Restriction map for
pFN21AE1025
(Circular) (Six-base) MAPSORT of: pFN21AE1025.seq Check: 2877 from: 1 to: 7219
pFN21AE1025 7219 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3245 3245
Size: 7219
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5029 278
Size: 53 83 186 936 3493 2468
Fragments arranged by size:
3493 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2293 3471 4562 1937
Size: 356 1178 1091 4594
Fragments arranged by size:
4594 1178 1091 356
Acc65I G'GTAC_C
Cuts at: 4540 4540
Size: 7219
AclI AA'CG_TT
Cuts at: 1563 3266 5346 5719 1563
Size: 1703 2080 373 3063
Fragments arranged by size:
3063 2080 1703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 2151 3987 4413 5282 6666 6934 1632
Size: 488 31 1836 426 869 1384 268 1917
Fragments arranged by size:
1917 1836 1384 869 488 426 268 31
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7022
AflIII A'CryG_T
Cuts at: 1203 2963 6118 7095 1203
Size: 1760 3155 977 1327
Fragments arranged by size:
3155 1760 1327 977
AgeI A'CCGG_T
Cuts at: 6050 6050
Size: 7219
AhdI GACnn_n'nnGTC
Cuts at: 5948 5948
Size: 7219
AleI CACnn'nnGTG
Cuts at: 1166 3894 4324 1166
Size: 2728 430 4061
Fragments arranged by size:
4061 2728 430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7219
AlwNI CAG_nnn'CTG
Cuts at: 2645 2888 3305 4962 6534 2645
Size: 243 417 1657 1572 3330
Fragments arranged by size:
3330 1657 1572 417 243
ApaLI G'TGCA_C
Cuts at: 5276 6432 5276
Size: 1156 6063
ApoI r'AATT_y
Cuts at: 1493 2196 2612 3241 3422 3808 3881 4124 4193
Size: 703 416 629 181 386 73 243 69
Cuts at: 4193 4312 4528 4754 4906 7015 1493
Size: 119 216 226 152 2109 1697
Fragments arranged by size:
2109 1697 703 629 416 386 243 226
216 181 152 119 73 69
AseI AT'TA_AT
Cuts at: 160 1032 5773 160
Size: 872 4741 1606
Fragments arranged by size:
4741 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7219
AvaI C'yCGr_G
Cuts at: 1870 1943 4544 1870
Size: 73 2601 4545
Fragments arranged by size:
4545 2601 73
BamHI G'GATC_C
Cuts at: 3797 4549 4916 3797
Size: 752 367 6100
Fragments arranged by size:
6100 752 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 2778 4540 5995 618
Size: 325 217 627 392 599 1762 1455 1842
Fragments arranged by size:
1842 1762 1455 627 599 392 325 217
BanII G_rGCy'C
Cuts at: 1391 2821 4538 1391
Size: 1430 1717 4072
Fragments arranged by size:
4072 1717 1430
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5410 5444 1903
Size: 34 50 34 3389 34 3678
Fragments arranged by size:
3678 3389 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5111 6321 5111
Size: 1210 6009
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7219
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5830 136
Size: 107 122 71 631 605 4158 1525
Fragments arranged by size:
4158 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2131 2462 7214 2131
Size: 331 4752 2136
Fragments arranged by size:
4752 2136 331
BlpI GC'TnA_GC
Cuts at: 4636 4636
Size: 7219
Bme1580I G_kGCm'C
Cuts at: 1790 2402 5280 6436 1790
Size: 612 2878 1156 2573
Fragments arranged by size:
2878 2573 1156 612
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7219
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3107 4950 5908
Size: 416 53 83 399 24 1690 1843 958
Cuts at: 5908 442
Size: 1753
Fragments arranged by size:
1843 1753 1690 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2652 2898 3088 3270 3651 5879 1239
Size: 1413 246 190 182 381 2228 2579
Fragments arranged by size:
2579 2228 1413 381 246 190 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4102 5344 6209 6507 4102
Size: 1242 865 298 4814
Fragments arranged by size:
4814 1242 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5882 882
Size: 617 4383 2219
Fragments arranged by size:
4383 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5010 493
Size: 711 3806 2702
Fragments arranged by size:
3806 2702 711
BsaBI GATnn'nnATC
Cuts at: 2130 2501 7213 2130
Size: 371 4712 2136
Fragments arranged by size:
4712 2136 371
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5026 5408 6043 275
Size: 53 83 186 936 3493 382 635 1451
Fragments arranged by size:
3493 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5652 6050 6324 6471 6882 5652
Size: 398 274 147 411 5989
Fragments arranged by size:
5989 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6994
Fragments arranged by size:
6994 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7219
BseYI C'CCAG_C
Cuts at: 6422 6918 6422
Size: 496 6723
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6646
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4669 5430 6458 1540
Size: 275 186 2668 761 1028 2301
Fragments arranged by size:
2668 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3118 5280 5365 6436 3118
Size: 2162 85 1071 3901
Fragments arranged by size:
3901 2162 1071 85
BsmI GAATG_Cn'
Cuts at: 2419 3171 4730 4823 2419
Size: 752 1559 93 4815
Fragments arranged by size:
4815 1559 752 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4061 4061
Size: 7219
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2402 2821 3118 4538 5280 5365 6436
Size: 399 612 419 297 1420 742 85 1071
Cuts at: 6436 1391
Size: 2174
Fragments arranged by size:
2174 1420 1071 742 612 419 399 297
85
BspEI T'CCGG_A
Cuts at: 6882 6882
Size: 7219
BspHI T'CATG_A
Cuts at: 5108 6838 5108
Size: 1730 5489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3245 4574 844
Size: 2401 1329 3489
Fragments arranged by size:
3489 2401 1329
BsrBI CCG'CTC
Cuts at: 1394 1823 5106 1394
Size: 429 3283 3507
Fragments arranged by size:
3507 3283 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3144 3165 3406 3795 5714 5888 65
Size: 1183 1896 21 241 389 1919 174 1396
Fragments arranged by size:
1919 1896 1396 1183 389 241 174 21
BsrFI r'CCGG_y
Cuts at: 1700 1810 5863 6050 1700
Size: 110 4053 187 2869
Fragments arranged by size:
4053 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3706 96
Size: 3610 3609
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7219
BssSI C'ACGA_G
Cuts at: 5279 6291 5279
Size: 1012 6207
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7219
BstBI TT'CG_AA
Cuts at: 6023 6023
Size: 7219
BstEII G'GTnAC_C
Cuts at: 1184 4342 1184
Size: 3158 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 3619 3790 3619
Size: 171 7048
BstYI r'GATC_y
Cuts at: 1972 2131 2462 2641 3797 4549 4916 5301 5318
Size: 159 331 179 1156 752 367 385 17
Cuts at: 5318 6770 6856 6868 7214 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1156 752 385 367 346 331
179 159 86 17 12
BstZ17I GTA'TAC
Cuts at: 2294 2294
Size: 7219
Bsu36I CC'TnA_GG
Cuts at: 4287 4287
Size: 7219
BtgI C'CryG_G
Cuts at: 513 1065 3618 3666 513
Size: 552 2553 48 4066
Fragments arranged by size:
4066 2553 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4458 487
Size: 683 56 3232 3248
Fragments arranged by size:
3248 3232 683 56
BtsI GCAGTG_nn'
Cuts at: 3306 3928 4735 5529 5549 3306
Size: 622 807 794 20 4976
Fragments arranged by size:
4976 807 794 622 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7219
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7184
DraI TTT'AAA
Cuts at: 4523 4523
Size: 7219
DraIII CAC_nnn'GTG
Cuts at: 2915 3990 2915
Size: 1075 6144
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6226 809
Size: 507 4910 1802
Fragments arranged by size:
4910 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3246 3609 4666
Size: 54 1425 50 275 18 1416 363 1057
Cuts at: 4666 5555 7184 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1416 1057 889 363 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4666 1537
Size: 275 2854 4090
Fragments arranged by size:
4090 2854 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2350 5149 1353
Size: 997 2799 3423
Fragments arranged by size:
3423 2799 997
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5806 6180 6326 387
Size: 1064 138 4217 374 146 1280
Fragments arranged by size:
4217 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2151 2652 2898 3088 3270 3651
Size: 393 488 31 501 246 190 182 381
Cuts at: 3651 3987 4413 5282 5879 6666 6934 1239
Size: 336 426 869 597 787 268 1524
Fragments arranged by size:
1524 869 787 597 501 488 426 393
381 336 268 246 190 182 31
EcoRI G'AATT_C
Cuts at: 3808 4528 4906 3808
Size: 720 378 6121
Fragments arranged by size:
6121 720 378
FspI TGC'GCA
Cuts at: 5725 5725
Size: 7219
HaeII r_GCGC'y
Cuts at: 1678 6366 1678
Size: 4688 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 2092 2123 2124
Size: 32 135 32 485 32 3 31 1
Cuts at: 2124 2155 2306 2338 2440 2472 2938 2970 3187
Size: 31 151 32 102 32 466 32 217
Cuts at: 3187 3219 3464 3496 3851 3883 4121 4153 4160
Size: 32 245 32 355 32 238 32 7
Cuts at: 4160 4192 5860 5892 5934 5966 1373
Size: 32 1668 32 42 32 2626
Fragments arranged by size:
2626 1668 485 466 355 245 238 217
151 135 102 42 32 32 32 32
32 32 32 32 32 32 32 32
32 31 31 7 3 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4276 4563 4809 669
Size: 988 281 2338 287 246 3079
Fragments arranged by size:
3079 2338 988 287 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7219
HpaI GTT'AAC
Cuts at: 4276 4809 4276
Size: 533 6686
KpnI G_GTAC'C
Cuts at: 4544 4544
Size: 7219
MfeI C'AATT_G
Cuts at: 4818 4818
Size: 7219
MluI A'CGCG_T
Cuts at: 7095 7095
Size: 7219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3103 3669 6333 6517 1305
Size: 1798 566 2664 184 2007
Fragments arranged by size:
2664 2007 1798 566 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3248 10
Size: 54 1425 343 1416 3981
Fragments arranged by size:
3981 1425 1416 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2698 3040 3176 3894 4324 4337 5177
Size: 648 1532 342 136 718 430 13 840
Cuts at: 5177 5536 5695 518
Size: 359 159 2042
Fragments arranged by size:
2042 1532 840 718 648 430 359 342
159 136 13
MspA1I CmG'CkG
Cuts at: 1606 2709 2888 3467 4635 5312 6460 6705 1606
Size: 1103 179 579 1168 677 1148 245 2120
Fragments arranged by size:
2120 1168 1148 1103 677 579 245 179
NcoI C'CATG_G
Cuts at: 513 1065 3618 3666 513
Size: 552 2553 48 4066
Fragments arranged by size:
4066 2553 552 48
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7219
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7219
NotI GC'GGCC_GC
Cuts at: 4666 4666
Size: 7219
NspI r_CATG'y
Cuts at: 1132 1229 2967 4577 6122 1132
Size: 97 1738 1610 1545 2229
Fragments arranged by size:
2229 1738 1610 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7219
PciI A'CATG_T
Cuts at: 2963 6118 2963
Size: 3155 4064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2675 3990 1108
Size: 1567 1315 4337
Fragments arranged by size:
4337 1567 1315
PmeI GTTT'AAAC
Cuts at: 4523 4523
Size: 7219
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5288 5320 6801 6833 5288
Size: 32 1481 32 5674
Fragments arranged by size:
5674 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7219
PsiI TTA'TAA
Cuts at: 4789 4789
Size: 7219
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4225 4257 4225
Size: 32 7187
PstI C_TGCA'G
Cuts at: 830 3301 4571 830
Size: 2471 1270 3478
Fragments arranged by size:
3478 2471 1270
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7219
PvuII CAG'CTG
Cuts at: 2709 2888 3467 2709
Size: 179 579 6461
Fragments arranged by size:
6461 579 179
SalI G'TCGA_C
Cuts at: 1936 4561 1936
Size: 2625 4594
SbfI CC_TGCA'GG
Cuts at: 4571 4571
Size: 7219
ScaI AGT'ACT
Cuts at: 5467 7179 5467
Size: 1712 5507
SfcI C'TryA_G
Cuts at: 826 1045 3297 3306 3468 4161 4567 5702 6383
Size: 219 2252 9 162 693 406 1135 681
Cuts at: 6383 6574 826
Size: 191 1471
Fragments arranged by size:
2252 1471 1135 693 681 406 219 191
162 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7219
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7219
SmaI CCC'GGG
Cuts at: 1872 4546 1872
Size: 2674 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4117 5323 6224 6486 820
Size: 197 926 2174 1206 901 262 1553
Fragments arranged by size:
2174 1553 1206 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5010 493
Size: 4517 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7219
SphI G_CATG'C
Cuts at: 1132 4577 1132
Size: 3445 3774
SspI AAT'ATT
Cuts at: 5 52 2489 2524 3087 5143 5
Size: 47 2437 35 563 2056 2081
Fragments arranged by size:
2437 2081 2056 563 47 35
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7219
StyI C'CwwG_G
Cuts at: 513 1065 3618 3666 4658 513
Size: 552 2553 48 992 3074
Fragments arranged by size:
3074 2553 992 552 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2197 5246 5263 5416 5601 6046 325
Size: 1255 617 3049 17 153 185 445 1498
Fragments arranged by size:
3049 1498 1255 617 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2309 2540 3044
Size: 274 80 33 51 1443 332 231 504
Cuts at: 3044 3694 3706 5465 7177 96
Size: 650 12 1759 1712 138
Fragments arranged by size:
1759 1712 1443 650 504 332 274 231
138 80 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2670 2702 7179 7211 2670
Size: 32 4477 32 2678
Fragments arranged by size:
4477 2678 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2069 1316
Size: 753 6466
XbaI T'CTAG_A
Cuts at: 4555 4555
Size: 7219
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7219
XmaI C'CCGG_G
Cuts at: 1870 4544 1870
Size: 2674 4545
XmnI GAAnn'nnTTC
Cuts at: 2197 5348 2197
Size: 3151 4068
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5027 276
Size: 53 83 186 936 3493 2468
Fragments arranged by size:
3493 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI