Restriction map for
pFN21AE0975
(Circular) (Six-base) MAPSORT of: pFN21AE0975.seq Check: 242 from: 1 to: 6337
pFN21AE0975 6337 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3114 3618 3114
Size: 504 5833
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2340 4147 278
Size: 53 83 186 936 804 1807 2468
Fragments arranged by size:
2468 1807 936 804 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3680 1937
Size: 1743 4594
Acc65I G'GTAC_C
Cuts at: 2550 3658 2550
Size: 1108 5229
AclI AA'CG_TT
Cuts at: 1563 3231 4464 4837 1563
Size: 1668 1233 373 3063
Fragments arranged by size:
3063 1668 1233 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4400 5784 6052 1632
Size: 2768 1384 268 1917
Fragments arranged by size:
2768 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6140
AflIII A'CryG_T
Cuts at: 1203 2604 5236 6213 1203
Size: 1401 2632 977 1327
Fragments arranged by size:
2632 1401 1327 977
AgeI A'CCGG_T
Cuts at: 5168 5168
Size: 6337
AhdI GACnn_n'nnGTC
Cuts at: 5066 5066
Size: 6337
AleI CACnn'nnGTG
Cuts at: 1166 2015 1166
Size: 849 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6337
AlwNI CAG_nnn'CTG
Cuts at: 2368 2797 2899 3406 4080 5652 2368
Size: 429 102 507 674 1572 3053
Fragments arranged by size:
3053 1572 674 507 429 102
ApaI G_GGCC'C
Cuts at: 3077 3077
Size: 6337
ApaLI G'TGCA_C
Cuts at: 2008 2405 4394 5550 2008
Size: 397 1989 1156 2795
Fragments arranged by size:
2795 1989 1156 397
ApoI r'AATT_y
Cuts at: 1493 3646 3872 4024 6133 1493
Size: 2153 226 152 2109 1697
Fragments arranged by size:
2153 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4891 160
Size: 872 3859 1606
Fragments arranged by size:
3859 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6337
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2392 2970 3061 3662 1870
Size: 73 117 332 578 91 601 4545
Fragments arranged by size:
4545 601 578 332 117 91 73
AvrII C'CTAG_G
Cuts at: 3274 3274
Size: 6337
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2534 2567 2534
Size: 33 6304
BamHI G'GATC_C
Cuts at: 3667 4034 3667
Size: 367 5970
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2550 3056 3530 3542 3627
Size: 325 217 627 763 506 474 12 85
Cuts at: 3627 3658 5113 618
Size: 31 1455 1842
Fragments arranged by size:
1842 1455 763 627 506 474 325 217
85 31 12
BanII G_rGCy'C
Cuts at: 1391 3077 3656 1391
Size: 1686 579 4072
Fragments arranged by size:
4072 1686 579
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5366
BbvCI CC'TCA_GC
Cuts at: 3129 3129
Size: 6337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4528 4562 1903
Size: 34 2591 34 3678
Fragments arranged by size:
3678 2591 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2633 4229 5439 2633
Size: 1596 1210 3531
Fragments arranged by size:
3531 1596 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6337
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3401 4948 136
Size: 107 122 71 631 605 1729 1547 1525
Fragments arranged by size:
1729 1547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6332 6332
Size: 6337
BlpI GC'TnA_GC
Cuts at: 3754 3754
Size: 6337
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2400 2409 2950 2977 3077 3535 3630
Size: 222 388 9 541 27 100 458 95
Cuts at: 3630 4398 5554 1790
Size: 768 1156 2573
Fragments arranged by size:
2573 1156 768 541 458 388 222 100
95 27 9
BmgBI CAC'GTC
Cuts at: 1318 2239 2667 1318
Size: 921 428 4988
Fragments arranged by size:
4988 921 428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3611 4068 5026
Size: 416 53 83 399 24 2194 457 958
Cuts at: 5026 442
Size: 1753
Fragments arranged by size:
2194 1753 958 457 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6337
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 3103
Size: 6337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2705 2717 3056 3641 4997 1239
Size: 941 525 12 339 585 1356 2579
Fragments arranged by size:
2579 1356 941 585 525 339 12
Bpu10I CC'TnA_GC
Cuts at: 2743 3129 3387 2743
Size: 386 258 5693
Fragments arranged by size:
5693 386 258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4462 5327 5625 4462
Size: 865 298 5174
Fragments arranged by size:
5174 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2641 2693 5000 882
Size: 617 1142 52 2307 2219
Fragments arranged by size:
2307 2219 1142 617 52
BsaAI yAC'GTr
Cuts at: 493 1204 2344 2536 2914 4128 493
Size: 711 1140 192 378 1214 2702
Fragments arranged by size:
2702 1214 1140 711 378 192
BsaBI GATnn'nnATC
Cuts at: 6331 6331
Size: 6337
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2337 4144 4526 5161
Size: 53 83 186 936 804 1807 382 635
Cuts at: 5161 275
Size: 1451
Fragments arranged by size:
1807 1451 936 804 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2034 2100 2313 2560 3481 4770 5168 5442 5589
Size: 66 213 247 921 1289 398 274 147
Cuts at: 5589 6000 2034
Size: 411 2371
Fragments arranged by size:
2371 1289 921 411 398 274 247 213
147 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2095 2125 2333 2363 1178
Size: 30 165 30 692 30 208 30 5152
Fragments arranged by size:
5152 692 208 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2735 1186
Size: 997 552 4788
Fragments arranged by size:
4788 997 552
BseYI C'CCAG_C
Cuts at: 2773 2933 5540 6036 2773
Size: 160 2607 496 3074
Fragments arranged by size:
3074 2607 496 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2434 2750 2936 3092 3173 3240 1163
Size: 573 698 316 186 156 81 67 4260
Fragments arranged by size:
4260 698 573 316 186 156 81 67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 3787 4548 5576 1540
Size: 275 186 1498 288 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 288 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2409 2436 2560 3207 4398 4483 5554 2012
Size: 397 27 124 647 1191 85 1071 2795
Fragments arranged by size:
2795 1191 1071 647 397 124 85 27
BsmI GAATG_Cn'
Cuts at: 3848 3941 3848
Size: 93 6244
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2400 2409 2436 2560 2950 2977
Size: 399 222 388 9 27 124 390 27
Cuts at: 2977 3077 3207 3535 3630 3656 4398 4483 5554
Size: 100 130 328 95 26 742 85 1071
Cuts at: 5554 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 390 388 328 222
130 124 100 95 85 27 27 26
9
BspEI T'CCGG_A
Cuts at: 2034 2313 3481 6000 2034
Size: 279 1168 2519 2371
Fragments arranged by size:
2519 2371 1168 279
BspHI T'CATG_A
Cuts at: 4226 5956 4226
Size: 1730 4607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3114 3618 3692 844
Size: 2270 504 74 3489
Fragments arranged by size:
3489 2270 504 74
BsrBI CCG'CTC
Cuts at: 1394 1823 2484 3251 4224 1394
Size: 429 661 767 973 3507
Fragments arranged by size:
3507 973 767 661 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4832 5006 65
Size: 1183 3584 174 1396
Fragments arranged by size:
3584 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4981 5168 1700
Size: 110 3171 187 2869
Fragments arranged by size:
3171 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6337
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6337
BssSI C'ACGA_G
Cuts at: 2949 3206 4397 5409 2949
Size: 257 1191 1012 3877
Fragments arranged by size:
3877 1191 1012 257
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2404 794
Size: 1610 4727
BstBI TT'CG_AA
Cuts at: 5141 5141
Size: 6337
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6337
BstXI CCAn_nnnn'nTGG
Cuts at: 2204 2204
Size: 6337
BstYI r'GATC_y
Cuts at: 1972 3667 4034 4419 4436 5888 5974 5986 6332
Size: 1695 367 385 17 1452 86 12 346
Cuts at: 6332 1972
Size: 1977
Fragments arranged by size:
1977 1695 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2502 2511 2846 2502
Size: 9 335 5993
Fragments arranged by size:
5993 335 9
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5785
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5598
Fragments arranged by size:
5598 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 2897 2926 3853 4647 4667 2100
Size: 797 29 927 794 20 3770
Fragments arranged by size:
3770 927 797 794 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6337
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6302
DraI TTT'AAA
Cuts at: 3641 3641
Size: 6337
DraIII CAC_nnn'GTG
Cuts at: 2404 3634 2404
Size: 1230 5107
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5344 809
Size: 507 4028 1802
Fragments arranged by size:
4028 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2538 2783
Size: 54 1425 50 275 18 291 417 245
Cuts at: 2783 3393 3447 3496 3784 4673 6302 8
Size: 610 54 49 288 889 1629 43
Fragments arranged by size:
1629 1425 889 610 417 291 288 275
245 54 54 50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 3784 1537
Size: 275 1684 288 4090
Fragments arranged by size:
4090 1684 288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2581 4267 1353
Size: 1228 1686 3423
Fragments arranged by size:
3423 1686 1228
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4924 5298 5444 387
Size: 1064 138 3335 374 146 1280
Fragments arranged by size:
3335 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2705 2717 3056 3641 4400 4997
Size: 393 548 525 12 339 585 759 597
Cuts at: 4997 5784 6052 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 759 597 585 548 525 393
339 268 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 2501 3620 2330
Size: 171 1119 5047
Fragments arranged by size:
5047 1119 171
EcoO109I rG'GnC_Cy
Cuts at: 2070 2507 2725 2778 3073 3158 3171 3291 3403
Size: 437 218 53 295 85 13 120 112
Cuts at: 3403 3463 2070
Size: 60 4944
Fragments arranged by size:
4944 437 295 218 120 112 85 60
53 13
EcoRI G'AATT_C
Cuts at: 3646 4024 3646
Size: 378 5959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 6337
FspI TGC'GCA
Cuts at: 4843 4843
Size: 6337
HaeII r_GCGC'y
Cuts at: 1678 3314 5484 1678
Size: 1636 2170 2531
Fragments arranged by size:
2531 2170 1636
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2031 2063 3194 3226 4978
Size: 32 135 32 459 32 1131 32 1752
Cuts at: 4978 5010 5052 5084 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1752 1131 459 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3681 3927 669
Size: 988 281 1743 246 3079
Fragments arranged by size:
3079 1743 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6337
HpaI GTT'AAC
Cuts at: 3927 3927
Size: 6337
KpnI G_GTAC'C
Cuts at: 2554 3662 2554
Size: 1108 5229
MfeI C'AATT_G
Cuts at: 3936 3936
Size: 6337
MluI A'CGCG_T
Cuts at: 6213 6213
Size: 6337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5451 5635 1305
Size: 4146 184 2007
Fragments arranged by size:
4146 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2123 2540 3395 3449 10
Size: 54 1425 343 291 417 855 54 2898
Fragments arranged by size:
2898 1425 855 417 343 291 54 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 4295 4654 4813 518
Size: 648 849 2280 359 159 2042
Fragments arranged by size:
2280 2042 849 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2683 2773 3054 3133 3753 4430 5578 5823
Size: 1077 90 281 79 620 677 1148 245
Cuts at: 5823 1606
Size: 2120
Fragments arranged by size:
2120 1148 1077 677 620 281 245 90
79
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5785
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6337
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6337
NotI GC'GGCC_GC
Cuts at: 3784 3784
Size: 6337
NspI r_CATG'y
Cuts at: 1132 1229 2608 3695 5240 1132
Size: 97 1379 1087 1545 2229
Fragments arranged by size:
2229 1545 1379 1087 97
PasI CC'CwG_GG
Cuts at: 1348 2488 3296 3535 1348
Size: 1140 808 239 4150
Fragments arranged by size:
4150 1140 808 239
PciI A'CATG_T
Cuts at: 2604 5236 2604
Size: 2632 3705
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2547 2683 1108
Size: 1439 136 4762
Fragments arranged by size:
4762 1439 136
PfoI T'CCnGG_A
Cuts at: 2178 2196 2436 2694 3033 2178
Size: 18 240 258 339 5482
Fragments arranged by size:
5482 339 258 240 18
PmeI GTTT'AAAC
Cuts at: 3641 3641
Size: 6337
PmlI CAC'GTG
Cuts at: 2344 2344
Size: 6337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4406 4438 5919 5951 4406
Size: 32 1481 32 4792
Fragments arranged by size:
4792 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 2778 3158 3463 2725
Size: 53 380 305 5599
Fragments arranged by size:
5599 380 305 53
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6337
PsiI TTA'TAA
Cuts at: 3907 3907
Size: 6337
PspOMI G'GGCC_C
Cuts at: 3073 3073
Size: 6337
PstI C_TGCA'G
Cuts at: 830 2334 2904 3689 830
Size: 1504 570 785 3478
Fragments arranged by size:
3478 1504 785 570
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6337
PvuII CAG'CTG
Cuts at: 2683 2773 3133 2683
Size: 90 360 5887
Fragments arranged by size:
5887 360 90
SalI G'TCGA_C
Cuts at: 1936 3679 1936
Size: 1743 4594
SanDI GG'GwC_CC
Cuts at: 2778 3158 2778
Size: 380 5957
SbfI CC_TGCA'GG
Cuts at: 3689 3689
Size: 6337
ScaI AGT'ACT
Cuts at: 4585 6297 4585
Size: 1712 4625
SexAI A'CCwGG_T
Cuts at: 2271 2271
Size: 6337
SfcI C'TryA_G
Cuts at: 826 1045 2330 2900 3685 4820 5501 5692 826
Size: 219 1285 570 785 1135 681 191 1471
Fragments arranged by size:
1471 1285 1135 785 681 570 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3401 3401
Size: 6337
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6337
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6337
SmaI CCC'GGG
Cuts at: 1872 2972 3664 1872
Size: 1100 692 4545
Fragments arranged by size:
4545 1100 692
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3061 4441 5342 5604 820
Size: 197 926 1118 1380 901 262 1553
Fragments arranged by size:
1553 1380 1118 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4128 493
Size: 3635 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6337
SphI G_CATG'C
Cuts at: 1132 3695 1132
Size: 2563 3774
SspI AAT'ATT
Cuts at: 5 52 4261 5
Size: 47 4209 2081
Fragments arranged by size:
4209 2081 47
StuI AGG'CCT
Cuts at: 1517 2834 1517
Size: 1317 5020
StyI C'CwwG_G
Cuts at: 513 1065 2593 2835 3274 3776 513
Size: 552 1528 242 439 502 3074
Fragments arranged by size:
3074 1528 552 502 439 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4364 4381 4534 4719 5164 325
Size: 1255 2784 17 153 185 445 1498
Fragments arranged by size:
2784 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4583 6295 96
Size: 274 80 33 51 1443 2606 1712 138
Fragments arranged by size:
2606 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2095 2127 2333 2365 6297 6329 2095
Size: 32 206 32 3932 32 2103
Fragments arranged by size:
3932 2103 206 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6337
XbaI T'CTAG_A
Cuts at: 3673 3673
Size: 6337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2434 2434
Size: 6337
XhoI C'TCGA_G
Cuts at: 1943 3061 1943
Size: 1118 5219
XmaI C'CCGG_G
Cuts at: 1870 2970 3662 1870
Size: 1100 692 4545
Fragments arranged by size:
4545 1100 692
XmnI GAAnn'nnTTC
Cuts at: 4466 4466
Size: 6337
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2338 4145 276
Size: 53 83 186 936 804 1807 2468
Fragments arranged by size:
2468 1807 936 804 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BfrBI BsiWI BsmBI BstZ17I
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacI SacII
SapI SfoI SrfI SwaI