Restriction map for
pFN21AE0974
(Circular) (Six-base) MAPSORT of: pFN21AE0974.seq Check: 1523 from: 1 to: 6286
pFN21AE0974 6286 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2030 2082 2030
Size: 52 6234
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2200 2353 3629 1937
Size: 263 153 1276 4594
Fragments arranged by size:
4594 1276 263 153
Acc65I G'GTAC_C
Cuts at: 3607 3607
Size: 6286
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2268 2435 2754 2924 4349 5733 6001 1632
Size: 636 167 319 170 1425 1384 268 1917
Fragments arranged by size:
1917 1425 1384 636 319 268 170 167
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 2027 3403 5185 6162 1203
Size: 824 1376 1782 977 1327
Fragments arranged by size:
1782 1376 1327 977 824
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 5015 5015
Size: 6286
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6286
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3055 3087 3055
Size: 32 6254
AlwNI CAG_nnn'CTG
Cuts at: 2349 2365 2761 4029 5601 2349
Size: 16 396 1268 1572 3034
Fragments arranged by size:
3034 1572 1268 396 16
ApaI G_GGCC'C
Cuts at: 3568 3568
Size: 6286
ApaLI G'TGCA_C
Cuts at: 3080 4343 5499 3080
Size: 1263 1156 3867
Fragments arranged by size:
3867 1263 1156
ApoI r'AATT_y
Cuts at: 1493 3595 3821 3973 6082 1493
Size: 2102 226 152 2109 1697
Fragments arranged by size:
2109 2102 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4840 160
Size: 872 3808 1606
Fragments arranged by size:
3808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 2160 3507 3611 1870
Size: 73 217 1347 104 4545
Fragments arranged by size:
4545 1347 217 104 73
BamHI G'GATC_C
Cuts at: 3616 3983 3616
Size: 367 5919
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2453 2531 2909 3516 3607
Size: 325 217 627 666 78 378 607 91
Cuts at: 3607 5062 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 666 627 607 378 325 217
91 78
BanII G_rGCy'C
Cuts at: 1391 2966 3140 3568 3605 1391
Size: 1575 174 428 37 4072
Fragments arranged by size:
4072 1575 428 174 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2310 928
Size: 971 411 4904
Fragments arranged by size:
4904 971 411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4477 4511 1903
Size: 34 2540 34 3678
Fragments arranged by size:
3678 2540 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 2748 1548
Size: 1200 5086
BfrBI ATG'CAT
Cuts at: 2851 2851
Size: 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3002 4897 136
Size: 107 122 71 631 605 1330 1895 1525
Fragments arranged by size:
1895 1525 1330 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6281 6281
Size: 6286
BlpI GC'TnA_GC
Cuts at: 3703 3703
Size: 6286
Bme1580I G_kGCm'C
Cuts at: 1790 3084 3568 4347 5503 1790
Size: 1294 484 779 1156 2573
Fragments arranged by size:
2573 1294 1156 779 484
BmgBI CAC'GTC
Cuts at: 1318 3406 1318
Size: 2088 4198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3451 4017 4975
Size: 416 53 83 399 24 2034 566 958
Cuts at: 4975 442
Size: 1753
Fragments arranged by size:
2034 1753 958 566 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 2709 2920 4946 1239
Size: 1148 322 211 2026 2579
Fragments arranged by size:
2579 2026 1148 322 211
Bpu10I CC'TnA_GC
Cuts at: 3559 3559
Size: 6286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2581 2789 4411 5276 5574 2581
Size: 208 1622 865 298 3293
Fragments arranged by size:
3293 1622 865 298 208
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3497 4949 882
Size: 617 1998 1452 2219
Fragments arranged by size:
2219 1998 1452 617
BsaAI yAC'GTr
Cuts at: 493 1204 3412 4077 493
Size: 711 2208 665 2702
Fragments arranged by size:
2702 2208 711 665
BsaBI GATnn'nnATC
Cuts at: 2655 6280 2655
Size: 3625 2661
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4093 4475 5110 275
Size: 53 83 186 936 2560 382 635 1451
Fragments arranged by size:
2560 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4719 5117 5391 5538 5949 4719
Size: 398 274 147 411 5056
Fragments arranged by size:
5056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2713 2743 1178
Size: 30 165 30 1310 30 4721
Fragments arranged by size:
4721 1310 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 1186
Size: 886 5400
BseYI C'CCAG_C
Cuts at: 2773 5489 5985 2773
Size: 2716 496 3074
Fragments arranged by size:
3074 2716 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2008 1163
Size: 573 272 5441
Fragments arranged by size:
5441 573 272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2966 3084 4347 4432 5503 2966
Size: 118 1263 85 1071 3749
Fragments arranged by size:
3749 1263 1071 118 85
BsmI GAATG_Cn'
Cuts at: 3797 3890 3797
Size: 93 6193
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2966 3084 3140 3568 3605 4347 4432
Size: 399 1176 118 56 428 37 742 85
Cuts at: 4432 5503 1391
Size: 1071 2174
Fragments arranged by size:
2174 1176 1071 742 428 399 118 85
56 37
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 4175 5905 4175
Size: 1730 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2030 2082 2436 3574 3641 844
Size: 1186 52 354 1138 67 3489
Fragments arranged by size:
3489 1186 1138 354 67 52
BsrBI CCG'CTC
Cuts at: 1394 1823 4173 1394
Size: 429 2350 3507
Fragments arranged by size:
3507 2350 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4781 4955 65
Size: 1183 3533 174 1396
Fragments arranged by size:
3533 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2285 2996 4930 5117 1700
Size: 110 475 711 1934 187 2869
Fragments arranged by size:
2869 1934 711 475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 2267 3314 4346 5358 2267
Size: 1047 1032 1012 3195
Fragments arranged by size:
3195 1047 1032 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6286
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 3308 1184
Size: 2124 4162
BstXI CCAn_nnnn'nTGG
Cuts at: 3416 3416
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 2569 3616 3983 4368 4385 5837 5923 5935
Size: 597 1047 367 385 17 1452 86 12
Cuts at: 5935 6281 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1047 597 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2354 2354
Size: 6286
Bsu36I CC'TnA_GG
Cuts at: 2553 3297 2553
Size: 744 5542
BtgI C'CryG_G
Cuts at: 513 1065 2004 2813 2832 513
Size: 552 939 809 19 3967
Fragments arranged by size:
3967 939 809 552 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3421 487
Size: 683 56 2195 3352
Fragments arranged by size:
3352 2195 683 56
BtsI GCAGTG_nn'
Cuts at: 3553 3802 4596 4616 3553
Size: 249 794 20 5223
Fragments arranged by size:
5223 794 249 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2313 3003
Size: 54 1425 50 275 18 177 306 690
Cuts at: 3003 3733 4622 6251 8
Size: 730 889 1629 43
Fragments arranged by size:
1629 1425 889 730 690 306 275 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2057 3495 4216 1353
Size: 704 1438 721 3423
Fragments arranged by size:
3423 1438 721 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2956 3437 4873 5247 5393 387
Size: 1064 138 1367 481 1436 374 146 1280
Fragments arranged by size:
1436 1367 1280 1064 481 374 146 138
EcoICRI GAG'CTC
Cuts at: 2964 2964
Size: 6286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2268 2387 2435 2709 2754 2920 2924
Size: 393 636 119 48 274 45 166 4
Cuts at: 2924 4349 4946 5733 6001 1239
Size: 1425 597 787 268 1524
Fragments arranged by size:
1524 1425 787 636 597 393 274 268
166 119 48 45 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3317 3317
Size: 6286
EcoO109I rG'GnC_Cy
Cuts at: 2410 3175 3301 3564 3565 2410
Size: 765 126 263 1 5131
Fragments arranged by size:
5131 765 263 126 1
EcoRI G'AATT_C
Cuts at: 3595 3973 3595
Size: 378 5908
EcoRV GAT'ATC
Cuts at: 2593 2593
Size: 6286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3361 3361
Size: 6286
FspI TGC'GCA
Cuts at: 2879 4792 2879
Size: 1913 4373
HaeII r_GCGC'y
Cuts at: 1678 2994 5433 1678
Size: 1316 2439 2531
Fragments arranged by size:
2531 2439 1316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2554 2586 2690 2722 2839
Size: 32 135 32 982 32 104 32 117
Cuts at: 2839 2871 3256 3288 4927 4959 5001 5033 1373
Size: 32 385 32 1639 32 42 32 2626
Fragments arranged by size:
2626 1639 982 385 135 117 104 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2201 2377 2825 3630 3876 669
Size: 988 281 263 176 448 805 246 3079
Fragments arranged by size:
3079 988 805 448 281 263 246 176
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6286
HpaI GTT'AAC
Cuts at: 2377 3876 2377
Size: 1499 4787
KpnI G_GTAC'C
Cuts at: 3611 3611
Size: 6286
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3021 5400 5584 1305
Size: 1716 2379 184 2007
Fragments arranged by size:
2379 2007 1716 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2315 10
Size: 54 1425 343 177 306 3981
Fragments arranged by size:
3981 1425 343 306 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2391 2713 2924 4244 4603 4762 518
Size: 648 1225 322 211 1320 359 159 2042
Fragments arranged by size:
2042 1320 1225 648 359 322 211 159
MspA1I CmG'CkG
Cuts at: 1606 3220 3580 3702 4379 5527 5772 1606
Size: 1614 360 122 677 1148 245 2120
Fragments arranged by size:
2120 1614 1148 677 360 245 122
NaeI GCC'GGC
Cuts at: 2998 2998
Size: 6286
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4795
Fragments arranged by size:
4795 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NgoMIV G'CCGG_C
Cuts at: 2996 2996
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NsiI A_TGCA'T
Cuts at: 2853 2853
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 3644 5189 1132
Size: 97 2415 1545 2229
Fragments arranged by size:
2415 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 5185 5185
Size: 6286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6286
PfoI T'CCnGG_A
Cuts at: 2936 2936
Size: 6286
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PmlI CAC'GTG
Cuts at: 3412 3412
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 5868 5900 4355
Size: 32 1481 32 4741
Fragments arranged by size:
4741 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2410 3175 2410
Size: 765 5521
PshAI GACnn'nnGTC
Cuts at: 1613 3509 1613
Size: 1896 4390
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PspOMI G'GGCC_C
Cuts at: 3564 3564
Size: 6286
PstI C_TGCA'G
Cuts at: 830 3585 3638 830
Size: 2755 53 3478
Fragments arranged by size:
3478 2755 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
PvuII CAG'CTG
Cuts at: 3220 3220
Size: 6286
SacI G_AGCT'C
Cuts at: 2966 2966
Size: 6286
SalI G'TCGA_C
Cuts at: 1936 2199 3628 1936
Size: 263 1429 4594
Fragments arranged by size:
4594 1429 263
SapI GCTCTTCn'nnn_
Cuts at: 2057 2057
Size: 6286
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 2324 4534 6246 2324
Size: 2210 1712 2364
Fragments arranged by size:
2364 2210 1712
SfcI C'TryA_G
Cuts at: 826 1045 2326 3120 3581 3634 4769 5450 5641
Size: 219 1281 794 461 53 1135 681 191
Cuts at: 5641 826
Size: 1471
Fragments arranged by size:
1471 1281 1135 794 681 461 219 191
53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 2162 3509 3613 1872
Size: 290 1347 104 4545
Fragments arranged by size:
4545 1347 290 104
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2560 2804 4390 5291 5553 820
Size: 197 926 617 244 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 617 262 244 197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 3644 1132
Size: 2512 3774
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 3254 3279 1517
Size: 1737 25 4524
Fragments arranged by size:
4524 1737 25
StyI C'CwwG_G
Cuts at: 513 1065 2004 3304 3725 513
Size: 552 939 1300 421 3074
Fragments arranged by size:
3074 1300 939 552 421
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3470 4313 4330 4483 4668 5113 325
Size: 1255 1890 843 17 153 185 445 1498
Fragments arranged by size:
1890 1498 1255 843 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 2494 3459
Size: 274 80 33 51 1443 345 172 965
Cuts at: 3459 4532 6244 96
Size: 1073 1712 138
Fragments arranged by size:
1712 1443 1073 965 345 274 172 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2571 2603 2713 2745 3416 3448 3518 3550 6246
Size: 32 110 32 671 32 70 32 2696
Cuts at: 6246 6278 2571
Size: 32 2579
Fragments arranged by size:
2696 2579 671 110 70 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2399 1316
Size: 1083 5203
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2712 2712
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 2160 3507 3611 1870
Size: 290 1347 104 4545
Fragments arranged by size:
4545 1347 290 104
XmnI GAAnn'nnTTC
Cuts at: 2256 4415 2256
Size: 2159 4127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BplI BsiWI
BsmBI DraIII FseI FspAI KasI NarI NruI PacI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI