Restriction map for
pFN21AE0970
(Circular) (Six-base) MAPSORT of: pFN21AE0970.seq Check: 2467 from: 1 to: 6274
pFN21AE0970 6274 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2856 4084 278
Size: 53 83 186 936 1320 1228 2468
Fragments arranged by size:
2468 1320 1228 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3617 1937
Size: 1680 4594
Acc65I G'GTAC_C
Cuts at: 3595 3595
Size: 6274
AclI AA'CG_TT
Cuts at: 1563 4401 4774 1563
Size: 2838 373 3063
Fragments arranged by size:
3063 2838 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 2369 3418 4337 5721 5989 1632
Size: 563 174 1049 919 1384 268 1917
Fragments arranged by size:
1917 1384 1049 919 563 268 174
AfeI AGC'GCT
Cuts at: 3396 3396
Size: 6274
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6077
AflIII A'CryG_T
Cuts at: 1203 3372 5173 6150 1203
Size: 2169 1801 977 1327
Fragments arranged by size:
2169 1801 1327 977
AgeI A'CCGG_T
Cuts at: 5105 5105
Size: 6274
AhdI GACnn_n'nnGTC
Cuts at: 5003 5003
Size: 6274
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6274
AlwNI CAG_nnn'CTG
Cuts at: 2302 3061 3106 3289 4017 5589 2302
Size: 759 45 183 728 1572 2987
Fragments arranged by size:
2987 1572 759 728 183 45
ApaLI G'TGCA_C
Cuts at: 2225 2918 4331 5487 2225
Size: 693 1413 1156 3012
Fragments arranged by size:
3012 1413 1156 693
ApoI r'AATT_y
Cuts at: 1493 3026 3583 3809 3961 6070 1493
Size: 1533 557 226 152 2109 1697
Fragments arranged by size:
2109 1697 1533 557 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4828 160
Size: 872 3796 1606
Fragments arranged by size:
3796 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
AvaI C'yCGr_G
Cuts at: 1870 1943 3599 1870
Size: 73 1656 4545
Fragments arranged by size:
4545 1656 73
BamHI G'GATC_C
Cuts at: 3604 3971 3604
Size: 367 5907
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2276 3341 3595 5050 618
Size: 325 217 627 489 1065 254 1455 1842
Fragments arranged by size:
1842 1455 1065 627 489 325 254 217
BanII G_rGCy'C
Cuts at: 1391 2259 2311 2557 2564 3498 3593 1391
Size: 868 52 246 7 934 95 4072
Fragments arranged by size:
4072 934 868 246 95 52 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3308 928
Size: 971 1409 3894
Fragments arranged by size:
3894 1409 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4465 4499 1903
Size: 34 2528 34 3678
Fragments arranged by size:
3678 2528 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4166 5376 4166
Size: 1210 5064
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6274
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2480 2877
Size: 107 122 71 631 605 343 465 397
Cuts at: 2877 4885 136
Size: 2008 1525
Fragments arranged by size:
2008 1525 631 605 465 397 343 122
107 71
BglII A'GATC_T
Cuts at: 2216 2413 2862 3447 6269 2216
Size: 197 449 585 2822 2221
Fragments arranged by size:
2822 2221 585 449 197
BlpI GC'TnA_GC
Cuts at: 3402 3691 3402
Size: 289 5985
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2279 2922 3344 4335 5491 1790
Size: 439 50 643 422 991 1156 2573
Fragments arranged by size:
2573 1156 991 643 439 422 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6274
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2770 3116 4005
Size: 416 53 83 399 24 1353 346 889
Cuts at: 4005 4963 442
Size: 958 1753
Fragments arranged by size:
1753 1353 958 889 416 399 346 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3401 1056
Size: 2345 3929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4934 1239
Size: 3695 2579
Bpu10I CC'TnA_GC
Cuts at: 2723 2723
Size: 6274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 4399 5264 5562 2006
Size: 2393 865 298 2718
Fragments arranged by size:
2718 2393 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4937 882
Size: 617 3438 2219
Fragments arranged by size:
3438 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3274 4065 493
Size: 711 2070 791 2702
Fragments arranged by size:
2702 2070 791 711
BsaBI GATnn'nnATC
Cuts at: 6268 6268
Size: 6274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2245 2853 4081 4463
Size: 53 83 186 936 712 608 1228 382
Cuts at: 4463 5098 275
Size: 635 1451
Fragments arranged by size:
1451 1228 936 712 635 608 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4707 5105 5379 5526 5937 4707
Size: 398 274 147 411 5044
Fragments arranged by size:
5044 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6049
Fragments arranged by size:
6049 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2345 3254 1186
Size: 1027 132 909 4206
Fragments arranged by size:
4206 1027 909 132
BseYI C'CCAG_C
Cuts at: 5477 5973 5477
Size: 496 5778
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 1163
Size: 573 606 5095
Fragments arranged by size:
5095 606 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3158 3724 4485 5513 1540
Size: 275 186 1157 566 761 1028 2301
Fragments arranged by size:
2301 1157 1028 761 566 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 2259 2564 2592 2922 3408 3498 4335 4420
Size: 30 305 28 330 486 90 837 85
Cuts at: 4420 5491 2229
Size: 1071 3012
Fragments arranged by size:
3012 1071 837 486 330 305 90 85
30 28
BsmI GAATG_Cn'
Cuts at: 2704 3785 3878 2704
Size: 1081 93 5100
Fragments arranged by size:
5100 1081 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2252 2252
Size: 6274
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2259 2279 2311 2557 2564 2592
Size: 399 439 30 20 32 246 7 28
Cuts at: 2592 2922 3344 3408 3498 3593 4335 4420 5491
Size: 330 422 64 90 95 742 85 1071
Cuts at: 5491 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 439 422 399 330 246
95 90 85 64 32 30 28 20
7
BspEI T'CCGG_A
Cuts at: 5937 5937
Size: 6274
BspHI T'CATG_A
Cuts at: 4163 5893 4163
Size: 1730 4544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2670 2803 3321 3629 844
Size: 1826 133 518 308 3489
Fragments arranged by size:
3489 1826 518 308 133
BsrBI CCG'CTC
Cuts at: 1394 1823 2779 4161 1394
Size: 429 956 1382 3507
Fragments arranged by size:
3507 1382 956 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2947 4769 4943 65
Size: 1183 1699 1822 174 1396
Fragments arranged by size:
1822 1699 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4918 5105 1700
Size: 110 3108 187 2869
Fragments arranged by size:
3108 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6274
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6274
BssSI C'ACGA_G
Cuts at: 4334 5346 4334
Size: 1012 5262
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6274
BstBI TT'CG_AA
Cuts at: 5078 5078
Size: 6274
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6274
BstYI r'GATC_y
Cuts at: 1972 2216 2391 2413 2427 2862 3118 3447 3604
Size: 244 175 22 14 435 256 329 157
Cuts at: 3604 3971 4356 4373 5825 5911 5923 6269 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 435 385 367 346 329 256
244 175 157 86 22 17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2772 2998 513
Size: 552 939 768 226 3789
Fragments arranged by size:
3789 939 768 552 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5535
Fragments arranged by size:
5535 683 56
BtsI GCAGTG_nn'
Cuts at: 2505 2920 3198 3790 4584 4604 2505
Size: 415 278 592 794 20 4175
Fragments arranged by size:
4175 794 592 415 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6239
DraI TTT'AAA
Cuts at: 3578 3578
Size: 6274
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5281 809
Size: 507 3965 1802
Fragments arranged by size:
3965 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2523 3234
Size: 54 1425 50 275 18 177 516 711
Cuts at: 3234 3721 4610 6239 8
Size: 487 889 1629 43
Fragments arranged by size:
1629 1425 889 711 516 487 275 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3721 1537
Size: 275 1909 4090
Fragments arranged by size:
4090 1909 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2171 3074 4204 1353
Size: 818 903 1130 3423
Fragments arranged by size:
3423 1130 903 818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2384 2530 2993 3020 4861 5235
Size: 1064 138 795 146 463 27 1841 374
Cuts at: 5235 5381 387
Size: 146 1280
Fragments arranged by size:
1841 1280 1064 795 463 374 146 146
138 27
EcoICRI GAG'CTC
Cuts at: 2257 2562 3496 2257
Size: 305 934 5035
Fragments arranged by size:
5035 934 305
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2369 3418 4337 4934 5721 5989
Size: 393 563 174 1049 919 597 787 268
Cuts at: 5989 1239
Size: 1524
Fragments arranged by size:
1524 1049 919 787 597 563 393 268
174
EcoNI CCTnn'n_nnAGG
Cuts at: 2483 2582 2483
Size: 99 6175
EcoO109I rG'GnC_Cy
Cuts at: 2746 2764 2782 3441 2746
Size: 18 18 659 5579
Fragments arranged by size:
5579 659 18 18
EcoRI G'AATT_C
Cuts at: 3026 3583 3961 3026
Size: 557 378 5339
Fragments arranged by size:
5339 557 378
FspI TGC'GCA
Cuts at: 4780 4780
Size: 6274
HaeII r_GCGC'y
Cuts at: 1678 2112 3357 3398 5421 1678
Size: 434 1245 41 2023 2531
Fragments arranged by size:
2531 2023 1245 434 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2696 2728 3244 3276 4915
Size: 32 135 32 1124 32 516 32 1639
Cuts at: 4915 4947 4989 5021 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1639 1124 516 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3172 3618 3864 669
Size: 988 281 1234 446 246 3079
Fragments arranged by size:
3079 1234 988 446 281 246
HindIII A'AGCT_T
Cuts at: 748 2024 748
Size: 1276 4998
HpaI GTT'AAC
Cuts at: 3864 3864
Size: 6274
KpnI G_GTAC'C
Cuts at: 3599 3599
Size: 6274
MfeI C'AATT_G
Cuts at: 3873 3873
Size: 6274
MluI A'CGCG_T
Cuts at: 6150 6150
Size: 6274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2973 5388 5572 1305
Size: 1668 2415 184 2007
Fragments arranged by size:
2415 2007 1668 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4275
Fragments arranged by size:
4275 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 4232 4591 4750 518
Size: 648 1122 1944 359 159 2042
Fragments arranged by size:
2042 1944 1122 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2212 2611 3690 4367 5515 5760 1606
Size: 606 399 1079 677 1148 245 2120
Fragments arranged by size:
2120 1148 1079 677 606 399 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2998 513
Size: 552 939 994 3789
Fragments arranged by size:
3789 994 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6274
NheI G'CTAG_C
Cuts at: 1052 3397 1052
Size: 2345 3929
NotI GC'GGCC_GC
Cuts at: 3721 3721
Size: 6274
NspI r_CATG'y
Cuts at: 1132 1229 2386 3145 3376 3632 5177 1132
Size: 97 1157 759 231 256 1545 2229
Fragments arranged by size:
2229 1545 1157 759 256 231 97
PasI CC'CwG_GG
Cuts at: 1348 3337 1348
Size: 1989 4285
PciI A'CATG_T
Cuts at: 3372 5173 3372
Size: 1801 4473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2302 3106 1108
Size: 1194 804 4276
Fragments arranged by size:
4276 1194 804
PmeI GTTT'AAAC
Cuts at: 3578 3578
Size: 6274
PmlI CAC'GTG
Cuts at: 3274 3274
Size: 6274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 5856 5888 4343
Size: 32 1481 32 4729
Fragments arranged by size:
4729 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2746 2764 2782 3441 2746
Size: 18 18 659 5579
Fragments arranged by size:
5579 659 18 18
PshAI GACnn'nnGTC
Cuts at: 1613 2144 1613
Size: 531 5743
PsiI TTA'TAA
Cuts at: 3844 3844
Size: 6274
PstI C_TGCA'G
Cuts at: 830 3626 830
Size: 2796 3478
PvuI CG_AT'CG
Cuts at: 2001 3158 2001
Size: 1157 5117
PvuII CAG'CTG
Cuts at: 2212 2611 2212
Size: 399 5875
SacI G_AGCT'C
Cuts at: 2259 2564 3498 2259
Size: 305 934 5035
Fragments arranged by size:
5035 934 305
SalI G'TCGA_C
Cuts at: 1936 3616 1936
Size: 1680 4594
SbfI CC_TGCA'GG
Cuts at: 3626 3626
Size: 6274
ScaI AGT'ACT
Cuts at: 2087 2204 4522 6234 2087
Size: 117 2318 1712 2127
Fragments arranged by size:
2318 2127 1712 117
SfcI C'TryA_G
Cuts at: 826 1045 2074 3622 4757 5438 5629 826
Size: 219 1029 1548 1135 681 191 1471
Fragments arranged by size:
1548 1471 1135 1029 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2480 2480
Size: 6274
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6274
SmaI CCC'GGG
Cuts at: 1872 3601 1872
Size: 1729 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 4378 5279 5541 820
Size: 197 926 78 2357 901 262 1553
Fragments arranged by size:
2357 1553 926 901 262 197 78
SnaBI TAC'GTA
Cuts at: 493 4065 493
Size: 3572 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6274
SphI G_CATG'C
Cuts at: 1132 2386 3145 3632 1132
Size: 1254 759 487 3774
Fragments arranged by size:
3774 1254 759 487
SspI AAT'ATT
Cuts at: 5 52 4198 5
Size: 47 4146 2081
Fragments arranged by size:
4146 2081 47
StuI AGG'CCT
Cuts at: 1517 3464 1517
Size: 1947 4327
StyI C'CwwG_G
Cuts at: 513 1065 2004 2998 3713 513
Size: 552 939 994 715 3074
Fragments arranged by size:
3074 994 939 715 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2556 3157 4301 4318 4471 4656 5101
Size: 1255 976 601 1144 17 153 185 445
Cuts at: 5101 325
Size: 1498
Fragments arranged by size:
1498 1255 1144 976 601 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2085 2202 2334
Size: 274 80 33 51 1443 108 117 132
Cuts at: 2334 2944 3093 4520 6232 96
Size: 610 149 1427 1712 138
Fragments arranged by size:
1712 1443 1427 610 274 149 138 132
117 108 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6234 6266 6234
Size: 32 6242
Tth111I GACn'n_nGTC
Cuts at: 1316 3167 3533 1316
Size: 1851 366 4057
Fragments arranged by size:
4057 1851 366
XbaI T'CTAG_A
Cuts at: 3610 3610
Size: 6274
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6274
XmaI C'CCGG_G
Cuts at: 1870 3599 1870
Size: 1729 4545
XmnI GAAnn'nnTTC
Cuts at: 4403 4403
Size: 6274
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2854 4082 276
Size: 53 83 186 936 1320 1228 2468
Fragments arranged by size:
2468 1320 1228 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfoI SrfI SwaI XcmI