Restriction map for
pFN21AE1036
(Circular) (Six-base) MAPSORT of: pFN21AE1036.seq Check: 4673 from: 1 to: 6379
pFN21AE1036 6379 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2318 2318
Size: 6379
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2478 3722 1937
Size: 541 1244 4594
Fragments arranged by size:
4594 1244 541
Acc65I G'GTAC_C
Cuts at: 3700 3700
Size: 6379
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2181 2406 3377 4442 5826 6094 1632
Size: 549 225 971 1065 1384 268 1917
Fragments arranged by size:
1917 1384 1065 971 549 268 225
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6182
AflIII A'CryG_T
Cuts at: 1203 5278 6255 1203
Size: 4075 977 1327
Fragments arranged by size:
4075 1327 977
AgeI A'CCGG_T
Cuts at: 5210 5210
Size: 6379
AhdI GACnn_n'nnGTC
Cuts at: 3328 5108 3328
Size: 1780 4599
AleI CACnn'nnGTG
Cuts at: 1166 2963 1166
Size: 1797 4582
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2981 1184
Size: 1797 4582
AlwNI CAG_nnn'CTG
Cuts at: 2114 2133 2678 2845 4122 5694 2114
Size: 19 545 167 1277 1572 2799
Fragments arranged by size:
2799 1572 1277 545 167 19
ApaI G_GGCC'C
Cuts at: 2080 2528 3531 2080
Size: 448 1003 4928
Fragments arranged by size:
4928 1003 448
ApaLI G'TGCA_C
Cuts at: 4436 5592 4436
Size: 1156 5223
ApoI r'AATT_y
Cuts at: 1493 3688 3914 4066 6175 1493
Size: 2195 226 152 2109 1697
Fragments arranged by size:
2195 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4933 160
Size: 872 3901 1606
Fragments arranged by size:
3901 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 2517 3381 3704 1870
Size: 73 574 864 323 4545
Fragments arranged by size:
4545 864 574 323 73
AvrII C'CTAG_G
Cuts at: 2029 2650 2029
Size: 621 5758
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3247 3280 3247
Size: 33 6346
BamHI G'GATC_C
Cuts at: 3709 4076 3709
Size: 367 6012
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2284 2655 2754 2965 3154
Size: 325 217 627 497 371 99 211 189
Cuts at: 3154 3672 3700 5155 618
Size: 518 28 1455 1842
Fragments arranged by size:
1842 1455 627 518 497 371 325 217
211 189 99 28
BanII G_rGCy'C
Cuts at: 1391 2080 2528 2565 2832 3531 3698 1391
Size: 689 448 37 267 699 167 4072
Fragments arranged by size:
4072 699 689 448 267 167 37
BbeI G_GCGC'C
Cuts at: 2659 2758 2659
Size: 99 6280
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2412 3395 928
Size: 971 513 983 3912
Fragments arranged by size:
3912 983 971 513
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4570 4604 1903
Size: 34 2633 34 3678
Fragments arranged by size:
3678 2633 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2131 2679 3387 4271 5481 2131
Size: 548 708 884 1210 3029
Fragments arranged by size:
3029 1210 884 708 548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2346 3092 4990
Size: 107 122 71 631 605 674 746 1898
Cuts at: 4990 136
Size: 1525
Fragments arranged by size:
1898 1525 746 674 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6374 6374
Size: 6379
BlpI GC'TnA_GC
Cuts at: 2018 2826 3796 2018
Size: 808 970 4601
Fragments arranged by size:
4601 970 808
Bme1580I G_kGCm'C
Cuts at: 1790 2080 2161 2528 3159 3531 4440 5596 1790
Size: 290 81 367 631 372 909 1156 2573
Fragments arranged by size:
2573 1156 909 631 372 367 290 81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2365 2497 4110
Size: 416 53 83 399 24 948 132 1613
Cuts at: 4110 5068 442
Size: 958 1753
Fragments arranged by size:
1753 1613 958 948 416 399 132 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6379
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 3451 3469 2083
Size: 1368 18 4993
Fragments arranged by size:
4993 1368 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 3208 3464 3562 5039 1239
Size: 944 1025 256 98 1477 2579
Fragments arranged by size:
2579 1477 1025 944 256 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2287 4504 5369 5667 2287
Size: 2217 865 298 2999
Fragments arranged by size:
2999 2217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5042 882
Size: 617 3543 2219
Fragments arranged by size:
3543 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2142 3090 4170 493
Size: 711 938 948 1080 2702
Fragments arranged by size:
2702 1080 948 938 711
BsaBI GATnn'nnATC
Cuts at: 6373 6373
Size: 6379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2099 2656 2755 4186
Size: 53 83 186 936 566 557 99 1431
Cuts at: 4186 4568 5203 275
Size: 382 635 1451
Fragments arranged by size:
1451 1431 936 635 566 557 382 186
99 83 53
BsaWI w'CCGG_w
Cuts at: 2463 3279 4812 5210 5484 5631 6042 2463
Size: 816 1533 398 274 147 411 2800
Fragments arranged by size:
2800 1533 816 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3233 3263 1178
Size: 30 165 30 1830 30 4294
Fragments arranged by size:
4294 1830 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2215 2582 3410 3424 1186
Size: 1029 367 828 14 4141
Fragments arranged by size:
4141 1029 828 367 14
BseYI C'CCAG_C
Cuts at: 2303 2498 2870 3646 5582 6078 2303
Size: 195 372 776 1936 496 2604
Fragments arranged by size:
2604 1936 776 496 372 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 3271 3585 3596 3619 1163
Size: 573 816 719 314 11 23 3923
Fragments arranged by size:
3923 816 719 573 314 23 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3829 4590 5618 1540
Size: 275 186 1828 761 1028 2301
Fragments arranged by size:
2301 1828 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4440 4525 5596 4440
Size: 85 1071 5223
Fragments arranged by size:
5223 1071 85
BsmI GAATG_Cn'
Cuts at: 2872 3890 3983 2872
Size: 1018 93 5268
Fragments arranged by size:
5268 1018 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2090 2899 2090
Size: 809 5570
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2161 2528 2565 2832 3159 3531
Size: 399 290 81 367 37 267 327 372
Cuts at: 3531 3698 4440 4525 5596 1391
Size: 167 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 367 327 290
267 167 85 81 37
BspEI T'CCGG_A
Cuts at: 2463 3279 6042 2463
Size: 816 2763 2800
Fragments arranged by size:
2800 2763 816
BspHI T'CATG_A
Cuts at: 4268 5998 4268
Size: 1730 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2318 2694 3734 844
Size: 1474 376 1040 3489
Fragments arranged by size:
3489 1474 1040 376
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 4266 1394
Size: 429 1684 759 3507
Fragments arranged by size:
3507 1684 759 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2309 2791 3349 4874 5048 65
Size: 1183 1061 482 558 1525 174 1396
Fragments arranged by size:
1525 1396 1183 1061 558 482 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2376 2811 5023 5210 1700
Size: 110 566 435 2212 187 2869
Fragments arranged by size:
2869 2212 566 435 187 110
BsrGI T'GTAC_A
Cuts at: 96 2250 96
Size: 2154 4225
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 4439 5451 4439
Size: 1012 5367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2114 794
Size: 1320 5059
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 3199 1184
Size: 2015 4364
BstXI CCAn_nnnn'nTGG
Cuts at: 2635 2806 2905 3001 3130 3186 2635
Size: 171 99 96 129 56 5828
Fragments arranged by size:
5828 171 129 99 96 56
BstYI r'GATC_y
Cuts at: 1972 3709 4076 4461 4478 5930 6016 6028 6374
Size: 1737 367 385 17 1452 86 12 346
