Restriction map for
pFN21AE0968
(Circular) (Six-base) MAPSORT of: pFN21AE0968.seq Check: 3562 from: 1 to: 6265
pFN21AE0968 6265 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2787 3453 3608 1937
Size: 850 666 155 4594
Fragments arranged by size:
4594 850 666 155
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 3546 4392 4765 1563
Size: 1983 846 373 3063
Fragments arranged by size:
3063 1983 846 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2013 2303 2471 2593 4328 5712 5980 1632
Size: 381 290 168 122 1735 1384 268 1917
Fragments arranged by size:
1917 1735 1384 381 290 268 168 122
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 2790 2862 5164 6141 1203
Size: 1587 72 2302 977 1327
Fragments arranged by size:
2302 1587 1327 977 72
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 3095 1166
Size: 1929 4336
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 2180
Size: 32 6233
AlwNI CAG_nnn'CTG
Cuts at: 2440 3247 4008 5580 2440
Size: 807 761 1572 3125
Fragments arranged by size:
3125 1572 807 761
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 2240 3574 3800 3952 6061 1493
Size: 747 1334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 747 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 2918 3313 3590 1870
Size: 73 975 395 277 4545
Fragments arranged by size:
4545 975 395 277 73
BamHI G'GATC_C
Cuts at: 2065 3595 3962 2065
Size: 1530 367 4368
Fragments arranged by size:
4368 1530 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3586 5041 618
Size: 325 217 627 1799 1455 1842
Fragments arranged by size:
1842 1799 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2022 2559 3584 1391
Size: 631 537 1025 4072
Fragments arranged by size:
4072 1025 631 537
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4456 4490 1903
Size: 34 50 34 2435 34 3678
Fragments arranged by size:
3678 2435 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 2296 3397 1548
Size: 748 1101 4416
Fragments arranged by size:
4416 1101 748
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4876 136
Size: 107 122 71 631 605 337 2867 1525
Fragments arranged by size:
2867 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6260 6260
Size: 6265
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 4326 5482 1790
Size: 2536 1156 2573
Fragments arranged by size:
2573 2536 1156
BmgBI CAC'GTC
Cuts at: 1318 2851 1318
Size: 1533 4732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3996 4954 442
Size: 416 53 83 399 24 2579 958 1753
Fragments arranged by size:
2579 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2983 3398 4925 1239
Size: 1106 638 415 1527 2579
Fragments arranged by size:
2579 1527 1106 638 415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2681 3173 3206 3518 4390 5255 5553 2006
Size: 675 492 33 312 872 865 298 2718
Fragments arranged by size:
2718 872 865 675 492 312 298 33
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2793 4056 493
Size: 711 1589 1263 2702
Fragments arranged by size:
2702 1589 1263 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4072 4454 5089 275
Size: 53 83 186 936 2539 382 635 1451
Fragments arranged by size:
2539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4698 5096 5370 5517 5928 4698
Size: 398 274 147 411 5035
Fragments arranged by size:
5035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 1178
Size: 30 165 30 777 30 5233
Fragments arranged by size:
5233 777 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 1186
Size: 907 5358
BseYI C'CCAG_C
Cuts at: 2962 5468 5964 2962
Size: 2506 496 3263
Fragments arranged by size:
3263 2506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5692
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2559 4326 4411 5482 2022
Size: 537 1767 85 1071 2805
Fragments arranged by size:
2805 1767 1071 537 85
BsmI GAATG_Cn'
Cuts at: 3388 3776 3869 3388
Size: 388 93 5784
Fragments arranged by size:
5784 388 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2559 3584 4326 4411 5482 1391
Size: 399 232 537 1025 742 85 1071 2174
Fragments arranged by size:
2174 1071 1025 742 537 399 232 85
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 4154 5884 4154
Size: 1730 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3620 844
Size: 2776 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4152 1394
Size: 429 2329 3507
Fragments arranged by size:
3507 2329 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2301 4760 4934 65
Size: 1183 1053 2459 174 1396
Fragments arranged by size:
2459 1396 1183 1053 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 2805 2978 4909 5096 1700
Size: 110 198 797 173 1931 187 2869
Fragments arranged by size:
2869 1931 797 198 187 173 110
BsrGI T'GTAC_A
Cuts at: 96 2895 96
Size: 2799 3466
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6265
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstXI CCAn_nnnn'nTGG
Cuts at: 2368 3124 2368
Size: 756 5509
BstYI r'GATC_y
Cuts at: 1972 2065 2454 3595 3962 4347 4364 5816 5902
Size: 93 389 1141 367 385 17 1452 86
Cuts at: 5902 5914 6260 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1141 389 385 367 346 93
86 17 12
BstZ17I GTA'TAC
Cuts at: 2788 2788
Size: 6265
Bsu36I CC'TnA_GG
Cuts at: 3248 3248
Size: 6265
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 3781 4575 4595 3781
Size: 794 20 5451
Fragments arranged by size:
5451 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 2853 3712
Size: 54 1425 50 275 18 528 495 859
Cuts at: 3712 4601 6230 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 859 528 495 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2414 3168 4195 1353
Size: 1061 754 1027 3423
Fragments arranged by size:
3423 1061 1027 754
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2903 3503 4852 5226 5372 387
Size: 1064 138 1314 600 1349 374 146 1280
Fragments arranged by size:
1349 1314 1280 1064 600 374 146 138
EcoICRI GAG'CTC
Cuts at: 2020 2557 2020
Size: 537 5728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2013 2303 2345 2471 2593 2983 3398
Size: 393 381 290 42 126 122 390 415
Cuts at: 3398 4328 4925 5712 5980 1239
Size: 930 597 787 268 1524
Fragments arranged by size:
1524 930 787 597 415 393 390 381
290 268 126 122 42
EcoO109I rG'GnC_Cy
Cuts at: 2125 2287 2343 2437 3085 3184 3237 2125
Size: 162 56 94 648 99 53 5153
Fragments arranged by size:
5153 648 162 99 94 56 53
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 6265
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 2632 5412 1678
Size: 954 2780 2531
Fragments arranged by size:
2780 2531 954
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 2744 2776 4906
Size: 32 135 32 497 32 643 32 2130
Cuts at: 4906 4938 4980 5012 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2130 643 497 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 669
Size: 988 281 1671 246 3079
Fragments arranged by size:
3079 1671 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2132 748
Size: 1384 4881
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5379 5563 1305
Size: 4074 184 2007
Fragments arranged by size:
4074 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 2855 10
Size: 54 1425 343 528 495 3420
Fragments arranged by size:
3420 1425 528 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2861 2957 3095 3393 4223 4582 4741
Size: 648 1695 96 138 298 830 359 159
Cuts at: 4741 518
Size: 2042
Fragments arranged by size:
2042 1695 830 648 359 298 159 138
96
MspA1I CmG'CkG
Cuts at: 1606 2117 2149 2941 3439 3479 3681 4358 5506
Size: 511 32 792 498 40 202 677 1148
Cuts at: 5506 5751 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 792 677 511 498 245 202
40 32
NaeI GCC'GGC
Cuts at: 2010 2980 2010
Size: 970 5295
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5713
NdeI CA'TA_TG
Cuts at: 387 2158 2508 387
Size: 1771 350 4144
Fragments arranged by size:
4144 1771 350
NgoMIV G'CCGG_C
Cuts at: 2008 2978 2008
Size: 970 5295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2866 3197 3383 3623 5168 1132
Size: 97 1637 331 186 240 1545 2229
Fragments arranged by size:
2229 1637 1545 331 240 186 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 2862 5164 2862
Size: 2302 3963
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6265
PfoI T'CCnGG_A
Cuts at: 2457 2999 3123 2457
Size: 542 124 5599
Fragments arranged by size:
5599 542 124
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2180 2212 4334 4366 5847 5879 2180
Size: 32 2122 32 1481 32 2566
Fragments arranged by size:
2566 2122 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2287 2343 3085 2287
Size: 56 742 5467
Fragments arranged by size:
5467 742 56
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PspXI vC'TCGA_Gb
Cuts at: 3313 3313
Size: 6265
PstI C_TGCA'G
Cuts at: 830 3617 830
Size: 2787 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2149 2941 3439 3479 2149
Size: 792 498 40 4935
Fragments arranged by size:
4935 792 498 40
SacI G_AGCT'C
Cuts at: 2022 2559 2022
Size: 537 5728
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SfcI C'TryA_G
Cuts at: 826 1045 3348 3613 4748 5429 5620 826
Size: 219 2303 265 1135 681 191 1471
Fragments arranged by size:
2303 1471 1135 681 265 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 2920 3592 1872
Size: 1048 672 4545
Fragments arranged by size:
4545 1048 672
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 2696 3188 3221 3313 3533
Size: 197 926 78 675 492 33 92 220
Cuts at: 3533 4369 5270 5532 820
Size: 836 901 262 1553
Fragments arranged by size:
1553 926 901 836 675 492 262 220
197 92 78 33
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3197 3623 1132
Size: 2065 426 3774
Fragments arranged by size:
3774 2065 426
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6265
StyI C'CwwG_G
Cuts at: 513 1065 2070 3081 3704 513
Size: 552 1005 1011 623 3074
Fragments arranged by size:
3074 1011 1005 623 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4292 4309 4462 4647 5092 325
Size: 1255 2712 17 153 185 445 1498
Fragments arranged by size:
2712 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2895 4511 6223
Size: 274 80 33 51 1443 918 1616 1712
Cuts at: 6223 96
Size: 138
Fragments arranged by size:
1712 1616 1443 918 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6225 6257 6225
Size: 32 6233
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 3313 1943
Size: 1370 4895
XmaI C'CCGG_G
Cuts at: 1870 2918 3590 1870
Size: 1048 672 4545
Fragments arranged by size:
4545 1048 672
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoNI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI