Restriction map for
pFN21AE1039
(Circular) (Six-base) MAPSORT of: pFN21AE1039.seq Check: 666 from: 1 to: 6190
pFN21AE1039 6190 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4000 278
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3533 1937
Size: 1596 4594
Acc65I G'GTAC_C
Cuts at: 3511 3511
Size: 6190
AclI AA'CG_TT
Cuts at: 1563 4317 4690 1563
Size: 2754 373 3063
Fragments arranged by size:
3063 2754 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4253 5637 5905 1632
Size: 2621 1384 268 1917
Fragments arranged by size:
2621 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5993
AflIII A'CryG_T
Cuts at: 1203 2519 2853 5089 6066 1203
Size: 1316 334 2236 977 1327
Fragments arranged by size:
2236 1327 1316 977 334
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 4919 4919
Size: 6190
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6190
AlwNI CAG_nnn'CTG
Cuts at: 2897 3061 3933 5505 2897
Size: 164 872 1572 3582
Fragments arranged by size:
3582 1572 872 164
ApaLI G'TGCA_C
Cuts at: 4247 5403 4247
Size: 1156 5034
ApoI r'AATT_y
Cuts at: 1493 2082 2159 2260 2471 2843 3050 3499 3725
Size: 589 77 101 211 372 207 449 226
Cuts at: 3725 3877 5986 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 589 449 372 226 211 207
152 101 77
AseI AT'TA_AT
Cuts at: 160 1032 2652 4744 160
Size: 872 1620 2092 1606
Fragments arranged by size:
2092 1620 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 3515 1870
Size: 73 1572 4545
Fragments arranged by size:
4545 1572 73
BamHI G'GATC_C
Cuts at: 3520 3887 3520
Size: 367 5823
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2835 3511 4966 618
Size: 325 217 627 1048 676 1455 1842
Fragments arranged by size:
1842 1455 1048 676 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2498 3509 1391
Size: 1107 1011 4072
Fragments arranged by size:
4072 1107 1011
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2060 2936 928
Size: 971 161 876 4182
Fragments arranged by size:
4182 971 876 161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4381 4415 1903
Size: 34 2444 34 3678
Fragments arranged by size:
3678 2444 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3077 4082 5292 3077
Size: 1005 1210 3975
Fragments arranged by size:
3975 1210 1005
BclI T'GATC_A
Cuts at: 1548 2415 1548
Size: 867 5323
BfrBI ATG'CAT
Cuts at: 3168 3168
Size: 6190
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4801 136
Size: 107 122 71 631 605 3129 1525
Fragments arranged by size:
3129 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3349 6185 3349
Size: 2836 3354
BlpI GC'TnA_GC
Cuts at: 2559 3607 2559
Size: 1048 5142
Bme1580I G_kGCm'C
Cuts at: 1790 4251 5407 1790
Size: 2461 1156 2573
Fragments arranged by size:
2573 2461 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3921 4879 442
Size: 416 53 83 399 24 2504 958 1753
Fragments arranged by size:
2504 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 6190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2353 2526 4850 1239
Size: 1114 173 2324 2579
Fragments arranged by size:
2579 2324 1114 173
Bpu10I CC'TnA_GC
Cuts at: 2568 2568
Size: 6190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4315 5180 5478 4315
Size: 865 298 5027
Fragments arranged by size:
5027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4853 882
Size: 617 3354 2219
Fragments arranged by size:
3354 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3981 493
Size: 711 2777 2702
Fragments arranged by size:
2777 2702 711
BsaBI GATnn'nnATC
Cuts at: 6184 6184
Size: 6190
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3997 4379 5014 275
Size: 53 83 186 936 2464 382 635 1451
Fragments arranged by size:
2464 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4623 5021 5295 5442 5853 4623
Size: 398 274 147 411 4960
Fragments arranged by size:
4960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5965
Fragments arranged by size:
5965 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6190
BseYI C'CCAG_C
Cuts at: 5393 5889 5393
Size: 496 5694
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5617
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3640 4401 5429 1540
Size: 275 186 1639 761 1028 2301
Fragments arranged by size:
2301 1639 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4251 4336 5407 4251
Size: 85 1071 5034
Fragments arranged by size:
5034 1071 85
BsmI GAATG_Cn'
Cuts at: 3701 3794 3701
Size: 93 6097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2498 3509 4251 4336 5407 1391
Size: 399 708 1011 742 85 1071 2174
Fragments arranged by size:
2174 1071 1011 742 708 399 85
BspEI T'CCGG_A
Cuts at: 5853 5853
Size: 6190
BspHI T'CATG_A
Cuts at: 2049 4079 5809 2049
Size: 2030 1730 2430
Fragments arranged by size:
2430 2030 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2385 3545 844
Size: 1541 1160 3489
Fragments arranged by size:
3489 1541 1160
BsrBI CCG'CTC
Cuts at: 1394 1823 4077 1394
Size: 429 2254 3507
Fragments arranged by size:
3507 2254 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2103 2115 4685 4859 65
Size: 1183 855 12 2570 174 1396
Fragments arranged by size:
2570 1396 1183 855 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 4834 5021 1700
Size: 110 3024 187 2869
Fragments arranged by size:
3024 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2848 2856 96
Size: 2752 8 3430
Fragments arranged by size:
3430 2752 8
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6190
BssSI C'ACGA_G
Cuts at: 4250 5262 4250
Size: 1012 5178
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6190
BstBI TT'CG_AA
Cuts at: 4994 4994
Size: 6190
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 2215 2545 3349 3520 3887 4272 4289 5741
Size: 243 330 804 171 367 385 17 1452
Cuts at: 5741 5827 5839 6185 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 804 385 367 346 330 243
171 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4699
Fragments arranged by size:
4699 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2886 487
Size: 683 56 1660 3791
Fragments arranged by size:
3791 1660 683 56
BtsI GCAGTG_nn'
Cuts at: 3706 4500 4520 3706
Size: 794 20 5376
Fragments arranged by size:
5376 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6155
DraI TTT'AAA
Cuts at: 2382 2772 3494 2382
Size: 390 722 5078
Fragments arranged by size:
5078 722 390
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 3637 4526
Size: 54 1425 50 275 18 186 1621 889
Cuts at: 4526 6155 8
Size: 1629 43
Fragments arranged by size:
1629 1621 1425 889 275 186 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3637 1537
Size: 275 1825 4090
Fragments arranged by size:
4090 1825 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2332 2526 2679 3252 3357 4120 1353
Size: 979 194 153 573 105 763 3423
Fragments arranged by size:
3423 979 763 573 194 153 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4777 5151 5297 387
Size: 1064 138 3188 374 146 1280
Fragments arranged by size:
3188 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2353 2526 4253 4850 5637 5905 1239
Size: 393 721 173 1727 597 787 268 1524
Fragments arranged by size:
1727 1524 787 721 597 393 268 173
EcoRI G'AATT_C
Cuts at: 3499 3877 3499
Size: 378 5812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3311 3311
Size: 6190
FspI TGC'GCA
Cuts at: 4696 4696
Size: 6190
HaeII r_GCGC'y
Cuts at: 1678 5337 1678
Size: 3659 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2104 2136 4831 4863 4905
Size: 32 135 32 532 32 2695 32 42
Cuts at: 4905 4937 1373
Size: 32 2626
Fragments arranged by size:
2695 2626 532 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2632 3534 3780 669
Size: 988 281 694 902 246 3079
Fragments arranged by size:
3079 988 902 694 281 246
HindIII A'AGCT_T
Cuts at: 748 2254 748
Size: 1506 4684
HpaI GTT'AAC
Cuts at: 2632 3780 2632
Size: 1148 5042
KpnI G_GTAC'C
Cuts at: 3515 3515
Size: 6190
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 6190
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2210 5304 5488 1305
Size: 905 3094 184 2007
Fragments arranged by size:
3094 2007 905 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4368
Fragments arranged by size:
4368 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 3045 4148 4507 4666 518
Size: 648 942 937 1103 359 159 2042
Fragments arranged by size:
2042 1103 942 937 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2670 2731 2894 3446 3606 4283 5431 5676
Size: 1064 61 163 552 160 677 1148 245
Cuts at: 5676 1606
Size: 2120
Fragments arranged by size:
2120 1148 1064 677 552 245 163 160
61
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4699
Fragments arranged by size:
4699 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6190
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 3637 3637
Size: 6190
NsiI A_TGCA'T
Cuts at: 3170 3170
Size: 6190
NspI r_CATG'y
Cuts at: 1132 1229 2857 3548 5093 1132
Size: 97 1628 691 1545 2229
Fragments arranged by size:
2229 1628 1545 691 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6190
PciI A'CATG_T
Cuts at: 2853 5089 2853
Size: 2236 3954
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6190
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 5772 5804 4259
Size: 32 1481 32 4645
Fragments arranged by size:
4645 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6190
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PstI C_TGCA'G
Cuts at: 830 2286 3542 830
Size: 1456 1256 3478
Fragments arranged by size:
3478 1456 1256
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
PvuII CAG'CTG
Cuts at: 2670 2894 2670
Size: 224 5966
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 4438 6150 4438
Size: 1712 4478
SfcI C'TryA_G
Cuts at: 826 1045 2282 2361 2526 3144 3538 4673 5354
Size: 219 1237 79 165 618 394 1135 681
Cuts at: 5354 5545 826
Size: 191 1471
Fragments arranged by size:
1471 1237 1135 681 618 394 219 191
165 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 3517 1872
Size: 1645 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4294 5195 5457 820
Size: 197 926 2351 901 262 1553
Fragments arranged by size:
2351 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 3548 1132
Size: 2416 3774
SspI AAT'ATT
Cuts at: 5 52 4114 5
Size: 47 4062 2081
Fragments arranged by size:
4062 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6190
StyI C'CwwG_G
Cuts at: 513 1065 2004 3629 513
Size: 552 939 1625 3074
Fragments arranged by size:
3074 1625 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4217 4234 4387 4572 5017 325
Size: 1255 2637 17 153 185 445 1498
Fragments arranged by size:
2637 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2848 2856 4436
Size: 274 80 33 51 1443 871 8 1580
Cuts at: 4436 6148 96
Size: 1712 138
Fragments arranged by size:
1712 1580 1443 871 274 138 80 51
33 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6150 6182 6150
Size: 32 6158
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6190
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6190
XmaI C'CCGG_G
Cuts at: 1870 3515 1870
Size: 1645 4545
XmnI GAAnn'nnTTC
Cuts at: 2904 4319 2904
Size: 1415 4775
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3998 276
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI