Restriction map for
pFN21AE1024
(Circular) (Six-base) MAPSORT of: pFN21AE1024.seq Check: 9356 from: 1 to: 7129
pFN21AE1024 7129 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3537 3537
Size: 7129
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4939 278
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4472 1937
Size: 2535 4594
Acc65I G'GTAC_C
Cuts at: 4450 4450
Size: 7129
AclI AA'CG_TT
Cuts at: 1563 5256 5629 1563
Size: 3693 373 3063
Fragments arranged by size:
3693 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3320 5192 6576 6844 1632
Size: 1688 1872 1384 268 1917
Fragments arranged by size:
1917 1872 1688 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6932
AflIII A'CryG_T
Cuts at: 1203 3015 3630 6028 7005 1203
Size: 1812 615 2398 977 1327
Fragments arranged by size:
2398 1812 1327 977 615
AgeI A'CCGG_T
Cuts at: 5960 5960
Size: 7129
AhdI GACnn_n'nnGTC
Cuts at: 5858 5858
Size: 7129
AleI CACnn'nnGTG
Cuts at: 1166 2159 2270 1166
Size: 993 111 6025
Fragments arranged by size:
6025 993 111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2613 3399 1184
Size: 1429 786 4914
Fragments arranged by size:
4914 1429 786
AlwNI CAG_nnn'CTG
Cuts at: 2357 2651 3027 3121 3286 4085 4872 6444 2357
Size: 294 376 94 165 799 787 1572 3042
Fragments arranged by size:
3042 1572 799 787 376 294 165 94
ApaI G_GGCC'C
Cuts at: 2100 3968 4033 4368 2100
Size: 1868 65 335 4861
Fragments arranged by size:
4861 1868 335 65
ApaLI G'TGCA_C
Cuts at: 5186 6342 5186
Size: 1156 5973
ApoI r'AATT_y
Cuts at: 1493 4438 4664 4816 6925 1493
Size: 2945 226 152 2109 1697
Fragments arranged by size:
2945 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5683 160
Size: 872 4651 1606
Fragments arranged by size:
4651 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
AvaI C'yCGr_G
Cuts at: 1870 1943 2079 2088 2168 2194 2781 2950 3261
Size: 73 136 9 80 26 587 169 311
Cuts at: 3261 3921 4014 4454 1870
Size: 660 93 440 4545
Fragments arranged by size:
4545 660 587 440 311 169 136 93
80 73 26 9
BamHI G'GATC_C
Cuts at: 3084 4459 4826 3084
Size: 1375 367 5387
Fragments arranged by size:
5387 1375 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2051 2492 3402 4450
Size: 325 217 627 233 31 441 910 1048
Cuts at: 4450 5905 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1048 910 627 441 325 233
217 31
BanII G_rGCy'C
Cuts at: 1391 2100 2346 2380 2747 2870 2879 2969 3003
Size: 709 246 34 367 123 9 90 34
Cuts at: 3003 3184 3701 3968 4033 4368 4448 1391
Size: 181 517 267 65 335 80 4072
Fragments arranged by size:
4072 709 517 367 335 267 246 181
123 90 80 65 34 34 9
BbeI G_GCGC'C
Cuts at: 2024 2055 2024
Size: 31 7098
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2411 3789 928
Size: 971 512 1378 4268
Fragments arranged by size:
4268 1378 971 512
BbvCI CC'TCA_GC
Cuts at: 3983 4098 4399 3983
Size: 115 301 6713
Fragments arranged by size:
6713 301 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2195 2229 3236 3270 5320 5354 1903
Size: 34 258 34 1007 34 2050 34 3678
Fragments arranged by size:
3678 2050 1007 258 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5021 6231 5021
Size: 1210 5919
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7129
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2060 2144
Size: 107 122 71 631 605 377 11 84
Cuts at: 2144 2390 2785 3356 3904 3993 5740 136
Size: 246 395 571 548 89 1747 1525
Fragments arranged by size:
1747 1525 631 605 571 548 395 377
246 122 107 89 84 71 11
BglII A'GATC_T
Cuts at: 3414 3480 7124 3414
Size: 66 3644 3419
Fragments arranged by size:
3644 3419 66
BlpI GC'TnA_GC
Cuts at: 4546 4546
Size: 7129
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2100 2497 3968 4033 4368 5190 6346
Size: 228 82 397 1471 65 335 822 1156
Cuts at: 6346 1790
Size: 2573
Fragments arranged by size:
2573 1471 1156 822 397 335 228 82
65
BmgBI CAC'GTC
Cuts at: 1318 2751 2983 1318
Size: 1433 232 5464
Fragments arranged by size:
5464 1433 232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3037 4860 5818
Size: 416 53 83 399 24 1620 1823 958
Cuts at: 5818 442
Size: 1753
Fragments arranged by size:
1823 1753 1620 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 2306 2379 3164 3476 3958 4044 5789
Size: 870 197 73 785 312 482 86 1745
Cuts at: 5789 1239
Size: 2579
Fragments arranged by size:
2579 1745 870 785 482 312 197 86
73
Bpu10I CC'TnA_GC
Cuts at: 3983 4098 4399 3983
Size: 115 301 6713
Fragments arranged by size:
6713 301 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2718 3842 3983 4311 5254 6119 6417 2718
Size: 1124 141 328 943 865 298 3430
Fragments arranged by size:
3430 1124 943 865 328 298 141
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2201 3731 4157 5792 882
Size: 617 702 1530 426 1635 2219
Fragments arranged by size:
2219 1635 1530 702 617 426
BsaAI yAC'GTr
Cuts at: 493 1204 4920 493
Size: 711 3716 2702
Fragments arranged by size:
3716 2702 711
BsaBI GATnn'nnATC
Cuts at: 7123 7123
Size: 7129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 2052 3608 4936
Size: 53 83 186 936 488 31 1556 1328
Cuts at: 4936 5318 5953 275
Size: 382 635 1451
Fragments arranged by size:
1556 1451 1328 936 635 488 382 186
83 53 31
BsaWI w'CCGG_w
Cuts at: 2683 2713 5562 5960 6234 6381 6792 2683
Size: 30 2849 398 274 147 411 3020
Fragments arranged by size:
3020 2849 411 398 274 147 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2200 2230 2695 2725 2729
Size: 30 165 30 797 30 465 30 4
Cuts at: 2729 2759 2801 2831 3875 3905 1178
Size: 30 42 30 1044 30 4402
Fragments arranged by size:
4402 1044 797 465 165 42 30 30
30 30 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 3082 3182 3950 3961 3971 1186
Size: 1240 656 100 768 11 10 4344
Fragments arranged by size:
4344 1240 768 656 100 11 10
BseYI C'CCAG_C
Cuts at: 2230 2242 2707 3694 3765 3852 4067 6332 6828
Size: 12 465 987 71 87 215 2265 496
Cuts at: 6828 2230
Size: 2531
Fragments arranged by size:
2531 2265 987 496 465 215 87 71
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2541 3209 3431 3468 1163
Size: 573 805 668 222 37 4824
Fragments arranged by size:
4824 805 668 573 222 37
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2205 4579 5340 6368 1540
Size: 275 186 204 2374 761 1028 2301
Fragments arranged by size:
2374 2301 1028 761 275 204 186
BsiHKAI G_wGCw'C
Cuts at: 2870 2969 3003 3558 5190 5275 6346 2870
Size: 99 34 555 1632 85 1071 3653
Fragments arranged by size:
3653 1632 1071 555 99 85 34
BsmI GAATG_Cn'
Cuts at: 3850 4640 4733 3850
Size: 790 93 6246
Fragments arranged by size:
6246 790 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2003
Size: 7129
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2100 2346 2380 2497 2747 2870
Size: 399 228 82 246 34 117 250 123
Cuts at: 2870 2879 2969 3003 3184 3558 3701 3968 4033
Size: 9 90 34 181 374 143 267 65
Cuts at: 4033 4368 4448 5190 5275 6346 1391
Size: 335 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 374 335 267 250
246 228 181 143 123 117 90 85
82 80 65 34 34 9
BspEI T'CCGG_A
Cuts at: 2713 6792 2713
Size: 4079 3050
BspHI T'CATG_A
Cuts at: 5018 6748 5018
Size: 1730 5399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3231 3537 3768 4484 844
Size: 2387 306 231 716 3489
Fragments arranged by size:
3489 2387 716 306 231
BsrBI CCG'CTC
Cuts at: 1394 1823 3838 4186 4207 4228 4240 4303 5016
Size: 429 2015 348 21 21 12 63 713
Cuts at: 5016 1394
Size: 3507
Fragments arranged by size:
3507 2015 713 429 348 63 21 21
12
BsrDI GCAATG_nn'
Cuts at: 65 1248 5624 5798 65
Size: 1183 4376 174 1396
Fragments arranged by size:
4376 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3318 3814 3942 5773 5960 1700
Size: 110 1508 496 128 1831 187 2869
Fragments arranged by size:
2869 1831 1508 496 187 128 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7129
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7129
BssSI C'ACGA_G
Cuts at: 3245 3932 5189 6201 3245
Size: 687 1257 1012 4173
Fragments arranged by size:
4173 1257 1012 687
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2564 3775 794
Size: 1770 1211 4148
Fragments arranged by size:
4148 1770 1211
BstBI TT'CG_AA
Cuts at: 5933 5933
Size: 7129
BstEII G'GTnAC_C
Cuts at: 1184 3802 1184
Size: 2618 4511
BstXI CCAn_nnnn'nTGG
Cuts at: 2977 3202 3469 4385 2977
Size: 225 267 916 5721
Fragments arranged by size:
5721 916 267 225
BstYI r'GATC_y
Cuts at: 1972 3084 3414 3480 4459 4826 5211 5228 6680
Size: 1112 330 66 979 367 385 17 1452
Cuts at: 6680 6766 6778 7124 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1112 979 385 367 346 330
86 66 17 12
Bsu36I CC'TnA_GG
Cuts at: 2545 2644 2545
Size: 99 7030
BtgI C'CryG_G
Cuts at: 513 1065 2004 2058 2346 2871 2976 3354 3741
Size: 552 939 54 288 525 105 378 387
Cuts at: 3741 513
Size: 3901
Fragments arranged by size:
3901 939 552 525 387 378 288 105
54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3506 487
Size: 683 56 2280 4110
Fragments arranged by size:
4110 2280 683 56
BtsI GCAGTG_nn'
Cuts at: 2358 3776 4092 4645 5439 5459 2358
Size: 1418 316 553 794 20 4028
Fragments arranged by size:
4028 1418 794 553 316 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7129
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7094
DraI TTT'AAA
Cuts at: 4433 4433
Size: 7129
DraIII CAC_nnn'GTG
Cuts at: 2564 2564
Size: 7129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2845 6136 809
Size: 507 1529 3291 1802
Fragments arranged by size:
3291 1802 1529 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2202 2382
Size: 54 1425 50 275 18 306 66 180
Cuts at: 2382 4576 5465 7094 8
Size: 2194 889 1629 43
Fragments arranged by size:
2194 1629 1425 889 306 275 180 66
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2202 4576 1537
Size: 275 390 2374 4090
Fragments arranged by size:
4090 2374 390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5059 1353
Size: 3706 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2870 5716 6090 6236 387
Size: 1064 138 1281 2846 374 146 1280
Fragments arranged by size:
2846 1281 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2868 2967 3001 2868
Size: 99 34 6996
Fragments arranged by size:
6996 99 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2306 2379 3164 3320 3476 3958
Size: 393 477 197 73 785 156 156 482
Cuts at: 3958 4044 5192 5789 6576 6844 1239
Size: 86 1148 597 787 268 1524
Fragments arranged by size:
1524 1148 787 785 597 482 477 393
268 197 156 156 86 73
EcoNI CCTnn'n_nnAGG
Cuts at: 2655 4077 2655
Size: 1422 5707
EcoO109I rG'GnC_Cy
Cuts at: 2120 2191 2445 2457 2469 2648 2660 2762 2791
Size: 71 254 12 12 179 12 102 29
Cuts at: 2791 2838 3190 3220 3283 3349 3918 3965 4029
Size: 47 352 30 63 66 569 47 64
Cuts at: 4029 4030 4082 4365 4373 4395 2120
Size: 1 52 283 8 22 4854
Fragments arranged by size:
4854 569 352 283 254 179 102 71
66 64 63 52 47 47 30 29
22 12 12 12 8 1
EcoRI G'AATT_C
Cuts at: 4438 4816 4438
Size: 378 6751
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2944 3676 2944
Size: 732 6397
FspI TGC'GCA
Cuts at: 2909 5635 2909
Size: 2726 4403
HaeII r_GCGC'y
Cuts at: 1678 2024 2055 3943 6276 1678
Size: 346 31 1888 2333 2531
Fragments arranged by size:
2531 2333 1888 346 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2200 2232 2627 2659 2695
Size: 32 135 32 628 32 395 32 36
Cuts at: 2695 2727 2729 2754 2761 2786 2852 2884 4382
Size: 32 2 25 7 25 66 32 1498
Cuts at: 4382 4414 5770 5802 5844 5876 1373
Size: 32 1356 32 42 32 2626
Fragments arranged by size:
2626 1498 1356 628 395 135 66 42
36 32 32 32 32 32 32 32
32 32 25 25 7 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4473 4719 669
Size: 988 281 2535 246 3079
Fragments arranged by size:
3079 2535 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4337 748
Size: 3589 3540
HpaI GTT'AAC
Cuts at: 4719 4719
Size: 7129
KasI G'GCGC_C
Cuts at: 2020 2051 2020
Size: 31 7098
KpnI G_GTAC'C
Cuts at: 4454 4454
Size: 7129
MfeI C'AATT_G
Cuts at: 4728 4728
Size: 7129
MluI A'CGCG_T
Cuts at: 7005 7005
Size: 7129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6243 6427 1305
Size: 4938 184 2007
Fragments arranged by size:
4938 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5307
Fragments arranged by size:
5307 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 2270 2975 3377 4383 5087 5446
Size: 648 993 111 705 402 1006 704 359
Cuts at: 5446 5605 518
Size: 159 2042
Fragments arranged by size:
2042 1006 993 705 704 648 402 359
159 111
MspA1I CmG'CkG
Cuts at: 1606 2060 2348 2995 3469 3514 3751 4022 4545
Size: 454 288 647 474 45 237 271 523
Cuts at: 4545 5222 6370 6615 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 647 523 474 454 288
271 245 237 45
NaeI GCC'GGC
Cuts at: 3944 3944
Size: 7129
NarI GG'CG_CC
Cuts at: 2021 2052 2021
Size: 31 7098
NcoI C'CATG_G
Cuts at: 513 1065 2004 2871 3741 513
Size: 552 939 867 870 3901
Fragments arranged by size:
3901 939 870 867 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7129
NgoMIV G'CCGG_C
Cuts at: 3942 3942
Size: 7129
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7129
NotI GC'GGCC_GC
Cuts at: 4576 4576
Size: 7129
NspI r_CATG'y
Cuts at: 1132 1229 3019 3382 3634 3733 3778 4487 6032
Size: 97 1790 363 252 99 45 709 1545
Cuts at: 6032 1132
Size: 2229
Fragments arranged by size:
2229 1790 1545 709 363 252 99 97
45
PasI CC'CwG_GG
Cuts at: 1348 3603 3961 4079 1348
Size: 2255 358 118 4398
Fragments arranged by size:
4398 2255 358 118
PciI A'CATG_T
Cuts at: 3015 3630 6028 3015
Size: 615 2398 4116
Fragments arranged by size:
4116 2398 615
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3111 3694 1108
Size: 2003 583 4543
Fragments arranged by size:
4543 2003 583
PfoI T'CCnGG_A
Cuts at: 2116 2116
Size: 7129
PmeI GTTT'AAAC
Cuts at: 4433 4433
Size: 7129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5198 5230 6711 6743 5198
Size: 32 1481 32 5584
Fragments arranged by size:
5584 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2120 2191 2445 2457 2469 2762 2838 3190 3220
Size: 71 254 12 12 293 76 352 30
Cuts at: 3220 3283 3918 2120
Size: 63 635 5331
Fragments arranged by size:
5331 635 352 293 254 76 71 63
30 12 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7129
PsiI TTA'TAA
Cuts at: 4699 4699
Size: 7129
PspOMI G'GGCC_C
Cuts at: 2096 3964 4029 4364 2096
Size: 1868 65 335 4861
Fragments arranged by size:
4861 1868 335 65
PspXI vC'TCGA_Gb
Cuts at: 2781 3261 2781
Size: 480 6649
PstI C_TGCA'G
Cuts at: 830 3891 4481 830
Size: 3061 590 3478
Fragments arranged by size:
3478 3061 590
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7129
PvuII CAG'CTG
Cuts at: 3469 3751 4022 3469
Size: 282 271 6576
Fragments arranged by size:
6576 282 271
SacI G_AGCT'C
Cuts at: 2870 2969 3003 2870
Size: 99 34 6996
Fragments arranged by size:
6996 99 34
SacII CC_GC'GG
Cuts at: 2061 2349 2061
Size: 288 6841
SalI G'TCGA_C
Cuts at: 1936 4471 1936
Size: 2535 4594
SanDI GG'GwC_CC
Cuts at: 2191 2445 2457 3190 3918 2191
Size: 254 12 733 728 5402
Fragments arranged by size:
5402 733 728 254 12
SbfI CC_TGCA'GG
Cuts at: 4481 4481
Size: 7129
ScaI AGT'ACT
Cuts at: 2948 5377 7089 2948
Size: 2429 1712 2988
Fragments arranged by size:
2988 2429 1712
SfcI C'TryA_G
Cuts at: 826 1045 3442 3887 4477 5612 6293 6484 826
Size: 219 2397 445 590 1135 681 191 1471
Fragments arranged by size:
2397 1471 1135 681 590 445 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2144 2144
Size: 7129
SfoI GGC'GCC
Cuts at: 2022 2053 2022
Size: 31 7098
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
SgrAI Cr'CCGG_yG
Cuts at: 1700 3942 1700
Size: 2242 4887
SmaI CCC'GGG
Cuts at: 1872 2081 2090 2170 2196 3923 4016 4456 1872
Size: 209 9 80 26 1727 93 440 4545
Fragments arranged by size:
4545 1727 440 209 93 80 26 9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2733 2781 3261 3857 3998 4326
Size: 197 926 790 48 480 596 141 328
Cuts at: 4326 5233 6134 6396 820
Size: 907 901 262 1553
Fragments arranged by size:
1553 926 907 901 790 596 480 328
262 197 141 48
SnaBI TAC'GTA
Cuts at: 493 4920 493
Size: 4427 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7129
SphI G_CATG'C
Cuts at: 1132 3778 4487 1132
Size: 2646 709 3774
Fragments arranged by size:
3774 2646 709
SspI AAT'ATT
Cuts at: 5 52 5053 5
Size: 47 5001 2081
Fragments arranged by size:
5001 2081 47
StuI AGG'CCT
Cuts at: 1517 2291 3305 1517
Size: 774 1014 5341
Fragments arranged by size:
5341 1014 774
StyI C'CwwG_G
Cuts at: 513 1065 2004 2841 2850 2871 2932 3724 3741
Size: 552 939 837 9 21 61 792 17
Cuts at: 3741 4146 4568 513
Size: 405 422 3074
Fragments arranged by size:
3074 939 837 792 552 422 405 61
21 17 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4141 5156 5173 5326 5511 5956 325
Size: 1255 2561 1015 17 153 185 445 1498
Fragments arranged by size:
2561 1498 1255 1015 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2946 5375 7087
Size: 274 80 33 51 1443 969 2429 1712
Cuts at: 7087 96
Size: 138
Fragments arranged by size:
2429 1712 1443 969 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2112 2144 2523 2555 3036 3068 3182 3214 7089
Size: 32 379 32 481 32 114 32 3875
Cuts at: 7089 7121 2112
Size: 32 2120
Fragments arranged by size:
3875 2120 481 379 114 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7129
XbaI T'CTAG_A
Cuts at: 4465 4465
Size: 7129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2755 2755
Size: 7129
XhoI C'TCGA_G
Cuts at: 1943 2781 3261 1943
Size: 838 480 5811
Fragments arranged by size:
5811 838 480
XmaI C'CCGG_G
Cuts at: 1870 2079 2088 2168 2194 3921 4014 4454 1870
Size: 209 9 80 26 1727 93 440 4545
Fragments arranged by size:
4545 1727 440 209 93 80 26 9
XmnI GAAnn'nnTTC
Cuts at: 5258 5258
Size: 7129
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4937 276
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI
PsrI RsrII SapI SexAI SrfI SwaI