Restriction map for
pFN21AE0985
(Circular) (Six-base) MAPSORT of: pFN21AE0985.seq Check: 7608 from: 1 to: 6493
pFN21AE0985 6493 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2443 2443
Size: 6493
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4303 278
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3836 1937
Size: 1899 4594
Acc65I G'GTAC_C
Cuts at: 2195 3814 2195
Size: 1619 4874
AclI AA'CG_TT
Cuts at: 1563 3483 4620 4993 1563
Size: 1920 1137 373 3063
Fragments arranged by size:
3063 1920 1137 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 3341 4556 5940 6208 1632
Size: 542 1167 1215 1384 268 1917
Fragments arranged by size:
1917 1384 1215 1167 542 268
AfeI AGC'GCT
Cuts at: 3736 3736
Size: 6493
AflII C'TTAA_G
Cuts at: 820 1017 3665 3683 820
Size: 197 2648 18 3630
Fragments arranged by size:
3630 2648 197 18
AflIII A'CryG_T
Cuts at: 1203 3396 5392 6369 1203
Size: 2193 1996 977 1327
Fragments arranged by size:
2193 1996 1327 977
AgeI A'CCGG_T
Cuts at: 5324 5324
Size: 6493
AhdI GACnn_n'nnGTC
Cuts at: 5222 5222
Size: 6493
AleI CACnn'nnGTG
Cuts at: 1166 3155 1166
Size: 1989 4504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6493
AlwNI CAG_nnn'CTG
Cuts at: 4236 5808 4236
Size: 1572 4921
ApaI G_GGCC'C
Cuts at: 2262 2283 3586 2262
Size: 21 1303 5169
Fragments arranged by size:
5169 1303 21
ApaLI G'TGCA_C
Cuts at: 4550 5706 4550
Size: 1156 5337
ApoI r'AATT_y
Cuts at: 1493 2037 2849 3802 4028 4180 6289 1493
Size: 544 812 953 226 152 2109 1697
Fragments arranged by size:
2109 1697 953 812 544 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5047 160
Size: 872 4015 1606
Fragments arranged by size:
4015 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
AvaI C'yCGr_G
Cuts at: 1870 1943 2677 3766 3818 1870
Size: 73 734 1089 52 4545
Fragments arranged by size:
4545 1089 734 73 52
BamHI G'GATC_C
Cuts at: 2140 2368 2563 3183 3823 4190 2140
Size: 228 195 620 640 367 4443
Fragments arranged by size:
4443 640 620 367 228 195
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2062 2195 2539 3814 5269
Size: 325 217 627 275 133 344 1275 1455
Cuts at: 5269 618
Size: 1842
Fragments arranged by size:
1842 1455 1275 627 344 325 275 217
133
BanII G_rGCy'C
Cuts at: 1391 2262 2283 2432 2526 3105 3586 3812 1391
Size: 871 21 149 94 579 481 226 4072
Fragments arranged by size:
4072 871 579 481 226 149 94 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5522
BbvCI CC'TCA_GC
Cuts at: 2825 2825
Size: 6493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4684 4718 1903
Size: 34 2747 34 3678
Fragments arranged by size:
3678 2747 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4385 5595 4385
Size: 1210 5283
BclI T'GATC_A
Cuts at: 1548 3414 1548
Size: 1866 4627
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2649 5104 136
Size: 107 122 71 631 605 977 2455 1525
Fragments arranged by size:
2455 1525 977 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3171 3675 6488 3171
Size: 504 2813 3176
Fragments arranged by size:
3176 2813 504
BlpI GC'TnA_GC
Cuts at: 2343 3431 3910 2343
Size: 1088 479 4926
Fragments arranged by size:
4926 1088 479
Bme1580I G_kGCm'C
Cuts at: 1790 2262 2283 2544 3586 3775 4554 5710 1790
Size: 472 21 261 1042 189 779 1156 2573
Fragments arranged by size:
2573 1156 1042 779 472 261 189 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6493
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3595 4224 5182
Size: 416 53 83 399 24 2178 629 958
Cuts at: 5182 442
Size: 1753
Fragments arranged by size:
2178 1753 958 629 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2458 3011 5153 1239
Size: 1219 553 2142 2579
Fragments arranged by size:
2579 2142 1219 553
Bpu10I CC'TnA_GC
Cuts at: 2825 2825
Size: 6493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3209 4618 5483 5781 2915
Size: 294 1409 865 298 3627
Fragments arranged by size:
3627 1409 865 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2312 3464 3509 5156 882
Size: 617 813 1152 45 1647 2219
Fragments arranged by size:
2219 1647 1152 813 617 45
BsaAI yAC'GTr
Cuts at: 493 1204 4284 493
Size: 711 3080 2702
Fragments arranged by size:
3080 2702 711
BsaBI GATnn'nnATC
Cuts at: 6487 6487
Size: 6493
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3661 4300 4682 5317
Size: 53 83 186 936 2128 639 382 635
Cuts at: 5317 275
Size: 1451
Fragments arranged by size:
2128 1451 936 639 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2137 4926 5324 5598 5745 6156 2137
Size: 2789 398 274 147 411 2474
Fragments arranged by size:
2789 2474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3365 3395 3539 3569 1178
Size: 30 165 30 1962 30 144 30 4102
Fragments arranged by size:
4102 1962 165 144 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2768 1186
Size: 1582 4911
BseYI C'CCAG_C
Cuts at: 2032 2276 2298 2453 5696 6192 2032
Size: 244 22 155 3243 496 2333
Fragments arranged by size:
3243 2333 496 244 155 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5920
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3943 4704 5732 1540
Size: 275 186 1942 761 1028 2301
Fragments arranged by size:
2301 1942 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2526 3105 4554 4639 5710 2526
Size: 579 1449 85 1071 3309
Fragments arranged by size:
3309 1449 1071 579 85
BsmI GAATG_Cn'
Cuts at: 4004 4097 4004
Size: 93 6400
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2283 2432 2526 2544 3105 3586
Size: 399 472 21 149 94 18 561 481
Cuts at: 3586 3775 3812 4554 4639 5710 1391
Size: 189 37 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 561 481 472 399 189
149 94 85 37 21 18
BspEI T'CCGG_A
Cuts at: 2137 6156 2137
Size: 4019 2474
BspHI T'CATG_A
Cuts at: 3003 3696 4382 6112 3003
Size: 693 686 1730 3384
Fragments arranged by size:
3384 1730 693 686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2443 2915 3848 844
Size: 1599 472 933 3489
Fragments arranged by size:
3489 1599 933 472
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 4380 1394
Size: 429 1612 945 3507
Fragments arranged by size:
3507 1612 945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3723 4988 5162 65
Size: 1183 2475 1265 174 1396
Fragments arranged by size:
2475 1396 1265 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5137 5324 1700
Size: 110 3327 187 2869
Fragments arranged by size:
3327 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6493
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6493
BssSI C'ACGA_G
Cuts at: 4553 5565 4553
Size: 1012 5481
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6493
BstBI TT'CG_AA
Cuts at: 5297 5297
Size: 6493
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6493
BstXI CCAn_nnnn'nTGG
Cuts at: 2372 2372
Size: 6493
BstYI r'GATC_y
Cuts at: 1972 2140 2368 2563 3171 3183 3675 3823 4190
Size: 168 228 195 608 12 492 148 367
Cuts at: 4190 4575 4592 6044 6130 6142 6488 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 608 492 385 367 346 228
195 168 148 86 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 2356 3357 2356
Size: 1001 5492
BtgI C'CryG_G
Cuts at: 513 1065 2004 2262 2559 3153 513
Size: 552 939 258 297 594 3853
Fragments arranged by size:
3853 939 594 552 297 258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5754
Fragments arranged by size:
5754 683 56
BtsI GCAGTG_nn'
Cuts at: 2278 2851 4009 4803 4823 2278
Size: 573 1158 794 20 3948
Fragments arranged by size:
3948 1158 794 573 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6458
DraI TTT'AAA
Cuts at: 3797 3797
Size: 6493
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5500 809
Size: 507 4184 1802
Fragments arranged by size:
4184 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2493 2650 3940
Size: 54 1425 50 275 18 663 157 1290
Cuts at: 3940 4829 6458 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1290 889 663 275 157 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3940 1537
Size: 275 2128 4090
Fragments arranged by size:
4090 2128 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3110 3194 3507 3695 4423 1353
Size: 1757 84 313 188 728 3423
Fragments arranged by size:
3423 1757 728 313 188 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3111 3650 5080 5454 5600 387
Size: 1064 138 1522 539 1430 374 146 1280
Fragments arranged by size:
1522 1430 1280 1064 539 374 146 138
EcoICRI GAG'CTC
Cuts at: 2524 3103 2524
Size: 579 5914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2458 3011 3341 4556 5153 5940
Size: 393 542 284 553 330 1215 597 787
Cuts at: 5940 6208 1239
Size: 268 1524
Fragments arranged by size:
1524 1215 787 597 553 542 393 330
284 268
EcoO109I rG'GnC_Cy
Cuts at: 2072 2228 2258 2280 2731 3336 3582 2072
Size: 156 30 22 451 605 246 4983
Fragments arranged by size:
4983 605 451 246 156 30 22
EcoRI G'AATT_C
Cuts at: 2037 2849 3802 4180 2037
Size: 812 953 378 4350
Fragments arranged by size:
4350 953 812 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3346 3736 3346
Size: 390 6103
FspI TGC'GCA
Cuts at: 4999 4999
Size: 6493
HaeII r_GCGC'y
Cuts at: 1678 2876 3709 3738 5640 1678
Size: 1198 833 29 1902 2531
Fragments arranged by size:
2531 1902 1198 833 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3335 3365 3367 3397 3515
Size: 32 135 32 1763 30 2 30 118
Cuts at: 3515 3539 3547 3571 5134 5166 5208 5240 1373
Size: 24 8 24 1563 32 42 32 2626
Fragments arranged by size:
2626 1763 1563 135 118 42 32 32
32 32 30 30 24 24 8 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3250 3837 4083 669
Size: 988 281 1312 587 246 3079
Fragments arranged by size:
3079 1312 988 587 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6493
HpaI GTT'AAC
Cuts at: 4083 4083
Size: 6493
KpnI G_GTAC'C
Cuts at: 2199 3818 2199
Size: 1619 4874
MfeI C'AATT_G
Cuts at: 4092 4092
Size: 6493
MluI A'CGCG_T
Cuts at: 6369 6369
Size: 6493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3582 5607 5791 1305
Size: 2277 2025 184 2007
Fragments arranged by size:
2277 2025 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2495 2652 10
Size: 54 1425 343 663 157 3851
Fragments arranged by size:
3851 1425 663 343 157 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3155 3422 4451 4810 4969 518
Size: 648 1989 267 1029 359 159 2042
Fragments arranged by size:
2042 1989 1029 648 359 267 159
MspA1I CmG'CkG
Cuts at: 1606 2264 2457 3013 3037 3909 4586 5734 5979
Size: 658 193 556 24 872 677 1148 245
Cuts at: 5979 1606
Size: 2120
Fragments arranged by size:
2120 1148 872 677 658 556 245 193
24
NcoI C'CATG_G
Cuts at: 513 1065 2004 3153 513
Size: 552 939 1149 3853
Fragments arranged by size:
3853 1149 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6493
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6493
NotI GC'GGCC_GC
Cuts at: 3940 3940
Size: 6493
NspI r_CATG'y
Cuts at: 1132 1229 3400 3427 3851 5396 1132
Size: 97 2171 27 424 1545 2229
Fragments arranged by size:
2229 2171 1545 424 97 27
PasI CC'CwG_GG
Cuts at: 1348 2382 1348
Size: 1034 5459
PciI A'CATG_T
Cuts at: 3396 5392 3396
Size: 1996 4497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2773 1108
Size: 1665 4828
PfoI T'CCnGG_A
Cuts at: 2371 2371
Size: 6493
PmeI GTTT'AAAC
Cuts at: 3797 3797
Size: 6493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4562 4594 6075 6107 4562
Size: 32 1481 32 4948
Fragments arranged by size:
4948 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2072 3336 2072
Size: 1264 5229
PshAI GACnn'nnGTC
Cuts at: 1613 2324 1613
Size: 711 5782
PsiI TTA'TAA
Cuts at: 4063 4063
Size: 6493
PspOMI G'GGCC_C
Cuts at: 2258 2279 3582 2258
Size: 21 1303 5169
Fragments arranged by size:
5169 1303 21
PspXI vC'TCGA_Gb
Cuts at: 2677 2677
Size: 6493
PstI C_TGCA'G
Cuts at: 830 2101 3845 830
Size: 1271 1744 3478
Fragments arranged by size:
3478 1744 1271
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6493
PvuII CAG'CTG
Cuts at: 2457 3013 3037 2457
Size: 556 24 5913
Fragments arranged by size:
5913 556 24
SacI G_AGCT'C
Cuts at: 2526 3105 2526
Size: 579 5914
SacII CC_GC'GG
Cuts at: 2265 2265
Size: 6493
SalI G'TCGA_C
Cuts at: 1936 3835 1936
Size: 1899 4594
SanDI GG'GwC_CC
Cuts at: 3336 3336
Size: 6493
SapI GCTCTTCn'nnn_
Cuts at: 3110 3110
Size: 6493
SbfI CC_TGCA'GG
Cuts at: 3845 3845
Size: 6493
ScaI AGT'ACT
Cuts at: 4741 6453 4741
Size: 1712 4781
SfcI C'TryA_G
Cuts at: 826 1045 2097 3841 4976 5657 5848 826
Size: 219 1052 1744 1135 681 191 1471
Fragments arranged by size:
1744 1471 1135 1052 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2649 2649
Size: 6493
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6493
SmaI CCC'GGG
Cuts at: 1872 3820 1872
Size: 1948 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2677 2930 3224 3665 3683 4597
Size: 197 926 734 253 294 441 18 914
Cuts at: 4597 5498 5760 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 914 901 734 441 294 262
253 197 18
SnaBI TAC'GTA
Cuts at: 493 4284 493
Size: 3791 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6493
SphI G_CATG'C
Cuts at: 1132 3851 1132
Size: 2719 3774
SspI AAT'ATT
Cuts at: 5 52 4417 5
Size: 47 4365 2081
Fragments arranged by size:
4365 2081 47
StuI AGG'CCT
Cuts at: 1517 2928 1517
Size: 1411 5082
StyI C'CwwG_G
Cuts at: 513 1065 2004 2224 2337 2577 2865 2943 3153
Size: 552 939 220 113 240 288 78 210
Cuts at: 3153 3932 513
Size: 779 3074
Fragments arranged by size:
3074 939 779 552 288 240 220 210
113 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2161 3155 4520 4537 4690 4875 5320
Size: 1255 581 994 1365 17 153 185 445
Cuts at: 5320 325
Size: 1498
Fragments arranged by size:
1498 1365 1255 994 581 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3084 4739 6451
Size: 274 80 33 51 1443 1107 1655 1712
Cuts at: 6451 96
Size: 138
Fragments arranged by size:
1712 1655 1443 1107 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6453 6485 6453
Size: 32 6461
Tth111I GACn'n_nGTC
Cuts at: 1316 3551 1316
Size: 2235 4258
XbaI T'CTAG_A
Cuts at: 3829 3829
Size: 6493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3262 3262
Size: 6493
XhoI C'TCGA_G
Cuts at: 1943 2677 1943
Size: 734 5759
XmaI C'CCGG_G
Cuts at: 1870 3818 1870
Size: 1948 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 4622 3719
Size: 903 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4301 276
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI
RsrII SexAI SfoI SrfI SwaI