Restriction map for
pFN21AE0981
(Circular) (Six-base) MAPSORT of: pFN21AE0981.seq Check: 9615 from: 1 to: 6445
pFN21AE0981 6445 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2484 2484
Size: 6445
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4255 278
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2492 3573 3788 1937
Size: 555 1081 215 4594
Fragments arranged by size:
4594 1081 555 215
Acc65I G'GTAC_C
Cuts at: 3766 3766
Size: 6445
AclI AA'CG_TT
Cuts at: 1563 3099 4572 4945 1563
Size: 1536 1473 373 3063
Fragments arranged by size:
3063 1536 1473 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2074 2297 2770 2828 4508 5892 6160 1632
Size: 442 223 473 58 1680 1384 268 1917
Fragments arranged by size:
1917 1680 1384 473 442 268 223 58
AfeI AGC'GCT
Cuts at: 2864 3389 2864
Size: 525 5920
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6248
AflIII A'CryG_T
Cuts at: 1203 5344 6321 1203
Size: 4141 977 1327
Fragments arranged by size:
4141 1327 977
AgeI A'CCGG_T
Cuts at: 2014 5276 2014
Size: 3262 3183
AhdI GACnn_n'nnGTC
Cuts at: 5174 5174
Size: 6445
AleI CACnn'nnGTG
Cuts at: 1166 2140 2675 1166
Size: 974 535 4936
Fragments arranged by size:
4936 974 535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2954 3211 1184
Size: 1770 257 4418
Fragments arranged by size:
4418 1770 257
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 2543
Size: 32 6413
AlwNI CAG_nnn'CTG
Cuts at: 2458 2806 2830 3004 3169 3736 4188 5760 2458
Size: 348 24 174 165 567 452 1572 3143
Fragments arranged by size:
3143 1572 567 452 348 174 165 24
ApaI G_GGCC'C
Cuts at: 2527 2527
Size: 6445
ApaLI G'TGCA_C
Cuts at: 2314 2645 2912 4502 5658 2314
Size: 331 267 1590 1156 3101
Fragments arranged by size:
3101 1590 1156 331 267
ApoI r'AATT_y
Cuts at: 1493 3754 3980 4132 6241 1493
Size: 2261 226 152 2109 1697
Fragments arranged by size:
2261 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4999 160
Size: 872 3967 1606
Fragments arranged by size:
3967 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
AvaI C'yCGr_G
Cuts at: 1870 1943 2486 3770 1870
Size: 73 543 1284 4545
Fragments arranged by size:
4545 1284 543 73
BamHI G'GATC_C
Cuts at: 3775 4142 3775
Size: 367 6078
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2176 2669 2902 3488 3554
Size: 325 217 627 389 493 233 586 66
Cuts at: 3554 3578 3766 5221 618
Size: 24 188 1455 1842
Fragments arranged by size:
1842 1455 627 586 493 389 325 233
217 188 66 24
BanII G_rGCy'C
Cuts at: 1391 2197 2527 2595 2745 3159 3335 3726 3764
Size: 806 330 68 150 414 176 391 38
Cuts at: 3764 1391
Size: 4072
Fragments arranged by size:
4072 806 414 391 330 176 150 68
38
BbeI G_GCGC'C
Cuts at: 2180 3492 3558 2180
Size: 1312 66 5067
Fragments arranged by size:
5067 1312 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5474
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4636 4670 1903
Size: 34 2699 34 3678
Fragments arranged by size:
3678 2699 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4337 5547 4337
Size: 1210 5235
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6445
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2408 2636 3242
Size: 107 122 71 631 605 736 228 606
Cuts at: 3242 3404 3552 3677 5056 136
Size: 162 148 125 1379 1525
Fragments arranged by size:
1525 1379 736 631 606 605 228 162
148 125 122 107 71
BglII A'GATC_T
Cuts at: 2292 6440 2292
Size: 4148 2297
BlpI GC'TnA_GC
Cuts at: 3048 3862 3048
Size: 814 5631
Bme1580I G_kGCm'C
Cuts at: 1790 2318 2527 2649 2907 2916 3711 4506 5662
Size: 528 209 122 258 9 795 795 1156
Cuts at: 5662 1790
Size: 2573
Fragments arranged by size:
2573 1156 795 795 528 258 209 122
9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6445
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2926 3269 4176
Size: 416 53 83 399 24 1509 343 907
Cuts at: 4176 5134 442
Size: 958 1753
Fragments arranged by size:
1753 1509 958 907 416 399 343 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2110 2759 2783 3191 3559 3665 3758 5105
Size: 871 649 24 408 368 106 93 1347
Cuts at: 5105 1239
Size: 2579
Fragments arranged by size:
2579 1347 871 649 408 368 106 93
24
Bpu10I CC'TnA_GC
Cuts at: 3413 3638 3698 3413
Size: 225 60 6160
Fragments arranged by size:
6160 225 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2585 3605 4570 5435 5733 2513
Size: 72 1020 965 865 298 3225
Fragments arranged by size:
3225 1020 965 865 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2277 3641 3734 5108 882
Size: 617 778 1364 93 1374 2219
Fragments arranged by size:
2219 1374 1364 778 617 93
BsaAI yAC'GTr
Cuts at: 493 1204 2440 3561 4236 493
Size: 711 1236 1121 675 2702
Fragments arranged by size:
2702 1236 1121 711 675
BsaBI GATnn'nnATC
Cuts at: 6439 6439
Size: 6445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2177 3231 3489 3555
Size: 53 83 186 936 644 1054 258 66
Cuts at: 3555 4252 4634 5269 275
Size: 697 382 635 1451
Fragments arranged by size:
1451 1054 936 697 644 635 382 258
186 83 66 53
BsaWI w'CCGG_w
Cuts at: 2014 4878 5276 5550 5697 6108 2014
Size: 2864 398 274 147 411 2351
Fragments arranged by size:
2864 2351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2357 2387 1178
Size: 30 165 30 954 30 5236
Fragments arranged by size:
5236 954 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2242 3188 3734 1186
Size: 991 65 946 546 3897
Fragments arranged by size:
3897 991 946 546 65
BseYI C'CCAG_C
Cuts at: 2446 2774 2790 3712 5648 6144 2446
Size: 328 16 922 1936 496 2747
Fragments arranged by size:
2747 1936 922 496 328 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2039 2645 2711 1163
Size: 573 297 6 606 66 4897
Fragments arranged by size:
4897 606 573 297 66 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2015 3895 4656 5684 1540
Size: 275 186 14 1880 761 1028 2301
Fragments arranged by size:
2301 1880 1028 761 275 186 14
BsiHKAI G_wGCw'C
Cuts at: 2157 2318 2465 2595 2649 2745 2916 2943 3414
Size: 161 147 130 54 96 171 27 471
Cuts at: 3414 3688 3726 4506 4591 5662 2157
Size: 274 38 780 85 1071 2940
Fragments arranged by size:
2940 1071 780 471 274 171 161 147
130 96 85 54 38 27
BsmI GAATG_Cn'
Cuts at: 3956 4049 3956
Size: 93 6352
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2197 2318 2465 2527 2595 2649
Size: 399 367 40 121 147 62 68 54
Cuts at: 2649 2745 2907 2916 2943 3159 3335 3414 3688
Size: 96 162 9 27 216 176 79 274
Cuts at: 3688 3711 3726 3764 4506 4591 5662 1391
Size: 23 15 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 367 274 216 176
162 147 121 96 85 79 68 62
54 40 38 27 23 15 9
BspEI T'CCGG_A
Cuts at: 6108 6108
Size: 6445
BspHI T'CATG_A
Cuts at: 4334 6064 4334
Size: 1730 4715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 2484 2712 3800 844
Size: 1415 225 228 1088 3489
Fragments arranged by size:
3489 1415 1088 228 225
BsrBI CCG'CTC
Cuts at: 1394 1823 4332 1394
Size: 429 2509 3507
Fragments arranged by size:
3507 2509 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4940 5114 65
Size: 1183 3692 174 1396
Fragments arranged by size:
3692 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2960 3065 5089 5276 1700
Size: 110 204 946 105 2024 187 2869
Fragments arranged by size:
2869 2024 946 204 187 110 105
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6445
BssHII G'CGCG_C
Cuts at: 1925 3466 1925
Size: 1541 4904
BssSI C'ACGA_G
Cuts at: 2240 2588 3681 3704 4505 5517 2240
Size: 348 1093 23 801 1012 3168
Fragments arranged by size:
3168 1093 1012 801 348 23
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6445
BstBI TT'CG_AA
Cuts at: 5249 5249
Size: 6445
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6445
BstXI CCAn_nnnn'nTGG
Cuts at: 3439 3439
Size: 6445
BstYI r'GATC_y
Cuts at: 1972 2292 3775 4142 4527 4544 5996 6082 6094
Size: 320 1483 367 385 17 1452 86 12
Cuts at: 6094 6440 1972
Size: 346 1977
Fragments arranged by size:
1977 1483 1452 385 367 346 320 86
17 12
BstZ17I GTA'TAC
Cuts at: 2493 2493
Size: 6445
BtgI C'CryG_G
Cuts at: 513 1065 2163 3151 3425 3495 513
Size: 552 1098 988 274 70 3463
Fragments arranged by size:
3463 1098 988 552 274 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5706
Fragments arranged by size:
5706 683 56
BtsI GCAGTG_nn'
Cuts at: 2943 3015 3961 4755 4775 2943
Size: 72 946 794 20 4613
Fragments arranged by size:
4613 946 794 72 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6410
DraI TTT'AAA
Cuts at: 3749 3749
Size: 6445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5452 809
Size: 507 4136 1802
Fragments arranged by size:
4136 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2069 3654 3892
Size: 54 1425 50 275 18 239 1585 238
Cuts at: 3892 4781 6410 8
Size: 889 1629 43
Fragments arranged by size:
1629 1585 1425 889 275 239 238 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3892 1537
Size: 275 2080 4090
Fragments arranged by size:
4090 2080 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 2337 2600 3348 4375 1353
Size: 866 118 263 748 1027 3423
Fragments arranged by size:
3423 1027 866 748 263 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5032 5406 5552 387
Size: 1064 138 3443 374 146 1280
Fragments arranged by size:
3443 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2593 2743 3724 2593
Size: 150 981 5314
Fragments arranged by size:
5314 981 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2074 2110 2297 2759 2770 2783 2828
Size: 393 442 36 187 462 11 13 45
Cuts at: 2828 3191 3559 3665 3758 4508 5105 5892 6160
Size: 363 368 106 93 750 597 787 268
Cuts at: 6160 1239
Size: 1524
Fragments arranged by size:
1524 787 750 597 462 442 393 368
363 268 187 106 93 45 36 13
11
EcoNI CCTnn'n_nnAGG
Cuts at: 2639 2822 2639
Size: 183 6262
EcoO109I rG'GnC_Cy
Cuts at: 2107 2431 2523 2524 2578 2794 2803 2814 2107
Size: 324 92 1 54 216 9 11 5738
Fragments arranged by size:
5738 324 216 92 54 11 9 1
EcoRI G'AATT_C
Cuts at: 3754 4132 3754
Size: 378 6067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 6445
FspI TGC'GCA
Cuts at: 3449 4951 3449
Size: 1502 4943
HaeII r_GCGC'y
Cuts at: 1678 2180 2263 2866 3307 3391 3492 3558 3638
Size: 502 83 603 441 84 101 66 80
Cuts at: 3638 5592 1678
Size: 1954 2531
Fragments arranged by size:
2531 1954 603 502 441 101 84 83
80 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2357 2389 5086 5118 5160
Size: 32 135 32 785 32 2697 32 42
Cuts at: 5160 5192 1373
Size: 32 2626
Fragments arranged by size:
2697 2626 785 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2974 3789 4035 669
Size: 988 281 1036 815 246 3079
Fragments arranged by size:
3079 1036 988 815 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6445
HpaI GTT'AAC
Cuts at: 4035 4035
Size: 6445
KasI G'GCGC_C
Cuts at: 2176 3488 3554 2176
Size: 1312 66 5067
Fragments arranged by size:
5067 1312 66
KpnI G_GTAC'C
Cuts at: 3770 3770
Size: 6445
MfeI C'AATT_G
Cuts at: 2619 4044 2619
Size: 1425 5020
MluI A'CGCG_T
Cuts at: 6321 6321
Size: 6445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5559 5743 1305
Size: 4254 184 2007
Fragments arranged by size:
4254 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3656 10
Size: 54 1425 343 1824 2799
Fragments arranged by size:
2799 1824 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2140 2675 3407 4403 4762 4921 518
Size: 648 974 535 732 996 359 159 2042
Fragments arranged by size:
2042 996 974 732 648 535 359 159
MspA1I CmG'CkG
Cuts at: 1606 2165 2389 2790 3202 3325 3427 3716 3861
Size: 559 224 401 412 123 102 289 145
Cuts at: 3861 4538 5686 5931 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 559 412 401 289 245
224 145 123 102
NaeI GCC'GGC
Cuts at: 2962 2962
Size: 6445
NarI GG'CG_CC
Cuts at: 2177 3489 3555 2177
Size: 1312 66 5067
Fragments arranged by size:
5067 1312 66
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5893
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6445
NgoMIV G'CCGG_C
Cuts at: 2960 2960
Size: 6445
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6445
NotI GC'GGCC_GC
Cuts at: 3892 3892
Size: 6445
NspI r_CATG'y
Cuts at: 1132 1229 3803 5348 1132
Size: 97 2574 1545 2229
Fragments arranged by size:
2574 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6445
PciI A'CATG_T
Cuts at: 5344 5344
Size: 6445
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2548 2737 2830 3004 3092 3169 3736 1108
Size: 1440 189 93 174 88 77 567 3817
Fragments arranged by size:
3817 1440 567 189 174 93 88 77
PfoI T'CCnGG_A
Cuts at: 2541 2598 2799 3168 2541
Size: 57 201 369 5818
Fragments arranged by size:
5818 369 201 57
PmeI GTTT'AAAC
Cuts at: 3749 3749
Size: 6445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4514 4546 6027 6059 4514
Size: 32 1481 32 4900
Fragments arranged by size:
4900 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2794 2803 2814 2794
Size: 9 11 6425
Fragments arranged by size:
6425 11 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6445
PsiI TTA'TAA
Cuts at: 4015 4015
Size: 6445
PspOMI G'GGCC_C
Cuts at: 2523 2523
Size: 6445
PstI C_TGCA'G
Cuts at: 830 2292 3797 830
Size: 1462 1505 3478
Fragments arranged by size:
3478 1505 1462
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6445
PvuII CAG'CTG
Cuts at: 2790 3202 3325 2790
Size: 412 123 5910
Fragments arranged by size:
5910 412 123
SacI G_AGCT'C
Cuts at: 2595 2745 3726 2595
Size: 150 981 5314
Fragments arranged by size:
5314 981 150
SacII CC_GC'GG
Cuts at: 2166 3428 2166
Size: 1262 5183
SalI G'TCGA_C
Cuts at: 1936 3787 1936
Size: 1851 4594
SanDI GG'GwC_CC
Cuts at: 2814 2814
Size: 6445
SapI GCTCTTCn'nnn_
Cuts at: 2600 2600
Size: 6445
SbfI CC_TGCA'GG
Cuts at: 3797 3797
Size: 6445
ScaI AGT'ACT
Cuts at: 4693 6405 4693
Size: 1712 4733
SfcI C'TryA_G
Cuts at: 826 1045 2288 3793 4928 5609 5800 826
Size: 219 1243 1505 1135 681 191 1471
Fragments arranged by size:
1505 1471 1243 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2178 3490 3556 2178
Size: 1312 66 5067
Fragments arranged by size:
5067 1312 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6445
SmaI CCC'GGG
Cuts at: 1872 3772 1872
Size: 1900 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2528 2564 3620 4549 5450 5712
Size: 197 926 585 36 1056 929 901 262
Cuts at: 5712 820
Size: 1553
Fragments arranged by size:
1553 1056 929 926 901 585 262 197
36
SnaBI TAC'GTA
Cuts at: 493 4236 493
Size: 3743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6445
SphI G_CATG'C
Cuts at: 1132 3803 1132
Size: 2671 3774
SspI AAT'ATT
Cuts at: 5 52 4369 5
Size: 47 4317 2081
Fragments arranged by size:
4317 2081 47
StuI AGG'CCT
Cuts at: 1517 3041 1517
Size: 1524 4921
StyI C'CwwG_G
Cuts at: 513 1065 2117 3600 3651 3884 513
Size: 552 1052 1483 51 233 3074
Fragments arranged by size:
3074 1483 1052 552 233 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4472 4489 4642 4827 5272 325
Size: 1255 2892 17 153 185 445 1498
Fragments arranged by size:
2892 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2694 4691 6403
Size: 274 80 33 51 1443 717 1997 1712
Cuts at: 6403 96
Size: 138
Fragments arranged by size:
1997 1712 1443 717 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2294 2326 6405 6437 2294
Size: 32 4079 32 2302
Fragments arranged by size:
4079 2302 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6445
XbaI T'CTAG_A
Cuts at: 3781 3781
Size: 6445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2167
Size: 6445
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6445
XmaI C'CCGG_G
Cuts at: 1870 3770 1870
Size: 1900 4545
XmnI GAAnn'nnTTC
Cuts at: 4574 4574
Size: 6445
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4253 276
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI
Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SexAI SfiI SrfI SwaI