Restriction map for
pFN21AE1019
(Circular) (Six-base) MAPSORT of: pFN21AE1019.seq Check: 7331 from: 1 to: 6694
pFN21AE1019 6694 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2517 4504 278
Size: 53 83 186 936 981 1987 2468
Fragments arranged by size:
2468 1987 981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4037 1937
Size: 2100 4594
Acc65I G'GTAC_C
Cuts at: 2819 4015 2819
Size: 1196 5498
AclI AA'CG_TT
Cuts at: 1563 4821 5194 1563
Size: 3258 373 3063
Fragments arranged by size:
3258 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3233 3386 3680 3813 4757 6141 6409 1632
Size: 1601 153 294 133 944 1384 268 1917
Fragments arranged by size:
1917 1601 1384 944 294 268 153 133
AfeI AGC'GCT
Cuts at: 2988 2988
Size: 6694
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6497
AflIII A'CryG_T
Cuts at: 1203 5593 6570 1203
Size: 4390 977 1327
Fragments arranged by size:
4390 1327 977
AgeI A'CCGG_T
Cuts at: 5525 5525
Size: 6694
AhdI GACnn_n'nnGTC
Cuts at: 5423 5423
Size: 6694
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6694
AlwNI CAG_nnn'CTG
Cuts at: 3244 4437 6009 3244
Size: 1193 1572 3929
Fragments arranged by size:
3929 1572 1193
ApaLI G'TGCA_C
Cuts at: 4751 5907 4751
Size: 1156 5538
ApoI r'AATT_y
Cuts at: 1493 3380 3506 4003 4229 4381 6490 1493
Size: 1887 126 497 226 152 2109 1697
Fragments arranged by size:
2109 1887 1697 497 226 152 126
AseI AT'TA_AT
Cuts at: 160 1032 5248 160
Size: 872 4216 1606
Fragments arranged by size:
4216 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
AvaI C'yCGr_G
Cuts at: 1870 1943 2113 4019 1870
Size: 73 170 1906 4545
Fragments arranged by size:
4545 1906 170 73
BamHI G'GATC_C
Cuts at: 3099 4024 4391 3099
Size: 925 367 5402
Fragments arranged by size:
5402 925 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2819 4015 5470 618
Size: 325 217 627 1032 1196 1455 1842
Fragments arranged by size:
1842 1455 1196 1032 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2744 4013 1391
Size: 1353 1269 4072
Fragments arranged by size:
4072 1353 1269
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2238 928
Size: 971 339 5384
Fragments arranged by size:
5384 971 339
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4885 4919 1903
Size: 34 2948 34 3678
Fragments arranged by size:
3678 2948 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4586 5796 4586
Size: 1210 5484
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6694
BfrBI ATG'CAT
Cuts at: 3476 3476
Size: 6694
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3275 5305 136
Size: 107 122 71 631 605 1603 2030 1525
Fragments arranged by size:
2030 1603 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6689 6689
Size: 6694
BlpI GC'TnA_GC
Cuts at: 4111 4111
Size: 6694
Bme1580I G_kGCm'C
Cuts at: 1790 4755 5911 1790
Size: 2965 1156 2573
Fragments arranged by size:
2965 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6694
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4425 5383 442
Size: 416 53 83 399 24 3008 958 1753
Fragments arranged by size:
3008 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6694
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 6694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2002 2406 2544 2720 3126 5354 1239
Size: 763 404 138 176 406 2228 2579
Fragments arranged by size:
2579 2228 763 406 404 176 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2500 3845 4819 5684 5982 2500
Size: 1345 974 865 298 3212
Fragments arranged by size:
3212 1345 974 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2473 5357 882
Size: 617 974 2884 2219
Fragments arranged by size:
2884 2219 974 617
BsaAI yAC'GTr
Cuts at: 493 1204 4485 493
Size: 711 3281 2702
Fragments arranged by size:
3281 2702 711
BsaBI GATnn'nnATC
Cuts at: 3945 6688 3945
Size: 2743 3951
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2514 2672 4501 4883
Size: 53 83 186 936 981 158 1829 382
Cuts at: 4883 5518 275
Size: 635 1451
Fragments arranged by size:
1829 1451 981 936 635 382 186 158
83 53
BsaWI w'CCGG_w
Cuts at: 5127 5525 5799 5946 6357 5127
Size: 398 274 147 411 5464
Fragments arranged by size:
5464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6469
Fragments arranged by size:
6469 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2681 1186
Size: 1495 5199
BseYI C'CCAG_C
Cuts at: 2054 3237 3521 5897 6393 2054
Size: 1183 284 2376 496 2355
Fragments arranged by size:
2376 2355 1183 496 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6121
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4144 4905 5933 1540
Size: 275 186 2143 761 1028 2301
Fragments arranged by size:
2301 2143 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2744 4755 4840 5911 2744
Size: 2011 85 1071 3527
Fragments arranged by size:
3527 2011 1071 85
BsmI GAATG_Cn'
Cuts at: 3027 4205 4298 3027
Size: 1178 93 5423
Fragments arranged by size:
5423 1178 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2456 2663 2456
Size: 207 6487
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2744 4013 4755 4840 5911 1391
Size: 399 954 1269 742 85 1071 2174
Fragments arranged by size:
2174 1269 1071 954 742 399 85
BspEI T'CCGG_A
Cuts at: 6357 6357
Size: 6694
BspHI T'CATG_A
Cuts at: 3429 4583 6313 3429
Size: 1154 1730 3810
Fragments arranged by size:
3810 1730 1154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4049 844
Size: 3205 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4581 1394
Size: 429 2758 3507
Fragments arranged by size:
3507 2758 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3447 5189 5363 65
Size: 1183 2199 1742 174 1396
Fragments arranged by size:
2199 1742 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2354 5338 5525 1700
Size: 110 544 2984 187 2869
Fragments arranged by size:
2984 2869 544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6694
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6694
BssSI C'ACGA_G
Cuts at: 4754 5766 4754
Size: 1012 5682
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6694
BstBI TT'CG_AA
Cuts at: 3764 5498 3764
Size: 1734 4960
BstEII G'GTnAC_C
Cuts at: 1184 2834 1184
Size: 1650 5044
BstXI CCAn_nnnn'nTGG
Cuts at: 3842 3842
Size: 6694
BstYI r'GATC_y
Cuts at: 1972 2389 3099 4024 4391 4776 4793 6245 6331
Size: 417 710 925 367 385 17 1452 86
Cuts at: 6331 6343 6689 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 925 710 417 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2664 3729 2664
Size: 1065 5629
BtgI C'CryG_G
Cuts at: 513 1065 3774 513
Size: 552 2709 3433
Fragments arranged by size:
3433 2709 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2787 487
Size: 683 56 1561 4394
Fragments arranged by size:
4394 1561 683 56
BtsI GCAGTG_nn'
Cuts at: 3242 4210 5004 5024 3242
Size: 968 794 20 4912
Fragments arranged by size:
4912 968 794 20
ClaI AT'CG_AT
Cuts at: 1554 2191 1554
Size: 637 6057
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2812 2847 529
Size: 35 2248 35 4376
Fragments arranged by size:
4376 2248 35 35
DraI TTT'AAA
Cuts at: 3998 3998
Size: 6694
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2072 5701 809
Size: 507 756 3629 1802
Fragments arranged by size:
3629 1802 756 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2128 4141 5030
Size: 54 1425 50 275 18 298 2013 889
Cuts at: 5030 6659 8
Size: 1629 43
Fragments arranged by size:
2013 1629 1425 889 298 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4141 1537
Size: 275 2329 4090
Fragments arranged by size:
4090 2329 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2331 2375 2608 2640 2756 3060 3224 4624
Size: 978 44 233 32 116 304 164 1400
Cuts at: 4624 1353
Size: 3423
Fragments arranged by size:
3423 1400 978 304 233 164 116 44
32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5281 5655 5801 387
Size: 1064 138 3692 374 146 1280
Fragments arranged by size:
3692 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2742 2742
Size: 6694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2406 2544 2720 3126 3233 3386
Size: 393 370 404 138 176 406 107 153
Cuts at: 3386 3680 3813 4757 5354 6141 6409 1239
Size: 294 133 944 597 787 268 1524
Fragments arranged by size:
1524 944 787 597 406 404 393 370
294 268 176 153 138 133 107
EcoO109I rG'GnC_Cy
Cuts at: 3370 3370
Size: 6694
EcoRI G'AATT_C
Cuts at: 4003 4381 4003
Size: 378 6316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2360 2360
Size: 6694
FspI TGC'GCA
Cuts at: 5200 5200
Size: 6694
HaeII r_GCGC'y
Cuts at: 1678 2990 5841 1678
Size: 1312 2851 2531
Fragments arranged by size:
2851 2531 1312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 2612 2644 5335
Size: 32 135 32 635 32 373 32 2691
Cuts at: 5335 5367 5409 5441 1373
Size: 32 42 32 2626
Fragments arranged by size:
2691 2626 635 373 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4038 4284 669
Size: 988 281 2100 246 3079
Fragments arranged by size:
3079 2100 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6694
HpaI GTT'AAC
Cuts at: 4284 4284
Size: 6694
KpnI G_GTAC'C
Cuts at: 2823 4019 2823
Size: 1196 5498
MfeI C'AATT_G
Cuts at: 4293 4293
Size: 6694
MluI A'CGCG_T
Cuts at: 6570 6570
Size: 6694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3317 5808 5992 1305
Size: 2012 2491 184 2007
Fragments arranged by size:
2491 2012 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2130 10
Size: 54 1425 343 298 4574
Fragments arranged by size:
4574 1425 343 298 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3932 4652 5011 5170 518
Size: 648 2766 720 359 159 2042
Fragments arranged by size:
2766 2042 720 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 3521 4110 4787 5935 6180 1606
Size: 452 1463 589 677 1148 245 2120
Fragments arranged by size:
2120 1463 1148 677 589 452 245
NcoI C'CATG_G
Cuts at: 513 1065 3774 513
Size: 552 2709 3433
Fragments arranged by size:
3433 2709 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6694
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6694
NotI GC'GGCC_GC
Cuts at: 4141 4141
Size: 6694
NsiI A_TGCA'T
Cuts at: 3478 3478
Size: 6694
NspI r_CATG'y
Cuts at: 1132 1229 4052 5597 1132
Size: 97 2823 1545 2229
Fragments arranged by size:
2823 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2026 1348
Size: 678 6016
PciI A'CATG_T
Cuts at: 5593 5593
Size: 6694
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2032 1108
Size: 902 22 5770
Fragments arranged by size:
5770 902 22
PmeI GTTT'AAAC
Cuts at: 3998 3998
Size: 6694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4763 4795 6276 6308 4763
Size: 32 1481 32 5149
Fragments arranged by size:
5149 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6694
PsiI TTA'TAA
Cuts at: 3977 4264 3977
Size: 287 6407
PspXI vC'TCGA_Gb
Cuts at: 2113 2113
Size: 6694
PstI C_TGCA'G
Cuts at: 830 4046 830
Size: 3216 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6694
PvuII CAG'CTG
Cuts at: 3521 3521
Size: 6694
SacI G_AGCT'C
Cuts at: 2744 2744
Size: 6694
SalI G'TCGA_C
Cuts at: 1936 4036 1936
Size: 2100 4594
SapI GCTCTTCn'nnn_
Cuts at: 2756 3224 2756
Size: 468 6226
SbfI CC_TGCA'GG
Cuts at: 4046 4046
Size: 6694
ScaI AGT'ACT
Cuts at: 4942 6654 4942
Size: 1712 4982
SfcI C'TryA_G
Cuts at: 826 1045 3311 4042 5177 5858 6049 826
Size: 219 2266 731 1135 681 191 1471
Fragments arranged by size:
2266 1471 1135 731 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6694
SmaI CCC'GGG
Cuts at: 1872 4021 1872
Size: 2149 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2113 2479 3860 4798 5699 5961
Size: 197 926 170 366 1381 938 901 262
Cuts at: 5961 820
Size: 1553
Fragments arranged by size:
1553 1381 938 926 901 366 262 197
170
SnaBI TAC'GTA
Cuts at: 493 4485 493
Size: 3992 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6694
SphI G_CATG'C
Cuts at: 1132 4052 1132
Size: 2920 3774
SspI AAT'ATT
Cuts at: 5 52 3901 4618 5
Size: 47 3849 717 2081
Fragments arranged by size:
3849 2081 717 47
StuI AGG'CCT
Cuts at: 1517 3090 1517
Size: 1573 5121
StyI C'CwwG_G
Cuts at: 513 1065 2608 2745 3774 4133 513
Size: 552 1543 137 1029 359 3074
Fragments arranged by size:
3074 1543 1029 552 359 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3693 4721 4738 4891 5076 5521 325
Size: 1255 2113 1028 17 153 185 445 1498
Fragments arranged by size:
2113 1498 1255 1028 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2133 3707 3814
Size: 274 80 33 51 1443 156 1574 107
Cuts at: 3814 4940 6652 96
Size: 1126 1712 138
Fragments arranged by size:
1712 1574 1443 1126 274 156 138 107
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2324 2356 6654 6686 2324
Size: 32 4298 32 2332
Fragments arranged by size:
4298 2332 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6694
XbaI T'CTAG_A
Cuts at: 4030 4030
Size: 6694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 3966 2167
Size: 1799 4895
XhoI C'TCGA_G
Cuts at: 1943 2113 1943
Size: 170 6524
XmaI C'CCGG_G
Cuts at: 1870 4019 1870
Size: 2149 4545
XmnI GAAnn'nnTTC
Cuts at: 3218 4823 3218
Size: 1605 5089
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2515 4502 276
Size: 53 83 186 936 981 1987 2468
Fragments arranged by size:
2468 1987 981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI