Restriction map for
pFN21AE0957
(Circular) (Six-base) MAPSORT of: pFN21AE0957.seq Check: 5294 from: 1 to: 6184
pFN21AE0957 6184 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2167 2167
Size: 6184
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3994 278
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2419 2551 3527 1937
Size: 482 132 976 4594
Fragments arranged by size:
4594 976 482 132
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 2579 3258 4247 5631 5899 1632
Size: 470 477 679 989 1384 268 1917
Fragments arranged by size:
1917 1384 989 679 477 470 268
AfeI AGC'GCT
Cuts at: 2028 2028
Size: 6184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 5083 6060 1203
Size: 3880 977 1327
Fragments arranged by size:
3880 1327 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6184
AlwNI CAG_nnn'CTG
Cuts at: 3927 5499 3927
Size: 1572 4612
ApaLI G'TGCA_C
Cuts at: 4241 5397 4241
Size: 1156 5028
ApoI r'AATT_y
Cuts at: 1493 2715 3493 3719 3871 5980 1493
Size: 1222 778 226 152 2109 1697
Fragments arranged by size:
2109 1697 1222 778 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 3509 1870
Size: 73 67 1499 4545
Fragments arranged by size:
4545 1499 73 67
BamHI G'GATC_C
Cuts at: 3514 3881 3514
Size: 367 5817
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3268 3505 4960 618
Size: 325 217 627 1481 237 1455 1842
Fragments arranged by size:
1842 1481 1455 627 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2070 2834 3503 1391
Size: 679 764 669 4072
Fragments arranged by size:
4072 764 679 669
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2215 3449 3477 928
Size: 971 316 1234 28 3635
Fragments arranged by size:
3635 1234 971 316 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4375 4409 1903
Size: 34 2438 34 3678
Fragments arranged by size:
3678 2438 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3344 4076 5286 3344
Size: 732 1210 4242
Fragments arranged by size:
4242 1210 732
BclI T'GATC_A
Cuts at: 1548 2181 2880 1548
Size: 633 699 4852
Fragments arranged by size:
4852 699 633
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4795 136
Size: 107 122 71 631 605 3123 1525
Fragments arranged by size:
3123 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2509 6179 2509
Size: 3670 2514
BlpI GC'TnA_GC
Cuts at: 2023 3601 2023
Size: 1578 4606
Bme1580I G_kGCm'C
Cuts at: 1790 4245 5401 1790
Size: 2455 1156 2573
Fragments arranged by size:
2573 2455 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3208 3915 4873
Size: 416 53 83 399 24 1791 707 958
Cuts at: 4873 442
Size: 1753
Fragments arranged by size:
1791 1753 958 707 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2665 4844 1239
Size: 1426 2179 2579
Fragments arranged by size:
2579 2179 1426
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2202 4309 5174 5472 2202
Size: 2107 865 298 2914
Fragments arranged by size:
2914 2107 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2440 4847 882
Size: 617 941 2407 2219
Fragments arranged by size:
2407 2219 941 617
BsaAI yAC'GTr
Cuts at: 493 1204 3975 493
Size: 711 2771 2702
Fragments arranged by size:
2771 2702 711
BsaBI GATnn'nnATC
Cuts at: 6178 6178
Size: 6184
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3991 4373 5008 275
Size: 53 83 186 936 2458 382 635 1451
Fragments arranged by size:
2458 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4617 5015 5289 5436 5847 4617
Size: 398 274 147 411 4954
Fragments arranged by size:
4954 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3219 3249 1178
Size: 30 165 30 1816 30 4113
Fragments arranged by size:
4113 1816 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2668 3254 1186
Size: 1482 586 4116
Fragments arranged by size:
4116 1482 586
BseYI C'CCAG_C
Cuts at: 5387 5883 5387
Size: 496 5688
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 1163
Size: 573 543 5068
Fragments arranged by size:
5068 573 543
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 3634 4395 5423 1540
Size: 275 186 60 1573 761 1028 2301
Fragments arranged by size:
2301 1573 1028 761 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 2070 2429 4245 4330 5401 2070
Size: 359 1816 85 1071 2853
Fragments arranged by size:
2853 1816 1071 359 85
BsmI GAATG_Cn'
Cuts at: 2218 2264 3695 3788 2218
Size: 46 1431 93 4614
Fragments arranged by size:
4614 1431 93 46
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2429 2834 3503 4245 4330 5401
Size: 399 280 359 405 669 742 85 1071
Cuts at: 5401 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 669 405 399 359 280
85
BspEI T'CCGG_A
Cuts at: 5847 5847
Size: 6184
BspHI T'CATG_A
Cuts at: 2821 4073 5803 2821
Size: 1252 1730 3202
Fragments arranged by size:
3202 1730 1252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2129 2167 2962 3539 844
Size: 1285 38 795 577 3489
Fragments arranged by size:
3489 1285 795 577 38
BsrBI CCG'CTC
Cuts at: 1394 1823 3413 4071 1394
Size: 429 1590 658 3507
Fragments arranged by size:
3507 1590 658 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2760 4679 4853 65
Size: 1183 1512 1919 174 1396
Fragments arranged by size:
1919 1512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4828 5015 1700
Size: 110 3018 187 2869
Fragments arranged by size:
3018 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 4244 5256 4244
Size: 1012 5172
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6184
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6184
BstXI CCAn_nnnn'nTGG
Cuts at: 2344 3345 2344
Size: 1001 5183
BstYI r'GATC_y
Cuts at: 1972 2509 2537 3277 3514 3881 4266 4283 5735
Size: 537 28 740 237 367 385 17 1452
Cuts at: 5735 5821 5833 6179 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 740 537 385 367 346 237
86 28 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2337 2868 513
Size: 552 1272 531 3829
Fragments arranged by size:
3829 1272 552 531
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2405 487
Size: 683 56 1179 4266
Fragments arranged by size:
4266 1179 683 56
BtsI GCAGTG_nn'
Cuts at: 2291 3700 4494 4514 2291
Size: 1409 794 20 3961
Fragments arranged by size:
3961 1409 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6149
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5191 809
Size: 507 3875 1802
Fragments arranged by size:
3875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2346 3631
Size: 54 1425 50 275 18 228 288 1285
Cuts at: 3631 4520 6149 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1285 889 288 275 228 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 3631 1537
Size: 275 246 1573 4090
Fragments arranged by size:
4090 1573 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 3435 4114 1353
Size: 2082 679 3423
Fragments arranged by size:
3423 2082 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2021 4771 5145 5291 387
Size: 1064 138 432 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 432 374 146 138
EcoICRI GAG'CTC
Cuts at: 2068 2068
Size: 6184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2579 2665 3258 4247 4844 5631
Size: 393 470 477 86 593 989 597 787
Cuts at: 5631 5899 1239
Size: 268 1524
Fragments arranged by size:
1524 989 787 597 593 477 470 393
268 86
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 6184
FspI TGC'GCA
Cuts at: 4690 4690
Size: 6184
HaeII r_GCGC'y
Cuts at: 1678 2018 2030 2894 5331 1678
Size: 340 12 864 2437 2531
Fragments arranged by size:
2531 2437 864 340 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2963 2995 3159 3191 3318
Size: 32 135 32 1391 32 164 32 127
Cuts at: 3318 3350 3436 3468 4825 4857 4899 4931 1373
Size: 32 86 32 1357 32 42 32 2626
Fragments arranged by size:
2626 1391 1357 164 135 127 86 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2420 3528 3774 669
Size: 988 281 482 1108 246 3079
Fragments arranged by size:
3079 1108 988 482 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 2574 2898 3248 3783 2574
Size: 324 350 535 4975
Fragments arranged by size:
4975 535 350 324
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2262 2327 3205 5298 5482 1305
Size: 957 65 878 2093 184 2007
Fragments arranged by size:
2093 2007 957 878 184 65
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2348 10
Size: 54 1425 343 516 3846
Fragments arranged by size:
3846 1425 516 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4142 4501 4660 518
Size: 648 2976 359 159 2042
Fragments arranged by size:
2976 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2057 3600 4277 5425 5670 1606
Size: 451 1543 677 1148 245 2120
Fragments arranged by size:
2120 1543 1148 677 451 245
NcoI C'CATG_G
Cuts at: 513 1065 2337 2868 513
Size: 552 1272 531 3829
Fragments arranged by size:
3829 1272 552 531
NdeI CA'TA_TG
Cuts at: 387 2925 387
Size: 2538 3646
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 2058 3631 2058
Size: 1573 4611
NspI r_CATG'y
Cuts at: 1132 1229 2142 2412 3542 5087 1132
Size: 97 913 270 1130 1545 2229
Fragments arranged by size:
2229 1545 1130 913 270 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6184
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2330 1108
Size: 1222 4962
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 2811 4253 4285 5766 5798 2779
Size: 32 1442 32 1481 32 3165
Fragments arranged by size:
3165 1481 1442 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6184
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PstI C_TGCA'G
Cuts at: 830 3536 830
Size: 2706 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
SacI G_AGCT'C
Cuts at: 2070 2070
Size: 6184
SalI G'TCGA_C
Cuts at: 1936 2418 3526 1936
Size: 482 1108 4594
Fragments arranged by size:
4594 1108 482
SapI GCTCTTCn'nnn_
Cuts at: 3435 3435
Size: 6184
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 4432 6144 4432
Size: 1712 4472
SfcI C'TryA_G
Cuts at: 826 1045 2431 3532 4667 5348 5539 826
Size: 219 1386 1101 1135 681 191 1471
Fragments arranged by size:
1471 1386 1135 1101 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6184
SmaI CCC'GGG
Cuts at: 1872 2012 3511 1872
Size: 140 1499 4545
Fragments arranged by size:
4545 1499 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2217 4288 5189 5451 820
Size: 197 926 274 2071 901 262 1553
Fragments arranged by size:
2071 1553 926 901 274 262 197
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 2412 3542 1132
Size: 1280 1130 3774
Fragments arranged by size:
3774 1280 1130
SspI AAT'ATT
Cuts at: 5 52 2673 4108 5
Size: 47 2621 1435 2081
Fragments arranged by size:
2621 2081 1435 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6184
StyI C'CwwG_G
Cuts at: 513 1065 2337 2752 2868 3623 513
Size: 552 1272 415 116 755 3074
Fragments arranged by size:
3074 1272 755 552 415 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4211 4228 4381 4566 5011 325
Size: 1255 2631 17 153 185 445 1498
Fragments arranged by size:
2631 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4430 6142 96
Size: 274 80 33 51 1443 2453 1712 138
Fragments arranged by size:
2453 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6144 6176 6144
Size: 32 6152
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6184
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3106 3106
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 2010 3509 1870
Size: 140 1499 4545
Fragments arranged by size:
4545 1499 140
XmnI GAAnn'nnTTC
Cuts at: 2672 4313 2672
Size: 1641 4543
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3992 276
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI