Restriction map for
pFN21AE0969
(Circular) (Six-base) MAPSORT of: pFN21AE0969.seq Check: 3763 from: 1 to: 6286
pFN21AE0969 6286 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3004 3629 1937
Size: 1067 625 4594
Fragments arranged by size:
4594 1067 625
Acc65I G'GTAC_C
Cuts at: 2454 3607 2454
Size: 1153 5133
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2268 2946 3330 3436 3465 3546 4349 5733
Size: 636 678 384 106 29 81 803 1384
Cuts at: 5733 6001 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 803 678 636 384 268 106
81 29
AflII C'TTAA_G
Cuts at: 820 1017 2311 820
Size: 197 1294 4795
Fragments arranged by size:
4795 1294 197
AflIII A'CryG_T
Cuts at: 1203 5185 6162 1203
Size: 3982 977 1327
Fragments arranged by size:
3982 1327 977
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 5015 5015
Size: 6286
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6286
AlwNI CAG_nnn'CTG
Cuts at: 3510 4029 5601 3510
Size: 519 1572 4195
Fragments arranged by size:
4195 1572 519
ApaLI G'TGCA_C
Cuts at: 4343 5499 4343
Size: 1156 5130
ApoI r'AATT_y
Cuts at: 1493 3219 3418 3595 3821 3973 6082 1493
Size: 1726 199 177 226 152 2109 1697
Fragments arranged by size:
2109 1726 1697 226 199 177 152
AseI AT'TA_AT
Cuts at: 160 1032 4840 160
Size: 872 3808 1606
Fragments arranged by size:
3808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 3611 1870
Size: 73 1668 4545
Fragments arranged by size:
4545 1668 73
BamHI G'GATC_C
Cuts at: 2549 3616 3983 2549
Size: 1067 367 4852
Fragments arranged by size:
4852 1067 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2454 3475 3607 5062 618
Size: 325 217 627 667 1021 132 1455 1842
Fragments arranged by size:
1842 1455 1021 667 627 325 217 132
BanII G_rGCy'C
Cuts at: 1391 3605 1391
Size: 2214 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4477 4511 1903
Size: 34 2540 34 3678
Fragments arranged by size:
3678 2540 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6286
BfrBI ATG'CAT
Cuts at: 3210 3210
Size: 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4897 136
Size: 107 122 71 631 605 3225 1525
Fragments arranged by size:
3225 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6281 6281
Size: 6286
BlpI GC'TnA_GC
Cuts at: 2153 3703 2153
Size: 1550 4736
Bme1580I G_kGCm'C
Cuts at: 1790 4347 5503 1790
Size: 2557 1156 2573
Fragments arranged by size:
2573 2557 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4017 4975 442
Size: 416 53 83 399 24 2600 958 1753
Fragments arranged by size:
2600 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 3167 4946 1239
Size: 1121 807 1779 2579
Fragments arranged by size:
2579 1779 1121 807
Bpu10I CC'TnA_GC
Cuts at: 2099 3089 2099
Size: 990 5296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4411 5276 5574 4411
Size: 865 298 5123
Fragments arranged by size:
5123 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2367 2720 4949 882
Size: 617 868 353 2229 2219
Fragments arranged by size:
2229 2219 868 617 353
BsaAI yAC'GTr
Cuts at: 493 1204 4077 493
Size: 711 2873 2702
Fragments arranged by size:
2873 2702 711
BsaBI GATnn'nnATC
Cuts at: 6280 6280
Size: 6286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4093 4475 5110 275
Size: 53 83 186 936 2560 382 635 1451
Fragments arranged by size:
2560 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4719 5117 5391 5538 5949 4719
Size: 398 274 147 411 5056
Fragments arranged by size:
5056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6061
Fragments arranged by size:
6061 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6286
BseYI C'CCAG_C
Cuts at: 2133 2774 5489 5985 2133
Size: 641 2715 496 2434
Fragments arranged by size:
2715 2434 641 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 2216 1163
Size: 573 351 129 5233
Fragments arranged by size:
5233 573 351 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2105 4347 4432 5503 2105
Size: 2242 85 1071 2888
Fragments arranged by size:
2888 2242 1071 85
BsmI GAATG_Cn'
Cuts at: 3797 3890 3797
Size: 93 6193
BsmBI CGTCTCn'nnnn_
Cuts at: 2732 2732
Size: 6286
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 3605 4347 4432 5503 1391
Size: 399 315 1500 742 85 1071 2174
Fragments arranged by size:
2174 1500 1071 742 399 315 85
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 2718 4175 5905 2718
Size: 1457 1730 3099
Fragments arranged by size:
3099 1730 1457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3641 844
Size: 2797 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4173 1394
Size: 429 2350 3507
Fragments arranged by size:
3507 2350 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4781 4955 65
Size: 1183 3533 174 1396
Fragments arranged by size:
3533 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2711 4930 5117 1700
Size: 110 901 2219 187 2869
Fragments arranged by size:
2869 2219 901 187 110
BsrGI T'GTAC_A
Cuts at: 96 3501 96
Size: 3405 2881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 4346 5358 4346
Size: 1012 5274
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6286
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6286
BstXI CCAn_nnnn'nTGG
Cuts at: 3297 3297
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 2256 2549 2803 3616 3983 4368 4385 5837
Size: 284 293 254 813 367 385 17 1452
Cuts at: 5837 5923 5935 6281 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 813 385 367 346 293 284
254 86 17 12
BstZ17I GTA'TAC
Cuts at: 3005 3005
Size: 6286
BtgI C'CryG_G
Cuts at: 513 1065 2147 513
Size: 552 1082 4652
Fragments arranged by size:
4652 1082 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2123 487
Size: 683 56 897 4650
Fragments arranged by size:
4650 897 683 56
BtsI GCAGTG_nn'
Cuts at: 2216 3802 4596 4616 2216
Size: 1586 794 20 3886
Fragments arranged by size:
3886 1586 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3733 4622 6251
Size: 54 1425 50 275 18 1903 889 1629
Cuts at: 6251 8
Size: 43
Fragments arranged by size:
1903 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2106 2244 2922 2937 4216 1353
Size: 753 138 678 15 1279 3423
Fragments arranged by size:
3423 1279 753 678 138 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4873 5247 5393 387
Size: 1064 138 3284 374 146 1280
Fragments arranged by size:
3284 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2268 2360 2946 3167 3330 3436 3465
Size: 393 636 92 586 221 163 106 29
Cuts at: 3465 3546 4349 4946 5733 6001 1239
Size: 81 803 597 787 268 1524
Fragments arranged by size:
1524 803 787 636 597 586 393 268
221 163 106 92 81 29
EcoRI G'AATT_C
Cuts at: 3595 3973 3595
Size: 378 5908
EcoRV GAT'ATC
Cuts at: 2735 2735
Size: 6286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3104 3335 3104
Size: 231 6055
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 5433 1678
Size: 3755 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3338 3370 4927 4959 5001
Size: 32 135 32 1766 32 1557 32 42
Cuts at: 5001 5033 1373
Size: 32 2626
Fragments arranged by size:
2626 1766 1557 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3630 3876 669
Size: 988 281 1692 246 3079
Fragments arranged by size:
3079 1692 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3086 748
Size: 2338 3948
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 2458 3611 2458
Size: 1153 5133
MfeI C'AATT_G
Cuts at: 2159 3885 2159
Size: 1726 4560
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2205 5400 5584 1305
Size: 900 3195 184 2007
Fragments arranged by size:
3195 2007 900 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4464
Fragments arranged by size:
4464 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2865 4244 4603 4762 518
Size: 648 1699 1379 359 159 2042
Fragments arranged by size:
2042 1699 1379 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2774 3010 3507 3702 4379 5527 5772 1606
Size: 1168 236 497 195 677 1148 245 2120
Fragments arranged by size:
2120 1168 1148 677 497 245 236 195
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5734
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NsiI A_TGCA'T
Cuts at: 3212 3212
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 3210 3644 5189 1132
Size: 97 1981 434 1545 2229
Fragments arranged by size:
2229 1981 1545 434 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 5185 5185
Size: 6286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6286
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 5868 5900 4355
Size: 32 1481 32 4741
Fragments arranged by size:
4741 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2503 1613
Size: 890 5396
PsiI TTA'TAA
Cuts at: 2825 3856 2825
Size: 1031 5255
PstI C_TGCA'G
Cuts at: 830 3638 830
Size: 2808 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
PvuII CAG'CTG
Cuts at: 2774 3507 2774
Size: 733 5553
SalI G'TCGA_C
Cuts at: 1936 3628 1936
Size: 1692 4594
SapI GCTCTTCn'nnn_
Cuts at: 2106 2106
Size: 6286
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 4534 6246 4534
Size: 1712 4574
SfcI C'TryA_G
Cuts at: 826 1045 3634 4769 5450 5641 826
Size: 219 2589 1135 681 191 1471
Fragments arranged by size:
2589 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2311 4390 5291 5553 820
Size: 197 926 368 2079 901 262 1553
Fragments arranged by size:
2079 1553 926 901 368 262 197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 3644 1132
Size: 2512 3774
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6286
StyI C'CwwG_G
Cuts at: 513 1065 3725 513
Size: 552 2660 3074
Fragments arranged by size:
3074 2660 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2147 4313 4330 4483 4668 5113 325
Size: 1255 567 2166 17 153 185 445 1498
Fragments arranged by size:
2166 1498 1255 567 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3330 3501 4532
Size: 274 80 33 51 1443 1353 171 1031
Cuts at: 4532 6244 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1353 1031 274 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6246 6278 6246
Size: 32 6254
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6286
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 2115 3315 4415 2115
Size: 1200 1100 3986
Fragments arranged by size:
3986 1200 1100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI Bsu36I DraIII EcoICRI EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI