Restriction map for
pFN21AE0989
(Circular) (Six-base) MAPSORT of: pFN21AE0989.seq Check: 3818 from: 1 to: 6574
pFN21AE0989 6574 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4384 278
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3917 1937
Size: 1980 4594
Acc65I G'GTAC_C
Cuts at: 3895 3895
Size: 6574
AclI AA'CG_TT
Cuts at: 1563 4701 5074 1563
Size: 3138 373 3063
Fragments arranged by size:
3138 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 2511 2529 3702 4637 6021 6289 1632
Size: 554 325 18 1173 935 1384 268 1917
Fragments arranged by size:
1917 1384 1173 935 554 325 268 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 5473 6450 1203
Size: 4270 977 1327
Fragments arranged by size:
4270 1327 977
AgeI A'CCGG_T
Cuts at: 5405 5405
Size: 6574
AhdI GACnn_n'nnGTC
Cuts at: 5303 5303
Size: 6574
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6574
AlwNI CAG_nnn'CTG
Cuts at: 3034 3061 4317 5889 3034
Size: 27 1256 1572 3719
Fragments arranged by size:
3719 1572 1256 27
ApaLI G'TGCA_C
Cuts at: 3810 3830 4631 5787 3810
Size: 20 801 1156 4597
Fragments arranged by size:
4597 1156 801 20
ApoI r'AATT_y
Cuts at: 1493 2637 3883 4109 4261 6370 1493
Size: 1144 1246 226 152 2109 1697
Fragments arranged by size:
2109 1697 1246 1144 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2270 5128 160
Size: 872 1238 2858 1606
Fragments arranged by size:
2858 1606 1238 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 3121 3899 1870
Size: 73 1178 778 4545
Fragments arranged by size:
4545 1178 778 73
BamHI G'GATC_C
Cuts at: 2806 3687 3904 4271 2806
Size: 881 217 367 5109
Fragments arranged by size:
5109 881 367 217
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3895 5350 618
Size: 325 217 627 2108 1455 1842
Fragments arranged by size:
2108 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3893 1391
Size: 2502 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5603
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4765 4799 1903
Size: 34 2828 34 3678
Fragments arranged by size:
3678 2828 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2678 4466 5676 2678
Size: 1788 1210 3576
Fragments arranged by size:
3576 1788 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6574
BfrBI ATG'CAT
Cuts at: 2139 2139
Size: 6574
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5185 136
Size: 107 122 71 631 605 3513 1525
Fragments arranged by size:
3513 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2522 6569 2522
Size: 4047 2527
BlpI GC'TnA_GC
Cuts at: 3991 3991
Size: 6574
Bme1580I G_kGCm'C
Cuts at: 1790 2506 3814 3834 4635 5791 1790
Size: 716 1308 20 801 1156 2573
Fragments arranged by size:
2573 1308 1156 801 716 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6574
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2716 3566 3856
Size: 416 53 83 399 24 1299 850 290
Cuts at: 3856 4305 5263 442
Size: 449 958 1753
Fragments arranged by size:
1753 1299 958 850 449 416 399 290
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3234 5234 1239
Size: 1995 2000 2579
Fragments arranged by size:
2579 2000 1995
Bpu10I CC'TnA_GC
Cuts at: 2967 3062 2967
Size: 95 6479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2167 4699 5564 5862 2167
Size: 2532 865 298 2879
Fragments arranged by size:
2879 2532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5237 882
Size: 617 3738 2219
Fragments arranged by size:
3738 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2046 4365 493
Size: 711 842 2319 2702
Fragments arranged by size:
2702 2319 842 711
BsaBI GATnn'nnATC
Cuts at: 6568 6568
Size: 6574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4381 4763 5398 275
Size: 53 83 186 936 2848 382 635 1451
Fragments arranged by size:
2848 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5007 5405 5679 5826 6237 5007
Size: 398 274 147 411 5344
Fragments arranged by size:
5344 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6349
Fragments arranged by size:
6349 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6574
BseYI C'CCAG_C
Cuts at: 3009 3222 5777 6273 3009
Size: 213 2555 496 3310
Fragments arranged by size:
3310 2555 496 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3689 1163
Size: 573 1953 4048
Fragments arranged by size:
4048 1953 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2822 4024 4785 5813 1540
Size: 275 186 821 1202 761 1028 2301
Fragments arranged by size:
2301 1202 1028 821 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3814 3834 4635 4720 5791 3814
Size: 20 801 85 1071 4597
Fragments arranged by size:
4597 1071 801 85 20
BsmI GAATG_Cn'
Cuts at: 2141 4085 4178 2141
Size: 1944 93 4537
Fragments arranged by size:
4537 1944 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2506 3814 3834 3893 4635 4720 5791
Size: 399 716 1308 20 59 742 85 1071
Cuts at: 5791 1391
Size: 2174
Fragments arranged by size:
2174 1308 1071 742 716 399 85 59
20
BspEI T'CCGG_A
Cuts at: 6237 6237
Size: 6574
BspHI T'CATG_A
Cuts at: 2784 4463 6193 2784
Size: 1679 1730 3165
Fragments arranged by size:
3165 1730 1679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3388 3929 844
Size: 2544 541 3489
Fragments arranged by size:
3489 2544 541
BsrBI CCG'CTC
Cuts at: 1394 1823 4461 1394
Size: 429 2638 3507
Fragments arranged by size:
3507 2638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3601 5069 5243 65
Size: 1183 2353 1468 174 1396
Fragments arranged by size:
2353 1468 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5218 5405 1700
Size: 110 3408 187 2869
Fragments arranged by size:
3408 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6574
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6574
BssSI C'ACGA_G
Cuts at: 4634 5646 4634
Size: 1012 5562
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3034 3578 794
Size: 2240 544 3790
Fragments arranged by size:
3790 2240 544
BstBI TT'CG_AA
Cuts at: 5378 5378
Size: 6574
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6574
BstXI CCAn_nnnn'nTGG
Cuts at: 2360 3556 2360
Size: 1196 5378
BstYI r'GATC_y
Cuts at: 1972 2522 2806 3687 3904 4271 4656 4673 6125
Size: 550 284 881 217 367 385 17 1452
Cuts at: 6125 6211 6223 6569 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 881 550 385 367 346 284
217 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3340 3340
Size: 6574
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6022
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5835
Fragments arranged by size:
5835 683 56
BtsI GCAGTG_nn'
Cuts at: 3032 4090 4884 4904 3032
Size: 1058 794 20 4702
Fragments arranged by size:
4702 1058 794 20
ClaI AT'CG_AT
Cuts at: 1554 2988 1554
Size: 1434 5140
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 2635 2913 3289 3878 2635
Size: 278 376 589 5331
Fragments arranged by size:
5331 589 376 278
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5581 809
Size: 507 4265 1802
Fragments arranged by size:
4265 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2675 3679
Size: 54 1425 50 275 18 264 581 1004
Cuts at: 3679 3852 4021 4910 6539 8
Size: 173 169 889 1629 43
Fragments arranged by size:
1629 1425 1004 889 581 275 264 173
169 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4021 1537
Size: 275 2209 4090
Fragments arranged by size:
4090 2209 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 3222 4504 1353
Size: 752 1117 1282 3423
Fragments arranged by size:
3423 1282 1117 752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5161 5535 5681 387
Size: 1064 138 3572 374 146 1280
Fragments arranged by size:
3572 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2511 2529 3234 3702 4637 5234
Size: 393 554 325 18 705 468 935 597
Cuts at: 5234 6021 6289 1239
Size: 787 268 1524
Fragments arranged by size:
1524 935 787 705 597 554 468 393
325 268 18
EcoO109I rG'GnC_Cy
Cuts at: 2876 3560 2876
Size: 684 5890
EcoRI G'AATT_C
Cuts at: 3883 4261 3883
Size: 378 6196
EcoRV GAT'ATC
Cuts at: 3461 3461
Size: 6574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3508 3508
Size: 6574
FspI TGC'GCA
Cuts at: 5080 5080
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 5721 1678
Size: 4043 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 5215 5247 5289
Size: 32 135 32 896 32 2715 32 42
Cuts at: 5289 5321 1373
Size: 32 2626
Fragments arranged by size:
2715 2626 896 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3417 3918 4164 669
Size: 988 281 1479 501 246 3079
Fragments arranged by size:
3079 1479 988 501 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6574
HpaI GTT'AAC
Cuts at: 4164 4164
Size: 6574
KpnI G_GTAC'C
Cuts at: 3899 3899
Size: 6574
MfeI C'AATT_G
Cuts at: 4173 4173
Size: 6574
MluI A'CGCG_T
Cuts at: 6450 6450
Size: 6574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2542 2980 3213 3372 3449 5688 5872 1305
Size: 1237 438 233 159 77 2239 184 2007
Fragments arranged by size:
2239 2007 1237 438 233 184 159 77
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 2677 3681 3854 10
Size: 54 1425 343 264 581 1004 173 2730
Fragments arranged by size:
2730 1425 1004 581 343 264 173 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 2184 2358 2549 3174 4532 4891
Size: 648 855 163 174 191 625 1358 359
Cuts at: 4891 5050 518
Size: 159 2042
Fragments arranged by size:
2042 1358 855 648 625 359 191 174
163 159
MspA1I CmG'CkG
Cuts at: 1606 2692 2923 3677 3990 4667 5815 6060 1606
Size: 1086 231 754 313 677 1148 245 2120
Fragments arranged by size:
2120 1148 1086 754 677 313 245 231
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6022
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6574
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NsiI A_TGCA'T
Cuts at: 2141 2141
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 3142 3932 5477 1132
Size: 97 1913 790 1545 2229
Fragments arranged by size:
2229 1913 1545 790 97
PasI CC'CwG_GG
Cuts at: 1348 2606 1348
Size: 1258 5316
PciI A'CATG_T
Cuts at: 5473 5473
Size: 6574
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6574
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4643 4675 6156 6188 4643
Size: 32 1481 32 5029
Fragments arranged by size:
5029 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3560 3560
Size: 6574
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 3774 4144 3774
Size: 370 6204
PstI C_TGCA'G
Cuts at: 830 3039 3926 830
Size: 2209 887 3478
Fragments arranged by size:
3478 2209 887
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 2692 2923 3677 2692
Size: 231 754 5589
Fragments arranged by size:
5589 754 231
SalI G'TCGA_C
Cuts at: 1936 3916 1936
Size: 1980 4594
SanDI GG'GwC_CC
Cuts at: 3560 3560
Size: 6574
SbfI CC_TGCA'GG
Cuts at: 3926 3926
Size: 6574
ScaI AGT'ACT
Cuts at: 3440 4822 6534 3440
Size: 1382 1712 3480
Fragments arranged by size:
3480 1712 1382
SfcI C'TryA_G
Cuts at: 826 1045 3035 3780 3922 5057 5738 5929 826
Size: 219 1990 745 142 1135 681 191 1471
Fragments arranged by size:
1990 1471 1135 745 681 219 191 142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6574
SmaI CCC'GGG
Cuts at: 1872 3901 1872
Size: 2029 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2146 4678 5579 5841 820
Size: 197 926 203 2532 901 262 1553
Fragments arranged by size:
2532 1553 926 901 262 203 197
SnaBI TAC'GTA
Cuts at: 493 4365 493
Size: 3872 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 3932 1132
Size: 2800 3774
SspI AAT'ATT
Cuts at: 5 52 2317 2331 3799 4498 5
Size: 47 2265 14 1468 699 2081
Fragments arranged by size:
2265 2081 1468 699 47 14
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6574
StyI C'CwwG_G
Cuts at: 513 1065 4013 513
Size: 552 2948 3074
Fragments arranged by size:
3074 2948 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2804 2836 2862 3269 3838 4601 4618
Size: 1255 1224 32 26 407 569 763 17
Cuts at: 4618 4771 4956 5401 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1224 763 569 445 407 185
153 32 26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3438 4820 6532
Size: 274 80 33 51 1443 1461 1382 1712
Cuts at: 6532 96
Size: 138
Fragments arranged by size:
1712 1461 1443 1382 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6534 6566 6534
Size: 32 6542
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6574
XbaI T'CTAG_A
Cuts at: 3910 3910
Size: 6574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 6574
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6574
XmaI C'CCGG_G
Cuts at: 1870 3899 1870
Size: 2029 4545
XmnI GAAnn'nnTTC
Cuts at: 2775 4703 2775
Size: 1928 4646
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4382 276
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI