781 125
XmnI GAAnn'nnTTC
Cuts at: 2139 4580 2139
Size: 2441 4010
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4259 276
Size: 53 83 186 936 2725 2468
Fragments arranged by size:
2725 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI