Restriction map for
pFN21AE1031
(Circular) (Six-base) MAPSORT of: pFN21AE1031.seq Check: 7972 from: 1 to: 8884
pFN21AE1031 8884 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4102 4872 4102
Size: 770 8114
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5621 6694 278
Size: 53 83 186 936 4085 1073 2468
Fragments arranged by size:
4085 2468 1073 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6227 1937
Size: 4290 4594
Acc65I G'GTAC_C
Cuts at: 3459 6205 3459
Size: 2746 6138
AclI AA'CG_TT
Cuts at: 1563 7011 7384 1563
Size: 5448 373 3063
Fragments arranged by size:
5448 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2511 3071 3585 5328 5840 5856 6947 8331
Size: 879 560 514 1743 512 16 1091 1384
Cuts at: 8331 8599 1632
Size: 268 1917
Fragments arranged by size:
1917 1743 1384 1091 879 560 514 512
268 16
AfeI AGC'GCT
Cuts at: 4905 4905
Size: 8884
AflII C'TTAA_G
Cuts at: 820 1017 3307 820
Size: 197 2290 6397
Fragments arranged by size:
6397 2290 197
AflIII A'CryG_T
Cuts at: 1203 5758 7783 8760 1203
Size: 4555 2025 977 1327
Fragments arranged by size:
4555 2025 1327 977
AgeI A'CCGG_T
Cuts at: 7715 7715
Size: 8884
AhdI GACnn_n'nnGTC
Cuts at: 3605 5089 7613 3605
Size: 1484 2524 4876
Fragments arranged by size:
4876 2524 1484
AleI CACnn'nnGTG
Cuts at: 1166 5902 1166
Size: 4736 4148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2328 5408 6020 1184
Size: 1144 3080 612 4048
Fragments arranged by size:
4048 3080 1144 612
AlwNI CAG_nnn'CTG
Cuts at: 2834 3187 3680 4873 4915 5230 5536 6145 6627
Size: 353 493 1193 42 315 306 609 482
Cuts at: 6627 8199 2834
Size: 1572 3519
Fragments arranged by size:
3519 1572 1193 609 493 482 353 315
306 42
ApaI G_GGCC'C
Cuts at: 2504 2537 3268 4382 4846 5695 2504
Size: 33 731 1114 464 849 5693
Fragments arranged by size:
5693 1114 849 731 464 33
ApaLI G'TGCA_C
Cuts at: 6941 8097 6941
Size: 1156 7728
ApoI r'AATT_y
Cuts at: 1493 6193 6419 6571 8680 1493
Size: 4700 226 152 2109 1697
Fragments arranged by size:
4700 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7438 160
Size: 872 6406 1606
Fragments arranged by size:
6406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8884
AvaI C'yCGr_G
Cuts at: 1870 1943 4630 4768 5202 5356 6209 1870
Size: 73 2687 138 434 154 853 4545
Fragments arranged by size:
4545 2687 853 434 154 138 73
AvrII C'CTAG_G
Cuts at: 2573 2573
Size: 8884
BamHI G'GATC_C
Cuts at: 6214 6581 6214
Size: 367 8517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2546 3459 4117 5430 5904
Size: 325 217 627 759 913 658 1313 474
Cuts at: 5904 6205 7660 618
Size: 301 1455 1842
Fragments arranged by size:
1842 1455 1313 913 759 658 627 474
325 301 217
BanII G_rGCy'C
Cuts at: 1391 2504 2537 2649 2913 3268 3409 3438 3621
Size: 1113 33 112 264 355 141 29 183
Cuts at: 3621 4086 4131 4305 4382 4846 5695 5779 5975
Size: 465 45 174 77 464 849 84 196
Cuts at: 5975 6102 6203 1391
Size: 127 101 4072
Fragments arranged by size:
4072 1113 849 465 464 355 264 196
183 174 141 127 112 101 84 77
45 33 29
BbeI G_GCGC'C
Cuts at: 5434 5434
Size: 8884
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3077 5844 928
Size: 971 1178 2767 3968
Fragments arranged by size:
3968 2767 1178 971
BbvCI CC'TCA_GC
Cuts at: 2288 3892 3966 4248 2288
Size: 1604 74 282 6924
Fragments arranged by size:
6924 1604 282 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 7075 7109 1903
Size: 34 50 34 5054 34 3678
Fragments arranged by size:
5054 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6776 7986 6776
Size: 1210 7674
BclI T'GATC_A
Cuts at: 1548 2299 1548
Size: 751 8133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4760 5225 5471
Size: 107 122 71 631 605 3088 465 246
Cuts at: 5471 7495 136
Size: 2024 1525
Fragments arranged by size:
3088 2024 1525 631 605 465 246 122
107 71
BglII A'GATC_T
Cuts at: 2792 8879 2792
Size: 6087 2797
BlpI GC'TnA_GC
Cuts at: 2664 4013 4130 4663 4964 5012 6301 2664
Size: 1349 117 533 301 48 1289 5247
Fragments arranged by size:
5247 1349 1289 533 301 117 48
Bme1580I G_kGCm'C
Cuts at: 1790 2376 2504 2526 2537 3268 3764 4382 4389
Size: 586 128 22 11 731 496 618 7
Cuts at: 4389 4846 5387 5457 5695 5909 6945 8101 1790
Size: 457 541 70 238 214 1036 1156 2573
Fragments arranged by size:
2573 1156 1036 731 618 586 541 496
457 238 214 128 70 22 11 7
BmgBI CAC'GTC
Cuts at: 1318 3112 1318
Size: 1794 7090
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2370 4085 4376
Size: 416 53 83 399 24 953 1715 291
Cuts at: 4376 4535 4631 5519 5791 6615 7573 442
Size: 159 96 888 272 824 958 1753
Fragments arranged by size:
1753 1715 958 953 888 824 416 399
291 272 159 96 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3296 1056
Size: 2240 6644
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2848 5683 5805 2848
Size: 2835 122 5927
Fragments arranged by size:
5927 2835 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2951 3026 3444 3680 3776 4289 4450
Size: 899 813 75 418 236 96 513 161
Cuts at: 4450 4451 4760 4775 5051 5834 6122 7544 1239
Size: 1 309 15 276 783 288 1422 2579
Fragments arranged by size:
2579 1422 899 813 783 513 418 309
288 276 236 161 96 75 15 1
Bpu10I CC'TnA_GC
Cuts at: 2288 2648 3892 3966 4248 5142 2288
Size: 360 1244 74 282 894 6030
Fragments arranged by size:
6030 1244 894 360 282 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2809 5556 7009 7874 8172 2809
Size: 2747 1453 865 298 3521
Fragments arranged by size:
3521 2747 1453 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2008 3816 6044 6173 7547 882
Size: 617 509 1808 2228 129 1374 2219
Fragments arranged by size:
2228 2219 1808 1374 617 509 129
BsaAI yAC'GTr
Cuts at: 493 1204 3655 5761 6675 493
Size: 711 2451 2106 914 2702
Fragments arranged by size:
2702 2451 2106 914 711
BsaBI GATnn'nnATC
Cuts at: 8878 8878
Size: 8884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5431 5618 6691 7073
Size: 53 83 186 936 3898 187 1073 382
Cuts at: 7073 7708 275
Size: 635 1451
Fragments arranged by size:
3898 1451 1073 936 635 382 187 186
83 53
BsaWI w'CCGG_w
Cuts at: 3939 5868 7317 7715 7989 8136 8547 3939
Size: 1929 1449 398 274 147 411 4276
Fragments arranged by size:
4276 1929 1449 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2554 2584 4469 4499 5815
Size: 30 165 30 1151 30 1885 30 1316
Cuts at: 5815 5845 1178
Size: 30 4217
Fragments arranged by size:
4217 1885 1316 1151 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2211 2305 2741 2852 3167 3428 3446 4178
Size: 1025 94 436 111 315 261 18 732
Cuts at: 4178 4505 4685 5540 1186
Size: 327 180 855 4530
Fragments arranged by size:
4530 1025 855 732 436 327 315 261
180 111 94 18
BseYI C'CCAG_C
Cuts at: 2086 2203 2240 2760 2771 2944 3216 3491 3535
Size: 117 37 520 11 173 272 275 44
Cuts at: 3535 3896 4194 4607 5162 5556 8087 8583 2086
Size: 361 298 413 555 394 2531 496 2387
Fragments arranged by size:
2531 2387 555 520 496 413 394 361
298 275 272 173 117 44 37 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2100 2114 2969 3122 5216 6041
Size: 573 297 67 14 855 153 2094 825
Cuts at: 6041 1163
Size: 4006
Fragments arranged by size:
4006 2094 855 825 573 297 153 67
14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6334 7095 8123 1540
Size: 275 186 4333 761 1028 2301
Fragments arranged by size:
4333 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3229 3400 3438 3711 4019 4086 4131 5779 5916
Size: 171 38 273 308 67 45 1648 137
Cuts at: 5916 5975 6102 6162 6945 7030 8101 3229
Size: 59 127 60 783 85 1071 4012
Fragments arranged by size:
4012 1648 1071 783 308 273 171 137
127 85 67 60 59 45 38
BsmI GAATG_Cn'
Cuts at: 3536 3727 6395 6488 3536
Size: 191 2668 93 5932
Fragments arranged by size:
5932 2668 191 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4652 4652
Size: 8884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2376 2504 2526 2537 2649 2913 3229
Size: 399 586 128 22 11 112 264 316
Cuts at: 3229 3268 3400 3409 3438 3621 3711 3764 4019
Size: 39 132 9 29 183 90 53 255
Cuts at: 4019 4086 4131 4305 4382 4389 4846 5387 5457
Size: 67 45 174 77 7 457 541 70
Cuts at: 5457 5695 5779 5909 5916 5975 6102 6162 6203
Size: 238 84 130 7 59 127 60 41
Cuts at: 6203 6945 7030 8101 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 586 541 457 399 316
264 255 238 183 174 132 130 128
127 112 90 85 84 77 70 67
60 59 53 45 41 39 29 22
11 9 7 7
BspEI T'CCGG_A
Cuts at: 3939 5868 8547 3939
Size: 1929 2679 4276
Fragments arranged by size:
4276 2679 1929
BspHI T'CATG_A
Cuts at: 6773 8503 6773
Size: 1730 7154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 3126 3688 4102 4872 6239 844
Size: 1208 1074 562 414 770 1367 3489
Fragments arranged by size:
3489 1367 1208 1074 770 562 414
BsrBI CCG'CTC
Cuts at: 1394 1823 2152 3167 6771 1394
Size: 429 329 1015 3604 3507
Fragments arranged by size:
3604 3507 1015 429 329
BsrDI GCAATG_nn'
Cuts at: 65 1248 2954 3378 5188 7379 7553 65
Size: 1183 1706 424 1810 2191 174 1396
Fragments arranged by size:
2191 1810 1706 1396 1183 424 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2142 3288 3576 4211 4968 7528 7715
Size: 110 332 1146 288 635 757 2560 187
Cuts at: 7715 1700
Size: 2869
Fragments arranged by size:
2869 2560 1146 757 635 332 288 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8884
BssSI C'ACGA_G
Cuts at: 4613 6944 7956 4613
Size: 2331 1012 5541
Fragments arranged by size:
5541 2331 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2078 2752 4117 4915 5410 6055 794
Size: 1284 674 1365 798 495 645 3623
Fragments arranged by size:
3623 1365 1284 798 674 645 495
BstBI TT'CG_AA
Cuts at: 7688 7688
Size: 8884
BstEII G'GTnAC_C
Cuts at: 1184 4067 1184
Size: 2883 6001
BstXI CCAn_nnnn'nTGG
Cuts at: 2597 3130 5215 5647 5687 2597
Size: 533 2085 432 40 5794
Fragments arranged by size:
5794 2085 533 432 40
BstYI r'GATC_y
Cuts at: 1972 2730 2792 4722 6214 6581 6966 6983 8435
Size: 758 62 1930 1492 367 385 17 1452
Cuts at: 8435 8521 8533 8879 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1930 1492 1452 758 385 367 346
86 62 17 12
Bsu36I CC'TnA_GG
Cuts at: 2262 2724 4547 2262
Size: 462 1823 6599
Fragments arranged by size:
6599 1823 462
BtgI C'CryG_G
Cuts at: 513 1065 2715 3145 3463 4121 4374 5379 513
Size: 552 1650 430 318 658 253 1005 4018
Fragments arranged by size:
4018 1650 1005 658 552 430 318 253
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8145
Fragments arranged by size:
8145 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 2251 2310 3233 3307 4175 4505 4552 4686
Size: 154 59 923 74 868 330 47 134
Cuts at: 4686 4913 5411 6400 7194 7214 2097
Size: 227 498 989 794 20 3767
Fragments arranged by size:
3767 989 923 868 794 498 330 227
154 134 74 59 47 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2334 2369 529
Size: 35 1770 35 7044
Fragments arranged by size:
7044 1770 35 35
DraI TTT'AAA
Cuts at: 6188 6188
Size: 8884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4294 7891 809
Size: 507 2978 3597 1802
Fragments arranged by size:
3597 2978 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2248 2355 4057
Size: 54 1425 50 275 18 418 107 1702
Cuts at: 4057 4263 4741 6331 7220 8849 8
Size: 206 478 1590 889 1629 43
Fragments arranged by size:
1702 1629 1590 1425 889 478 418 275
206 107 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6331 1537
Size: 275 4519 4090
Fragments arranged by size:
4519 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2274 2685 2912 3341 3405 4707 6814 1353
Size: 921 411 227 429 64 1302 2107 3423
Fragments arranged by size:
3423 2107 1302 921 429 411 227 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3704 5882 7471 7845 7991 387
Size: 1064 138 2115 2178 1589 374 146 1280
Fragments arranged by size:
2178 2115 1589 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3436 4084 4129 5777 5973 6100 3436
Size: 648 45 1648 196 127 6220
Fragments arranged by size:
6220 1648 648 196 127 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2511 2951 3026 3071 3444 3585
Size: 393 506 373 440 75 45 373 141
Cuts at: 3585 3680 3776 4289 4450 4451 4760 4775 5051
Size: 95 96 513 161 1 309 15 276
Cuts at: 5051 5328 5834 5840 5856 6122 6947 7544 8331
Size: 277 506 6 16 266 825 597 787
Cuts at: 8331 8599 1239
Size: 268 1524
Fragments arranged by size:
1524 825 787 597 513 506 506 440
393 373 373 309 277 276 268 266
161 141 96 95 75 45 16 15
6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 4487 2261
Size: 2226 6658
EcoO109I rG'GnC_Cy
Cuts at: 2272 2500 2621 2855 3264 3677 3751 3827 4024
Size: 228 121 234 409 413 74 76 197
Cuts at: 4024 4843 5155 5398 5691 5692 5962 2272
Size: 819 312 243 293 1 270 5194
Fragments arranged by size:
5194 819 413 409 312 293 270 243
234 228 197 121 76 74 1
EcoRI G'AATT_C
Cuts at: 6193 6571 6193
Size: 378 8506
FspI TGC'GCA
Cuts at: 7390 7390
Size: 8884
HaeII r_GCGC'y
Cuts at: 1678 4483 4907 5434 8031 1678
Size: 2805 424 527 2597 2531
Fragments arranged by size:
2805 2597 2531 527 424
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2284 2316 2554
Size: 32 135 32 548 32 132 32 238
Cuts at: 2554 2586 3058 3090 3466 3498 3591 3623 4469
Size: 32 472 32 376 32 93 32 846
Cuts at: 4469 4501 5075 5107 5339 5371 7525 7557 7599
Size: 32 574 32 232 32 2154 32 42
Cuts at: 7599 7631 1373
Size: 32 2626
Fragments arranged by size:
2626 2154 846 574 548 472 376 238
232 135 132 93 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6228 6474 669
Size: 988 281 4290 246 3079
Fragments arranged by size:
4290 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6065 748
Size: 5317 3567
HpaI GTT'AAC
Cuts at: 6474 6474
Size: 8884
KasI G'GCGC_C
Cuts at: 5430 5430
Size: 8884
KpnI G_GTAC'C
Cuts at: 3463 6209 3463
Size: 2746 6138
MfeI C'AATT_G
Cuts at: 6483 6483
Size: 8884
MluI A'CGCG_T
Cuts at: 8760 8760
Size: 8884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2160 7998 8182 1305
Size: 855 5838 184 2007
Fragments arranged by size:
5838 2007 855 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2250 4059 4743 10
Size: 54 1425 343 418 1809 684 4151
Fragments arranged by size:
4151 1809 1425 684 418 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2166 3144 5390 5486 5902 6842 7201
Size: 648 1000 978 2246 96 416 940 359
Cuts at: 7201 7360 518
Size: 159 2042
Fragments arranged by size:
2246 2042 1000 978 940 648 416 359
159 96
MspA1I CmG'CkG
Cuts at: 1606 2075 2098 2749 3896 4123 4165 4576 4747
Size: 469 23 651 1147 227 42 411 171
Cuts at: 4747 5089 5290 6300 6977 8125 8370 1606
Size: 342 201 1010 677 1148 245 2120
Fragments arranged by size:
2120 1148 1147 1010 677 651 469 411
342 245 227 201 171 42 23
NaeI GCC'GGC
Cuts at: 3578 4213 4970 3578
Size: 635 757 7492
Fragments arranged by size:
7492 757 635
NarI GG'CG_CC
Cuts at: 5431 5431
Size: 8884
NcoI C'CATG_G
Cuts at: 513 1065 2715 3145 3463 5379 513
Size: 552 1650 430 318 1916 4018
Fragments arranged by size:
4018 1916 1650 552 430 318
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8884
NgoMIV G'CCGG_C
Cuts at: 3576 4211 4968 3576
Size: 635 757 7492
Fragments arranged by size:
7492 757 635
NheI G'CTAG_C
Cuts at: 1052 3292 1052
Size: 2240 6644
NotI GC'GGCC_GC
Cuts at: 6331 6331
Size: 8884
NspI r_CATG'y
Cuts at: 1132 1229 3242 6242 7787 1132
Size: 97 2013 3000 1545 2229
Fragments arranged by size:
3000 2229 2013 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8884
PciI A'CATG_T
Cuts at: 7783 7783
Size: 8884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2510 3807 3982 4474 4798 5304 1108
Size: 1402 1297 175 492 324 506 4688
Fragments arranged by size:
4688 1402 1297 506 492 324 175
PfoI T'CCnGG_A
Cuts at: 2154 3421 3753 4467 5040 5514 5646 2154
Size: 1267 332 714 573 474 132 5392
Fragments arranged by size:
5392 1267 714 573 474 332 132
PmeI GTTT'AAAC
Cuts at: 6188 6188
Size: 8884
PmlI CAC'GTG
Cuts at: 5761 5761
Size: 8884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6953 6985 8466 8498 6953
Size: 32 1481 32 7339
Fragments arranged by size:
7339 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2272 2621 2855 3677 3751 5155 5398 5962 2272
Size: 349 234 822 74 1404 243 564 5194
Fragments arranged by size:
5194 1404 822 564 349 243 234 74
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8884
PsiI TTA'TAA
Cuts at: 6454 6454
Size: 8884
PspOMI G'GGCC_C
Cuts at: 2500 2533 3264 4378 4842 5691 2500
Size: 33 731 1114 464 849 5693
Fragments arranged by size:
5693 1114 849 731 464 33
PstI C_TGCA'G
Cuts at: 830 2712 2739 4869 4911 5406 5715 6012 6236
Size: 1882 27 2130 42 495 309 297 224
Cuts at: 6236 830
Size: 3478
Fragments arranged by size:
3478 2130 1882 495 309 297 224 42
27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8884
PvuII CAG'CTG
Cuts at: 2075 2098 3896 4747 5089 2075
Size: 23 1798 851 342 5870
Fragments arranged by size:
5870 1798 851 342 23
SacI G_AGCT'C
Cuts at: 3438 4086 4131 5779 5975 6102 3438
Size: 648 45 1648 196 127 6220
Fragments arranged by size:
6220 1648 648 196 127 45
SacII CC_GC'GG
Cuts at: 4124 4124
Size: 8884
SalI G'TCGA_C
Cuts at: 1936 6226 1936
Size: 4290 4594
SanDI GG'GwC_CC
Cuts at: 2621 2621
Size: 8884
SapI GCTCTTCn'nnn_
Cuts at: 3405 3405
Size: 8884
SbfI CC_TGCA'GG
Cuts at: 2739 6236 2739
Size: 3497 5387
ScaI AGT'ACT
Cuts at: 7132 8844 7132
Size: 1712 7172
SfcI C'TryA_G
Cuts at: 826 1045 2708 2735 3179 3780 4865 4907 5246
Size: 219 1663 27 444 601 1085 42 339
Cuts at: 5246 5402 5711 6008 6232 7367 8048 8239 826
Size: 156 309 297 224 1135 681 191 1471
Fragments arranged by size:
1663 1471 1135 1085 681 601 444 339
309 297 224 219 191 156 42 27
SfoI GGC'GCC
Cuts at: 5432 5432
Size: 8884
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8884
SmaI CCC'GGG
Cuts at: 1872 4770 6211 1872
Size: 2898 1441 4545
Fragments arranged by size:
4545 2898 1441
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2788 3307 5571 6988 7889 8151
Size: 197 926 845 519 2264 1417 901 262
Cuts at: 8151 820
Size: 1553
Fragments arranged by size:
2264 1553 1417 926 901 845 519 262
197
SnaBI TAC'GTA
Cuts at: 493 6675 493
Size: 6182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8884
SphI G_CATG'C
Cuts at: 1132 6242 1132
Size: 5110 3774
SspI AAT'ATT
Cuts at: 5 52 3907 6808 5
Size: 47 3855 2901 2081
Fragments arranged by size:
3855 2901 2081 47
StuI AGG'CCT
Cuts at: 1517 4427 5147 1517
Size: 2910 720 5254
Fragments arranged by size:
5254 2910 720
StyI C'CwwG_G
Cuts at: 513 1065 2496 2573 2715 2874 3145 3354 3463
Size: 552 1431 77 142 159 271 209 109
Cuts at: 3463 4953 5379 6323 513
Size: 1490 426 944 3074
Fragments arranged by size:
3074 1490 1431 944 552 426 271 209
159 142 109 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2032 2581 3140 4151 4155 4208 4940
Size: 1255 452 549 559 1011 4 53 732
Cuts at: 4940 5012 5398 6911 6928 7081 7266 7711 325
Size: 72 386 1513 17 153 185 445 1498
Fragments arranged by size:
1513 1498 1255 1011 732 559 549 452
445 386 185 153 72 53 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5527 7130 8842
Size: 274 80 33 51 1443 3550 1603 1712
Cuts at: 8842 96
Size: 138
Fragments arranged by size:
3550 1712 1603 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3669 3701 5815 5847 8844 8876 3669
Size: 32 2114 32 2997 32 3677
Fragments arranged by size:
3677 2997 2114 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2996 1316
Size: 1680 7204
XbaI T'CTAG_A
Cuts at: 6220 6220
Size: 8884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3751 5377 3751
Size: 1626 7258
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8884
XmaI C'CCGG_G
Cuts at: 1870 4768 6209 1870
Size: 2898 1441 4545
Fragments arranged by size:
4545 2898 1441
XmnI GAAnn'nnTTC
Cuts at: 4784 7013 4784
Size: 2229 6655
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5619 6692 276
Size: 53 83 186 936 4085 1073 2468
Fragments arranged by size:
4085 2468 1073 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SexAI SfiI SrfI SwaI