Restriction map for
pFN21AE0966
(Circular) (Six-base) MAPSORT of: pFN21AE0966.seq Check: 2882 from: 1 to: 6241
pFN21AE0966 6241 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4051 278
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 1937
Size: 1647 4594
Acc65I G'GTAC_C
Cuts at: 3562 3562
Size: 6241
AclI AA'CG_TT
Cuts at: 1563 4368 4741 1563
Size: 2805 373 3063
Fragments arranged by size:
3063 2805 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 2689 3011 4304 5688 5956 1632
Size: 953 104 322 1293 1384 268 1917
Fragments arranged by size:
1917 1384 1293 953 322 268 104
AfeI AGC'GCT
Cuts at: 2147 2147
Size: 6241
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 3159 5140 6117 1203
Size: 1956 1981 977 1327
Fragments arranged by size:
1981 1956 1327 977
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 2677 4970 2677
Size: 2293 3948
AleI CACnn'nnGTG
Cuts at: 1166 2086 3008 3509 1166
Size: 920 922 501 3898
Fragments arranged by size:
3898 922 920 501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6241
AlwNI CAG_nnn'CTG
Cuts at: 2360 2581 3984 5556 2360
Size: 221 1403 1572 3045
Fragments arranged by size:
3045 1572 1403 221
ApaI G_GGCC'C
Cuts at: 2369 2369
Size: 6241
ApaLI G'TGCA_C
Cuts at: 3299 4298 5454 3299
Size: 999 1156 4086
Fragments arranged by size:
4086 1156 999
ApoI r'AATT_y
Cuts at: 1493 3550 3776 3928 6037 1493
Size: 2057 226 152 2109 1697
Fragments arranged by size:
2109 2057 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4795 160
Size: 872 3763 1606
Fragments arranged by size:
3763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 2595 3113 3346 3566 1870
Size: 73 652 518 233 220 4545
Fragments arranged by size:
4545 652 518 233 220 73
BamHI G'GATC_C
Cuts at: 3571 3938 3571
Size: 367 5874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2207 2925 3562 5017
Size: 325 217 627 224 196 718 637 1455
Cuts at: 5017 618
Size: 1842
Fragments arranged by size:
1842 1455 718 637 627 325 224 217
196
BanII G_rGCy'C
Cuts at: 1391 2220 2369 2819 3560 1391
Size: 829 149 450 741 4072
Fragments arranged by size:
4072 829 741 450 149
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 6241
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4432 4466 1903
Size: 34 2495 34 3678
Fragments arranged by size:
3678 2495 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4133 5343 4133
Size: 1210 5031
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3014 4852
Size: 107 122 71 631 605 337 1005 1838
Cuts at: 4852 136
Size: 1525
Fragments arranged by size:
1838 1525 1005 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2655 2700 6236 2655
Size: 45 3536 2660
Fragments arranged by size:
3536 2660 45
BlpI GC'TnA_GC
Cuts at: 3658 3658
Size: 6241
Bme1580I G_kGCm'C
Cuts at: 1790 2369 2922 3303 4302 5458 1790
Size: 579 553 381 999 1156 2573
Fragments arranged by size:
2573 1156 999 579 553 381
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2119 3972 4930
Size: 416 53 83 399 24 702 1853 958
Cuts at: 4930 442
Size: 1753
Fragments arranged by size:
1853 1753 958 702 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2162 2603 2651 3042 4901 1239
Size: 899 24 441 48 391 1859 2579
Fragments arranged by size:
2579 1859 899 441 391 48 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2819 4366 5231 5529 2819
Size: 1547 865 298 3531
Fragments arranged by size:
3531 1547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4904 882
Size: 617 3405 2219
Fragments arranged by size:
3405 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2379 4032 493
Size: 711 1175 1653 2702
Fragments arranged by size:
2702 1653 1175 711
BsaBI GATnn'nnATC
Cuts at: 6235 6235
Size: 6241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 4048 4430 5065
Size: 53 83 186 936 479 2036 382 635
Cuts at: 5065 275
Size: 1451
Fragments arranged by size:
2036 1451 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4674 5072 5346 5493 5904 4674
Size: 398 274 147 411 5011
Fragments arranged by size:
5011 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2801 2831 3179 3209 3422
Size: 30 165 30 1398 30 348 30 213
Cuts at: 3422 3452 1178
Size: 30 3967
Fragments arranged by size:
3967 1398 348 213 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2300 3293 3392 3443 1186
Size: 838 276 993 99 51 3984
Fragments arranged by size:
3984 993 838 276 99 51
BseYI C'CCAG_C
Cuts at: 2360 2625 3357 3384 5444 5940 2360
Size: 265 732 27 2060 496 2661
Fragments arranged by size:
2661 2060 732 496 265 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 1163
Size: 573 423 5245
Fragments arranged by size:
5245 573 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3691 4452 5480 1540
Size: 275 186 1690 761 1028 2301
Fragments arranged by size:
2301 1690 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 3114 3303 4302 4387 5458 2220
Size: 894 189 999 85 1071 3003
Fragments arranged by size:
3003 1071 999 894 189 85
BsiWI C'GTAC_G
Cuts at: 2379 2379
Size: 6241
BsmI GAATG_Cn'
Cuts at: 3752 3845 3752
Size: 93 6148
BsmBI CGTCTCn'nnnn_
Cuts at: 2873 3082 2873
Size: 209 6032
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2369 2819 2922 3114 3303 3560
Size: 399 430 149 450 103 192 189 257
Cuts at: 3560 4302 4387 5458 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 430 399 257 192
189 149 103 85
BspEI T'CCGG_A
Cuts at: 5904 5904
Size: 6241
BspHI T'CATG_A
Cuts at: 2631 4130 5860 2631
Size: 1499 1730 3012
Fragments arranged by size:
3012 1730 1499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2742 2913 3525 3596 844
Size: 1898 171 612 71 3489
Fragments arranged by size:
3489 1898 612 171 71
BsrBI CCG'CTC
Cuts at: 1394 1823 2136 3433 4128 1394
Size: 429 313 1297 695 3507
Fragments arranged by size:
3507 1297 695 429 313
BsrDI GCAATG_nn'
Cuts at: 65 1248 2745 4736 4910 65
Size: 1183 1497 1991 174 1396
Fragments arranged by size:
1991 1497 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 2484 4885 5072 1700
Size: 110 244 430 2401 187 2869
Fragments arranged by size:
2869 2401 430 244 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6241
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6241
BssSI C'ACGA_G
Cuts at: 2219 2726 2889 3085 4301 5313 2219
Size: 507 163 196 1216 1012 3147
Fragments arranged by size:
3147 1216 1012 507 196 163
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2328 794
Size: 1534 4707
BstBI TT'CG_AA
Cuts at: 5045 5045
Size: 6241
BstEII G'GTnAC_C
Cuts at: 1184 3493 1184
Size: 2309 3932
BstXI CCAn_nnnn'nTGG
Cuts at: 3175 3175
Size: 6241
BstYI r'GATC_y
Cuts at: 1972 2655 2700 3571 3938 4323 4340 5792 5878
Size: 683 45 871 367 385 17 1452 86
Cuts at: 5878 5890 6236 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 871 683 385 367 346 86
45 17 12
Bsu36I CC'TnA_GG
Cuts at: 3105 3105
Size: 6241
BtgI C'CryG_G
Cuts at: 513 1065 2929 3070 3168 3222 3414 513
Size: 552 1864 141 98 54 192 3340
Fragments arranged by size:
3340 1864 552 192 141 98 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2474 487
Size: 683 56 1248 4254
Fragments arranged by size:
4254 1248 683 56
BtsI GCAGTG_nn'
Cuts at: 2176 2429 2570 3757 4551 4571 2176
Size: 253 141 1187 794 20 3846
Fragments arranged by size:
3846 1187 794 253 141 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6206
DraI TTT'AAA
Cuts at: 2971 3545 2971
Size: 574 5667
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5248 809
Size: 507 3932 1802
Fragments arranged by size:
3932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 3259 3688
Size: 54 1425 50 275 18 447 982 429
Cuts at: 3688 4577 6206 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 982 889 447 429 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3688 1537
Size: 275 1876 4090
Fragments arranged by size:
4090 1876 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2987 3246 4171 1353
Size: 1634 259 925 3423
Fragments arranged by size:
3423 1634 925 259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4828 5202 5348 387
Size: 1064 138 3239 374 146 1280
Fragments arranged by size:
3239 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2218 2218
Size: 6241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2162 2585 2603 2651 2689 3011
Size: 393 506 24 423 18 48 38 322
Cuts at: 3011 3042 4304 4901 5688 5956 1239
Size: 31 1262 597 787 268 1524
Fragments arranged by size:
1524 1262 787 597 506 423 393 322
268 48 38 31 24 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2909 2909
Size: 6241
EcoO109I rG'GnC_Cy
Cuts at: 2365 2446 2527 2365
Size: 81 81 6079
Fragments arranged by size:
6079 81 81
EcoRI G'AATT_C
Cuts at: 3550 3928 3550
Size: 378 5863
FspI TGC'GCA
Cuts at: 3152 4747 3152
Size: 1595 4646
HaeII r_GCGC'y
Cuts at: 1678 2015 2149 2605 5388 1678
Size: 337 134 456 2783 2531
Fragments arranged by size:
2783 2531 456 337 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2578 2610 2663 2695 2801
Size: 32 135 32 1006 32 53 32 106
Cuts at: 2801 2833 2870 2902 3179 3211 3422 3454 4882
Size: 32 37 32 277 32 211 32 1428
Cuts at: 4882 4914 4956 4988 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1428 1006 277 211 135 106 53
42 37 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3460 3585 3831 669
Size: 988 281 1522 125 246 3079
Fragments arranged by size:
3079 1522 988 281 246 125
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6241
HpaI GTT'AAC
Cuts at: 3831 3831
Size: 6241
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 6241
KpnI G_GTAC'C
Cuts at: 3566 3566
Size: 6241
MfeI C'AATT_G
Cuts at: 3840 3840
Size: 6241
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2793 3255 5355 5539 1305
Size: 1488 462 2100 184 2007
Fragments arranged by size:
2100 2007 1488 462 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 3261 10
Size: 54 1425 343 447 982 2990
Fragments arranged by size:
2990 1425 982 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2086 2913 3008 3158 3509 4199 4558
Size: 648 920 827 95 150 351 690 359
Cuts at: 4558 4717 518
Size: 159 2042
Fragments arranged by size:
2042 920 827 690 648 359 351 159
150 95
MspA1I CmG'CkG
Cuts at: 1606 2578 3416 3657 4334 5482 5727 1606
Size: 972 838 241 677 1148 245 2120
Fragments arranged by size:
2120 1148 972 838 677 245 241
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 6241
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 6241
NcoI C'CATG_G
Cuts at: 513 1065 3168 513
Size: 552 2103 3586
Fragments arranged by size:
3586 2103 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6241
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 6241
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6241
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NspI r_CATG'y
Cuts at: 1132 1229 3163 3238 3599 5144 1132
Size: 97 1934 75 361 1545 2229
Fragments arranged by size:
2229 1934 1545 361 97 75
PasI CC'CwG_GG
Cuts at: 1348 2016 2531 1348
Size: 668 515 5058
Fragments arranged by size:
5058 668 515
PciI A'CATG_T
Cuts at: 3159 5140 3159
Size: 1981 4260
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2360 1108
Size: 1252 4989
PfoI T'CCnGG_A
Cuts at: 2667 2667
Size: 6241
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 5823 5855 4310
Size: 32 1481 32 4696
Fragments arranged by size:
4696 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 2527
Size: 6241
PshAI GACnn'nnGTC
Cuts at: 1613 3500 1613
Size: 1887 4354
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PspOMI G'GGCC_C
Cuts at: 2365 2365
Size: 6241
PspXI vC'TCGA_Gb
Cuts at: 3113 3113
Size: 6241
PstI C_TGCA'G
Cuts at: 830 2577 3214 3288 3593 830
Size: 1747 637 74 305 3478
Fragments arranged by size:
3478 1747 637 305 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
PvuII CAG'CTG
Cuts at: 2578 2578
Size: 6241
SacI G_AGCT'C
Cuts at: 2220 2220
Size: 6241
SacII CC_GC'GG
Cuts at: 3417 3417
Size: 6241
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SapI GCTCTTCn'nnn_
Cuts at: 2987 2987
Size: 6241
SbfI CC_TGCA'GG
Cuts at: 3593 3593
Size: 6241
ScaI AGT'ACT
Cuts at: 4489 6201 4489
Size: 1712 4529
SfcI C'TryA_G
Cuts at: 826 1045 2573 2738 3210 3284 3589 4724 5405
Size: 219 1528 165 472 74 305 1135 681
Cuts at: 5405 5596 826
Size: 191 1471
Fragments arranged by size:
1528 1471 1135 681 472 305 219 191
165 74
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 6241
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6241
SmaI CCC'GGG
Cuts at: 1872 3348 3568 1872
Size: 1476 220 4545
Fragments arranged by size:
4545 1476 220
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2834 3113 4345 5246 5508 820
Size: 197 926 891 279 1232 901 262 1553
Fragments arranged by size:
1553 1232 926 901 891 279 262 197
SnaBI TAC'GTA
Cuts at: 493 4032 493
Size: 3539 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6241
SphI G_CATG'C
Cuts at: 1132 3599 1132
Size: 2467 3774
SspI AAT'ATT
Cuts at: 5 52 4165 5
Size: 47 4113 2081
Fragments arranged by size:
4113 2081 47
StuI AGG'CCT
Cuts at: 1517 2895 1517
Size: 1378 4863
StyI C'CwwG_G
Cuts at: 513 1065 3168 3680 513
Size: 552 2103 512 3074
Fragments arranged by size:
3074 2103 552 512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2008 3083 3445 4268 4285 4438 4623
Size: 1255 428 1075 362 823 17 153 185
Cuts at: 4623 5068 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1075 823 445 428 362 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 3231 4487
Size: 274 80 33 51 1443 324 930 1256
Cuts at: 4487 6199 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1256 930 324 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6201 6233 6201
Size: 32 6209
Tth111I GACn'n_nGTC
Cuts at: 1316 2884 1316
Size: 1568 4673
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 2528 2113
Size: 415 5826
XhoI C'TCGA_G
Cuts at: 1943 3113 1943
Size: 1170 5071
XmaI C'CCGG_G
Cuts at: 1870 3346 3566 1870
Size: 1476 220 4545
Fragments arranged by size:
4545 1476 220
XmnI GAAnn'nnTTC
Cuts at: 4370 4370
Size: 6241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4049 276
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BstZ17I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PsrI RsrII SanDI SexAI SfiI
SrfI SwaI