Restriction map for
pFN21AE0999
(Circular) (Six-base) MAPSORT of: pFN21AE0999.seq Check: 6668 from: 1 to: 6274
pFN21AE0999 6274 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2544 2544
Size: 6274
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4084 278
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3617 1937
Size: 1680 4594
Acc65I G'GTAC_C
Cuts at: 3595 3595
Size: 6274
AclI AA'CG_TT
Cuts at: 1563 4401 4774 1563
Size: 2838 373 3063
Fragments arranged by size:
3063 2838 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 2355 3194 4337 5721 5989 1632
Size: 708 15 839 1143 1384 268 1917
Fragments arranged by size:
1917 1384 1143 839 708 268 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6077
AflIII A'CryG_T
Cuts at: 1203 2473 5173 6150 1203
Size: 1270 2700 977 1327
Fragments arranged by size:
2700 1327 1270 977
AgeI A'CCGG_T
Cuts at: 5105 5105
Size: 6274
AhdI GACnn_n'nnGTC
Cuts at: 5003 5003
Size: 6274
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6274
AlwNI CAG_nnn'CTG
Cuts at: 2588 4017 5589 2588
Size: 1429 1572 3273
Fragments arranged by size:
3273 1572 1429
ApaLI G'TGCA_C
Cuts at: 2864 4331 5487 2864
Size: 1467 1156 3651
Fragments arranged by size:
3651 1467 1156
ApoI r'AATT_y
Cuts at: 1493 2849 3583 3809 3961 6070 1493
Size: 1356 734 226 152 2109 1697
Fragments arranged by size:
2109 1697 1356 734 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4828 160
Size: 872 3796 1606
Fragments arranged by size:
3796 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 2121 3599 1870
Size: 73 164 14 1478 4545
Fragments arranged by size:
4545 1478 164 73 14
BamHI G'GATC_C
Cuts at: 3539 3604 3971 3539
Size: 65 367 5842
Fragments arranged by size:
5842 367 65
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3489 3595 5050 618
Size: 325 217 627 1702 106 1455 1842
Fragments arranged by size:
1842 1702 1455 627 325 217 106
BanII G_rGCy'C
Cuts at: 1391 3593 1391
Size: 2202 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2221 2536 928
Size: 971 322 315 4666
Fragments arranged by size:
4666 971 322 315
BbvCI CC'TCA_GC
Cuts at: 3423 3423
Size: 6274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2530 2564 4465 4499 1903
Size: 34 593 34 1901 34 3678
Fragments arranged by size:
3678 1901 593 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4166 5376 4166
Size: 1210 5064
BclI T'GATC_A
Cuts at: 1548 2923 1548
Size: 1375 4899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4885 136
Size: 107 122 71 631 605 3213 1525
Fragments arranged by size:
3213 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2773 6269 2773
Size: 3496 2778
BlpI GC'TnA_GC
Cuts at: 3691 3691
Size: 6274
Bme1580I G_kGCm'C
Cuts at: 1790 2582 2868 4335 5491 1790
Size: 792 286 1467 1156 2573
Fragments arranged by size:
2573 1467 1156 792 286
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6274
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4005 4963 442
Size: 416 53 83 399 24 2588 958 1753
Fragments arranged by size:
2588 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6274
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 6274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2705 3022 3306 3521 4934 1239
Size: 1466 317 284 215 1413 2579
Fragments arranged by size:
2579 1466 1413 317 284 215
Bpu10I CC'TnA_GC
Cuts at: 3423 3423
Size: 6274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4399 5264 5562 4399
Size: 865 298 5111
Fragments arranged by size:
5111 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3432 4937 882
Size: 617 1933 1505 2219
Fragments arranged by size:
2219 1933 1505 617
BsaAI yAC'GTr
Cuts at: 493 1204 2474 4065 493
Size: 711 1270 1591 2702
Fragments arranged by size:
2702 1591 1270 711
BsaBI GATnn'nnATC
Cuts at: 6268 6268
Size: 6274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4081 4463 5098 275
Size: 53 83 186 936 2548 382 635 1451
Fragments arranged by size:
2548 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4707 5105 5379 5526 5937 4707
Size: 398 274 147 411 5044
Fragments arranged by size:
5044 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6049
Fragments arranged by size:
6049 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2616 1186
Size: 1430 4844
BseYI C'CCAG_C
Cuts at: 2425 3134 5477 5973 2425
Size: 709 2343 496 2726
Fragments arranged by size:
2726 2343 709 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 3184 3424 1163
Size: 573 303 1145 240 4013
Fragments arranged by size:
4013 1145 573 303 240
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3724 4485 5513 1540
Size: 275 186 1723 761 1028 2301
Fragments arranged by size:
2301 1723 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2108 2314 2868 4335 4420 5491 2108
Size: 206 554 1467 85 1071 2891
Fragments arranged by size:
2891 1467 1071 554 206 85
BsmI GAATG_Cn'
Cuts at: 3785 3878 3785
Size: 93 6181
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2314 2582 2868 3593 4335 4420
Size: 399 318 206 268 286 725 742 85
Cuts at: 4420 5491 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 725 399 318 286 268
206 85
BspEI T'CCGG_A
Cuts at: 5937 5937
Size: 6274
BspHI T'CATG_A
Cuts at: 4163 5893 4163
Size: 1730 4544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2542 2544 3629 844
Size: 1698 2 1085 3489
Fragments arranged by size:
3489 1698 1085 2
BsrBI CCG'CTC
Cuts at: 1394 1823 4161 1394
Size: 429 2338 3507
Fragments arranged by size:
3507 2338 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3351 3494 4769 4943 65
Size: 1183 2103 143 1275 174 1396
Fragments arranged by size:
2103 1396 1275 1183 174 143
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 4918 5105 1700
Size: 110 271 2837 187 2869
Fragments arranged by size:
2869 2837 271 187 110
BsrGI T'GTAC_A
Cuts at: 96 3291 96
Size: 3195 3079
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6274
BssSI C'ACGA_G
Cuts at: 4334 5346 4334
Size: 1012 5262
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3496 794
Size: 2702 3572
BstBI TT'CG_AA
Cuts at: 5078 5078
Size: 6274
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6274
BstYI r'GATC_y
Cuts at: 1972 2773 3202 3539 3604 3971 4356 4373 5825
Size: 801 429 337 65 367 385 17 1452
Cuts at: 5825 5911 5923 6269 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 801 429 385 367 346 337
86 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5722
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3194 487
Size: 683 56 1968 3567
Fragments arranged by size:
3567 1968 683 56
BtsI GCAGTG_nn'
Cuts at: 3790 4584 4604 3790
Size: 794 20 5460
Fragments arranged by size:
5460 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2680 2715 529
Size: 35 2116 35 4088
Fragments arranged by size:
4088 2116 35 35
DraI TTT'AAA
Cuts at: 2448 2880 3578 2448
Size: 432 698 5144
Fragments arranged by size:
5144 698 432
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5281 809
Size: 507 3965 1802
Fragments arranged by size:
3965 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 2246 3511
Size: 54 1425 50 275 18 253 163 1265
Cuts at: 3511 3721 4610 6239 8
Size: 210 889 1629 43
Fragments arranged by size:
1629 1425 1265 889 275 253 210 163
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3721 1537
Size: 275 1909 4090
Fragments arranged by size:
4090 1909 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2331 4204 1353
Size: 978 1873 3423
Fragments arranged by size:
3423 1873 978
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2157 4861 5235 5381 387
Size: 1064 138 568 2704 374 146 1280
Fragments arranged by size:
2704 1280 1064 568 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2340 2355 2705 3022 3194 3306 3521
Size: 393 708 15 350 317 172 112 215
Cuts at: 3521 4337 4934 5721 5989 1239
Size: 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 708 597 393 350 317
268 215 172 112 15
EcoRI G'AATT_C
Cuts at: 3583 3961 3583
Size: 378 5896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3023 3023
Size: 6274
FspI TGC'GCA
Cuts at: 4780 4780
Size: 6274
HaeII r_GCGC'y
Cuts at: 1678 5421 1678
Size: 3743 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2486 2518 2866 2898 4915
Size: 32 135 32 914 32 348 32 2017
Cuts at: 4915 4947 4989 5021 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2017 914 348 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2662 3618 3864 669
Size: 988 281 724 956 246 3079
Fragments arranged by size:
3079 988 956 724 281 246
HindIII A'AGCT_T
Cuts at: 748 2766 748
Size: 2018 4256
HpaI GTT'AAC
Cuts at: 3864 3864
Size: 6274
KpnI G_GTAC'C
Cuts at: 3599 3599
Size: 6274
MfeI C'AATT_G
Cuts at: 3873 3873
Size: 6274
MluI A'CGCG_T
Cuts at: 6150 6150
Size: 6274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 5388 5572 1305
Size: 996 3087 184 2007
Fragments arranged by size:
3087 2007 996 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2248 10
Size: 54 1425 343 416 4036
Fragments arranged by size:
4036 1425 416 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2472 2952 3398 3447 4232 4591 4750
Size: 648 1306 480 446 49 785 359 159
Cuts at: 4750 518
Size: 2042
Fragments arranged by size:
2042 1306 785 648 480 446 359 159
49
MspA1I CmG'CkG
Cuts at: 1606 3690 4367 5515 5760 1606
Size: 2084 677 1148 245 2120
Fragments arranged by size:
2120 2084 1148 677 245
NaeI GCC'GGC
Cuts at: 2083 2083
Size: 6274
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5722
NdeI CA'TA_TG
Cuts at: 387 2920 387
Size: 2533 3741
NgoMIV G'CCGG_C
Cuts at: 2081 2081
Size: 6274
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6274
NotI GC'GGCC_GC
Cuts at: 3721 3721
Size: 6274
NspI r_CATG'y
Cuts at: 1132 1229 2471 3632 5177 1132
Size: 97 1242 1161 1545 2229
Fragments arranged by size:
2229 1545 1242 1161 97
PacI TTA_AT'TAA
Cuts at: 2463 2463
Size: 6274
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6274
PciI A'CATG_T
Cuts at: 5173 5173
Size: 6274
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3054 1108
Size: 1946 4328
PmeI GTTT'AAAC
Cuts at: 3578 3578
Size: 6274
PmlI CAC'GTG
Cuts at: 2474 2474
Size: 6274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 5856 5888 4343
Size: 32 1481 32 4729
Fragments arranged by size:
4729 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6274
PsiI TTA'TAA
Cuts at: 3844 3844
Size: 6274
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2577 2609 2577
Size: 32 6242
PstI C_TGCA'G
Cuts at: 830 3626 830
Size: 2796 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6274
SalI G'TCGA_C
Cuts at: 1936 3616 1936
Size: 1680 4594
SbfI CC_TGCA'GG
Cuts at: 3626 3626
Size: 6274
ScaI AGT'ACT
Cuts at: 4522 6234 4522
Size: 1712 4562
SexAI A'CCwGG_T
Cuts at: 3336 3336
Size: 6274
SfcI C'TryA_G
Cuts at: 826 1045 2913 3622 4757 5438 5629 826
Size: 219 1868 709 1135 681 191 1471
Fragments arranged by size:
1868 1471 1135 709 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6274
SmaI CCC'GGG
Cuts at: 1872 3601 1872
Size: 1729 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4378 5279 5541 820
Size: 197 926 2435 901 262 1553
Fragments arranged by size:
2435 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4065 493
Size: 3572 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6274
SphI G_CATG'C
Cuts at: 1132 3632 1132
Size: 2500 3774
SspI AAT'ATT
Cuts at: 5 52 4198 5
Size: 47 4146 2081
Fragments arranged by size:
4146 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6274
StyI C'CwwG_G
Cuts at: 513 1065 3713 513
Size: 552 2648 3074
Fragments arranged by size:
3074 2648 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3086 3507 4301 4318 4471 4656 5101
Size: 1255 1506 421 794 17 153 185 445
Cuts at: 5101 325
Size: 1498
Fragments arranged by size:
1506 1498 1255 794 445 421 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3291 4520 6232
Size: 274 80 33 51 1443 1314 1229 1712
Cuts at: 6232 96
Size: 138
Fragments arranged by size:
1712 1443 1314 1229 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2275 2307 6234 6266 2275
Size: 32 3927 32 2283
Fragments arranged by size:
3927 2283 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3338 1316
Size: 2022 4252
XbaI T'CTAG_A
Cuts at: 2978 3610 2978
Size: 632 5642
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3358 3483 3358
Size: 125 6149
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6274
XmaI C'CCGG_G
Cuts at: 1870 3599 1870
Size: 1729 4545
XmnI GAAnn'nnTTC
Cuts at: 2901 3003 4403 2901
Size: 102 1400 4772
Fragments arranged by size:
4772 1400 102
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4082 276
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI
PfoI PpuMI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI