Restriction map for
pFN21AE0964
(Circular) (Six-base) MAPSORT of: pFN21AE0964.seq Check: 7651 from: 1 to: 6229
pFN21AE0964 6229 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4039 278
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3572 1937
Size: 1635 4594
Acc65I G'GTAC_C
Cuts at: 2411 3550 2411
Size: 1139 5090
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2786 4292 5676 5944 1632
Size: 1154 1506 1384 268 1917
Fragments arranged by size:
1917 1506 1384 1154 268
AfeI AGC'GCT
Cuts at: 3112 3112
Size: 6229
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 2176 5128 6105 1203
Size: 973 2952 977 1327
Fragments arranged by size:
2952 1327 977 973
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 4958 4958
Size: 6229
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6229
AlwNI CAG_nnn'CTG
Cuts at: 2764 2859 3972 5544 2764
Size: 95 1113 1572 3449
Fragments arranged by size:
3449 1572 1113 95
ApaLI G'TGCA_C
Cuts at: 4286 5442 4286
Size: 1156 5073
ApoI r'AATT_y
Cuts at: 1493 2228 2301 2333 2937 3538 3764 3916 6025
Size: 735 73 32 604 601 226 152 2109
Cuts at: 6025 1493
Size: 1697
Fragments arranged by size:
2109 1697 735 604 601 226 152 73
32
AseI AT'TA_AT
Cuts at: 160 1032 4783 160
Size: 872 3751 1606
Fragments arranged by size:
3751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 3554 1870
Size: 73 1611 4545
Fragments arranged by size:
4545 1611 73
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2411 3550 5005 618
Size: 325 217 627 624 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 624 325 217
BanII G_rGCy'C
Cuts at: 1391 2817 2842 3548 1391
Size: 1426 25 706 4072
Fragments arranged by size:
4072 1426 706 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3208 928
Size: 971 1309 3949
Fragments arranged by size:
3949 1309 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4420 4454 1903
Size: 34 50 34 2399 34 3678
Fragments arranged by size:
3678 2399 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2456 4121 5331 2456
Size: 1665 1210 3354
Fragments arranged by size:
3354 1665 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4840 136
Size: 107 122 71 631 605 3168 1525
Fragments arranged by size:
3168 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2394 3341 6224 2394
Size: 947 2883 2399
Fragments arranged by size:
2883 2399 947
BlpI GC'TnA_GC
Cuts at: 3646 3646
Size: 6229
Bme1580I G_kGCm'C
Cuts at: 1790 4290 5446 1790
Size: 2500 1156 2573
Fragments arranged by size:
2573 2500 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2105 2815 3424
Size: 416 53 83 399 24 688 710 609
Cuts at: 3424 3960 4918 442
Size: 536 958 1753
Fragments arranged by size:
1753 958 710 688 609 536 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 4889 1239
Size: 897 2753 2579
Fragments arranged by size:
2753 2579 897
Bpu10I CC'TnA_GC
Cuts at: 3183 3183
Size: 6229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3207 4354 5219 5517 2915
Size: 292 1147 865 298 3627
Fragments arranged by size:
3627 1147 865 298 292
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4892 882
Size: 617 3393 2219
Fragments arranged by size:
3393 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 3346 6223 3346
Size: 2877 3352
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4036 4418 5053 275
Size: 53 83 186 936 2503 382 635 1451
Fragments arranged by size:
2503 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4662 5060 5334 5481 5892 4662
Size: 398 274 147 411 4999
Fragments arranged by size:
4999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6004
Fragments arranged by size:
6004 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3161 1186
Size: 1975 4254
BseYI C'CCAG_C
Cuts at: 5432 5928 5432
Size: 496 5733
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2804 3158 1163
Size: 573 1068 354 4234
Fragments arranged by size:
4234 1068 573 354
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3679 4440 5468 1540
Size: 275 186 1678 761 1028 2301
Fragments arranged by size:
2301 1678 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2817 2890 4290 4375 5446 2817
Size: 73 1400 85 1071 3600
Fragments arranged by size:
3600 1400 1071 85 73
BsmI GAATG_Cn'
Cuts at: 3740 3833 3740
Size: 93 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2817 2842 2890 3548 4290 4375 5446
Size: 399 1027 25 48 658 742 85 1071
Cuts at: 5446 1391
Size: 2174
Fragments arranged by size:
2174 1071 1027 742 658 399 85 48
25
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3584 844
Size: 2740 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4116 1394
Size: 429 2293 3507
Fragments arranged by size:
3507 2293 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4724 4898 65
Size: 1183 3476 174 1396
Fragments arranged by size:
3476 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4873 5060 1700
Size: 110 3063 187 2869
Fragments arranged by size:
3063 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6229
BssSI C'ACGA_G
Cuts at: 4289 5301 4289
Size: 1012 5217
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6229
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6229
BstYI r'GATC_y
Cuts at: 1972 2318 2394 2706 3341 3559 3926 4311 4328
Size: 346 76 312 635 218 367 385 17
Cuts at: 4328 5780 5866 5878 6224 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 635 385 367 346 346 312
218 86 76 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5677
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5490
Fragments arranged by size:
5490 683 56
BtsI GCAGTG_nn'
Cuts at: 3745 4539 4559 3745
Size: 794 20 5415
Fragments arranged by size:
5415 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 2419 3533 2419
Size: 1114 5115
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3676 4565 6194
Size: 54 1425 50 275 18 1846 889 1629
Cuts at: 6194 8
Size: 43
Fragments arranged by size:
1846 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3676 1537
Size: 275 1864 4090
Fragments arranged by size:
4090 1864 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3441 4159 1353
Size: 2088 718 3423
Fragments arranged by size:
3423 2088 718
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4816 5190 5336 387
Size: 1064 138 3227 374 146 1280
Fragments arranged by size:
3227 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2815 2815
Size: 6229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2786 4292 4889 5676 5944 1239
Size: 393 504 650 1506 597 787 268 1524
Fragments arranged by size:
1524 1506 787 650 597 504 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2625 2625
Size: 6229
EcoO109I rG'GnC_Cy
Cuts at: 2770 2770
Size: 6229
EcoRI G'AATT_C
Cuts at: 2301 2333 3538 3916 2301
Size: 32 1205 378 4614
Fragments arranged by size:
4614 1205 378 32
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 3114 5376 1678
Size: 1436 2262 2531
Fragments arranged by size:
2531 2262 1436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4870 4902 4944 4976 1373
Size: 32 135 32 3298 32 42 32 2626
Fragments arranged by size:
3298 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2427 3061 3573 3819 669
Size: 988 281 489 634 512 246 3079
Fragments arranged by size:
3079 988 634 512 489 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KpnI G_GTAC'C
Cuts at: 2415 3554 2415
Size: 1139 5090
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2436 2787 5343 5527 1305
Size: 1131 351 2556 184 2007
Fragments arranged by size:
2556 2007 1131 351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4407
Fragments arranged by size:
4407 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2481 2499 4187 4546 4705 518
Size: 648 1315 18 1688 359 159 2042
Fragments arranged by size:
2042 1688 1315 648 359 159 18
MspA1I CmG'CkG
Cuts at: 1606 3645 4322 5470 5715 1606
Size: 2039 677 1148 245 2120
Fragments arranged by size:
2120 2039 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5677
NdeI CA'TA_TG
Cuts at: 387 2902 387
Size: 2515 3714
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 2180 2725 3587 5132 1132
Size: 97 951 545 862 1545 2229
Fragments arranged by size:
2229 1545 951 862 545 97
PasI CC'CwG_GG
Cuts at: 1348 2751 1348
Size: 1403 4826
PciI A'CATG_T
Cuts at: 2176 5128 2176
Size: 2952 3277
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2878 1108
Size: 1770 4459
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 5811 5843 4298
Size: 32 1481 32 4684
Fragments arranged by size:
4684 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2770 2770
Size: 6229
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PstI C_TGCA'G
Cuts at: 830 3581 830
Size: 2751 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
SacI G_AGCT'C
Cuts at: 2817 2817
Size: 6229
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 4477 6189 4477
Size: 1712 4517
SexAI A'CCwGG_T
Cuts at: 2544 2544
Size: 6229
SfcI C'TryA_G
Cuts at: 826 1045 2575 3577 4712 5393 5584 826
Size: 219 1530 1002 1135 681 191 1471
Fragments arranged by size:
1530 1471 1135 1002 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 3556 1872
Size: 1684 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2930 3222 4333 5234 5496 820
Size: 197 926 987 292 1111 901 262 1553
Fragments arranged by size:
1553 1111 987 926 901 292 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 2725 3587 1132
Size: 1593 862 3774
Fragments arranged by size:
3774 1593 862
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6229
StyI C'CwwG_G
Cuts at: 513 1065 3668 513
Size: 552 2603 3074
Fragments arranged by size:
3074 2603 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4256 4273 4426 4611 5056 325
Size: 1255 2676 17 153 185 445 1498
Fragments arranged by size:
2676 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2461 4475 6187
Size: 274 80 33 51 1443 484 2014 1712
Cuts at: 6187 96
Size: 138
Fragments arranged by size:
2014 1712 1443 484 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6189 6221 6189
Size: 32 6197
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6229
XbaI T'CTAG_A
Cuts at: 3565 3565
Size: 6229
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 3554 1870
Size: 1684 4545
XmnI GAAnn'nnTTC
Cuts at: 2490 4358 2490
Size: 1868 4361
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4037 276
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI