Restriction map for
pFN21AE1012
(Circular) (Six-base) MAPSORT of: pFN21AE1012.seq Check: 4818 from: 1 to: 5611
pFN21AE1012 5611 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2650 2686 2650
Size: 36 5575
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3421 278
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2954 1937
Size: 1017 4594
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 3738 4111 1563
Size: 2175 373 3063
Fragments arranged by size:
3063 2175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3674 5058 5326 1632
Size: 2042 1384 268 1917
Fragments arranged by size:
2042 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5414
AflIII A'CryG_T
Cuts at: 1203 4510 5487 1203
Size: 3307 977 1327
Fragments arranged by size:
3307 1327 977
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 4340 4340
Size: 5611
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2724 2832 1184
Size: 1540 108 3963
Fragments arranged by size:
3963 1540 108
AlwNI CAG_nnn'CTG
Cuts at: 2395 2520 3354 4926 2395
Size: 125 834 1572 3080
Fragments arranged by size:
3080 1572 834 125
ApaI G_GGCC'C
Cuts at: 2062 2062
Size: 5611
ApaLI G'TGCA_C
Cuts at: 3668 4824 3668
Size: 1156 4455
ApoI r'AATT_y
Cuts at: 1493 2186 2920 3146 3298 5407 1493
Size: 693 734 226 152 2109 1697
Fragments arranged by size:
2109 1697 734 693 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2081 2305 2936 1870
Size: 73 125 13 224 631 4545
Fragments arranged by size:
4545 631 224 125 73 13
BamHI G'GATC_C
Cuts at: 2941 3308 2941
Size: 367 5244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2598 2932 4387 618
Size: 325 217 627 811 334 1455 1842
Fragments arranged by size:
1842 1455 811 627 334 325 217
BanII G_rGCy'C
Cuts at: 1391 2062 2357 2930 1391
Size: 671 295 573 4072
Fragments arranged by size:
4072 671 573 295
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2305 928
Size: 971 406 4234
Fragments arranged by size:
4234 971 406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3802 3836 1903
Size: 34 1865 34 3678
Fragments arranged by size:
3678 1865 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3503 4713 3503
Size: 1210 4401
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4222 136
Size: 107 122 71 631 605 2550 1525
Fragments arranged by size:
2550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5606 5606
Size: 5611
BlpI GC'TnA_GC
Cuts at: 3028 3028
Size: 5611
Bme1580I G_kGCm'C
Cuts at: 1790 2062 2601 2620 3672 4828 1790
Size: 272 539 19 1052 1156 2573
Fragments arranged by size:
2573 1156 1052 539 272 19
BmgBI CAC'GTC
Cuts at: 1318 2824 1318
Size: 1506 4105
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2195 2722 3342
Size: 416 53 83 399 24 778 527 620
Cuts at: 3342 4300 442
Size: 958 1753
Fragments arranged by size:
1753 958 778 620 527 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 2270 2279 2481 2603 4271 1239
Size: 1001 30 9 202 122 1668 2579
Fragments arranged by size:
2579 1668 1001 202 122 30 9
Bpu10I CC'TnA_GC
Cuts at: 2213 2213
Size: 5611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3736 4601 4899 3736
Size: 865 298 4448
Fragments arranged by size:
4448 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2437 2531 4274 882
Size: 617 938 94 1743 2219
Fragments arranged by size:
2219 1743 938 617 94
BsaAI yAC'GTr
Cuts at: 493 1204 3402 493
Size: 711 2198 2702
Fragments arranged by size:
2702 2198 711
BsaBI GATnn'nnATC
Cuts at: 5605 5605
Size: 5611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3418 3800 4435 275
Size: 53 83 186 936 1885 382 635 1451
Fragments arranged by size:
1885 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4044 4442 4716 4863 5274 4044
Size: 398 274 147 411 4381
Fragments arranged by size:
4381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5386
Fragments arranged by size:
5386 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2365 2705 1186
Size: 1179 340 4092
Fragments arranged by size:
4092 1179 340
BseYI C'CCAG_C
Cuts at: 2369 2541 2545 2626 4814 5310 2369
Size: 172 4 81 2188 496 2670
Fragments arranged by size:
2670 2188 496 172 81 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2720 2846 1163
Size: 573 984 126 3928
Fragments arranged by size:
3928 984 573 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2103 2322 3061 3822 4850 1540
Size: 275 186 102 219 739 761 1028 2301
Fragments arranged by size:
2301 1028 761 739 275 219 186 102
BsiHKAI G_wGCw'C
Cuts at: 2708 3672 3757 4828 2708
Size: 964 85 1071 3491
Fragments arranged by size:
3491 1071 964 85
BsmI GAATG_Cn'
Cuts at: 3122 3215 3122
Size: 93 5518
BsmBI CGTCTCn'nnnn_
Cuts at: 2106 2106
Size: 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2357 2601 2620 2708 2930 3672
Size: 399 272 295 244 19 88 222 742
Cuts at: 3672 3757 4828 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 295 272 244 222
88 85 19
BspEI T'CCGG_A
Cuts at: 5274 5274
Size: 5611
BspHI T'CATG_A
Cuts at: 3500 5230 3500
Size: 1730 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2650 2686 2966 844
Size: 1806 36 280 3489
Fragments arranged by size:
3489 1806 280 36
BsrBI CCG'CTC
Cuts at: 1394 1823 2095 3498 1394
Size: 429 272 1403 3507
Fragments arranged by size:
3507 1403 429 272
BsrDI GCAATG_nn'
Cuts at: 65 1248 4106 4280 65
Size: 1183 2858 174 1396
Fragments arranged by size:
2858 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2102 2321 2644 4255 4442 1700
Size: 110 292 219 323 1611 187 2869
Fragments arranged by size:
2869 1611 323 292 219 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5611
BssHII G'CGCG_C
Cuts at: 1925 2036 2118 1925
Size: 111 82 5418
Fragments arranged by size:
5418 111 82
BssSI C'ACGA_G
Cuts at: 3671 4683 3671
Size: 1012 4599
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5611
BstBI TT'CG_AA
Cuts at: 4415 4415
Size: 5611
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5611
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2369 2800 2011
Size: 358 431 4822
Fragments arranged by size:
4822 431 358
BstYI r'GATC_y
Cuts at: 1972 2941 3308 3693 3710 5162 5248 5260 5606
Size: 969 367 385 17 1452 86 12 346
Cuts at: 5606 1972
Size: 1977
Fragments arranged by size:
1977 1452 969 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2278 2787 513
Size: 552 939 274 509 3337
Fragments arranged by size:
3337 939 552 509 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2539 2829 487
Size: 683 56 1313 290 3269
Fragments arranged by size:
3269 1313 683 290 56
BtsI GCAGTG_nn'
Cuts at: 2706 3127 3921 3941 2706
Size: 421 794 20 4376
Fragments arranged by size:
4376 794 421 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5576
DraI TTT'AAA
Cuts at: 2915 2915
Size: 5611
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2681 4618 809
Size: 507 1365 1937 1802
Fragments arranged by size:
1937 1802 1365 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2319 2736
Size: 54 1425 50 275 18 270 219 417
Cuts at: 2736 2790 3058 3947 5576 8
Size: 54 268 889 1629 43
Fragments arranged by size:
1629 1425 889 417 275 270 268 219
54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2100 2319 3058 1537
Size: 275 288 219 739 4090
Fragments arranged by size:
4090 739 288 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2642 3541 1353
Size: 1289 899 3423
Fragments arranged by size:
3423 1289 899
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2101 2124 2425 2519 2624 2663
Size: 1064 138 512 23 301 94 105 39
Cuts at: 2663 4198 4572 4718 387
Size: 1535 374 146 1280
Fragments arranged by size:
1535 1280 1064 512 374 301 146 138
105 94 39 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2270 2279 2481 2603 3674 4271
Size: 393 608 30 9 202 122 1071 597
Cuts at: 4271 5058 5326 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1071 787 608 597 393 268 202
122 30 9
EcoO109I rG'GnC_Cy
Cuts at: 2058 2653 2058
Size: 595 5016
EcoRI G'AATT_C
Cuts at: 2920 3298 2920
Size: 378 5233
FseI GG_CCGG'CC
Cuts at: 2325 2325
Size: 5611
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 2012 2128 4758 1678
Size: 334 116 2630 2531
Fragments arranged by size:
2630 2531 334 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4252 4284 4326 4358 1373
Size: 32 135 32 2680 32 42 32 2626
Fragments arranged by size:
2680 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2955 3201 669
Size: 988 281 1017 246 3079
Fragments arranged by size:
3079 1017 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5611
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 3210 3210
Size: 5611
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2442 4725 4909 1305
Size: 1137 2283 184 2007
Fragments arranged by size:
2283 2007 1137 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2738 10
Size: 54 1425 343 906 2883
Fragments arranged by size:
2883 1425 906 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3569 3928 4087 518
Size: 648 2403 359 159 2042
Fragments arranged by size:
2403 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2140 2280 2459 2545 2626 2761 3027 3704
Size: 534 140 179 86 81 135 266 677
Cuts at: 3704 4852 5097 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 534 266 245 179 140
135 86 81
NaeI GCC'GGC
Cuts at: 2104 2323 2646 2104
Size: 219 323 5069
Fragments arranged by size:
5069 323 219
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4120
Fragments arranged by size:
4120 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NgoMIV G'CCGG_C
Cuts at: 2102 2321 2644 2102
Size: 219 323 5069
Fragments arranged by size:
5069 323 219
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NspI r_CATG'y
Cuts at: 1132 1229 2969 4514 1132
Size: 97 1740 1545 2229
Fragments arranged by size:
2229 1740 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5611
PciI A'CATG_T
Cuts at: 4510 4510
Size: 5611
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2734 1108
Size: 1626 3985
PfoI T'CCnGG_A
Cuts at: 2685 2685
Size: 5611
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 5193 5225 3680
Size: 32 1481 32 4066
Fragments arranged by size:
4066 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5611
PsiI TTA'TAA
Cuts at: 3181 3181
Size: 5611
PspOMI G'GGCC_C
Cuts at: 2058 2058
Size: 5611
PstI C_TGCA'G
Cuts at: 830 2625 2661 2877 2963 830
Size: 1795 36 216 86 3478
Fragments arranged by size:
3478 1795 216 86 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
PvuII CAG'CTG
Cuts at: 2545 2626 2761 2545
Size: 81 135 5395
Fragments arranged by size:
5395 135 81
RsrII CG'GwC_CG
Cuts at: 2299 2299
Size: 5611
SacII CC_GC'GG
Cuts at: 2281 2281
Size: 5611
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SapI GCTCTTCn'nnn_
Cuts at: 2642 2642
Size: 5611
SbfI CC_TGCA'GG
Cuts at: 2661 2963 2661
Size: 302 5309
ScaI AGT'ACT
Cuts at: 3859 5571 3859
Size: 1712 3899
SexAI A'CCwGG_T
Cuts at: 2421 2676 2421
Size: 255 5356
SfcI C'TryA_G
Cuts at: 826 1045 2621 2657 2873 2959 4094 4775 4966
Size: 219 1576 36 216 86 1135 681 191
Cuts at: 4966 826
Size: 1471
Fragments arranged by size:
1576 1471 1135 681 219 216 191 86
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 2644 1700
Size: 944 4667
SmaI CCC'GGG
Cuts at: 1872 2070 2307 2938 1872
Size: 198 237 631 4545
Fragments arranged by size:
4545 631 237 198
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3715 4616 4878 820
Size: 197 926 1772 901 262 1553
Fragments arranged by size:
1772 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3402 493
Size: 2909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5611
SphI G_CATG'C
Cuts at: 1132 2969 1132
Size: 1837 3774
SspI AAT'ATT
Cuts at: 5 52 3535 5
Size: 47 3483 2081
Fragments arranged by size:
3483 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5611
StyI C'CwwG_G
Cuts at: 513 1065 2004 2766 3050 513
Size: 552 939 762 284 3074
Fragments arranged by size:
3074 939 762 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3638 3655 3808 3993 4438 325
Size: 1255 2058 17 153 185 445 1498
Fragments arranged by size:
2058 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2880 3857 5569
Size: 274 80 33 51 1443 903 977 1712
Cuts at: 5569 96
Size: 138
Fragments arranged by size:
1712 1443 977 903 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5571 5603 5571
Size: 32 5579
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5611
XbaI T'CTAG_A
Cuts at: 2947 2947
Size: 5611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2425 2011
Size: 414 5197
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5611
XmaI C'CCGG_G
Cuts at: 1870 2068 2305 2936 1870
Size: 198 237 631 4545
Fragments arranged by size:
4545 631 237 198
XmnI GAAnn'nnTTC
Cuts at: 3740 3740
Size: 5611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3419 276
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspXI PsrI SacI SanDI SfiI SfoI SrfI
SwaI