Cuts at: 6374 1972
Size: 1977
Fragments arranged by size:
1977 1737 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3318 3318
Size: 6379
BtgI C'CryG_G
Cuts at: 513 1065 2004 2994 3309 513
Size: 552 939 990 315 3583
Fragments arranged by size:
3583 990 939 552 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5640
Fragments arranged by size:
5640 683 56
BtsI GCAGTG_nn'
Cuts at: 2112 3081 3425 3631 3895 4689 4709 2112
Size: 969 344 206 264 794 20 3782
Fragments arranged by size:
3782 969 794 344 264 206 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6344
DraI TTT'AAA
Cuts at: 3683 3683
Size: 6379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5386 809
Size: 507 4070 1802
Fragments arranged by size:
4070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2353 2471
Size: 54 1425 50 275 18 252 271 118
Cuts at: 2471 3826 4715 6344 8
Size: 1355 889 1629 43
Fragments arranged by size:
1629 1425 1355 889 275 271 252 118
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3826 1537
Size: 275 2014 4090
Fragments arranged by size:
4090 2014 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2736 3474 4309 1353
Size: 1383 738 835 3423
Fragments arranged by size:
3423 1383 835 738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4966 5340 5486 387
Size: 1064 138 3377 374 146 1280
Fragments arranged by size:
3377 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2183 2406 3208 3377 3464 3562
Size: 393 549 2 223 802 169 87 98
Cuts at: 3562 4442 5039 5826 6094 1239
Size: 880 597 787 268 1524
Fragments arranged by size:
1524 880 802 787 597 549 393 268
223 169 98 87 2
EcoO109I rG'GnC_Cy
Cuts at: 2033 2076 2077 2524 2525 2698 3193 3263 2033
Size: 43 1 447 1 173 495 70 5149
Fragments arranged by size:
5149 495 447 173 70 43 1 1
EcoRI G'AATT_C
Cuts at: 3688 4066 3688
Size: 378 6001
FspI TGC'GCA
Cuts at: 4885 4885
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 2659 2758 5526 1678
Size: 981 99 2768 2531
Fragments arranged by size:
2768 2531 981 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2653 2685 2907 2939 3205
Size: 32 135 32 1081 32 222 32 266
Cuts at: 3205 3237 3314 3346 5020 5052 5094 5126 1373
Size: 32 77 32 1674 32 42 32 2626
Fragments arranged by size:
2626 1674 1081 266 222 135 77 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2479 3723 3969 669
Size: 988 281 541 1244 246 3079
Fragments arranged by size:
3079 1244 988 541 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6379
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KasI G'GCGC_C
Cuts at: 2655 2754 2655
Size: 99 6280
KpnI G_GTAC'C
Cuts at: 3704 3704
Size: 6379
MfeI C'AATT_G
Cuts at: 3978 3978
Size: 6379
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3357 3405 5493 5677 1305
Size: 2052 48 2088 184 2007
Fragments arranged by size:
2088 2052 2007 184 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2473 10
Size: 54 1425 343 641 3916
Fragments arranged by size:
3916 1425 641 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2708 2804 2963 2999 4337 4696 4855
Size: 648 1542 96 159 36 1338 359 159
Cuts at: 4855 518
Size: 2042
Fragments arranged by size:
2042 1542 1338 648 359 159 159 96
36
MspA1I CmG'CkG
Cuts at: 1606 2358 2825 3186 3247 3442 3583 3795 4472
Size: 752 467 361 61 195 141 212 677
Cuts at: 4472 5620 5865 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 752 677 467 361 245 212
195 141 61
NarI GG'CG_CC
Cuts at: 2656 2755 2656
Size: 99 6280
NcoI C'CATG_G
Cuts at: 513 1065 2004 2994 513
Size: 552 939 990 3898
Fragments arranged by size:
3898 990 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6379
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 2174 3737 5282 1132
Size: 97 945 1563 1545 2229
Fragments arranged by size:
2229 1563 1545 945 97
PasI CC'CwG_GG
Cuts at: 1348 2833 1348
Size: 1485 4894
PciI A'CATG_T
Cuts at: 5278 5278
Size: 6379
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2133 3028 1108
Size: 1025 895 4459
Fragments arranged by size:
4459 1025 895
PfoI T'CCnGG_A
Cuts at: 3129 3129
Size: 6379
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PmlI CAC'GTG
Cuts at: 3090 3090
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4448 4480 5961 5993 4448
Size: 32 1481 32 4834
Fragments arranged by size:
4834 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3263 3263
Size: 6379
PshAI GACnn'nnGTC
Cuts at: 1613 3489 1613
Size: 1876 4503
PsiI TTA'TAA
Cuts at: 3949 3949
Size: 6379
PspOMI G'GGCC_C
Cuts at: 2076 2524 3527 2076
Size: 448 1003 4928
Fragments arranged by size:
4928 1003 448
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2366 2398 2366
Size: 32 6347
PstI C_TGCA'G
Cuts at: 830 2694 2721 3369 3731 830
Size: 1864 27 648 362 3478
Fragments arranged by size:
3478 1864 648 362 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
PvuII CAG'CTG
Cuts at: 2825 3186 3247 3442 3583 2825
Size: 361 61 195 141 5621
Fragments arranged by size:
5621 361 195 141 61
SalI G'TCGA_C
Cuts at: 1936 2477 3721 1936
Size: 541 1244 4594
Fragments arranged by size:
4594 1244 541
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 4627 6339 4627
Size: 1712 4667
SexAI A'CCwGG_T
Cuts at: 2634 3150 2634
Size: 516 5863
SfcI C'TryA_G
Cuts at: 826 1045 2690 2717 3365 3727 4862 5543 5734
Size: 219 1645 27 648 362 1135 681 191
Cuts at: 5734 826
Size: 1471
Fragments arranged by size:
1645 1471 1135 681 648 362 219 191
27
SfoI GGC'GCC
Cuts at: 2657 2756 2657
Size: 99 6280
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 3383 3706 1872
Size: 1511 323 4545
Fragments arranged by size:
4545 1511 323
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2266 2517 4483 5384 5646 820
Size: 197 926 323 251 1966 901 262 1553
Fragments arranged by size:
1966 1553 926 901 323 262 251 197
SnaBI TAC'GTA
Cuts at: 493 2142 4170 493
Size: 1649 2028 2702
Fragments arranged by size:
2702 2028 1649
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 3737 1132
Size: 2605 3774
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 3431 1517
Size: 1914 4465
StyI C'CwwG_G
Cuts at: 513 1065 2004 2029 2650 2994 3159 3288 3818
Size: 552 939 25 621 344 165 129 530
Cuts at: 3818 513
Size: 3074
Fragments arranged by size:
3074 939 621 552 530 344 165 129
25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4406 4423 4576 4761 5206 325
Size: 1255 2826 17 153 185 445 1498
Fragments arranged by size:
2826 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2250 3268 4625
Size: 274 80 33 51 1443 273 1018 1357
Cuts at: 4625 6337 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1357 1018 274 273 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3233 3265 3298 3330 6339 6371 3233
Size: 32 33 32 3009 32 3241
Fragments arranged by size:
3241 3009 33 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2635 3001 3025 2635
Size: 366 24 5989
Fragments arranged by size:
5989 366 24
XhoI C'TCGA_G
Cuts at: 1943 2517 1943
Size: 574 5805
XmaI C'CCGG_G
Cuts at: 1870 3381 3704 1870
Size: 1511 323 4545
Fragments arranged by size:
4545 1511 323
XmnI GAAnn'nnTTC
Cuts at: 4508 4508
Size: 6379
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbvCI BfrBI Bpu10I BsiWI BstZ17I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI RsrII SacI SacII
SanDI SapI SfiI SrfI SwaI