Restriction map for
pFN21AE1018
(Circular) (Six-base) MAPSORT of: pFN21AE1018.seq Check: 3388 from: 1 to: 6622
pFN21AE1018 6622 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4432 278
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2671 3965 1937
Size: 734 1294 4594
Fragments arranged by size:
4594 1294 734
Acc65I G'GTAC_C
Cuts at: 3943 3943
Size: 6622
AclI AA'CG_TT
Cuts at: 1563 4749 5122 1563
Size: 3186 373 3063
Fragments arranged by size:
3186 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2716 2884 3556 4685 6069 6337 1632
Size: 1084 168 672 1129 1384 268 1917
Fragments arranged by size:
1917 1384 1129 1084 672 268 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6425
AflIII A'CryG_T
Cuts at: 1203 3919 5521 6498 1203
Size: 2716 1602 977 1327
Fragments arranged by size:
2716 1602 1327 977
AgeI A'CCGG_T
Cuts at: 5453 5453
Size: 6622
AhdI GACnn_n'nnGTC
Cuts at: 5351 5351
Size: 6622
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6622
AlwNI CAG_nnn'CTG
Cuts at: 2018 2134 4365 5937 2018
Size: 116 2231 1572 2703
Fragments arranged by size:
2703 2231 1572 116
ApaLI G'TGCA_C
Cuts at: 4679 5835 4679
Size: 1156 5466
ApoI r'AATT_y
Cuts at: 1493 3606 3931 4157 4309 6418 1493
Size: 2113 325 226 152 2109 1697
Fragments arranged by size:
2113 2109 1697 325 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5176 160
Size: 872 4144 1606
Fragments arranged by size:
4144 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
AvaI C'yCGr_G
Cuts at: 1870 1943 3947 1870
Size: 73 2004 4545
Fragments arranged by size:
4545 2004 73
BamHI G'GATC_C
Cuts at: 3952 4319 3952
Size: 367 6255
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3943 5398 618
Size: 325 217 627 2156 1455 1842
Fragments arranged by size:
2156 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3941 1391
Size: 2550 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5651
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4813 4847 1903
Size: 34 2876 34 3678
Fragments arranged by size:
3678 2876 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4514 5724 4514
Size: 1210 5412
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6622
BfrBI ATG'CAT
Cuts at: 3716 3716
Size: 6622
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5233 136
Size: 107 122 71 631 605 3561 1525
Fragments arranged by size:
3561 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6617 6617
Size: 6622
BlpI GC'TnA_GC
Cuts at: 4039 4039
Size: 6622
Bme1580I G_kGCm'C
Cuts at: 1790 4683 5839 1790
Size: 2893 1156 2573
Fragments arranged by size:
2893 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2167 1318
Size: 849 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4353 5311 442
Size: 416 53 83 399 24 2936 958 1753
Fragments arranged by size:
2936 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 3048 3804 5282 1239
Size: 956 853 756 1478 2579
Fragments arranged by size:
2579 1478 956 853 756
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2219 4747 5612 5910 2219
Size: 2528 865 298 2931
Fragments arranged by size:
2931 2528 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2171 2860 5285 882
Size: 617 672 689 2425 2219
Fragments arranged by size:
2425 2219 689 672 617
BsaAI yAC'GTr
Cuts at: 493 1204 4413 493
Size: 711 3209 2702
Fragments arranged by size:
3209 2702 711
BsaBI GATnn'nnATC
Cuts at: 6616 6616
Size: 6622
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4429 4811 5446 275
Size: 53 83 186 936 2896 382 635 1451
Fragments arranged by size:
2896 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5055 5453 5727 5874 6285 5055
Size: 398 274 147 411 5392
Fragments arranged by size:
5392 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 1178
Size: 30 165 30 711 30 5656
Fragments arranged by size:
5656 711 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 1186
Size: 949 5673
BseYI C'CCAG_C
Cuts at: 5825 6321 5825
Size: 496 6126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6049
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4072 4833 5861 1540
Size: 275 186 2071 761 1028 2301
Fragments arranged by size:
2301 2071 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2407 4683 4768 5839 2407
Size: 2276 85 1071 3190
Fragments arranged by size:
3190 2276 1071 85
BsmI GAATG_Cn'
Cuts at: 2258 3050 4133 4226 2258
Size: 792 1083 93 4654
Fragments arranged by size:
4654 1083 792 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2157 2157
Size: 6622
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2407 3941 4683 4768 5839 1391
Size: 399 617 1534 742 85 1071 2174
Fragments arranged by size:
2174 1534 1071 742 617 399 85
BspEI T'CCGG_A
Cuts at: 6285 6285
Size: 6622
BspHI T'CATG_A
Cuts at: 2416 4511 6241 2416
Size: 2095 1730 2797
Fragments arranged by size:
2797 2095 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2200 3977 844
Size: 1356 1777 3489
Fragments arranged by size:
3489 1777 1356
BsrBI CCG'CTC
Cuts at: 1394 1823 4509 1394
Size: 429 2686 3507
Fragments arranged by size:
3507 2686 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3475 5117 5291 65
Size: 1183 2227 1642 174 1396
Fragments arranged by size:
2227 1642 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5266 5453 1700
Size: 110 3456 187 2869
Fragments arranged by size:
3456 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2157 2755 3343 3763 3803 3847 96
Size: 2061 598 588 420 40 44 2871
Fragments arranged by size:
2871 2061 598 588 420 44 40
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6622
BssSI C'ACGA_G
Cuts at: 2406 4682 5694 2406
Size: 2276 1012 3334
Fragments arranged by size:
3334 2276 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6622
BstBI TT'CG_AA
Cuts at: 5426 5426
Size: 6622
BstEII G'GTnAC_C
Cuts at: 1184 2212 1184
Size: 1028 5594
BstYI r'GATC_y
Cuts at: 1972 3952 4319 4704 4721 6173 6259 6271 6617
Size: 1980 367 385 17 1452 86 12 346
Cuts at: 6617 1972
Size: 1977
Fragments arranged by size:
1980 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3880 513
Size: 552 2815 3255
Fragments arranged by size:
3255 2815 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5883
Fragments arranged by size:
5883 683 56
BtsI GCAGTG_nn'
Cuts at: 4138 4932 4952 4138
Size: 794 20 5808
Fragments arranged by size:
5808 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6587
DraI TTT'AAA
Cuts at: 3926 3926
Size: 6622
DraIII CAC_nnn'GTG
Cuts at: 3726 3726
Size: 6622
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5629 809
Size: 507 4313 1802
Fragments arranged by size:
4313 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4069 4958 6587
Size: 54 1425 50 275 18 2239 889 1629
Cuts at: 6587 8
Size: 43
Fragments arranged by size:
2239 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4069 1537
Size: 275 2257 4090
Fragments arranged by size:
4090 2257 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3880 4552 1353
Size: 2527 672 3423
Fragments arranged by size:
3423 2527 672
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5209 5583 5729 387
Size: 1064 138 3620 374 146 1280
Fragments arranged by size:
3620 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2716 2884 3048 3556 3804 4685
Size: 393 563 521 168 164 508 248 881
Cuts at: 4685 5282 6069 6337 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 881 787 597 563 521 508 393
268 248 168 164
EcoO109I rG'GnC_Cy
Cuts at: 2151 2151
Size: 6622
EcoRI G'AATT_C
Cuts at: 3606 3931 4309 3606
Size: 325 378 5919
Fragments arranged by size:
5919 378 325
FspI TGC'GCA
Cuts at: 5128 5128
Size: 6622
HaeII r_GCGC'y
Cuts at: 1678 5769 1678
Size: 4091 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2314 2346 5263 5295 5337
Size: 32 135 32 742 32 2917 32 42
Cuts at: 5337 5369 1373
Size: 32 2626
Fragments arranged by size:
2917 2626 742 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2913 3165 3249 3753 3966 4212
Size: 988 281 975 252 84 504 213 246
Cuts at: 4212 669
Size: 3079
Fragments arranged by size:
3079 988 975 504 281 252 246 213
84
HindIII A'AGCT_T
Cuts at: 748 2041 2811 3077 3651 748
Size: 1293 770 266 574 3719
Fragments arranged by size:
3719 1293 770 574 266
HpaI GTT'AAC
Cuts at: 4212 4212
Size: 6622
KpnI G_GTAC'C
Cuts at: 3947 3947
Size: 6622
MfeI C'AATT_G
Cuts at: 4221 4221
Size: 6622
MluI A'CGCG_T
Cuts at: 6498 6498
Size: 6622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 2347 3021 3626 5736 5920 1305
Size: 915 127 674 605 2110 184 2007
Fragments arranged by size:
2110 2007 915 674 605 184 127
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4800
Fragments arranged by size:
4800 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2334 4580 4939 5098 518
Size: 648 1168 2246 359 159 2042
Fragments arranged by size:
2246 2042 1168 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4038 4715 5863 6108 1606
Size: 2432 677 1148 245 2120
Fragments arranged by size:
2432 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3880 513
Size: 552 2815 3255
Fragments arranged by size:
3255 2815 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6622
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6622
NotI GC'GGCC_GC
Cuts at: 4069 4069
Size: 6622
NsiI A_TGCA'T
Cuts at: 3718 3718
Size: 6622
NspI r_CATG'y
Cuts at: 1132 1229 2721 3720 3923 3980 5525 1132
Size: 97 1492 999 203 57 1545 2229
Fragments arranged by size:
2229 1545 1492 999 203 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6622
PciI A'CATG_T
Cuts at: 3919 5521 3919
Size: 1602 5020
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6622
PfoI T'CCnGG_A
Cuts at: 2605 2605
Size: 6622
PmeI GTTT'AAAC
Cuts at: 3926 3926
Size: 6622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 6204 6236 4691
Size: 32 1481 32 5077
Fragments arranged by size:
5077 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2151 2151
Size: 6622
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6622
PsiI TTA'TAA
Cuts at: 4192 4192
Size: 6622
PstI C_TGCA'G
Cuts at: 830 2542 3974 830
Size: 1712 1432 3478
Fragments arranged by size:
3478 1712 1432
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6622
SalI G'TCGA_C
Cuts at: 1936 3964 1936
Size: 2028 4594
SbfI CC_TGCA'GG
Cuts at: 3974 3974
Size: 6622
ScaI AGT'ACT
Cuts at: 4870 6582 4870
Size: 1712 4910
SfcI C'TryA_G
Cuts at: 826 1045 2182 2538 3970 5105 5786 5977 826
Size: 219 1137 356 1432 1135 681 191 1471
Fragments arranged by size:
1471 1432 1137 1135 681 356 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6622
SmaI CCC'GGG
Cuts at: 1872 3949 1872
Size: 2077 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 4726 5627 5889 820
Size: 197 926 291 2492 901 262 1553
Fragments arranged by size:
2492 1553 926 901 291 262 197
SnaBI TAC'GTA
Cuts at: 493 4413 493
Size: 3920 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6622
SphI G_CATG'C
Cuts at: 1132 3720 3980 1132
Size: 2588 260 3774
Fragments arranged by size:
3774 2588 260
SspI AAT'ATT
Cuts at: 5 52 4546 5
Size: 47 4494 2081
Fragments arranged by size:
4494 2081 47
StuI AGG'CCT
Cuts at: 1517 2664 1517
Size: 1147 5475
StyI C'CwwG_G
Cuts at: 513 1065 3337 3880 4061 513
Size: 552 2272 543 181 3074
Fragments arranged by size:
3074 2272 552 543 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2129 4649 4666 4819 5004 5449 325
Size: 1255 549 2520 17 153 185 445 1498
Fragments arranged by size:
2520 1498 1255 549 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 2444 2711
Size: 274 80 33 51 1443 180 287 267
Cuts at: 2711 2755 3215 3343 3763 3803 3847 4868 6580
Size: 44 460 128 420 40 44 1021 1712
Cuts at: 6580 96
Size: 138
Fragments arranged by size:
1712 1443 1021 460 420 287 274 267
180 138 128 80 51 44 44 40
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6582 6614 6582
Size: 32 6590
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6622
XbaI T'CTAG_A
Cuts at: 3958 3958
Size: 6622
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6622
XmaI C'CCGG_G
Cuts at: 1870 3947 1870
Size: 2077 4545
XmnI GAAnn'nnTTC
Cuts at: 2729 3149 3233 3358 3401 4751 2729
Size: 420 84 125 43 1350 4600
Fragments arranged by size:
4600 1350 420 125 84 43
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4430 276
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI