Restriction map for
pFN21AE0994
(Circular) (Six-base) MAPSORT of: pFN21AE0994.seq Check: 3051 from: 1 to: 6646
pFN21AE0994 6646 bp
With 182 enzymes: SgfI *
March 28, 2012 12:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2091 3022 2091
Size: 931 5715
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4456 278
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3989 1937
Size: 2052 4594
Acc65I G'GTAC_C
Cuts at: 3967 3967
Size: 6646
AclI AA'CG_TT
Cuts at: 1563 4773 5146 1563
Size: 3210 373 3063
Fragments arranged by size:
3210 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2271 2951 3290 3893 4709 6093 6361 1632
Size: 639 680 339 603 816 1384 268 1917
Fragments arranged by size:
1917 1384 816 680 639 603 339 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6449
AflIII A'CryG_T
Cuts at: 1203 3325 5545 6522 1203
Size: 2122 2220 977 1327
Fragments arranged by size:
2220 2122 1327 977
AgeI A'CCGG_T
Cuts at: 5477 5477
Size: 6646
AhdI GACnn_n'nnGTC
Cuts at: 5375 5375
Size: 6646
AleI CACnn'nnGTG
Cuts at: 1166 2144 1166
Size: 978 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6646
AlwNI CAG_nnn'CTG
Cuts at: 2354 2845 4389 5961 2354
Size: 491 1544 1572 3039
Fragments arranged by size:
3039 1572 1544 491
ApaI G_GGCC'C
Cuts at: 2131 2348 2969 2131
Size: 217 621 5808
Fragments arranged by size:
5808 621 217
ApaLI G'TGCA_C
Cuts at: 3035 4703 5859 3035
Size: 1668 1156 3822
Fragments arranged by size:
3822 1668 1156
ApoI r'AATT_y
Cuts at: 1493 3955 4181 4333 6442 1493
Size: 2462 226 152 2109 1697
Fragments arranged by size:
2462 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5200 160
Size: 872 4168 1606
Fragments arranged by size:
4168 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 2607 3176 3812 3971 1870
Size: 73 624 40 569 636 159 4545
Fragments arranged by size:
4545 636 624 569 159 73 40
BamHI G'GATC_C
Cuts at: 3976 4343 3976
Size: 367 6279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2620 3792 3967 5422 618
Size: 325 217 627 833 1172 175 1455 1842
Fragments arranged by size:
1842 1455 1172 833 627 325 217 175
BanII G_rGCy'C
Cuts at: 1391 2131 2348 2969 3315 3381 3851 3965 1391
Size: 740 217 621 346 66 470 114 4072
Fragments arranged by size:
4072 740 621 470 346 217 114 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3678 928
Size: 971 1779 3896
Fragments arranged by size:
3896 1779 971
BbvCI CC'TCA_GC
Cuts at: 3197 3788 3197
Size: 591 6055
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2762 2796 4837 4871 1903
Size: 34 825 34 2041 34 3678
Fragments arranged by size:
3678 2041 825 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4538 5748 4538
Size: 1210 5436
BclI T'GATC_A
Cuts at: 1548 2112 1548
Size: 564 6082
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2363 2552 5257
Size: 107 122 71 631 605 691 189 2705
Cuts at: 5257 136
Size: 1525
Fragments arranged by size:
2705 1525 691 631 605 189 122 107
71
BglII A'GATC_T
Cuts at: 2889 3402 6641 2889
Size: 513 3239 2894
Fragments arranged by size:
3239 2894 513
BlpI GC'TnA_GC
Cuts at: 2594 2846 3380 4063 2594
Size: 252 534 683 5177
Fragments arranged by size:
5177 683 534 252
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2348 2689 2969 3039 3250 4707 5863
Size: 341 217 341 280 70 211 1457 1156
Cuts at: 5863 1790
Size: 2573
Fragments arranged by size:
2573 1457 1156 341 341 280 217 211
70
BmgBI CAC'GTC
Cuts at: 1318 3193 1318
Size: 1875 4771
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4377 5335 442
Size: 416 53 83 399 24 2960 958 1753
Fragments arranged by size:
2960 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6646
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 6646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3236 5306 1239
Size: 1997 2070 2579
Fragments arranged by size:
2579 2070 1997
Bpu10I CC'TnA_GC
Cuts at: 2556 3197 3788 2556
Size: 641 591 5414
Fragments arranged by size:
5414 641 591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2813 4771 5636 5934 2813
Size: 1958 865 298 3525
Fragments arranged by size:
3525 1958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5309 882
Size: 617 3810 2219
Fragments arranged by size:
3810 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2362 4437 493
Size: 711 1158 2075 2702
Fragments arranged by size:
2702 2075 1158 711
BsaBI GATnn'nnATC
Cuts at: 6640 6640
Size: 6646
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3675 4453 4835 5470
Size: 53 83 186 936 2142 778 382 635
Cuts at: 5470 275
Size: 1451
Fragments arranged by size:
2142 1451 936 778 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5079 5477 5751 5898 6309 5079
Size: 398 274 147 411 5416
Fragments arranged by size:
5416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6421
Fragments arranged by size:
6421 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2398 3014 3920 1186
Size: 1212 616 906 3912
Fragments arranged by size:
3912 1212 906 616
BseYI C'CCAG_C
Cuts at: 2347 2749 2833 3498 3939 5849 6345 2347
Size: 402 84 665 441 1910 496 2648
Fragments arranged by size:
2648 1910 665 496 441 402 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 3815 1163
Size: 573 462 1617 3994
Fragments arranged by size:
3994 1617 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2070 3061 4096 4857 5885 1540
Size: 275 186 69 991 1035 761 1028 2301
Fragments arranged by size:
2301 1035 1028 991 761 275 186 69
BsiHKAI G_wGCw'C
Cuts at: 3039 3183 3315 3752 4707 4792 5863 3039
Size: 144 132 437 955 85 1071 3822
Fragments arranged by size:
3822 1071 955 437 144 132 85
BsmI GAATG_Cn'
Cuts at: 4157 4250 4157
Size: 93 6553
BsmBI CGTCTCn'nnnn_
Cuts at: 3803 3803
Size: 6646
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2348 2689 2969 3039 3183 3250
Size: 399 341 217 341 280 70 144 67
Cuts at: 3250 3315 3381 3752 3851 3965 4707 4792 5863
Size: 65 66 371 99 114 742 85 1071
Cuts at: 5863 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 371 341 341 280
217 144 114 99 85 70 67 66
65
BspEI T'CCGG_A
Cuts at: 6309 6309
Size: 6646
BspHI T'CATG_A
Cuts at: 4535 6265 4535
Size: 1730 4916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2091 3022 3273 3934 4001 844
Size: 1247 931 251 661 67 3489
Fragments arranged by size:
3489 1247 931 661 251 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2297 2614 2788 2899 3004 4533 1394
Size: 429 474 317 174 111 105 1529 3507
Fragments arranged by size:
3507 1529 474 429 317 174 111 105
BsrDI GCAATG_nn'
Cuts at: 65 1248 5141 5315 65
Size: 1183 3893 174 1396
Fragments arranged by size:
3893 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2640 3647 5290 5477 1700
Size: 110 830 1007 1643 187 2869
Fragments arranged by size:
2869 1643 1007 830 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6646
BssSI C'ACGA_G
Cuts at: 4706 5718 4706
Size: 1012 5634
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2805 3924 794
Size: 2011 1119 3516
Fragments arranged by size:
3516 2011 1119
BstBI TT'CG_AA
Cuts at: 5450 5450
Size: 6646
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6646
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2146 3089 3343 2035
Size: 111 943 254 5338
Fragments arranged by size:
5338 943 254 111
BstYI r'GATC_y
Cuts at: 1972 2889 3078 3402 3551 3762 3976 4343 4728
Size: 917 189 324 149 211 214 367 385
Cuts at: 4728 4745 6197 6283 6295 6641 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 917 385 367 346 324 214
211 189 149 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2731 3431 2731
Size: 700 5946
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2139 2469 2667 513
Size: 552 939 66 69 330 198 4492
Fragments arranged by size:
4492 939 552 330 198 69 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5907
Fragments arranged by size:
5907 683 56
BtsI GCAGTG_nn'
Cuts at: 4162 4956 4976 4162
Size: 794 20 5832
Fragments arranged by size:
5832 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2797 2832 529
Size: 35 2233 35 4343
Fragments arranged by size:
4343 2233 35 35
DraI TTT'AAA
Cuts at: 3950 3950
Size: 6646
DraIII CAC_nnn'GTG
Cuts at: 3511 3511
Size: 6646
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5653 809
Size: 507 4337 1802
Fragments arranged by size:
4337 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2121 2364
Size: 54 1425 50 275 18 237 54 243
Cuts at: 2364 2398 2638 2646 2664 3058 3069 3132 4093
Size: 34 240 8 18 394 11 63 961
Cuts at: 4093 4982 6611 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 961 889 394 275 243 240
237 63 54 54 50 43 34 18
18 11 8
EagI C'GGCC_G
Cuts at: 1537 1812 2067 3058 4093 1537
Size: 275 255 991 1035 4090
Fragments arranged by size:
4090 1035 991 275 255
EarI CTCTTCn'nnn_
Cuts at: 1353 2290 2702 4576 1353
Size: 937 412 1874 3423
Fragments arranged by size:
3423 1874 937 412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5233 5607 5753 387
Size: 1064 138 3644 374 146 1280
Fragments arranged by size:
3644 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3313 3313
Size: 6646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 2951 3236 3290 3893 4709 5306
Size: 393 639 680 285 54 603 816 597
Cuts at: 5306 6093 6361 1239
Size: 787 268 1524
Fragments arranged by size:
1524 816 787 680 639 603 597 393
285 268 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2771 3269 3365 3704 2771
Size: 498 96 339 5713
Fragments arranged by size:
5713 498 339 96
EcoO109I rG'GnC_Cy
Cuts at: 2127 2128 2344 2518 2725 2966 3262 2127
Size: 1 216 174 207 241 296 5511
Fragments arranged by size:
5511 296 241 216 207 174 1
EcoRI G'AATT_C
Cuts at: 3955 4333 3955
Size: 378 6268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3898 3898
Size: 6646
FspI TGC'GCA
Cuts at: 2798 5152 2798
Size: 2354 4292
FspAI rTGC'GCAy
Cuts at: 2798 2798
Size: 6646
HaeII r_GCGC'y
Cuts at: 1678 2315 5793 1678
Size: 637 3478 2531
Fragments arranged by size:
3478 2531 637
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 2447 2479 2932
Size: 32 135 32 441 32 402 32 453
Cuts at: 2932 2964 3332 3364 3781 3813 5287 5319 5361
Size: 32 368 32 417 32 1474 32 42
Cuts at: 5361 5393 1373
Size: 32 2626
Fragments arranged by size:
2626 1474 453 441 417 402 368 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3121 3990 4236 669
Size: 988 281 1183 869 246 3079
Fragments arranged by size:
3079 1183 988 869 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6646
HpaI GTT'AAC
Cuts at: 4236 4236
Size: 6646
KpnI G_GTAC'C
Cuts at: 3971 3971
Size: 6646
MfeI C'AATT_G
Cuts at: 4245 4245
Size: 6646
MluI A'CGCG_T
Cuts at: 6522 6522
Size: 6646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2967 3504 5760 5944 1305
Size: 1662 537 2256 184 2007
Fragments arranged by size:
2256 2007 1662 537 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2366 2648 3071 10
Size: 54 1425 343 534 282 423 3585
Fragments arranged by size:
3585 1425 534 423 343 282 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2030 2033 2144 3134 3632 3684 4604
Size: 648 864 3 111 990 498 52 920
Cuts at: 4604 4963 5122 518
Size: 359 159 2042
Fragments arranged by size:
2042 990 920 864 648 498 359 159
111 52 3
MspA1I CmG'CkG
Cuts at: 1606 4062 4739 5887 6132 1606
Size: 2456 677 1148 245 2120
Fragments arranged by size:
2456 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2642 2642
Size: 6646
NcoI C'CATG_G
Cuts at: 513 1065 2004 2469 513
Size: 552 939 465 4690
Fragments arranged by size:
4690 939 552 465
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6646
NgoMIV G'CCGG_C
Cuts at: 2640 2640
Size: 6646
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6646
NotI GC'GGCC_GC
Cuts at: 3058 4093 3058
Size: 1035 5611
NspI r_CATG'y
Cuts at: 1132 1229 2164 2261 2606 3157 3329 4004 5549
Size: 97 935 97 345 551 172 675 1545
Cuts at: 5549 1132
Size: 2229
Fragments arranged by size:
2229 1545 935 675 551 345 172 97
97
PasI CC'CwG_GG
Cuts at: 1348 2323 1348
Size: 975 5671
PciI A'CATG_T
Cuts at: 3325 5545 3325
Size: 2220 4426
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6646
PfoI T'CCnGG_A
Cuts at: 2853 2853
Size: 6646
PmeI GTTT'AAAC
Cuts at: 3950 3950
Size: 6646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 4715 4747 6228 6260 3623
Size: 32 1060 32 1481 32 4009
Fragments arranged by size:
4009 1481 1060 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 3262 2725
Size: 537 6109
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6646
PsiI TTA'TAA
Cuts at: 4216 4216
Size: 6646
PspOMI G'GGCC_C
Cuts at: 2127 2344 2965 2127
Size: 217 621 5808
Fragments arranged by size:
5808 621 217
PstI C_TGCA'G
Cuts at: 830 2088 2136 3320 3998 830
Size: 1258 48 1184 678 3478
Fragments arranged by size:
3478 1258 1184 678 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6646
SacI G_AGCT'C
Cuts at: 3315 3315
Size: 6646
SalI G'TCGA_C
Cuts at: 1936 3988 1936
Size: 2052 4594
SapI GCTCTTCn'nnn_
Cuts at: 2702 2702
Size: 6646
SbfI CC_TGCA'GG
Cuts at: 3998 3998
Size: 6646
ScaI AGT'ACT
Cuts at: 4894 6606 4894
Size: 1712 4934
SfcI C'TryA_G
Cuts at: 826 1045 2084 2132 3316 3913 3994 5129 5810
Size: 219 1039 48 1184 597 81 1135 681
Cuts at: 5810 6001 826
Size: 191 1471
Fragments arranged by size:
1471 1184 1135 1039 681 597 219 191
81 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6646
SmaI CCC'GGG
Cuts at: 1872 2609 3973 1872
Size: 737 1364 4545
Fragments arranged by size:
4545 1364 737
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2828 4750 5651 5913 820
Size: 197 926 885 1922 901 262 1553
Fragments arranged by size:
1922 1553 926 901 885 262 197
SnaBI TAC'GTA
Cuts at: 493 4437 493
Size: 3944 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6646
SphI G_CATG'C
Cuts at: 1132 2261 2606 4004 1132
Size: 1129 345 1398 3774
Fragments arranged by size:
3774 1398 1129 345
SspI AAT'ATT
Cuts at: 5 52 2204 4570 5
Size: 47 2152 2366 2081
Fragments arranged by size:
2366 2152 2081 47
StuI AGG'CCT
Cuts at: 1517 2046 2405 3168 3710 3911 1517
Size: 529 359 763 542 201 4252
Fragments arranged by size:
4252 763 542 529 359 201
StyI C'CwwG_G
Cuts at: 513 1065 2004 2469 4085 513
Size: 552 939 465 1616 3074
Fragments arranged by size:
3074 1616 939 552 465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4673 4690 4843 5028 5473 325
Size: 1255 3093 17 153 185 445 1498
Fragments arranged by size:
3093 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4892 6604 96
Size: 274 80 33 51 1443 2915 1712 138
Fragments arranged by size:
2915 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6606 6638 6606
Size: 32 6614
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6646
XbaI T'CTAG_A
Cuts at: 3982 3982
Size: 6646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2510 3697 2510
Size: 1187 5459
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6646
XmaI C'CCGG_G
Cuts at: 1870 2607 3971 1870
Size: 737 1364 4545
Fragments arranged by size:
4545 1364 737
XmnI GAAnn'nnTTC
Cuts at: 4775 4775
Size: 6646
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4454 276
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoRV FseI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI