Restriction map for
pFN21AE0980
(Circular) (Six-base) MAPSORT of: pFN21AE0980.seq Check: 6253 from: 1 to: 6439
pFN21AE0980 6439 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4249 278
Size: 53 83 186 936 2713 2468
Fragments arranged by size:
2713 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2221 3122 3782 1937
Size: 284 901 660 4594
Fragments arranged by size:
4594 901 660 284
Acc65I G'GTAC_C
Cuts at: 3607 3760 3607
Size: 153 6286
AclI AA'CG_TT
Cuts at: 1563 4566 4939 1563
Size: 3003 373 3063
Fragments arranged by size:
3063 3003 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2276 2852 4502 5886 6154 1632
Size: 644 576 1650 1384 268 1917
Fragments arranged by size:
1917 1650 1384 644 576 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6242
AflIII A'CryG_T
Cuts at: 1203 5338 6315 1203
Size: 4135 977 1327
Fragments arranged by size:
4135 1327 977
AgeI A'CCGG_T
Cuts at: 5270 5270
Size: 6439
AhdI GACnn_n'nnGTC
Cuts at: 5168 5168
Size: 6439
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2057 1184
Size: 873 5566
AlwNI CAG_nnn'CTG
Cuts at: 2092 2782 3476 4182 5754 2092
Size: 690 694 706 1572 2777
Fragments arranged by size:
2777 1572 706 694 690
ApaLI G'TGCA_C
Cuts at: 4496 5652 4496
Size: 1156 5283
ApoI r'AATT_y
Cuts at: 1493 2133 2264 2926 3676 3748 3974 4126 6235
Size: 640 131 662 750 72 226 152 2109
Cuts at: 6235 1493
Size: 1697
Fragments arranged by size:
2109 1697 750 662 640 226 152 131
72
AseI AT'TA_AT
Cuts at: 160 1032 4993 160
Size: 872 3961 1606
Fragments arranged by size:
3961 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
AvaI C'yCGr_G
Cuts at: 1870 1943 3764 1870
Size: 73 1821 4545
Fragments arranged by size:
4545 1821 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2819 2852 3591 3624 2819
Size: 33 739 33 5634
Fragments arranged by size:
5634 739 33 33
BamHI G'GATC_C
Cuts at: 3769 4136 3769
Size: 367 6072
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 3064 3607 3760 5215
Size: 325 217 627 269 1008 543 153 1455
Cuts at: 5215 618
Size: 1842
Fragments arranged by size:
1842 1455 1008 627 543 325 269 217
153
BanII G_rGCy'C
Cuts at: 1391 3343 3758 1391
Size: 1952 415 4072
Fragments arranged by size:
4072 1952 415
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 3334 928
Size: 971 383 1052 4033
Fragments arranged by size:
4033 1052 971 383
BbvCI CC'TCA_GC
Cuts at: 2665 2665
Size: 6439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3593 3627 4630 4664 1903
Size: 34 1656 34 1003 34 3678
Fragments arranged by size:
3678 1656 1003 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4331 5541 4331
Size: 1210 5229
BclI T'GATC_A
Cuts at: 1548 2310 3715 1548
Size: 762 1405 4272
Fragments arranged by size:
4272 1405 762
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 5050 136
Size: 107 122 71 631 605 406 2972 1525
Fragments arranged by size:
2972 1525 631 605 406 122 107 71
BglII A'GATC_T
Cuts at: 6434 6434
Size: 6439
BlpI GC'TnA_GC
Cuts at: 3662 3856 3662
Size: 194 6245
Bme1580I G_kGCm'C
Cuts at: 1790 4500 5656 1790
Size: 2710 1156 2573
Fragments arranged by size:
2710 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3498 1318
Size: 2180 4259
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4170 5128 442
Size: 416 53 83 399 24 2753 958 1753
Fragments arranged by size:
2753 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2774 5099 1239
Size: 1535 2325 2579
Fragments arranged by size:
2579 2325 1535
Bpu10I CC'TnA_GC
Cuts at: 2665 2665
Size: 6439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2942 4564 5429 5727 2942
Size: 1622 865 298 3654
Fragments arranged by size:
3654 1622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5102 882
Size: 617 3603 2219
Fragments arranged by size:
3603 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4230 493
Size: 711 3026 2702
Fragments arranged by size:
3026 2702 711
BsaBI GATnn'nnATC
Cuts at: 6433 6433
Size: 6439
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4246 4628 5263 275
Size: 53 83 186 936 2713 382 635 1451
Fragments arranged by size:
2713 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4872 5270 5544 5691 6102 4872
Size: 398 274 147 411 5209
Fragments arranged by size:
5209 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3440 3470 1178
Size: 30 165 30 2037 30 4147
Fragments arranged by size:
4147 2037 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3377 3546 1186
Size: 2191 169 4079
Fragments arranged by size:
4079 2191 169
BseYI C'CCAG_C
Cuts at: 2541 3615 5642 6138 2541
Size: 1074 2027 496 2842
Fragments arranged by size:
2842 2027 1074 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5866
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3053 3889 4650 5678 1540
Size: 275 186 1052 836 761 1028 2301
Fragments arranged by size:
2301 1052 1028 836 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3343 3602 3727 4500 4585 5656 3343
Size: 259 125 773 85 1071 4126
Fragments arranged by size:
4126 1071 773 259 125 85
BsmI GAATG_Cn'
Cuts at: 3436 3950 4043 3436
Size: 514 93 5832
Fragments arranged by size:
5832 514 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3630 3630
Size: 6439
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3343 3602 3727 3758 4500 4585 5656
Size: 399 1553 259 125 31 742 85 1071
Cuts at: 5656 1391
Size: 2174
Fragments arranged by size:
2174 1553 1071 742 399 259 125 85
31
BspEI T'CCGG_A
Cuts at: 6102 6102
Size: 6439
BspHI T'CATG_A
Cuts at: 2842 4328 6058 2842
Size: 1486 1730 3223
Fragments arranged by size:
3223 1730 1486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3619 3794 844
Size: 2775 175 3489
Fragments arranged by size:
3489 2775 175
BsrBI CCG'CTC
Cuts at: 1394 1823 4326 1394
Size: 429 2503 3507
Fragments arranged by size:
3507 2503 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4934 5108 65
Size: 1183 3686 174 1396
Fragments arranged by size:
3686 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 5083 5270 1700
Size: 110 220 3053 187 2869
Fragments arranged by size:
3053 2869 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6439
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6439
BssSI C'ACGA_G
Cuts at: 4499 5511 4499
Size: 1012 5427
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6439
BstBI TT'CG_AA
Cuts at: 5243 5243
Size: 6439
BstEII G'GTnAC_C
Cuts at: 1184 2638 1184
Size: 1454 4985
BstXI CCAn_nnnn'nTGG
Cuts at: 3017 3103 3017
Size: 86 6353
BstYI r'GATC_y
Cuts at: 1972 2362 2710 3234 3356 3473 3769 4136 4521
Size: 390 348 524 122 117 296 367 385
Cuts at: 4521 4538 5990 6076 6088 6434 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 524 390 385 367 348 346
296 122 117 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3096 3352 513
Size: 552 939 1092 256 3600
Fragments arranged by size:
3600 1092 939 552 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3210 3492 487
Size: 683 56 1984 282 3434
Fragments arranged by size:
3434 1984 683 282 56
BtsI GCAGTG_nn'
Cuts at: 3010 3955 4749 4769 3010
Size: 945 794 20 4680
Fragments arranged by size:
4680 945 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6439
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6404
DraI TTT'AAA
Cuts at: 3743 3743
Size: 6439
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5446 809
Size: 507 4130 1802
Fragments arranged by size:
4130 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3019 3108 3886
Size: 54 1425 50 275 18 1189 89 778
Cuts at: 3886 4775 6404 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1189 889 778 275 89 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3886 1537
Size: 275 2074 4090
Fragments arranged by size:
4090 2074 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2232 3281 3426 4369 1353
Size: 879 1049 145 943 3423
Fragments arranged by size:
3423 1049 943 879 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5026 5400 5546 387
Size: 1064 138 3437 374 146 1280
Fragments arranged by size:
3437 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3341 3341
Size: 6439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2774 2852 4502 5099 5886 6154
Size: 393 644 498 78 1650 597 787 268
Cuts at: 6154 1239
Size: 1524
Fragments arranged by size:
1650 1524 787 644 597 498 393 268
78
EcoO109I rG'GnC_Cy
Cuts at: 2779 3654 2779
Size: 875 5564
EcoRI G'AATT_C
Cuts at: 2264 2926 3748 4126 2264
Size: 662 822 378 4577
Fragments arranged by size:
4577 822 662 378
EcoRV GAT'ATC
Cuts at: 3537 3537
Size: 6439
FspI TGC'GCA
Cuts at: 4945 4945
Size: 6439
HaeII r_GCGC'y
Cuts at: 1678 5586 1678
Size: 3908 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2497 2529 3325 3357 3376
Size: 32 135 32 925 32 796 32 19
Cuts at: 3376 3408 5080 5112 5154 5186 1373
Size: 32 1672 32 42 32 2626
Fragments arranged by size:
2626 1672 925 796 135 42 32 32
32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 3783 4029 669
Size: 988 281 1845 246 3079
Fragments arranged by size:
3079 1845 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6439
HpaI GTT'AAC
Cuts at: 4029 4029
Size: 6439
KpnI G_GTAC'C
Cuts at: 3611 3764 3611
Size: 153 6286
MfeI C'AATT_G
Cuts at: 4038 4038
Size: 6439
MluI A'CGCG_T
Cuts at: 6315 6315
Size: 6439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2453 3096 5553 5737 1305
Size: 1148 643 2457 184 2007
Fragments arranged by size:
2457 2007 1148 643 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3021 10
Size: 54 1425 343 1189 3428
Fragments arranged by size:
3428 1425 1189 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3101 4397 4756 4915 518
Size: 648 1935 1296 359 159 2042
Fragments arranged by size:
2042 1935 1296 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2151 3855 4532 5680 5925 1606
Size: 456 89 1704 677 1148 245 2120
Fragments arranged by size:
2120 1704 1148 677 456 245 89
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 6439
NcoI C'CATG_G
Cuts at: 513 1065 2004 3096 3352 513
Size: 552 939 1092 256 3600
Fragments arranged by size:
3600 1092 939 552 256
NdeI CA'TA_TG
Cuts at: 387 2806 387
Size: 2419 4020
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 6439
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6439
NotI GC'GGCC_GC
Cuts at: 3886 3886
Size: 6439
NspI r_CATG'y
Cuts at: 1132 1229 3157 3797 5342 1132
Size: 97 1928 640 1545 2229
Fragments arranged by size:
2229 1928 1545 640 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6439
PciI A'CATG_T
Cuts at: 5338 5338
Size: 6439
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6439
PmeI GTTT'AAAC
Cuts at: 3743 3743
Size: 6439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4508 4540 6021 6053 4508
Size: 32 1481 32 4894
Fragments arranged by size:
4894 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2904 1613
Size: 1291 5148
PsiI TTA'TAA
Cuts at: 4009 4009
Size: 6439
PstI C_TGCA'G
Cuts at: 830 2097 3791 830
Size: 1267 1694 3478
Fragments arranged by size:
3478 1694 1267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6439
PvuII CAG'CTG
Cuts at: 2151 2151
Size: 6439
SacI G_AGCT'C
Cuts at: 3343 3343
Size: 6439
SalI G'TCGA_C
Cuts at: 1936 3781 1936
Size: 1845 4594
SapI GCTCTTCn'nnn_
Cuts at: 3426 3426
Size: 6439
SbfI CC_TGCA'GG
Cuts at: 3791 3791
Size: 6439
ScaI AGT'ACT
Cuts at: 2756 4687 6399 2756
Size: 1931 1712 2796
Fragments arranged by size:
2796 1931 1712
SfcI C'TryA_G
Cuts at: 826 1045 2093 3787 4922 5603 5794 826
Size: 219 1048 1694 1135 681 191 1471
Fragments arranged by size:
1694 1471 1135 1048 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6439
SmaI CCC'GGG
Cuts at: 1872 3766 1872
Size: 1894 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2921 4543 5444 5706 820
Size: 197 926 978 1622 901 262 1553
Fragments arranged by size:
1622 1553 978 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4230 493
Size: 3737 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6439
SphI G_CATG'C
Cuts at: 1132 3157 3797 1132
Size: 2025 640 3774
Fragments arranged by size:
3774 2025 640
SspI AAT'ATT
Cuts at: 5 52 2492 2820 4363 5
Size: 47 2440 328 1543 2081
Fragments arranged by size:
2440 2081 1543 328 47
StuI AGG'CCT
Cuts at: 1517 2214 1517
Size: 697 5742
StyI C'CwwG_G
Cuts at: 513 1065 2004 2551 3096 3352 3878 513
Size: 552 939 547 545 256 526 3074
Fragments arranged by size:
3074 939 552 547 545 526 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4466 4483 4636 4821 5266 325
Size: 1255 2886 17 153 185 445 1498
Fragments arranged by size:
2886 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2754 4685 6397
Size: 274 80 33 51 1443 777 1931 1712
Cuts at: 6397 96
Size: 138
Fragments arranged by size:
1931 1712 1443 777 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2124 2156 6399 6431 2124
Size: 32 4243 32 2132
Fragments arranged by size:
4243 2132 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6439
XbaI T'CTAG_A
Cuts at: 3775 3775
Size: 6439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3103 2011
Size: 1092 5347
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6439
XmaI C'CCGG_G
Cuts at: 1870 3764 1870
Size: 1894 4545
XmnI GAAnn'nnTTC
Cuts at: 2492 4568 2492
Size: 2076 4363
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4247 276
Size: 53 83 186 936 2713 2468
Fragments arranged by size:
2713 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI