Restriction map for
pFN21AE1015
(Circular) (Six-base) MAPSORT of: pFN21AE1015.seq Check: 9508 from: 1 to: 5695
pFN21AE1015 5695 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2489 3758 5142 5410 1632
Size: 857 1269 1384 268 1917
Fragments arranged by size:
1917 1384 1269 857 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 2050 4594 5571 1203
Size: 847 2544 977 1327
Fragments arranged by size:
2544 1327 977 847
AgeI A'CCGG_T
Cuts at: 2565 4526 2565
Size: 1961 3734
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AlwNI CAG_nnn'CTG
Cuts at: 3438 5010 3438
Size: 1572 4123
ApaI G_GGCC'C
Cuts at: 2929 2929
Size: 5695
ApaLI G'TGCA_C
Cuts at: 2236 3752 4908 2236
Size: 1516 1156 3023
Fragments arranged by size:
3023 1516 1156
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 3020 1870
Size: 73 1077 4545
Fragments arranged by size:
4545 1077 73
BamHI G'GATC_C
Cuts at: 2040 2673 2940 3025 3392 2040
Size: 633 267 85 367 4343
Fragments arranged by size:
4343 633 367 267 85
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2899 3016 4471 618
Size: 325 217 627 357 755 117 1455 1842
Fragments arranged by size:
1842 1455 755 627 357 325 217 117
BanII G_rGCy'C
Cuts at: 1391 2079 2170 2190 2483 2929 3014 1391
Size: 688 91 20 293 446 85 4072
Fragments arranged by size:
4072 688 446 293 91 85 20
BbeI G_GCGC'C
Cuts at: 2903 2903
Size: 5695
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2603 2637 3886 3920 1903
Size: 34 666 34 1249 34 3678
Fragments arranged by size:
3678 1249 666 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2831 2852 2933
Size: 107 122 71 631 605 1159 21 81
Cuts at: 2933 4306 136
Size: 1373 1525
Fragments arranged by size:
1525 1373 1159 631 605 122 107 81
71 21
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 3112 3112
Size: 5695
Bme1580I G_kGCm'C
Cuts at: 1790 2147 2240 2929 3756 4912 1790
Size: 357 93 689 827 1156 2573
Fragments arranged by size:
2573 1156 827 689 357 93
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5695
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2257 3426 4384
Size: 416 53 83 399 24 840 1169 958
Cuts at: 4384 442
Size: 1753
Fragments arranged by size:
1753 1169 958 840 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2093 2093
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 2900 2951 4355 1239
Size: 1130 531 51 1404 2579
Fragments arranged by size:
2579 1404 1130 531 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2615 3820 4685 4983 2615
Size: 1205 865 298 3327
Fragments arranged by size:
3327 1205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2219 4358 882
Size: 617 720 2139 2219
Fragments arranged by size:
2219 2139 720 617
BsaAI yAC'GTr
Cuts at: 493 1204 2490 3486 493
Size: 711 1286 996 2702
Fragments arranged by size:
2702 1286 996 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2421 2900 3502 3884
Size: 53 83 186 936 888 479 602 382
Cuts at: 3884 4519 275
Size: 635 1451
Fragments arranged by size:
1451 936 888 635 602 479 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2118 2565 4128 4526 4800 4947 5358 2118
Size: 447 1563 398 274 147 411 2455
Fragments arranged by size:
2455 1563 447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2085 2753 1186
Size: 899 668 4128
Fragments arranged by size:
4128 899 668
BseYI C'CCAG_C
Cuts at: 2439 2890 4898 5394 2439
Size: 451 2008 496 2740
Fragments arranged by size:
2740 2008 496 451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2111 1163
Size: 573 375 4747
Fragments arranged by size:
4747 573 375
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2835 3145 3906 4934 1540
Size: 275 186 834 310 761 1028 2301
Fragments arranged by size:
2301 1028 834 761 310 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2190 2240 3756 3841 4912 2079
Size: 111 50 1516 85 1071 2862
Fragments arranged by size:
2862 1516 1071 111 85 50
BsmI GAATG_Cn'
Cuts at: 2848 3206 3299 2848
Size: 358 93 5244
Fragments arranged by size:
5244 358 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2147 2170 2190 2240 2483 2929
Size: 399 289 68 23 20 50 243 446
Cuts at: 2929 3014 3756 3841 4912 1391
Size: 85 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 446 399 289 243 85
85 68 50 23 20
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2646 3050 844
Size: 1802 404 3489
Fragments arranged by size:
3489 1802 404
BsrBI CCG'CTC
Cuts at: 1394 1823 3582 1394
Size: 429 1759 3507
Fragments arranged by size:
3507 1759 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2619 4190 4364 65
Size: 1183 1371 1571 174 1396
Fragments arranged by size:
1571 1396 1371 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2565 4339 4526 1700
Size: 110 755 1774 187 2869
Fragments arranged by size:
2869 1774 755 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 2169 3755 4767 2169
Size: 1586 1012 3097
Fragments arranged by size:
3097 1586 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5695
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5695
BstXI CCAn_nnnn'nTGG
Cuts at: 2878 2878
Size: 5695
BstYI r'GATC_y
Cuts at: 1972 2040 2673 2940 2982 3025 3392 3777 3794
Size: 68 633 267 42 43 367 385 17
Cuts at: 3794 5246 5332 5344 5690 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 633 385 367 346 267 86
68 43 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2181 2504 513
Size: 552 939 177 323 3704
Fragments arranged by size:
3704 939 552 323 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2002 2311 487
Size: 683 56 776 309 3871
Fragments arranged by size:
3871 776 683 309 56
BtsI GCAGTG_nn'
Cuts at: 2362 2544 2761 3211 4005 4025 2362
Size: 182 217 450 794 20 4032
Fragments arranged by size:
4032 794 450 217 182 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2795 4702 809
Size: 507 1479 1907 1802
Fragments arranged by size:
1907 1802 1479 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2307 2832 3142
Size: 54 1425 50 275 18 477 525 310
Cuts at: 3142 4031 5660 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 525 477 310 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2832 3142 1537
Size: 275 1020 310 4090
Fragments arranged by size:
4090 1020 310 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2517 2773 2952 3625 1353
Size: 1164 256 179 673 3423
Fragments arranged by size:
3423 1164 673 256 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4282 4656 4802 387
Size: 1064 138 435 2258 374 146 1280
Fragments arranged by size:
2258 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2077 2188 2077
Size: 111 5584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2489 2900 2951 3758 4355 5142
Size: 393 737 120 411 51 807 597 787
Cuts at: 5142 5410 1239
Size: 268 1524
Fragments arranged by size:
1524 807 787 737 597 411 393 268
120 51
EcoO109I rG'GnC_Cy
Cuts at: 2341 2500 2661 2926 2341
Size: 159 161 265 5110
Fragments arranged by size:
5110 265 161 159
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
EcoRV GAT'ATC
Cuts at: 2014 2014
Size: 5695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 5695
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 2903 4842 1678
Size: 1225 1939 2531
Fragments arranged by size:
2531 1939 1225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2750 2782 4336 4368 4410
Size: 32 135 32 1178 32 1554 32 42
Cuts at: 4410 4442 1373
Size: 32 2626
Fragments arranged by size:
2626 1554 1178 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KasI G'GCGC_C
Cuts at: 2899 2899
Size: 5695
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4809 4993 1305
Size: 3504 184 2007
Fragments arranged by size:
3504 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2309 10
Size: 54 1425 343 477 3396
Fragments arranged by size:
3396 1425 477 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2330 2462 3653 4012 4171 518
Size: 648 1164 132 1191 359 159 2042
Fragments arranged by size:
2042 1191 1164 648 359 159 132
MspA1I CmG'CkG
Cuts at: 1606 2302 2359 2680 2701 3111 3788 4936 5181
Size: 696 57 321 21 410 677 1148 245
Cuts at: 5181 1606
Size: 2120
Fragments arranged by size:
2120 1148 696 677 410 321 245 57
21
NarI GG'CG_CC
Cuts at: 2900 2900
Size: 5695
NcoI C'CATG_G
Cuts at: 513 1065 2004 2181 2504 513
Size: 552 939 177 323 3704
Fragments arranged by size:
3704 939 552 323 177
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 2288 2588 3053 4598 1132
Size: 97 1059 300 465 1545 2229
Fragments arranged by size:
2229 1545 1059 465 300 97
PasI CC'CwG_GG
Cuts at: 1348 2401 1348
Size: 1053 4642
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5695
PfoI T'CCnGG_A
Cuts at: 2028 2079 2028
Size: 51 5644
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2341 2500 2661 2341
Size: 159 161 5375
Fragments arranged by size:
5375 161 159
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5695
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PspOMI G'GGCC_C
Cuts at: 2925 2925
Size: 5695
PstI C_TGCA'G
Cuts at: 830 2430 2643 3047 830
Size: 1600 213 404 3478
Fragments arranged by size:
3478 1600 404 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
PvuII CAG'CTG
Cuts at: 2680 2680
Size: 5695
SacI G_AGCT'C
Cuts at: 2079 2190 2079
Size: 111 5584
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SanDI GG'GwC_CC
Cuts at: 2661 2661
Size: 5695
SapI GCTCTTCn'nnn_
Cuts at: 2773 2773
Size: 5695
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 2609 2744 3943 5655 2609
Size: 135 1199 1712 2649
Fragments arranged by size:
2649 1712 1199 135
SfcI C'TryA_G
Cuts at: 826 1045 2426 2639 3043 4178 4859 5050 826
Size: 219 1381 213 404 1135 681 191 1471
Fragments arranged by size:
1471 1381 1135 681 404 219 213 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2831 2831
Size: 5695
SfoI GGC'GCC
Cuts at: 2901 2901
Size: 5695
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 3022 1872
Size: 1150 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2630 3799 4700 4962 820
Size: 197 926 687 1169 901 262 1553
Fragments arranged by size:
1553 1169 926 901 687 262 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 2804 1517
Size: 1287 4408
StyI C'CwwG_G
Cuts at: 513 1065 2004 2100 2181 2504 3134 513
Size: 552 939 96 81 323 630 3074
Fragments arranged by size:
3074 939 630 552 323 96 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3722 3739 3892 4077 4522 325
Size: 1255 2142 17 153 185 445 1498
Fragments arranged by size:
2142 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2607 2742 3941
Size: 274 80 33 51 1443 630 135 1199
Cuts at: 3941 5653 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1199 630 274 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2753 2785 5655 5687 2753
Size: 32 2870 32 2761
Fragments arranged by size:
2870 2761 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2795 1316
Size: 1479 4216
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 3020 1870
Size: 1150 4545
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SexAI SrfI SwaI XcmI