Restriction map for
pFN21AE0973
(Circular) (Six-base) MAPSORT of: pFN21AE0973.seq Check: 8179 from: 1 to: 6340
pFN21AE0973 6340 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4150 278
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3683 1937
Size: 1746 4594
Acc65I G'GTAC_C
Cuts at: 3661 3661
Size: 6340
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2748 2942 4403 5787 6055 1632
Size: 1116 194 1461 1384 268 1917
Fragments arranged by size:
1917 1461 1384 1116 268 194
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 2187 2266 2769 5239 6216 1203
Size: 984 79 503 2470 977 1327
Fragments arranged by size:
2470 1327 984 977 503 79
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 5069 5069
Size: 6340
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2263 1184
Size: 1079 5261
AlwNI CAG_nnn'CTG
Cuts at: 4083 5655 4083
Size: 1572 4768
ApaI G_GGCC'C
Cuts at: 3314 3314
Size: 6340
ApaLI G'TGCA_C
Cuts at: 2030 3296 4397 5553 2030
Size: 1266 1101 1156 2817
Fragments arranged by size:
2817 1266 1156 1101
ApoI r'AATT_y
Cuts at: 1493 2331 3008 3649 3875 4027 6136 1493
Size: 838 677 641 226 152 2109 1697
Fragments arranged by size:
2109 1697 838 677 641 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4894 160
Size: 872 3862 1606
Fragments arranged by size:
3862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 3665 1870
Size: 73 1722 4545
Fragments arranged by size:
4545 1722 73
BamHI G'GATC_C
Cuts at: 3670 4037 3670
Size: 367 5973
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2705 3661 5116 618
Size: 325 217 627 918 956 1455 1842
Fragments arranged by size:
1842 1455 956 918 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3314 3462 3659 1391
Size: 1923 148 197 4072
Fragments arranged by size:
4072 1923 197 148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2948 3144 3336 928
Size: 971 1049 196 192 3932
Fragments arranged by size:
3932 1049 971 196 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4531 4565 1903
Size: 34 2594 34 3678
Fragments arranged by size:
3678 2594 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4232 5442 4232
Size: 1210 5130
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6340
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 2153 4951
Size: 107 122 71 631 605 418 63 2798
Cuts at: 4951 136
Size: 1525
Fragments arranged by size:
2798 1525 631 605 418 122 107 71
63
BglII A'GATC_T
Cuts at: 6335 6335
Size: 6340
BlpI GC'TnA_GC
Cuts at: 3757 3757
Size: 6340
Bme1580I G_kGCm'C
Cuts at: 1790 2034 3300 3314 4401 5557 1790
Size: 244 1266 14 1087 1156 2573
Fragments arranged by size:
2573 1266 1156 1087 244 14
BmgBI CAC'GTC
Cuts at: 1318 2035 1318
Size: 717 5623
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3157 4071 5029
Size: 416 53 83 399 24 1740 914 958
Cuts at: 5029 442
Size: 1753
Fragments arranged by size:
1753 1740 958 914 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6340
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3481 3481
Size: 6340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3370 3408 5000 1239
Size: 2131 38 1592 2579
Fragments arranged by size:
2579 2131 1592 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4465 5330 5628 4465
Size: 865 298 5177
Fragments arranged by size:
5177 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5003 882
Size: 617 3504 2219
Fragments arranged by size:
3504 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4131 493
Size: 711 2927 2702
Fragments arranged by size:
2927 2702 711
BsaBI GATnn'nnATC
Cuts at: 6334 6334
Size: 6340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4147 4529 5164 275
Size: 53 83 186 936 2614 382 635 1451
Fragments arranged by size:
2614 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4773 5171 5445 5592 6003 4773
Size: 398 274 147 411 5110
Fragments arranged by size:
5110 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6115
Fragments arranged by size:
6115 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2487 1186
Size: 1301 5039
BseYI C'CCAG_C
Cuts at: 2046 5543 6039 2046
Size: 3497 496 2347
Fragments arranged by size:
3497 2347 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2371 1163
Size: 573 635 5132
Fragments arranged by size:
