Restriction map for
pFN21AE0983
(Circular) (Six-base) MAPSORT of: pFN21AE0983.seq Check: 5970 from: 1 to: 6469
pFN21AE0983 6469 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2659 2659
Size: 6469
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3733 3812 1937
Size: 1796 79 4594
Fragments arranged by size:
4594 1796 79
Acc65I G'GTAC_C
Cuts at: 3790 3790
Size: 6469
AclI AA'CG_TT
Cuts at: 1563 2814 4596 4969 1563
Size: 1251 1782 373 3063
Fragments arranged by size:
3063 1782 1251 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 3032 4532 5916 6184 1632
Size: 833 567 1500 1384 268 1917
Fragments arranged by size:
1917 1500 1384 833 567 268
AfeI AGC'GCT
Cuts at: 2738 2738
Size: 6469
AflII C'TTAA_G
Cuts at: 820 1017 2783 820
Size: 197 1766 4506
Fragments arranged by size:
4506 1766 197
AflIII A'CryG_T
Cuts at: 1203 5368 6345 1203
Size: 4165 977 1327
Fragments arranged by size:
4165 1327 977
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 3073 3610 5198 3073
Size: 537 1588 4344
Fragments arranged by size:
4344 1588 537
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3767 1184
Size: 2583 3886
AlwNI CAG_nnn'CTG
Cuts at: 4212 5784 4212
Size: 1572 4897
ApaLI G'TGCA_C
Cuts at: 4526 5682 4526
Size: 1156 5313
ApoI r'AATT_y
Cuts at: 1493 2276 2505 3778 4004 4156 6265 1493
Size: 783 229 1273 226 152 2109 1697
Fragments arranged by size:
2109 1697 1273 783 229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2493 3333 5023 160
Size: 872 1461 840 1690 1606
Fragments arranged by size:
1690 1606 1461 872 840
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 2607 3794 1870
Size: 73 664 1187 4545
Fragments arranged by size:
4545 1187 664 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2930 2963 2930
Size: 33 6436
BamHI G'GATC_C
Cuts at: 3799 4166 3799
Size: 367 6102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2407 2419 3790 5245 618
Size: 325 217 627 620 12 1371 1455 1842
Fragments arranged by size:
1842 1455 1371 627 620 325 217 12
BanII G_rGCy'C
Cuts at: 1391 2064 3136 3788 1391
Size: 673 1072 652 4072
Fragments arranged by size:
4072 1072 673 652
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2191 3503 928
Size: 971 292 1312 3894
Fragments arranged by size:
3894 1312 971 292
BbvCI CC'TCA_GC
Cuts at: 2757 2757
Size: 6469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4660 4694 1903
Size: 34 2723 34 3678
Fragments arranged by size:
3678 2723 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4361 5571 4361
Size: 1210 5259
BclI T'GATC_A
Cuts at: 1548 2686 1548
Size: 1138 5331
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5080 136
Size: 107 122 71 631 605 3408 1525
Fragments arranged by size:
3408 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6464 6464
Size: 6469
BlpI GC'TnA_GC
Cuts at: 2876 3886 2876
Size: 1010 5459
Bme1580I G_kGCm'C
Cuts at: 1790 4530 5686 1790
Size: 2740 1156 2573
Fragments arranged by size:
2740 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6469
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2947 3460 3526
Size: 416 53 83 399 24 1530 513 66
Cuts at: 3526 4200 5158 442
Size: 674 958 1753
Fragments arranged by size:
1753 1530 958 674 513 416 399 83
66 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2415 3120 3255 3323 3657 5129 1239
Size: 1176 705 135 68 334 1472 2579
Fragments arranged by size:
2579 1472 1176 705 334 135 68
Bpu10I CC'TnA_GC
Cuts at: 2757 2757
Size: 6469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3531 4594 5459 5757 3531
Size: 1063 865 298 4243
Fragments arranged by size:
4243 1063 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2604 3243 5132 882
Size: 617 1105 639 1889 2219
Fragments arranged by size:
2219 1889 1105 639 617
BsaAI yAC'GTr
Cuts at: 493 1204 2707 3413 4260 493
Size: 711 1503 706 847 2702
Fragments arranged by size:
2702 1503 847 711 706
BsaBI GATnn'nnATC
Cuts at: 6463 6463
Size: 6469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4276 4658 5293 275
Size: 53 83 186 936 2743 382 635 1451
Fragments arranged by size:
2743 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2766 2844 4902 5300 5574 5721 6132 2766
Size: 78 2058 398 274 147 411 3103
Fragments arranged by size:
3103 2058 411 398 274 147 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6244
Fragments arranged by size:
6244 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2774 1186
Size: 1588 4881
BseYI C'CCAG_C
Cuts at: 2070 3541 5672 6168 2070
Size: 1471 2131 496 2371
Fragments arranged by size:
2371 2131 1471 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5896
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3919 4680 5708 1540
Size: 275 186 1918 761 1028 2301
Fragments arranged by size:
2301 1918 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3136 4530 4615 5686 3136
Size: 1394 85 1071 3919
Fragments arranged by size:
3919 1394 1071 85
BsmI GAATG_Cn'
Cuts at: 3980 4073 3980
Size: 93 6376
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 3136 3788 4530 4615 5686 1391
Size: 399 274 1072 652 742 85 1071 2174
Fragments arranged by size:
2174 1072 1071 742 652 399 274 85
BspEI T'CCGG_A
Cuts at: 2766 2844 6132 2766
Size: 78 3288 3103
Fragments arranged by size:
3288 3103 78
BspHI T'CATG_A
Cuts at: 2109 4358 6088 2109
Size: 2249 1730 2490
Fragments arranged by size:
2490 2249 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2659 3824 844
Size: 1815 1165 3489
Fragments arranged by size:
3489 1815 1165
BsrBI CCG'CTC
Cuts at: 1394 1823 4356 1394
Size: 429 2533 3507
Fragments arranged by size:
3507 2533 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2734 4964 5138 65
Size: 1183 1486 2230 174 1396
Fragments arranged by size:
2230 1486 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5113 5300 1700
Size: 110 3303 187 2869
Fragments arranged by size:
3303 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6469
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 2019 4529 5541 2019
Size: 2510 1012 2947
Fragments arranged by size:
2947 2510 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6469
BstBI TT'CG_AA
Cuts at: 5273 5273
Size: 6469
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6469
BstXI CCAn_nnnn'nTGG
Cuts at: 3380 3380
Size: 6469
BstYI r'GATC_y
Cuts at: 1972 2763 3799 4166 4551 4568 6020 6106 6118
Size: 791 1036 367 385 17 1452 86 12
Cuts at: 6118 6464 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1036 791 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3734 3734
Size: 6469
BtgI C'CryG_G
Cuts at: 513 1065 2004 3040 513
Size: 552 939 1036 3942
Fragments arranged by size:
3942 1036 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5730
Fragments arranged by size:
5730 683 56
BtsI GCAGTG_nn'
Cuts at: 2466 3001 3751 3985 4779 4799 2466
Size: 535 750 234 794 20 4136
Fragments arranged by size:
4136 794 750 535 234 20
ClaI AT'CG_AT
Cuts at: 1554 2626 1554
Size: 1072 5397
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6434
DraI TTT'AAA
Cuts at: 3773 3773
Size: 6469
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5476 809
Size: 507 4160 1802
Fragments arranged by size:
4160 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2673 3916 4805
Size: 54 1425 50 275 18 843 1243 889
Cuts at: 4805 6434 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1243 889 843 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3233 4399 1353
Size: 1880 1166 3423
Fragments arranged by size:
3423 1880 1166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2047 5056 5430 5576 387
Size: 1064 138 458 3009 374 146 1280
Fragments arranged by size:
3009 1280 1064 458 374 146 138
EcoICRI GAG'CTC
Cuts at: 3134 3134
Size: 6469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2415 2465 3032 3120 3255 3323 3657
Size: 393 783 50 567 88 135 68 334
Cuts at: 3657 4532 5129 5916 6184 1239
Size: 875 597 787 268 1524
Fragments arranged by size:
1524 875 787 783 597 567 393 334
268 135 88 68 50
EcoO109I rG'GnC_Cy
Cuts at: 2589 3431 3520 2589
Size: 842 89 5538
Fragments arranged by size:
5538 842 89
EcoRI G'AATT_C
Cuts at: 3778 4156 3778
Size: 378 6091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3579 3579
Size: 6469
FspI TGC'GCA
Cuts at: 2726 4975 2726
Size: 2249 4220
HaeII r_GCGC'y
Cuts at: 1678 2740 5616 1678
Size: 1062 2876 2531
Fragments arranged by size:
2876 2531 1062
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3059 3091 3210 3242 3529
Size: 32 135 32 1487 32 119 32 287
Cuts at: 3529 3561 3596 3628 5110 5142 5184 5216 1373
Size: 32 35 32 1482 32 42 32 2626
Fragments arranged by size:
2626 1487 1482 287 135 119 42 35
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3813 4059 669
Size: 988 281 1875 246 3079
Fragments arranged by size:
3079 1875 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3695 748
Size: 2947 3522
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KpnI G_GTAC'C
Cuts at: 3794 3794
Size: 6469
MfeI C'AATT_G
Cuts at: 3171 4068 3171
Size: 897 5572
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2415 3661 5583 5767 1305
Size: 1110 1246 1922 184 2007
Fragments arranged by size:
2007 1922 1246 1110 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 10
Size: 54 1425 343 843 3804
Fragments arranged by size:
3804 1425 843 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 4427 4786 4945 518
Size: 648 942 2319 359 159 2042
Fragments arranged by size:
2319 2042 942 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2266 2756 3081 3885 4562 5710 5955
Size: 471 189 490 325 804 677 1148 245
Cuts at: 5955 1606
Size: 2120
Fragments arranged by size:
2120 1148 804 677 490 471 325 245
189
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4978
Fragments arranged by size:
4978 939 552
NdeI CA'TA_TG
Cuts at: 387 3179 387
Size: 2792 3677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 2827 3827 5372 1132
Size: 97 1598 1000 1545 2229
Fragments arranged by size:
2229 1598 1545 1000 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6469
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2827 3655 3680 1108
Size: 1719 828 25 3897
Fragments arranged by size:
3897 1719 828 25
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 6051 6083 4538
Size: 32 1481 32 4924
Fragments arranged by size:
4924 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3431 3520 3431
Size: 89 6380
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6469
PsiI TTA'TAA
Cuts at: 4039 4039
Size: 6469
PstI C_TGCA'G
Cuts at: 830 3821 830
Size: 2991 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
PvuII CAG'CTG
Cuts at: 2266 2756 3081 2266
Size: 490 325 5654
Fragments arranged by size:
5654 490 325
SacI G_AGCT'C
Cuts at: 3136 3136
Size: 6469
SalI G'TCGA_C
Cuts at: 1936 3811 1936
Size: 1875 4594
SanDI GG'GwC_CC
Cuts at: 3431 3520 3431
Size: 89 6380
SbfI CC_TGCA'GG
Cuts at: 3821 3821
Size: 6469
ScaI AGT'ACT
Cuts at: 3326 4717 6429 3326
Size: 1391 1712 3366
Fragments arranged by size:
3366 1712 1391
SfcI C'TryA_G
Cuts at: 826 1045 3127 3160 3817 4952 5633 5824 826
Size: 219 2082 33 657 1135 681 191 1471
Fragments arranged by size:
2082 1471 1135 681 657 219 191 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 3796 1872
Size: 1924 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2607 2783 3546 4573 5474 5736
Size: 197 926 664 176 763 1027 901 262
Cuts at: 5736 820
Size: 1553
Fragments arranged by size:
1553 1027 926 901 763 664 262 197
176
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3827 1132
Size: 2695 3774
SspI AAT'ATT
Cuts at: 5 52 4393 5
Size: 47 4341 2081
Fragments arranged by size:
4341 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6469
StyI C'CwwG_G
Cuts at: 513 1065 2004 2403 3057 3339 3563 3908 513
Size: 552 939 399 654 282 224 345 3074
Fragments arranged by size:
3074 939 654 552 399 345 282 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2194 2425 4496 4513 4666 4851 5296
Size: 1255 614 231 2071 17 153 185 445
Cuts at: 5296 325
Size: 1498
Fragments arranged by size:
2071 1498 1255 614 445 231 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2149 3324 4715
Size: 274 80 33 51 1443 172 1175 1391
Cuts at: 4715 6427 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1391 1175 274 172 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6429 6461 6429
Size: 32 6437
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6469
XbaI T'CTAG_A
Cuts at: 3805 3805
Size: 6469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3177 3177
Size: 6469
XhoI C'TCGA_G
Cuts at: 1943 2607 1943
Size: 664 5805
XmaI C'CCGG_G
Cuts at: 1870 3794 1870
Size: 1924 4545
XmnI GAAnn'nnTTC
Cuts at: 3024 4598 3024
Size: 1574 4895
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI