Restriction map for
pFN21AE1013
(Circular) (Six-base) MAPSORT of: pFN21AE1013.seq Check: 1064 from: 1 to: 5560
pFN21AE1013 5560 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3370 278
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2289 2903 1937
Size: 352 614 4594
Fragments arranged by size:
4594 614 352
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3623 5007 5275 1632
Size: 1991 1384 268 1917
Fragments arranged by size:
1991 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5363
AflIII A'CryG_T
Cuts at: 1203 2367 4459 5436 1203
Size: 1164 2092 977 1327
Fragments arranged by size:
2092 1327 1164 977
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 2426 4289 2426
Size: 1863 3697
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5560
AlwNI CAG_nnn'CTG
Cuts at: 3303 4875 3303
Size: 1572 3988
ApaLI G'TGCA_C
Cuts at: 2354 3617 4773 2354
Size: 1263 1156 3141
Fragments arranged by size:
3141 1263 1156
ApoI r'AATT_y
Cuts at: 1493 2869 3095 3247 5356 1493
Size: 1376 226 152 2109 1697
Fragments arranged by size:
2109 1697 1376 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2192 2885 1870
Size: 73 249 693 4545
Fragments arranged by size:
4545 693 249 73
BamHI G'GATC_C
Cuts at: 2890 3257 2890
Size: 367 5193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2881 4336 618
Size: 325 217 627 1094 1455 1842
Fragments arranged by size:
1842 1455 1094 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2655 2879 1391
Size: 1264 224 4072
Fragments arranged by size:
4072 1264 224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4589
BbvCI CC'TCA_GC
Cuts at: 2618 2618
Size: 5560
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 1903
Size: 34 1814 34 3678
Fragments arranged by size:
3678 1814 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2533 3452 4662 2533
Size: 919 1210 3431
Fragments arranged by size:
3431 1210 919
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4171 136
Size: 107 122 71 631 605 2499 1525
Fragments arranged by size:
2499 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5555 5555
Size: 5560
BlpI GC'TnA_GC
Cuts at: 2977 2977
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 1790 2056 2358 2502 2637 3621 4777 1790
Size: 266 302 144 135 984 1156 2573
Fragments arranged by size:
2573 1156 984 302 266 144 135
BmgBI CAC'GTC
Cuts at: 1318 2683 1318
Size: 1365 4195
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4249 442
Size: 416 53 83 399 24 1874 958 1753
Fragments arranged by size:
1874 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2263 2771 2843 4220 1239
Size: 1024 508 72 1377 2579
Fragments arranged by size:
2579 1377 1024 508 72
Bpu10I CC'TnA_GC
Cuts at: 2618 2618
Size: 5560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4550 4848 3685
Size: 865 298 4397
Fragments arranged by size:
4397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4223 882
Size: 617 2724 2219
Fragments arranged by size:
2724 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3351 493
Size: 711 2147 2702
Fragments arranged by size:
2702 2147 711
BsaBI GATnn'nnATC
Cuts at: 5554 5554
Size: 5560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2595 2727 3367 3749
Size: 53 83 186 936 1062 132 640 382
Cuts at: 3749 4384 275
Size: 635 1451
Fragments arranged by size:
1451 1062 936 640 635 382 186 132
83 53
BsaWI w'CCGG_w
Cuts at: 3993 4391 4665 4812 5223 3993
Size: 398 274 147 411 4330
Fragments arranged by size:
4330 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5335
Fragments arranged by size:
5335 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2031 2034 2054 2057 2225 1186
Size: 845 3 20 3 168 4521
Fragments arranged by size:
4521 845 168 20 3 3
BseYI C'CCAG_C
Cuts at: 4763 5259 4763
Size: 496 5064
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2029 2813 1163
Size: 573 293 784 3910
Fragments arranged by size:
3910 784 573 293
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3010 3771 4799 1540
Size: 275 186 1009 761 1028 2301
Fragments arranged by size:
2301 1028 1009 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 2358 2610 2655 3621 3706 4777 2193
Size: 165 252 45 966 85 1071 2976
Fragments arranged by size:
2976 1071 966 252 165 85 45
BsmI GAATG_Cn'
Cuts at: 2316 3071 3164 2316
Size: 755 93 4712
Fragments arranged by size:
4712 755 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2747 2747
Size: 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2193 2358 2502 2610 2637 2655
Size: 399 266 137 165 144 108 27 18
Cuts at: 2655 2879 3621 3706 4777 1391
Size: 224 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 266 224 165 144
137 108 85 27 18
BspEI T'CCGG_A
Cuts at: 5223 5223
Size: 5560
BspHI T'CATG_A
Cuts at: 2490 3449 5179 2490
Size: 959 1730 2871
Fragments arranged by size:
2871 1730 959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2915 844
Size: 2071 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2788 3447 1394
Size: 429 965 659 3507
Fragments arranged by size:
3507 965 659 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4055 4229 65
Size: 1183 2807 174 1396
Fragments arranged by size:
2807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2557 4204 4391 1700
Size: 110 747 1647 187 2869
Fragments arranged by size:
2869 1647 747 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5560
BssSI C'ACGA_G
Cuts at: 3620 4632 3620
Size: 1012 4548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5560
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2890 3257 3642 3659 5111 5197 5209 5555
Size: 918 367 385 17 1452 86 12 346
Cuts at: 5555 1972
Size: 1977
Fragments arranged by size:
1977 1452 918 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5008
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 2222 3076 3870 3890 2222
Size: 854 794 20 3892
Fragments arranged by size:
3892 854 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5525
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4567 809
Size: 507 3251 1802
Fragments arranged by size:
3251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2587 2718 3007
Size: 54 1425 50 275 18 757 131 289
Cuts at: 3007 3896 5525 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 757 289 275 131 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3007 1537
Size: 275 1195 4090
Fragments arranged by size:
4090 1195 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2522 2760 3490 1353
Size: 1169 238 730 3423
Fragments arranged by size:
3423 1169 730 238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4147 4521 4667 387
Size: 1064 138 2558 374 146 1280
Fragments arranged by size:
2558 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2653 2653
Size: 5560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2263 2771 2843 3623 4220 5007 5275
Size: 393 631 508 72 780 597 787 268
Cuts at: 5275 1239
Size: 1524
Fragments arranged by size:
1524 787 780 631 597 508 393 268
72
EcoNI CCTnn'n_nnAGG
Cuts at: 2519 2519
Size: 5560
EcoO109I rG'GnC_Cy
Cuts at: 2065 2141 2251 2776 2065
Size: 76 110 525 4849
Fragments arranged by size:
4849 525 110 76
EcoRI G'AATT_C
Cuts at: 2869 3247 2869
Size: 378 5182
FspI TGC'GCA
Cuts at: 2032 4066 2032
Size: 2034 3526
HaeII r_GCGC'y
Cuts at: 1678 4707 1678
Size: 3029 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 2412 2444 4201
Size: 32 135 32 689 32 119 32 1757
Cuts at: 4201 4233 4275 4307 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1757 689 135 119 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2904 3150 669
Size: 988 281 966 246 3079
Fragments arranged by size:
3079 988 966 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4674 4858 1305
Size: 3369 184 2007
Fragments arranged by size:
3369 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3738
Fragments arranged by size:
3738 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 3518 3877 4036 518
Size: 648 1329 1023 359 159 2042
Fragments arranged by size:
2042 1329 1023 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2392 2976 3653 4801 5046 1606
Size: 786 584 677 1148 245 2120
Fragments arranged by size:
2120 1148 786 677 584 245
NaeI GCC'GGC
Cuts at: 2559 2559
Size: 5560
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5008
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5560
NgoMIV G'CCGG_C
Cuts at: 2557 2557
Size: 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2833 2918 4463 1132
Size: 97 1604 85 1545 2229
Fragments arranged by size:
2229 1604 1545 97 85
PasI CC'CwG_GG
Cuts at: 1348 2056 1348
Size: 708 4852
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5560
PfoI T'CCnGG_A
Cuts at: 2520 2676 2520
Size: 156 5404
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2592 2624 3629 3661 5142 5174 2592
Size: 32 1005 32 1481 32 2978
Fragments arranged by size:
2978 1481 1005 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2141 2251 2141
Size: 110 5450
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PstI C_TGCA'G
Cuts at: 830 2229 2912 830
Size: 1399 683 3478
Fragments arranged by size:
3478 1399 683
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
SacI G_AGCT'C
Cuts at: 2655 2655
Size: 5560
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SanDI GG'GwC_CC
Cuts at: 2141 2141
Size: 5560
SbfI CC_TGCA'GG
Cuts at: 2912 2912
Size: 5560
ScaI AGT'ACT
Cuts at: 3808 5520 3808
Size: 1712 3848
SfcI C'TryA_G
Cuts at: 826 1045 2225 2908 4043 4724 4915 826
Size: 219 1180 683 1135 681 191 1471
Fragments arranged by size:
1471 1180 1135 683 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2887 1872
Size: 1015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3664 4565 4827 820
Size: 197 926 1721 901 262 1553
Fragments arranged by size:
1721 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2918 1132
Size: 1786 3774
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5560
StyI C'CwwG_G
Cuts at: 513 1065 2999 513
Size: 552 1934 3074
Fragments arranged by size:
3074 1934 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3587 3604 3757 3942 4387 325
Size: 1255 2007 17 153 185 445 1498
Fragments arranged by size:
2007 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3806 5518 96
Size: 274 80 33 51 1443 1829 1712 138
Fragments arranged by size:
1829 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5520 5552 5520
Size: 32 5528
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5560
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2419 2419
Size: 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2885 1870
Size: 1015 4545
XmnI GAAnn'nnTTC
Cuts at: 2204 2369 3689 2204
Size: 165 1320 4075
Fragments arranged by size:
4075 1320 165
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3368 276
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI