Restriction map for
pFN21AE1001
(Circular) (Six-base) MAPSORT of: pFN21AE1001.seq Check: 5195 from: 1 to: 5104
pFN21AE1001 5104 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2914 278
Size: 53 83 186 936 1378 2468
Fragments arranged by size:
2468 1378 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2447 1937
Size: 510 4594
Acc65I G'GTAC_C
Cuts at: 2037 2425 2037
Size: 388 4716
AclI AA'CG_TT
Cuts at: 1563 3231 3604 1563
Size: 1668 373 3063
Fragments arranged by size:
3063 1668 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 2357 3167 4551 4819 1632
Size: 555 170 810 1384 268 1917
Fragments arranged by size:
1917 1384 810 555 268 170
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4907
AflIII A'CryG_T
Cuts at: 1203 2193 4003 4980 1203
Size: 990 1810 977 1327
Fragments arranged by size:
1810 1327 990 977
AgeI A'CCGG_T
Cuts at: 3935 3935
Size: 5104
AhdI GACnn_n'nnGTC
Cuts at: 3833 3833
Size: 5104
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5104
AlwNI CAG_nnn'CTG
Cuts at: 2847 4419 2847
Size: 1572 3532
ApaI G_GGCC'C
Cuts at: 2143 2143
Size: 5104
ApaLI G'TGCA_C
Cuts at: 3161 4317 3161
Size: 1156 3948
ApoI r'AATT_y
Cuts at: 1493 2413 2639 2791 4900 1493
Size: 920 226 152 2109 1697
Fragments arranged by size:
2109 1697 920 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3658 160
Size: 872 2626 1606
Fragments arranged by size:
2626 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5104
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2429 1870
Size: 73 72 414 4545
Fragments arranged by size:
4545 414 73 72
BamHI G'GATC_C
Cuts at: 2434 2801 2434
Size: 367 4737
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 2425 3880 618
Size: 325 217 627 250 388 1455 1842
Fragments arranged by size:
1842 1455 627 388 325 250 217
BanII G_rGCy'C
Cuts at: 1391 2061 2143 2423 1391
Size: 670 82 280 4072
Fragments arranged by size:
4072 670 280 82
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3295 3329 1903
Size: 34 50 34 1274 34 3678
Fragments arranged by size:
3678 1274 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2996 4206 2996
Size: 1210 3894
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5104
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3715 136
Size: 107 122 71 631 605 2043 1525
Fragments arranged by size:
2043 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5099 5099
Size: 5104
BlpI GC'TnA_GC
Cuts at: 2521 2521
Size: 5104
Bme1580I G_kGCm'C
Cuts at: 1790 2143 3165 4321 1790
Size: 353 1022 1156 2573
Fragments arranged by size:
2573 1156 1022 353
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5104
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2835 3793 442
Size: 416 53 83 399 24 1418 958 1753
Fragments arranged by size:
1753 1418 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3764 1239
Size: 2525 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 3229 4094 4392 2369
Size: 860 865 298 3081
Fragments arranged by size:
3081 865 860 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3767 882
Size: 617 2268 2219
Fragments arranged by size:
2268 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2895 493
Size: 711 1691 2702
Fragments arranged by size:
2702 1691 711
BsaBI GATnn'nnATC
Cuts at: 2247 5098 2247
Size: 2851 2253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2911 3293 3928 275
Size: 53 83 186 936 1378 382 635 1451
Fragments arranged by size:
1451 1378 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2329 3537 3935 4209 4356 4767 2329
Size: 1208 398 274 147 411 2666
Fragments arranged by size:
2666 1208 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4879
Fragments arranged by size:
4879 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2081 1186
Size: 883 12 4209
Fragments arranged by size:
4209 883 12
BseYI C'CCAG_C
Cuts at: 4307 4803 4307
Size: 496 4608
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4531
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2554 3315 4343 1540
Size: 275 186 553 761 1028 2301
Fragments arranged by size:
2301 1028 761 553 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 3165 3250 4321 2061
Size: 1104 85 1071 2844
Fragments arranged by size:
2844 1104 1071 85
BsmI GAATG_Cn'
Cuts at: 2615 2708 2615
Size: 93 5011
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2143 2423 3165 3250 4321 1391
Size: 399 271 82 280 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 280 271 85 82
BspEI T'CCGG_A
Cuts at: 4767 4767
Size: 5104
BspHI T'CATG_A
Cuts at: 2993 4723 2993
Size: 1730 3374
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2459 844
Size: 1615 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2991 1394
Size: 429 1168 3507
Fragments arranged by size:
3507 1168 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3599 3773 65
Size: 1183 2351 174 1396
Fragments arranged by size:
2351 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3748 3935 1700
Size: 110 1938 187 2869
Fragments arranged by size:
2869 1938 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5104
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5104
BssSI C'ACGA_G
Cuts at: 3164 4176 3164
Size: 1012 4092
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5104
BstBI TT'CG_AA
Cuts at: 3908 3908
Size: 5104
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5104
BstYI r'GATC_y
Cuts at: 1972 2152 2434 2801 3186 3203 4655 4741 4753
Size: 180 282 367 385 17 1452 86 12
Cuts at: 4753 5099 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 282 180 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2135 2135
Size: 5104
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4365
Fragments arranged by size:
4365 683 56
BtsI GCAGTG_nn'
Cuts at: 2620 3414 3434 2620
Size: 794 20 4290
Fragments arranged by size:
4290 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5069
DraI TTT'AAA
Cuts at: 2408 2408
Size: 5104
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4111 809
Size: 507 2795 1802
Fragments arranged by size:
2795 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2551 3440 5069
Size: 54 1425 50 275 18 721 889 1629
Cuts at: 5069 8
Size: 43
Fragments arranged by size:
1629 1425 889 721 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2551 1537
Size: 275 739 4090
Fragments arranged by size:
4090 739 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3034 1353
Size: 1681 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 3691 4065 4211 387
Size: 1064 138 504 1598 374 146 1280
Fragments arranged by size:
1598 1280 1064 504 374 146 138
EcoICRI GAG'CTC
Cuts at: 2059 2059
Size: 5104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2357 3167 3764 4551 4819 1239
Size: 393 555 170 810 597 787 268 1524
Fragments arranged by size:
1524 810 787 597 555 393 268 170
EcoNI CCTnn'n_nnAGG
Cuts at: 2147 2312 2363 2147
Size: 165 51 4888
Fragments arranged by size:
4888 165 51
EcoO109I rG'GnC_Cy
Cuts at: 2131 2139 2140 2131
Size: 8 1 5095
Fragments arranged by size:
5095 8 1
EcoRI G'AATT_C
Cuts at: 2413 2791 2413
Size: 378 4726
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 5104
FspI TGC'GCA
Cuts at: 3610 3610
Size: 5104
HaeII r_GCGC'y
Cuts at: 1678 4251 1678
Size: 2573 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3745 3777 3819 3851 1373
Size: 32 135 32 2173 32 42 32 2626
Fragments arranged by size:
2626 2173 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2302 2448 2694 669
Size: 988 281 364 146 246 3079
Fragments arranged by size:
3079 988 364 281 246 146
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5104
HpaI GTT'AAC
Cuts at: 2694 2694
Size: 5104
KpnI G_GTAC'C
Cuts at: 2041 2429 2041
Size: 388 4716
MfeI C'AATT_G
Cuts at: 2703 2703
Size: 5104
MluI A'CGCG_T
Cuts at: 4980 4980
Size: 5104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4218 4402 1305
Size: 2913 184 2007
Fragments arranged by size:
2913 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3282
Fragments arranged by size:
3282 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3062 3421 3580 518
Size: 648 1896 359 159 2042
Fragments arranged by size:
2042 1896 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2520 3197 4345 4590 1606
Size: 914 677 1148 245 2120
Fragments arranged by size:
2120 1148 914 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5104
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5104
NotI GC'GGCC_GC
Cuts at: 2551 2551
Size: 5104
NspI r_CATG'y
Cuts at: 1132 1229 2197 2462 4007 1132
Size: 97 968 265 1545 2229
Fragments arranged by size:
2229 1545 968 265 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5104
PciI A'CATG_T
Cuts at: 2193 4003 2193
Size: 1810 3294
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5104
PmeI GTTT'AAAC
Cuts at: 2408 2408
Size: 5104
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 4686 4718 3173
Size: 32 1481 32 3559
Fragments arranged by size:
3559 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5104
PsiI TTA'TAA
Cuts at: 2674 2674
Size: 5104
PspOMI G'GGCC_C
Cuts at: 2139 2139
Size: 5104
PstI C_TGCA'G
Cuts at: 830 2148 2456 830
Size: 1318 308 3478
Fragments arranged by size:
3478 1318 308
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5104
SacI G_AGCT'C
Cuts at: 2061 2061
Size: 5104
SalI G'TCGA_C
Cuts at: 1936 2446 1936
Size: 510 4594
SbfI CC_TGCA'GG
Cuts at: 2456 2456
Size: 5104
ScaI AGT'ACT
Cuts at: 3352 5064 3352
Size: 1712 3392
SfcI C'TryA_G
Cuts at: 826 1045 2144 2452 3587 4268 4459 826
Size: 219 1099 308 1135 681 191 1471
Fragments arranged by size:
1471 1135 1099 681 308 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5104
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5104
SmaI CCC'GGG
Cuts at: 1872 2431 1872
Size: 559 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 3208 4109 4371 820
Size: 197 926 441 824 901 262 1553
Fragments arranged by size:
1553 926 901 824 441 262 197
SnaBI TAC'GTA
Cuts at: 493 2895 493
Size: 2402 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5104
SphI G_CATG'C
Cuts at: 1132 2462 1132
Size: 1330 3774
SspI AAT'ATT
Cuts at: 5 52 3028 5
Size: 47 2976 2081
Fragments arranged by size:
2976 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5104
StyI C'CwwG_G
Cuts at: 513 1065 2320 2543 513
Size: 552 1255 223 3074
Fragments arranged by size:
3074 1255 552 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3131 3148 3301 3486 3931 325
Size: 1255 1551 17 153 185 445 1498
Fragments arranged by size:
1551 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3350 5062 96
Size: 274 80 33 51 1443 1373 1712 138
Fragments arranged by size:
1712 1443 1373 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5064 5096 5064
Size: 32 5072
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5104
XbaI T'CTAG_A
Cuts at: 2440 2440
Size: 5104
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5104
XmaI C'CCGG_G
Cuts at: 1870 2429 1870
Size: 559 4545
XmnI GAAnn'nnTTC
Cuts at: 3233 3233
Size: 5104
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2912 276
Size: 53 83 186 936 1378 2468
Fragments arranged by size:
2468 1378 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI