Restriction map for
pFN21AE1026
(Circular) (Six-base) MAPSORT of: pFN21AE1026.seq Check: 7344 from: 1 to: 7642
pFN21AE1026 7642 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2404 2596 2845 2404
Size: 192 249 7201
Fragments arranged by size:
7201 249 192
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3870 5452 278
Size: 53 83 186 936 2334 1582 2468
Fragments arranged by size:
2468 2334 1582 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2021 3864 4985 1937
Size: 84 1843 1121 4594
Fragments arranged by size:
4594 1843 1121 84
Acc65I G'GTAC_C
Cuts at: 2597 4963 2597
Size: 2366 5276
AclI AA'CG_TT
Cuts at: 1563 5769 6142 1563
Size: 4206 373 3063
Fragments arranged by size:
4206 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2343 5705 7089 7357 1632
Size: 711 3362 1384 268 1917
Fragments arranged by size:
3362 1917 1384 711 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7445
AflIII A'CryG_T
Cuts at: 1203 2475 2869 2871 3081 4257 4659 6541 7518
Size: 1272 394 2 210 1176 402 1882 977
Cuts at: 7518 1203
Size: 1327
Fragments arranged by size:
1882 1327 1272 1176 977 402 394 210
2
AgeI A'CCGG_T
Cuts at: 2267 6473 2267
Size: 4206 3436
AhdI GACnn_n'nnGTC
Cuts at: 6371 6371
Size: 7642
AleI CACnn'nnGTG
Cuts at: 1166 3242 3893 1166
Size: 2076 651 4915
Fragments arranged by size:
4915 2076 651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3806 1184
Size: 2622 5020
AlwNI CAG_nnn'CTG
Cuts at: 2437 2536 2698 2797 3418 3835 4075 4246 4327
Size: 99 162 99 621 417 240 171 81
Cuts at: 4327 4414 4930 5385 6957 2437
Size: 87 516 455 1572 3122
Fragments arranged by size:
3122 1572 621 516 455 417 240 171
162 99 99 87 81
ApaI G_GGCC'C
Cuts at: 2674 3810 4865 2674
Size: 1136 1055 5451
Fragments arranged by size:
5451 1136 1055
ApaLI G'TGCA_C
Cuts at: 2271 2609 5699 6855 2271
Size: 338 3090 1156 3058
Fragments arranged by size:
3090 3058 1156 338
ApoI r'AATT_y
Cuts at: 1493 4951 5177 5329 7438 1493
Size: 3458 226 152 2109 1697
Fragments arranged by size:
3458 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6196 160
Size: 872 5164 1606
Fragments arranged by size:
5164 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 3809 4193 4967 1870
Size: 73 1014 852 384 774 4545
Fragments arranged by size:
4545 1014 852 774 384 73
BamHI G'GATC_C
Cuts at: 4972 5339 4972
Size: 367 7275
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 2327 2423 2597 2857
Size: 325 217 627 326 214 96 174 260
Cuts at: 2857 3236 3494 3949 4229 4963 6418 618
Size: 379 258 455 280 734 1455 1842
Fragments arranged by size:
1842 1455 734 627 455 379 326 325
280 260 258 217 214 174 96
BanII G_rGCy'C
Cuts at: 1391 2186 2238 2674 3426 3771 3810 4029 4865
Size: 795 52 436 752 345 39 219 836
Cuts at: 4865 4961 1391
Size: 96 4072
Fragments arranged by size:
4072 836 795 752 436 345 219 96
52 39
BbeI G_GCGC'C
Cuts at: 3953 4233 3953
Size: 280 7362
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3457 3917 928
Size: 971 1558 460 4653
Fragments arranged by size:
4653 1558 971 460
BbvCI CC'TCA_GC
Cuts at: 2154 3351 3932 2154
Size: 1197 581 5864
Fragments arranged by size:
5864 1197 581
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3079 3113 5833 5867 1903
Size: 34 1142 34 2720 34 3678
Fragments arranged by size:
3678 2720 1142 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5534 6744 5534
Size: 1210 6432
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7642
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2336 2388 3492
Size: 107 122 71 631 605 664 52 1104
Cuts at: 3492 4739 4892 6253 136
Size: 1247 153 1361 1525
Fragments arranged by size:
1525 1361 1247 1104 664 631 605 153
122 107 71 52
BglII A'GATC_T
Cuts at: 2744 7637 2744
Size: 4893 2749
BlpI GC'TnA_GC
Cuts at: 4328 5059 4328
Size: 731 6911
Bme1580I G_kGCm'C
Cuts at: 1790 2275 2426 2511 2613 2674 2684 2862 3497
Size: 485 151 85 102 61 10 178 635
Cuts at: 3497 3810 4865 5703 6859 1790
Size: 313 1055 838 1156 2573
Fragments arranged by size:
2573 1156 1055 838 635 485 313 178
151 102 85 61 10
BmgBI CAC'GTC
Cuts at: 1318 4099 4260 1318
Size: 2781 161 4700
Fragments arranged by size:
4700 2781 161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3129 3936 4110
Size: 416 53 83 399 24 1712 807 174
Cuts at: 4110 5373 6331 442
Size: 1263 958 1753
Fragments arranged by size:
1753 1712 1263 958 807 416 399 174
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7642
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4390 4390
Size: 7642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2471 2798 2834 2863 3440 3761 3940 4178
Size: 1232 327 36 29 577 321 179 238
Cuts at: 4178 4436 4478 4895 4921 6302 1239
Size: 258 42 417 26 1381 2579
Fragments arranged by size:
2579 1381 1232 577 417 327 321 258
238 179 42 36 29 26
Bpu10I CC'TnA_GC
Cuts at: 2154 3351 3932 2154
Size: 1197 581 5864
Fragments arranged by size:
5864 1197 581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2817 3608 4487 4541 5767 6632 6930 2817
Size: 791 879 54 1226 865 298 3529
Fragments arranged by size:
3529 1226 879 865 791 298 54
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2584 4697 4862 6305 882
Size: 617 1085 2113 165 1443 2219
Fragments arranged by size:
2219 2113 1443 1085 617 165
BsaAI yAC'GTr
Cuts at: 493 1204 2227 2476 2827 2872 3082 3244 5433
Size: 711 1023 249 351 45 210 162 2189
Cuts at: 5433 493
Size: 2702
Fragments arranged by size:
2702 2189 1023 711 351 249 210 162
45
BsaBI GATnn'nnATC
Cuts at: 7636 7636
Size: 7642
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2712 2963 3801 3867
Size: 53 83 186 936 1179 251 838 66
Cuts at: 3867 3950 4230 5449 5831 6466 275
Size: 83 280 1219 382 635 1451
Fragments arranged by size:
1451 1219 1179 936 838 635 382 280
251 186 83 83 66 53
BsaWI w'CCGG_w
Cuts at: 2267 3212 4137 6075 6473 6747 6894 7305 2267
Size: 945 925 1938 398 274 147 411 2604
Fragments arranged by size:
2604 1938 945 925 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2861 2891 3422 3452 3890
Size: 30 165 30 1458 30 531 30 438
Cuts at: 3890 3920 1178
Size: 30 4900
Fragments arranged by size:
4900 1458 531 438 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4001 4211 4544 4793 4805 4808 4811 1186
Size: 2815 210 333 249 12 3 3 4017
Fragments arranged by size:
4017 2815 333 249 210 12 3 3
BseYI C'CCAG_C
Cuts at: 2052 2079 2085 2107 2118 2187 2295 2427 2511
Size: 27 6 22 11 69 108 132 84
Cuts at: 2511 2601 3125 3265 4858 6845 7341 2052
Size: 90 524 140 1593 1987 496 2353
Fragments arranged by size:
2353 1987 1593 524 496 140 132 108
90 84 69 27 22 11 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2274 2438 2477 2564 2657 2810 2825
Size: 573 538 164 39 87 93 153 15
Cuts at: 2825 2861 2909 3383 3734 4382 4427 4943 1163
Size: 36 48 474 351 648 45 516 3862
Fragments arranged by size:
3862 648 573 538 516 474 351 164
153 93 87 48 45 39 36 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5092 5853 6881 1540
Size: 275 186 3091 761 1028 2301
Fragments arranged by size:
3091 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 2275 2613 3377 3686 3771 4011 4035 4503
Size: 37 338 764 309 85 240 24 468
Cuts at: 4503 4680 5703 5788 6859 2238
Size: 177 1023 85 1071 3021
Fragments arranged by size:
3021 1071 1023 764 468 338 309 240
177 85 85 37 24
BsmI GAATG_Cn'
Cuts at: 5153 5246 5153
Size: 93 7549
BsmBI CGTCTCn'nnnn_
Cuts at: 2954 3338 3893 2954
Size: 384 555 6703
Fragments arranged by size:
6703 555 384
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2238 2275 2426 2511 2613 2674
Size: 399 396 52 37 151 85 102 61
Cuts at: 2674 2684 2862 3377 3426 3497 3686 3771 3810
Size: 10 178 515 49 71 189 85 39
Cuts at: 3810 4011 4029 4035 4503 4680 4865 4961 5703
Size: 201 18 6 468 177 185 96 742
Cuts at: 5703 5788 6859 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 515 468 399 396 201
189 185 178 177 151 102 96 85
85 85 71 61 52 49 39 37
18 10 6
BspEI T'CCGG_A
Cuts at: 4137 7305 4137
Size: 3168 4474
BspHI T'CATG_A
Cuts at: 5531 7261 5531
Size: 1730 5912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2404 2596 2692 2845 3888 4997 844
Size: 1560 192 96 153 1043 1109 3489
Fragments arranged by size:
3489 1560 1109 1043 192 153 96
BsrBI CCG'CTC
Cuts at: 1394 1823 3459 5529 1394
Size: 429 1636 2070 3507
Fragments arranged by size:
3507 2070 1636 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6137 6311 65
Size: 1183 4889 174 1396
Fragments arranged by size:
4889 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2267 2387 2632 4523 4882 6286 6473
Size: 110 457 120 245 1891 359 1404 187
Cuts at: 6473 1700
Size: 2869
Fragments arranged by size:
2869 1891 1404 457 359 245 187 120
110
BsrGI T'GTAC_A
Cuts at: 96 2970 3964 96
Size: 2874 994 3774
Fragments arranged by size:
3774 2874 994
BssHII G'CGCG_C
Cuts at: 1925 4286 4288 4907 1925
Size: 2361 2 619 4660
Fragments arranged by size:
4660 2361 619 2
BssSI C'ACGA_G
Cuts at: 4375 5702 6714 4375
Size: 1327 1012 5303
Fragments arranged by size:
5303 1327 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2437 4246 4930 794
Size: 1643 1809 684 3506
Fragments arranged by size:
3506 1809 1643 684
BstBI TT'CG_AA
Cuts at: 6446 6446
Size: 7642
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7642
BstXI CCAn_nnnn'nTGG
Cuts at: 3524 3661 4156 3524
Size: 137 495 7010
Fragments arranged by size:
7010 495 137
BstYI r'GATC_y
Cuts at: 1972 2744 4972 5339 5724 5741 7193 7279 7291
Size: 772 2228 367 385 17 1452 86 12
Cuts at: 7291 7637 1972
Size: 346 1977
Fragments arranged by size:
2228 1977 1452 772 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2022 2022
Size: 7642
Bsu36I CC'TnA_GG
Cuts at: 3795 3795
Size: 7642
BtgI C'CryG_G
Cuts at: 513 1065 2004 2593 2676 2707 3315 3368 4179
Size: 552 939 589 83 31 608 53 811
Cuts at: 4179 4221 4578 513
Size: 42 357 3577
Fragments arranged by size:
3577 939 811 608 589 552 357 83
53 42 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2922 4696 487
Size: 683 56 1696 1774 3433
Fragments arranged by size:
3433 1774 1696 683 56
BtsI GCAGTG_nn'
Cuts at: 3067 3163 3208 3277 3400 4244 4469 5158 5952
Size: 96 45 69 123 844 225 689 794
Cuts at: 5952 5972 3067
Size: 20 4737
Fragments arranged by size:
4737 844 794 689 225 123 96 69
45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7642
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7607
DraI TTT'AAA
Cuts at: 4946 4946
Size: 7642
DraIII CAC_nnn'GTG
Cuts at: 2210 2210
Size: 7642
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6649 809
Size: 507 5333 1802
Fragments arranged by size:
5333 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2212 2572 2922
Size: 54 1425 50 275 18 382 360 350
Cuts at: 2922 3015 3054 3585 4218 4740 4884 5089 5978
Size: 93 39 531 633 522 144 205 889
Cuts at: 5978 7607 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 633 531 522 382 360
350 275 205 144 93 54 50 43
39 18
EagI C'GGCC_G
Cuts at: 1537 1812 5089 1537
Size: 275 3277 4090
Fragments arranged by size:
4090 3277 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2526 2656 4553 4782 4922 5572 1353
Size: 1173 130 1897 229 140 650 3423
Fragments arranged by size:
3423 1897 1173 650 229 140 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3100 6229 6603 6749 387
Size: 1064 138 1511 3129 374 146 1280
Fragments arranged by size:
3129 1511 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2236 3769 2236
Size: 1533 6109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2343 2471 2798 2834 2863 3440 3761
Size: 393 711 128 327 36 29 577 321
Cuts at: 3761 3940 4178 4436 4478 4895 4921 5705 6302
Size: 179 238 258 42 417 26 784 597
Cuts at: 6302 7089 7357 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 784 711 597 577 417 393
327 321 268 258 238 179 128 42
36 29 26
EcoNI CCTnn'n_nnAGG
Cuts at: 3919 4277 3919
Size: 358 7284
EcoO109I rG'GnC_Cy
Cuts at: 2065 2670 2671 2809 3832 4072 4591 2065
Size: 605 1 138 1023 240 519 5116
Fragments arranged by size:
5116 1023 605 519 240 138 1
EcoRI G'AATT_C
Cuts at: 4951 5329 4951
Size: 378 7264
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4582 4582
Size: 7642
FspI TGC'GCA
Cuts at: 3533 4604 6148 3533
Size: 1071 1544 5027
Fragments arranged by size:
5027 1544 1071
HaeII r_GCGC'y
Cuts at: 1678 2774 3708 3953 4233 4255 4524 4611 4931
Size: 1096 934 245 280 22 269 87 320
Cuts at: 4931 6789 1678
Size: 1858 2531
Fragments arranged by size:
2531 1858 1096 934 320 280 269 245
87 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2336 2368 2736 2768 6283
Size: 32 135 32 764 32 368 32 3515
Cuts at: 6283 6315 6357 6389 1373
Size: 32 42 32 2626
Fragments arranged by size:
3515 2626 764 368 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3430 3865 4186 4986 5232 669
Size: 988 281 1492 435 321 800 246 3079
Fragments arranged by size:
3079 1492 988 800 435 321 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7642
HpaI GTT'AAC
Cuts at: 5232 5232
Size: 7642
KasI G'GCGC_C
Cuts at: 3949 4229 3949
Size: 280 7362
KpnI G_GTAC'C
Cuts at: 2601 4967 2601
Size: 2366 5276
MfeI C'AATT_G
Cuts at: 5241 5241
Size: 7642
MluI A'CGCG_T
Cuts at: 7518 7518
Size: 7642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2334 3675 6756 6940 1305
Size: 1029 1341 3081 184 2007
Fragments arranged by size:
3081 2007 1341 1029 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2214 2574 3056 3587 4742
Size: 54 1425 343 382 360 482 531 1155
Cuts at: 4742 10
Size: 2910
Fragments arranged by size:
2910 1425 1155 531 482 382 360 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2504 3074 3080 3242 3522 3587 3893
Size: 648 1338 570 6 162 280 65 306
Cuts at: 3893 4004 5600 5959 6118 518
Size: 111 1596 359 159 2042
Fragments arranged by size:
2042 1596 1338 648 570 359 306 280
162 159 111 65 6
MspA1I CmG'CkG
Cuts at: 1606 2111 2141 2434 2491 2698 2797 2968 3144
Size: 505 30 293 57 207 99 171 176
Cuts at: 3144 3228 3958 4223 4389 4411 5058 5735 6883
Size: 84 730 265 166 22 647 677 1148
Cuts at: 6883 7128 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 730 677 647 505 293 265
245 207 176 171 166 99 84 57
30 22
NaeI GCC'GGC
Cuts at: 4525 4884 4525
Size: 359 7283
NarI GG'CG_CC
Cuts at: 3950 4230 3950
Size: 280 7362
NcoI C'CATG_G
Cuts at: 513 1065 2004 2593 4179 4578 513
Size: 552 939 589 1586 399 3577
Fragments arranged by size:
3577 1586 939 589 552 399
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7642
NgoMIV G'CCGG_C
Cuts at: 4523 4882 4523
Size: 359 7283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7642
NotI GC'GGCC_GC
Cuts at: 5089 5089
Size: 7642
NspI r_CATG'y
Cuts at: 1132 1229 3265 4663 5000 6545 1132
Size: 97 2036 1398 337 1545 2229
Fragments arranged by size:
2229 2036 1545 1398 337 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7642
PciI A'CATG_T
Cuts at: 4659 6541 4659
Size: 1882 5760
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2210 3052 3640 4075 4180 1108
Size: 1102 842 588 435 105 4570
Fragments arranged by size:
4570 1102 842 588 435 105
PfoI T'CCnGG_A
Cuts at: 3330 4587 3330
Size: 1257 6385
PmeI GTTT'AAAC
Cuts at: 4946 4946
Size: 7642
PmlI CAC'GTG
Cuts at: 2476 2827 2872 2476
Size: 351 45 7246
Fragments arranged by size:
7246 351 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5711 5743 7224 7256 5711
Size: 32 1481 32 6097
Fragments arranged by size:
6097 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3832 4591 3832
Size: 759 6883
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7642
PsiI TTA'TAA
Cuts at: 5212 5212
Size: 7642
PspOMI G'GGCC_C
Cuts at: 2670 3806 4861 2670
Size: 1136 1055 5451
Fragments arranged by size:
5451 1136 1055
PstI C_TGCA'G
Cuts at: 830 2181 2703 3885 4302 4443 4994 830
Size: 1351 522 1182 417 141 551 3478
Fragments arranged by size:
3478 1351 1182 551 522 417 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7642
PvuII CAG'CTG
Cuts at: 2491 2698 2491
Size: 207 7435
SacI G_AGCT'C
Cuts at: 2238 3771 2238
Size: 1533 6109
SacII CC_GC'GG
Cuts at: 4224 4224
Size: 7642
SalI G'TCGA_C
Cuts at: 1936 3863 4984 1936
Size: 1927 1121 4594
Fragments arranged by size:
4594 1927 1121
SapI GCTCTTCn'nnn_
Cuts at: 2526 2656 4553 2526
Size: 130 1897 5615
Fragments arranged by size:
5615 1897 130
SbfI CC_TGCA'GG
Cuts at: 4994 4994
Size: 7642
ScaI AGT'ACT
Cuts at: 5890 7602 5890
Size: 1712 5930
SfcI C'TryA_G
Cuts at: 826 1045 2030 2177 2699 3881 4298 4439 4570
Size: 219 985 147 522 1182 417 141 131
Cuts at: 4570 4990 6125 6806 6997 826
Size: 420 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 985 681 522 420 417
219 191 147 141 131
SfoI GGC'GCC
Cuts at: 3951 4231 3951
Size: 280 7362
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
SgrAI Cr'CCGG_yG
Cuts at: 1700 2632 1700
Size: 932 6710
SmaI CCC'GGG
Cuts at: 1872 3811 4969 1872
Size: 1939 1158 4545
Fragments arranged by size:
4545 1939 1158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2832 3623 4502 4556 5746 6647
Size: 197 926 889 791 879 54 1190 901
Cuts at: 6647 6909 820
Size: 262 1553
Fragments arranged by size:
1553 1190 926 901 889 879 791 262
197 54
SnaBI TAC'GTA
Cuts at: 493 5433 493
Size: 4940 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7642
SphI G_CATG'C
Cuts at: 1132 5000 1132
Size: 3868 3774
SspI AAT'ATT
Cuts at: 5 52 5566 5
Size: 47 5514 2081
Fragments arranged by size:
5514 2081 47
StuI AGG'CCT
Cuts at: 1517 4373 1517
Size: 2856 4786
StyI C'CwwG_G
Cuts at: 513 1065 2004 2593 3250 4179 4533 4578 4695
Size: 552 939 589 657 929 354 45 117
Cuts at: 4695 5081 513
Size: 386 3074
Fragments arranged by size:
3074 939 929 657 589 552 386 354
117 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2149 2290 2441 2750 3035 4187 4369
Size: 1255 569 141 151 309 285 1152 182
Cuts at: 4369 5669 5686 5839 6024 6469 325
Size: 1300 17 153 185 445 1498
Fragments arranged by size:
1498 1300 1255 1152 569 445 309 285
185 182 153 151 141 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 2874 2970
Size: 274 80 33 51 1443 324 573 96
Cuts at: 2970 3964 4656 4662 5888 7600 96
Size: 994 692 6 1226 1712 138
Fragments arranged by size:
1712 1443 1226 994 692 573 324 274
138 96 80 51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2861 2893 3890 3922 7602 7634 2861
Size: 32 997 32 3680 32 2869
Fragments arranged by size:
3680 2869 997 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2350 1316
Size: 1034 6608
XbaI T'CTAG_A
Cuts at: 4978 4978
Size: 7642
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2533 3637 4000 4717 2533
Size: 1104 363 717 5458
Fragments arranged by size:
5458 1104 717 363
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7642
XmaI C'CCGG_G
Cuts at: 1870 3809 4967 1870
Size: 1939 1158 4545
Fragments arranged by size:
4545 1939 1158
XmnI GAAnn'nnTTC
Cuts at: 3914 5771 3914
Size: 1857 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3868 5450 276
Size: 53 83 186 936 2334 1582 2468
Fragments arranged by size:
2468 2334 1582 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI EcoRV
FseI FspAI NruI NsiI PacI PspXI PsrI RsrII
SanDI SexAI SfiI SrfI SwaI