5132 635 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 3790 4551 5579 1540
Size: 275 186 93 1696 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 275 186 93
BsiHKAI G_wGCw'C
Cuts at: 2034 3300 3462 4401 4486 5557 2034
Size: 1266 162 939 85 1071 2817
Fragments arranged by size:
2817 1266 1071 939 162 85
BsiWI C'GTAC_G
Cuts at: 2190 2190
Size: 6340
BsmI GAATG_Cn'
Cuts at: 3851 3944 3851
Size: 93 6247
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 3300 3314 3462 3659 4401 4486
Size: 399 244 1266 14 148 197 742 85
Cuts at: 4486 5557 1391
Size: 1071 2174
Fragments arranged by size:
2174 1266 1071 742 399 244 197 148
85 14
BspEI T'CCGG_A
Cuts at: 6003 6003
Size: 6340
BspHI T'CATG_A
Cuts at: 2415 4229 5959 2415
Size: 1814 1730 2796
Fragments arranged by size:
2796 1814 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3695 844
Size: 2851 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 4227 1394
Size: 429 196 2208 3507
Fragments arranged by size:
3507 2208 429 196
BsrDI GCAATG_nn'
Cuts at: 65 1248 4835 5009 65
Size: 1183 3587 174 1396
Fragments arranged by size:
3587 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 4984 5171 1700
Size: 110 297 2877 187 2869
Fragments arranged by size:
2877 2869 297 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6340
BssSI C'ACGA_G
Cuts at: 4400 5412 4400
Size: 1012 5328
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3257 794
Size: 2463 3877
BstBI TT'CG_AA
Cuts at: 5144 5144
Size: 6340
BstEII G'GTnAC_C
Cuts at: 1184 3119 1184
Size: 1935 4405
BstYI r'GATC_y
Cuts at: 1972 2965 3670 4037 4422 4439 5891 5977 5989
Size: 993 705 367 385 17 1452 86 12
Cuts at: 5989 6335 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 993 705 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 513
Size: 552 939 120 4729
Fragments arranged by size:
4729 939 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5601
Fragments arranged by size:
5601 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 3258 3856 4650 4670 2221
Size: 1037 598 794 20 3891
Fragments arranged by size:
3891 1037 794 598 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2862 2897 529
Size: 35 2298 35 3972
Fragments arranged by size:
3972 2298 35 35
DraI TTT'AAA
Cuts at: 3000 3546 3644 3000
Size: 546 98 5696
Fragments arranged by size:
5696 546 98
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5347 809
Size: 507 4031 1802
Fragments arranged by size:
4031 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 3787 4676
Size: 54 1425 50 275 18 261 1696 889
Cuts at: 4676 6305 8
Size: 1629 43
Fragments arranged by size:
1696 1629 1425 889 275 261 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2091 3787 1537
Size: 275 279 1696 4090
Fragments arranged by size:
4090 1696 279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2493 3094 3173 3217 3424 3467 4270 1353
Size: 1140 601 79 44 207 43 803 3423
Fragments arranged by size:
3423 1140 803 601 207 79 44 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4927 5301 5447 387
Size: 1064 138 435 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 3460 3460
Size: 6340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2748 2942 3370 3408 4403 5000 5787
Size: 393 1116 194 428 38 995 597 787
Cuts at: 5787 6055 1239
Size: 268 1524
Fragments arranged by size:
1524 1116 995 787 597 428 393 268
194 38
EcoO109I rG'GnC_Cy
Cuts at: 3310 3310
Size: 6340
EcoRI G'AATT_C
Cuts at: 3649 4027 3649
Size: 378 5962
EcoRV GAT'ATC
Cuts at: 2449 2449
Size: 6340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2420 2420
Size: 6340
FspI TGC'GCA
Cuts at: 4846 4846
Size: 6340
HaeII r_GCGC'y
Cuts at: 1678 2108 2819 5487 1678
Size: 430 711 2668 2531
Fragments arranged by size:
2668 2531 711 430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3434 3466 4981 5013 5055
Size: 32 135 32 1862 32 1515 32 42
Cuts at: 5055 5087 1373
Size: 32 2626
Fragments arranged by size:
2626 1862 1515 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2467 2856 3684 3930 669
Size: 988 281 529 389 828 246 3079
Fragments arranged by size:
3079 988 828 529 389 281 246
HindIII A'AGCT_T
Cuts at: 748 3342 3540 748
Size: 2594 198 3548
Fragments arranged by size:
3548 2594 198
HpaI GTT'AAC
Cuts at: 3930 3930
Size: 6340
KpnI G_GTAC'C
Cuts at: 3665 3665
Size: 6340
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 2187 6216 2187
Size: 4029 2311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2424 2696 3567 5454 5638 1305
Size: 1119 272 871 1887 184 2007
Fragments arranged by size:
2007 1887 1119 871 272 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4518
Fragments arranged by size:
4518 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2484 2843 4298 4657 4816 518
Size: 648 1318 359 1455 359 159 2042
Fragments arranged by size:
2042 1455 1318 648 359 359 159
MspA1I CmG'CkG
Cuts at: 1606 2069 2126 3756 4433 5581 5826 1606
Size: 463 57 1630 677 1148 245 2120
Fragments arranged by size:
2120 1630 1148 677 463 245 57
NaeI GCC'GGC
Cuts at: 2109 2109
Size: 6340
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4849
Fragments arranged by size:
4849 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6340
NgoMIV G'CCGG_C
Cuts at: 2107 2107
Size: 6340
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6340
NotI GC'GGCC_GC
Cuts at: 2091 3787 2091
Size: 1696 4644
NspI r_CATG'y
Cuts at: 1132 1229 2773 3698 5243 1132
Size: 97 1544 925 1545 2229
Fragments arranged by size:
2229 1545 1544 925 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6340
PciI A'CATG_T
Cuts at: 2769 5239 2769
Size: 2470 3870
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6340
PfoI T'CCnGG_A
Cuts at: 3424 3424
Size: 6340
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4409 4441 5922 5954 4409
Size: 32 1481 32 4795
Fragments arranged by size:
4795 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6340
PsiI TTA'TAA
Cuts at: 3910 3910
Size: 6340
PspOMI G'GGCC_C
Cuts at: 3310 3310
Size: 6340
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3020 3052 3087 3119 3020
Size: 32 35 32 6241
Fragments arranged by size:
6241 35 32 32
PstI C_TGCA'G
Cuts at: 830 2183 2202 3692 830
Size: 1353 19 1490 3478
Fragments arranged by size:
3478 1490 1353 19
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
SacI G_AGCT'C
Cuts at: 3462 3462
Size: 6340
SacII CC_GC'GG
Cuts at: 2127 2127
Size: 6340
SalI G'TCGA_C
Cuts at: 1936 3682 1936
Size: 1746 4594
SapI GCTCTTCn'nnn_
Cuts at: 3173 3467 3173
Size: 294 6046
SbfI CC_TGCA'GG
Cuts at: 3692 3692
Size: 6340
ScaI AGT'ACT
Cuts at: 4588 6300 4588
Size: 1712 4628
SfcI C'TryA_G
Cuts at: 826 1045 2179 2198 3334 3688 4823 5504 5695
Size: 219 1134 19 1136 354 1135 681 191
Cuts at: 5695 826
Size: 1471
Fragments arranged by size:
1471 1136 1135 1134 681 354 219 191
19
SfiI GGCCn_nnn'nGGCC
Cuts at: 2090 2090
Size: 6340
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 3667 1872
Size: 1795 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4444 5345 5607 820
Size: 197 926 2501 901 262 1553
Fragments arranged by size:
2501 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4131 493
Size: 3638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6340
SphI G_CATG'C
Cuts at: 1132 3698 1132
Size: 2566 3774
SspI AAT'ATT
Cuts at: 5 52 2596 4264 5
Size: 47 2544 1668 2081
Fragments arranged by size:
2544 2081 1668 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6340
StyI C'CwwG_G
Cuts at: 513 1065 2004 3779 513
Size: 552 939 1775 3074
Fragments arranged by size:
3074 1775 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2594 2890 3331 4367 4384 4537 4722
Size: 1255 1014 296 441 1036 17 153 185
Cuts at: 4722 5167 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1036 1014 445 441 296 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2620 4586 6298
Size: 274 80 33 51 1443 643 1966 1712
Cuts at: 6298 96
Size: 138
Fragments arranged by size:
1966 1712 1443 643 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3137 3169 6300 6332 3137
Size: 32 3131 32 3145
Fragments arranged by size:
3145 3131 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6340
XbaI T'CTAG_A
Cuts at: 3676 3676
Size: 6340
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6340
XmaI C'CCGG_G
Cuts at: 1870 3665 1870
Size: 1795 4545
XmnI GAAnn'nnTTC
Cuts at: 4469 4469
Size: 6340
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4148 276
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspXI PvuII RsrII SanDI SexAI SfoI SrfI
SwaI XcmI