Restriction map for
pFN21AE1042
(Circular) (Six-base) MAPSORT of: pFN21AE1042.seq Check: 7309 from: 1 to: 6208
pFN21AE1042 6208 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3328 4271 5655 5923 1632
Size: 1696 943 1384 268 1917
Fragments arranged by size:
1917 1696 1384 943 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 5107 6084 1203
Size: 3904 977 1327
Fragments arranged by size:
3904 1327 977
AgeI A'CCGG_T
Cuts at: 2157 5039 2157
Size: 2882 3326
AhdI GACnn_n'nnGTC
Cuts at: 2409 4937 2409
Size: 2528 3680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2530 3424 1184
Size: 1346 894 3968
Fragments arranged by size:
3968 1346 894
AlwNI CAG_nnn'CTG
Cuts at: 2722 2974 3951 5523 2722
Size: 252 977 1572 3407
Fragments arranged by size:
3407 1572 977 252
ApaI G_GGCC'C
Cuts at: 3254 3254
Size: 6208
ApaLI G'TGCA_C
Cuts at: 4265 5421 4265
Size: 1156 5052
ApoI r'AATT_y
Cuts at: 1493 3448 3517 3743 3895 6004 1493
Size: 1955 69 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 226 152 69
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2598 2675 3533 1870
Size: 73 655 77 858 4545
Fragments arranged by size:
4545 858 655 77 73
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2506 3203 3529 4984 618
Size: 325 217 627 719 697 326 1455 1842
Fragments arranged by size:
1842 1455 719 697 627 326 325 217
BanII G_rGCy'C
Cuts at: 1391 2753 2808 3254 3507 3527 1391
Size: 1362 55 446 253 20 4072
Fragments arranged by size:
4072 1362 446 253 55 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2843 3055 928
Size: 971 944 212 4081
Fragments arranged by size:
4081 971 944 212
BbvCI CC'TCA_GC
Cuts at: 2189 2189
Size: 6208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2631 2665 4399 4433 1903
Size: 34 694 34 1734 34 3678
Fragments arranged by size:
3678 1734 694 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4100 5310 4100
Size: 1210 4998
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2382 4819 136
Size: 107 122 71 631 605 710 2437 1525
Fragments arranged by size:
2437 1525 710 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3075 6203 3075
Size: 3128 3080
BlpI GC'TnA_GC
Cuts at: 3625 3625
Size: 6208
Bme1580I G_kGCm'C
Cuts at: 1790 2474 2509 3254 4269 5425 1790
Size: 684 35 745 1015 1156 2573
Fragments arranged by size:
2573 1156 1015 745 684 35
BmgBI CAC'GTC
Cuts at: 1318 2527 2620 3355 1318
Size: 1209 93 735 4171
Fragments arranged by size:
4171 1209 735 93
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2322 3939 4897
Size: 416 53 83 399 24 905 1617 958
Cuts at: 4897 442
Size: 1753
Fragments arranged by size:
1753 1617 958 905 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2019 1056
Size: 963 5245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2480 2780 2849 2996 4868 1239
Size: 821 420 300 69 147 1872 2579
Fragments arranged by size:
2579 1872 821 420 300 147 69
Bpu10I CC'TnA_GC
Cuts at: 2189 2486 2189
Size: 297 5911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2978 4333 5198 5496 2978
Size: 1355 865 298 3690
Fragments arranged by size:
3690 1355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2775 4871 882
Size: 617 1276 2096 2219
Fragments arranged by size:
2219 2096 1276 617
BsaAI yAC'GTr
Cuts at: 493 1204 3331 3457 3999 493
Size: 711 2127 126 542 2702
Fragments arranged by size:
2702 2127 711 542 126
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3261 3339 4015 4397
Size: 53 83 186 936 1728 78 676 382
Cuts at: 4397 5032 275
Size: 635 1451
Fragments arranged by size:
1728 1451 936 676 635 382 186 83
78 53
BsaWI w'CCGG_w
Cuts at: 2157 4641 5039 5313 5460 5871 2157
Size: 2484 398 274 147 411 2494
Fragments arranged by size:
2494 2484 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 2453 2483 2511
Size: 30 165 30 687 30 333 30 28
Cuts at: 2511 2541 1178
Size: 30 4845
Fragments arranged by size:
4845 687 333 165 30 30 30 30
30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2995 1186
Size: 1809 4399
BseYI C'CCAG_C
Cuts at: 3139 5411 5907 3139
Size: 2272 496 3440
Fragments arranged by size:
3440 2272 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2735 2987 1163
Size: 573 999 252 4384
Fragments arranged by size:
4384 999 573 252
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2319 3658 4419 5447 1540
Size: 275 186 318 1339 761 1028 2301
Fragments arranged by size:
2301 1339 1028 761 318 275 186
BsiHKAI G_wGCw'C
Cuts at: 2108 2466 2709 2733 2753 2808 3330 4269 4354
Size: 358 243 24 20 55 522 939 85
Cuts at: 4354 5425 2108
Size: 1071 2891
Fragments arranged by size:
2891 1071 939 522 358 243 85 55
24 20
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
BsmBI CGTCTCn'nnnn_
Cuts at: 2672 3433 2672
Size: 761 5447
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2466 2474 2509 2709 2733 2753
Size: 399 318 358 8 35 200 24 20
Cuts at: 2753 2808 3254 3330 3507 3527 4269 4354 5425
Size: 55 446 76 177 20 742 85 1071
Cuts at: 5425 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 446 399 358 318 200
177 85 76 55 35 24 20 20
8
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 4097 5827 4097
Size: 1730 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3147 3563 844
Size: 2303 416 3489
Fragments arranged by size:
3489 2303 416
BsrBI CCG'CTC
Cuts at: 1394 1823 2395 4095 1394
Size: 429 572 1700 3507
Fragments arranged by size:
3507 1700 572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2497 4703 4877 65
Size: 1183 1249 2206 174 1396
Fragments arranged by size:
2206 1396 1249 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2157 2314 2493 2916 2925 4852 5039
Size: 110 347 157 179 423 9 1927 187
Cuts at: 5039 1700
Size: 2869
Fragments arranged by size:
2869 1927 423 347 187 179 157 110
9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6208
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 2225 2465 2540 2708 4268 5280 2225
Size: 240 75 168 1560 1012 3153
Fragments arranged by size:
3153 1560 1012 240 168 75
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2713 2722 794
Size: 1919 9 4280
Fragments arranged by size:
4280 1919 9
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 3292 1184
Size: 2108 4100
BstYI r'GATC_y
Cuts at: 1972 2592 3075 3538 3905 4290 4307 5759 5845
Size: 620 483 463 367 385 17 1452 86
Cuts at: 5845 5857 6203 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 620 483 463 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4717
Fragments arranged by size:
4717 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3020 3382 487
Size: 683 56 1794 362 3313
Fragments arranged by size:
3313 1794 683 362 56
BtsI GCAGTG_nn'
Cuts at: 2153 2723 3724 4518 4538 2153
Size: 570 1001 794 20 3823
Fragments arranged by size:
3823 1001 794 570 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2611 2646 529
Size: 35 2047 35 4091
Fragments arranged by size:
4091 2047 35 35
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DraIII CAC_nnn'GTG
Cuts at: 2713 2713
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5215 809
Size: 507 3899 1802
Fragments arranged by size:
3899 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2152 2178 2316
Size: 54 1425 50 275 18 322 26 138
Cuts at: 2316 2736 2841 2892 3115 3655 4544 6173 8
Size: 420 105 51 223 540 889 1629 43
Fragments arranged by size:
1629 1425 889 540 420 322 275 223
138 105 54 51 50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 2316 3655 1537
Size: 275 504 1339 4090
Fragments arranged by size:
4090 1339 504 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3236 3422 4138 1353
Size: 1883 186 716 3423
Fragments arranged by size:
3423 1883 716 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2098 2423 4795 5169 5315 387
Size: 1064 138 509 325 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 509 374 325 146 138
EcoICRI GAG'CTC
Cuts at: 2751 2806 2751
Size: 55 6153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2480 2780 2849 2996 3328 4271
Size: 393 428 420 300 69 147 332 943
Cuts at: 4271 4868 5655 5923 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 943 787 597 428 420 393 332
300 268 147 69
EcoO109I rG'GnC_Cy
Cuts at: 2133 2352 2998 3250 2133
Size: 219 646 252 5091
Fragments arranged by size:
5091 646 252 219
EcoRI G'AATT_C
Cuts at: 3448 3517 3895 3448
Size: 69 378 5761
Fragments arranged by size:
5761 378 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2578 2791 3313 2578
Size: 213 522 5473
Fragments arranged by size:
5473 522 213
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 2455 3100 5355 1678
Size: 777 645 2255 2531
Fragments arranged by size:
2531 2255 777 645
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2395 2427 4849 4881 4923
Size: 32 135 32 823 32 2422 32 42
Cuts at: 4923 4955 1373
Size: 32 2626
Fragments arranged by size:
2626 2422 823 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3385 3552 3798 669
Size: 988 281 1447 167 246 3079
Fragments arranged by size:
3079 1447 988 281 246 167
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5322 5506 1305
Size: 4017 184 2007
Fragments arranged by size:
4017 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2154 2180 2738 2894 10
Size: 54 1425 343 322 26 558 156 3324
Fragments arranged by size:
3324 1425 558 343 322 156 54 26
MslI CAynn'nnrTG
Cuts at: 518 1166 4166 4525 4684 518
Size: 648 3000 359 159 2042
Fragments arranged by size:
3000 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2388 2974 3624 4301 5449 5694 1606
Size: 782 586 650 677 1148 245 2120
Fragments arranged by size:
2120 1148 782 677 650 586 245
NaeI GCC'GGC
Cuts at: 2316 2495 2918 2316
Size: 179 423 5606
Fragments arranged by size:
5606 423 179
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4717
Fragments arranged by size:
4717 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NgoMIV G'CCGG_C
Cuts at: 2314 2493 2916 2314
Size: 179 423 5606
Fragments arranged by size:
5606 423 179
NheI G'CTAG_C
Cuts at: 1052 2015 1052
Size: 963 5245
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 3566 5111 1132
Size: 97 2337 1545 2229
Fragments arranged by size:
2337 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6208
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6208
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PmlI CAC'GTG
Cuts at: 3331 3331
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2352 2998 2352
Size: 646 5562
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PspOMI G'GGCC_C
Cuts at: 3250 3250
Size: 6208
PstI C_TGCA'G
Cuts at: 830 2014 2117 2814 2915 3560 830
Size: 1184 103 697 101 645 3478
Fragments arranged by size:
3478 1184 697 645 103 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2974 2974
Size: 6208
SacI G_AGCT'C
Cuts at: 2753 2808 2753
Size: 55 6153
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SanDI GG'GwC_CC
Cuts at: 2352 2352
Size: 6208
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SfcI C'TryA_G
Cuts at: 826 1045 2010 2048 2113 2810 2911 3556 4691
Size: 219 965 38 65 697 101 645 1135
Cuts at: 4691 5372 5563 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 965 697 681 645 219 191
101 65 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 2600 3535 1872
Size: 728 935 4545
Fragments arranged by size:
4545 935 728
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2675 2993 4312 5213 5475 820
Size: 197 926 732 318 1319 901 262 1553
Fragments arranged by size:
1553 1319 926 901 732 318 262 197
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 2427 2909 1517
Size: 910 482 4816
Fragments arranged by size:
4816 910 482
StyI C'CwwG_G
Cuts at: 513 1065 2004 2437 3647 513
Size: 552 939 433 1210 3074
Fragments arranged by size:
3074 1210 939 552 433
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 4235 4252 4405 4590 5035 325
Size: 1255 840 1815 17 153 185 445 1498
Fragments arranged by size:
1815 1498 1255 840 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4454 6166 96
Size: 274 80 33 51 1443 2477 1712 138
Fragments arranged by size:
2477 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2453 2485 2511 2543 6168 6200 2453
Size: 32 26 32 3625 32 2461
Fragments arranged by size:
3625 2461 32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 1316 2618 1316
Size: 1302 4906
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2071 2147 2071
Size: 76 6132
XhoI C'TCGA_G
Cuts at: 1943 2675 1943
Size: 732 5476
XmaI C'CCGG_G
Cuts at: 1870 2598 3533 1870
Size: 728 935 4545
Fragments arranged by size:
4545 935 728
XmnI GAAnn'nnTTC
Cuts at: 4337 4337
Size: 6208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PspXI
PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI