Restriction map for
pFN21AE1005
(Circular) (Six-base) MAPSORT of: pFN21AE1005.seq Check: 189 from: 1 to: 5263
pFN21AE1005 5263 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2360 2360
Size: 5263
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3073 278
Size: 53 83 186 936 1537 2468
Fragments arranged by size:
2468 1537 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2232 2606 1937
Size: 295 374 4594
Fragments arranged by size:
4594 374 295
Acc65I G'GTAC_C
Cuts at: 2584 2584
Size: 5263
AclI AA'CG_TT
Cuts at: 1563 3390 3763 1563
Size: 1827 373 3063
Fragments arranged by size:
3063 1827 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3326 4710 4978 1632
Size: 1694 1384 268 1917
Fragments arranged by size:
1917 1694 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5066
AflIII A'CryG_T
Cuts at: 1203 4162 5139 1203
Size: 2959 977 1327
Fragments arranged by size:
2959 1327 977
AgeI A'CCGG_T
Cuts at: 4094 4094
Size: 5263
AhdI GACnn_n'nnGTC
Cuts at: 3992 3992
Size: 5263
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5263
AlwNI CAG_nnn'CTG
Cuts at: 3006 4578 3006
Size: 1572 3691
ApaLI G'TGCA_C
Cuts at: 3320 4476 3320
Size: 1156 4107
ApoI r'AATT_y
Cuts at: 1493 2501 2572 2798 2950 5059 1493
Size: 1008 71 226 152 2109 1697
Fragments arranged by size:
2109 1697 1008 226 152 71
AseI AT'TA_AT
Cuts at: 160 1032 3817 160
Size: 872 2785 1606
Fragments arranged by size:
2785 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5263
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 2204 2399 2588 1870
Size: 73 88 173 195 189 4545
Fragments arranged by size:
4545 195 189 173 88 73
BamHI G'GATC_C
Cuts at: 2250 2593 2960 2250
Size: 343 367 4553
Fragments arranged by size:
4553 367 343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2584 4039 618
Size: 325 217 627 288 509 1455 1842
Fragments arranged by size:
1842 1455 627 509 325 288 217
BanII G_rGCy'C
Cuts at: 1391 2582 1391
Size: 1191 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2379 2418 928
Size: 971 480 39 3773
Fragments arranged by size:
3773 971 480 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3454 3488 1903
Size: 34 50 34 1433 34 3678
Fragments arranged by size:
3678 1433 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3155 4365 3155
Size: 1210 4053
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5263
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5263
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 2339 3874
Size: 107 122 71 631 605 355 312 1535
Cuts at: 3874 136
Size: 1525
Fragments arranged by size:
1535 1525 631 605 355 312 122 107
71
BglII A'GATC_T
Cuts at: 5258 5258
Size: 5263
BlpI GC'TnA_GC
Cuts at: 2680 2680
Size: 5263
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2102 3324 4480 1790
Size: 288 24 1222 1156 2573
Fragments arranged by size:
2573 1222 1156 288 24
BmgBI CAC'GTC
Cuts at: 1318 2212 1318
Size: 894 4369
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2249 2994 3952
Size: 416 53 83 399 24 832 745 958
Cuts at: 3952 442
Size: 1753
Fragments arranged by size:
1753 958 832 745 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2426 3923 1239
Size: 1187 1497 2579
Fragments arranged by size:
2579 1497 1187
Bpu10I CC'TnA_GC
Cuts at: 2239 2239
Size: 5263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3388 4253 4551 3388
Size: 865 298 4100
Fragments arranged by size:
4100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3926 882
Size: 617 2427 2219
Fragments arranged by size:
2427 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2536 3054 493
Size: 711 1332 518 2702
Fragments arranged by size:
2702 1332 711 518
BsaBI GATnn'nnATC
Cuts at: 5257 5257
Size: 5263
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3070 3452 4087 275
Size: 53 83 186 936 1537 382 635 1451
Fragments arranged by size:
1537 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2482 3696 4094 4368 4515 4926 2482
Size: 1214 398 274 147 411 2819
Fragments arranged by size:
2819 1214 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5038
Fragments arranged by size:
5038 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2385 2417 2429 1186
Size: 1006 193 32 12 4020
Fragments arranged by size:
4020 1006 193 32 12
BseYI C'CCAG_C
Cuts at: 2128 2295 2458 4466 4962 2128
Size: 167 163 2008 496 2429
Fragments arranged by size:
2429 2008 496 167 163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4690
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2713 3474 4502 1540
Size: 275 186 712 761 1028 2301
Fragments arranged by size:
2301 1028 761 712 275 186
BsiHKAI G_wGCw'C
Cuts at: 3324 3409 4480 3324
Size: 85 1071 4107
Fragments arranged by size:
4107 1071 85
BsmI GAATG_Cn'
Cuts at: 2774 2867 2774
Size: 93 5170
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2102 2582 3324 3409 4480 1391
Size: 399 288 24 480 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 399 288 85 24
BspEI T'CCGG_A
Cuts at: 4926 4926
Size: 5263
BspHI T'CATG_A
Cuts at: 3152 4882 3152
Size: 1730 3533
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2360 2618 844
Size: 1516 258 3489
Fragments arranged by size:
3489 1516 258
BsrBI CCG'CTC
Cuts at: 1394 1823 2200 2238 2498 3150 1394
Size: 429 377 38 260 652 3507
Fragments arranged by size:
3507 652 429 377 260 38
BsrDI GCAATG_nn'
Cuts at: 65 1248 3758 3932 65
Size: 1183 2510 174 1396
Fragments arranged by size:
2510 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3907 4094 1700
Size: 110 2097 187 2869
Fragments arranged by size:
2869 2097 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5263
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5263
BssSI C'ACGA_G
Cuts at: 3323 4335 3323
Size: 1012 4251
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5263
BstBI TT'CG_AA
Cuts at: 4067 4067
Size: 5263
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5263
BstYI r'GATC_y
Cuts at: 1972 2250 2383 2593 2960 3345 3362 4814 4900
Size: 278 133 210 367 385 17 1452 86
Cuts at: 4900 4912 5258 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 278 210 133
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2025 2302 513
Size: 552 960 277 3474
Fragments arranged by size:
3474 960 552 277
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4524
Fragments arranged by size:
4524 683 56
BtsI GCAGTG_nn'
Cuts at: 2779 3573 3593 2779
Size: 794 20 4449
Fragments arranged by size:
4449 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5263
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5228
DraI TTT'AAA
Cuts at: 2567 2567
Size: 5263
DraIII CAC_nnn'GTG
Cuts at: 2082 2308 2082
Size: 226 5037
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4270 809
Size: 507 2954 1802
Fragments arranged by size:
2954 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2542 2710
Size: 54 1425 50 275 18 186 526 168
Cuts at: 2710 3599 5228 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 526 275 186 168 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2710 1537
Size: 275 898 4090
Fragments arranged by size:
4090 898 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3193 1353
Size: 1840 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3850 4224 4370 387
Size: 1064 138 2261 374 146 1280
Fragments arranged by size:
2261 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2426 3326 3923 4710 4978 1239
Size: 393 794 900 597 787 268 1524
Fragments arranged by size:
1524 900 794 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2441 2441
Size: 5263
EcoO109I rG'GnC_Cy
Cuts at: 2322 2322
Size: 5263
EcoRI G'AATT_C
Cuts at: 2501 2572 2950 2501
Size: 71 378 4814
Fragments arranged by size:
4814 378 71
FspI TGC'GCA
Cuts at: 3769 3769
Size: 5263
HaeII r_GCGC'y
Cuts at: 1678 2129 2302 4410 1678
Size: 451 173 2108 2531
Fragments arranged by size:
2531 2108 451 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3904 3936 3978 4010 1373
Size: 32 135 32 2332 32 42 32 2626
Fragments arranged by size:
2626 2332 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2607 2853 669
Size: 988 281 669 246 3079
Fragments arranged by size:
3079 988 669 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5263
HpaI GTT'AAC
Cuts at: 2853 2853
Size: 5263
KpnI G_GTAC'C
Cuts at: 2588 2588
Size: 5263
MfeI C'AATT_G
Cuts at: 2862 2862
Size: 5263
MluI A'CGCG_T
Cuts at: 5139 5139
Size: 5263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4377 4561 1305
Size: 3072 184 2007
Fragments arranged by size:
3072 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2544 10
Size: 54 1425 343 712 2729
Fragments arranged by size:
2729 1425 712 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2361 3221 3580 3739 518
Size: 648 1195 860 359 159 2042
Fragments arranged by size:
2042 1195 860 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2679 3356 4504 4749 1606
Size: 642 431 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 642 431 245
NcoI C'CATG_G
Cuts at: 513 1065 2025 513
Size: 552 960 3751
Fragments arranged by size:
3751 960 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5263
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5263
NotI GC'GGCC_GC
Cuts at: 2710 2710
Size: 5263
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5263
NspI r_CATG'y
Cuts at: 1132 1229 2012 2422 2621 4166 1132
Size: 97 783 410 199 1545 2229
Fragments arranged by size:
2229 1545 783 410 199 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5263
PciI A'CATG_T
Cuts at: 4162 4162
Size: 5263
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2261 2308 1108
Size: 1153 47 4063
Fragments arranged by size:
4063 1153 47
PfoI T'CCnGG_A
Cuts at: 2442 2442
Size: 5263
PmeI GTTT'AAAC
Cuts at: 2567 2567
Size: 5263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 4845 4877 3332
Size: 32 1481 32 3718
Fragments arranged by size:
3718 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5263
PsiI TTA'TAA
Cuts at: 2833 2833
Size: 5263
PstI C_TGCA'G
Cuts at: 830 2064 2615 830
Size: 1234 551 3478
Fragments arranged by size:
3478 1234 551
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5263
SalI G'TCGA_C
Cuts at: 1936 2605 1936
Size: 669 4594
SbfI CC_TGCA'GG
Cuts at: 2615 2615
Size: 5263
ScaI AGT'ACT
Cuts at: 3511 5223 3511
Size: 1712 3551
SexAI A'CCwGG_T
Cuts at: 2078 2078
Size: 5263
SfcI C'TryA_G
Cuts at: 826 1045 2060 2611 3746 4427 4618 826
Size: 219 1015 551 1135 681 191 1471
Fragments arranged by size:
1471 1135 1015 681 551 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5263
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5263
SmaI CCC'GGG
Cuts at: 1872 2033 2206 2590 1872
Size: 161 173 384 4545
Fragments arranged by size:
4545 384 173 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3367 4268 4530 820
Size: 197 926 1424 901 262 1553
Fragments arranged by size:
1553 1424 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3054 493
Size: 2561 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5263
SphI G_CATG'C
Cuts at: 1132 2621 1132
Size: 1489 3774
SspI AAT'ATT
Cuts at: 5 52 3187 5
Size: 47 3135 2081
Fragments arranged by size:
3135 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5263
StyI C'CwwG_G
Cuts at: 513 1065 2025 2702 513
Size: 552 960 677 3074
Fragments arranged by size:
3074 960 677 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3290 3307 3460 3645 4090 325
Size: 1255 1710 17 153 185 445 1498
Fragments arranged by size:
1710 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3509 5221 96
Size: 274 80 33 51 1443 1532 1712 138
Fragments arranged by size:
1712 1532 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5223 5255 5223
Size: 32 5231
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5263
XbaI T'CTAG_A
Cuts at: 2599 2599
Size: 5263
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5263
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5263
XmaI C'CCGG_G
Cuts at: 1870 2031 2204 2588 1870
Size: 161 173 384 4545
Fragments arranged by size:
4545 384 173 161
XmnI GAAnn'nnTTC
Cuts at: 3392 3392
Size: 5263
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3071 276
Size: 53 83 186 936 1537 2468
Fragments arranged by size:
2468 1537 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI