Restriction map for
pFN21AE0955
(Circular) (Six-base) MAPSORT of: pFN21AE0955.seq Check: 9396 from: 1 to: 6154
pFN21AE0955 6154 bp
With 182 enzymes: SgfI *
March 28, 2012 12:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3964 278
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3419 3497 1937
Size: 1482 78 4594
Fragments arranged by size:
4594 1482 78
Acc65I G'GTAC_C
Cuts at: 3475 3475
Size: 6154
AclI AA'CG_TT
Cuts at: 1563 4281 4654 1563
Size: 2718 373 3063
Fragments arranged by size:
3063 2718 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3262 4217 5601 5869 1632
Size: 1630 955 1384 268 1917
Fragments arranged by size:
1917 1630 1384 955 268
AfeI AGC'GCT
Cuts at: 2342 2342
Size: 6154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5957
AflIII A'CryG_T
Cuts at: 1203 2676 5053 6030 1203
Size: 1473 2377 977 1327
Fragments arranged by size:
2377 1473 1327 977
AgeI A'CCGG_T
Cuts at: 4985 4985
Size: 6154
AhdI GACnn_n'nnGTC
Cuts at: 3415 4883 3415
Size: 1468 4686
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6154
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2453 2485 3053 3085 3282 3314 2453
Size: 32 568 32 197 32 5293
Fragments arranged by size:
5293 568 197 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2752 3897 5469 2752
Size: 1145 1572 3437
Fragments arranged by size:
3437 1572 1145
ApaI G_GGCC'C
Cuts at: 2866 2866
Size: 6154
ApaLI G'TGCA_C
Cuts at: 4211 5367 4211
Size: 1156 4998
ApoI r'AATT_y
Cuts at: 1493 3463 3689 3841 5950 1493
Size: 1970 226 152 2109 1697
Fragments arranged by size:
2109 1970 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 2044 2191 2270 2600 3024 3479 1870
Size: 73 101 147 79 330 424 455 4545
Fragments arranged by size:
4545 455 424 330 147 101 79 73
BamHI G'GATC_C
Cuts at: 3484 3851 3484
Size: 367 5787
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 3475 4930 618
Size: 325 217 627 351 1337 1455 1842
Fragments arranged by size:
1842 1455 1337 627 351 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2079 2198 2829 2866 2874 2943 3473
Size: 654 34 119 631 37 8 69 530
Cuts at: 3473 1391
Size: 4072
Fragments arranged by size:
4072 654 631 530 119 69 37 34
8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4345 4379 1903
Size: 34 2408 34 3678
Fragments arranged by size:
3678 2408 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4046 5256 4046
Size: 1210 4944
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 2115 2136
Size: 107 122 71 631 605 368 75 21
Cuts at: 2136 4765 136
Size: 2629 1525
Fragments arranged by size:
2629 1525 631 605 368 122 107 75
71 21
BglII A'GATC_T
Cuts at: 6149 6149
Size: 6154
BlpI GC'TnA_GC
Cuts at: 3571 3571
Size: 6154
Bme1580I G_kGCm'C
Cuts at: 1790 2601 2866 3356 4215 5371 1790
Size: 811 265 490 859 1156 2573
Fragments arranged by size:
2573 1156 859 811 490 265
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3885 4843 442
Size: 416 53 83 399 24 2468 958 1753
Fragments arranged by size:
2468 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 2530 3176 4814 1239
Size: 1202 89 646 1638 2579
Fragments arranged by size:
2579 1638 1202 646 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2765 3157 3358 4279 5144 5442 2765
Size: 392 201 921 865 298 3477
Fragments arranged by size:
3477 921 865 392 298 201
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2417 4817 882
Size: 617 918 2400 2219
Fragments arranged by size:
2400 2219 918 617
BsaAI yAC'GTr
Cuts at: 493 1204 3945 493
Size: 711 2741 2702
Fragments arranged by size:
2741 2702 711
BsaBI GATnn'nnATC
Cuts at: 6148 6148
Size: 6154
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2181 3961 4343 4978
Size: 53 83 186 936 648 1780 382 635
Cuts at: 4978 275
Size: 1451
Fragments arranged by size:
1780 1451 936 648 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4587 4985 5259 5406 5817 4587
Size: 398 274 147 411 4924
Fragments arranged by size:
4924 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2696 2726 3053 3083 3379
Size: 30 165 30 1293 30 327 30 296
Cuts at: 3379 3409 1178
Size: 30 3923
Fragments arranged by size:
3923 1293 327 296 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2109 2241 2933 3321 3439 1186
Size: 923 132 692 388 118 3901
Fragments arranged by size:
3901 923 692 388 132 118
BseYI C'CCAG_C
Cuts at: 2281 3010 3237 3346 5357 5853 2281
Size: 729 227 109 2011 496 2582
Fragments arranged by size:
2582 2011 729 496 227 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5581
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3423 3604 4365 5393 1540
Size: 275 186 1422 181 761 1028 2301
Fragments arranged by size:
2301 1422 1028 761 275 186 181
BsiHKAI G_wGCw'C
Cuts at: 2198 2874 2943 4215 4300 5371 2198
Size: 676 69 1272 85 1071 2981
Fragments arranged by size:
2981 1272 1071 676 85 69
BsmI GAATG_Cn'
Cuts at: 3665 3758 3665
Size: 93 6061
BsmBI CGTCTCn'nnnn_
Cuts at: 3312 3312
Size: 6154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2079 2198 2601 2829 2866 2874
Size: 399 255 34 119 403 228 37 8
Cuts at: 2874 2943 3356 3473 4215 4300 5371 1391
Size: 69 413 117 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 413 403 399 255 228
119 117 85 69 37 34 8
BspEI T'CCGG_A
Cuts at: 5817 5817
Size: 6154
BspHI T'CATG_A
Cuts at: 4043 5773 4043
Size: 1730 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2760 3509 844
Size: 1916 749 3489
Fragments arranged by size:
3489 1916 749
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 4041 1394
Size: 429 187 2031 3507
Fragments arranged by size:
3507 2031 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 2389 4649 4823 65
Size: 1183 1141 2260 174 1396
Fragments arranged by size:
2260 1396 1183 1141 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 4798 4985 1700
Size: 110 331 2657 187 2869
Fragments arranged by size:
2869 2657 331 187 110
BsrGI T'GTAC_A
Cuts at: 96 2673 96
Size: 2577 3577
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6154
BssSI C'ACGA_G
Cuts at: 4214 5226 4214
Size: 1012 5142
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2494 794
Size: 1700 4454
BstBI TT'CG_AA
Cuts at: 4958 4958
Size: 6154
BstEII G'GTnAC_C
Cuts at: 1184 2302 1184
Size: 1118 5036
BstYI r'GATC_y
Cuts at: 1972 3484 3851 4236 4253 5705 5791 5803 6149
Size: 1512 367 385 17 1452 86 12 346
Cuts at: 6149 1972
Size: 1977
Fragments arranged by size:
1977 1512 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2169 513
Size: 552 1104 4498
Fragments arranged by size:
4498 1104 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3118 3238 487
Size: 683 56 1892 120 3403
Fragments arranged by size:
3403 1892 683 120 56
BtsI GCAGTG_nn'
Cuts at: 2509 2769 2806 3670 4464 4484 2509
Size: 260 37 864 794 20 4179
Fragments arranged by size:
4179 864 794 260 37 20
ClaI AT'CG_AT
Cuts at: 1554 2907 1554
Size: 1353 4801
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6119
DraI TTT'AAA
Cuts at: 3458 3458
Size: 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2774 5161 809
Size: 507 1458 2387 1802
Fragments arranged by size:
2387 1802 1458 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3601 4490 6119
Size: 54 1425 50 275 18 1771 889 1629
Cuts at: 6119 8
Size: 43
Fragments arranged by size:
1771 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3601 1537
Size: 275 1789 4090
Fragments arranged by size:
4090 1789 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2693 3275 4084 1353
Size: 1340 582 809 3423
Fragments arranged by size:
3423 1340 809 582
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2100 2106 2163 2238 4741 5115
Size: 1064 138 511 6 57 75 2503 374
Cuts at: 5115 5261 387
Size: 146 1280
Fragments arranged by size:
2503 1280 1064 511 374 146 138 75
57 6
EcoICRI GAG'CTC
Cuts at: 2196 2872 2941 2196
Size: 676 69 5409
Fragments arranged by size:
5409 676 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 2530 3176 3262 4217 4814 5601
Size: 393 809 89 646 86 955 597 787
Cuts at: 5601 5869 1239
Size: 268 1524
Fragments arranged by size:
1524 955 809 787 646 597 393 268
89 86
EcoNI CCTnn'n_nnAGG
Cuts at: 2025 2025
Size: 6154
EcoO109I rG'GnC_Cy
Cuts at: 2187 2240 2863 3342 2187
Size: 53 623 479 4999
Fragments arranged by size:
4999 623 479 53
EcoRI G'AATT_C
Cuts at: 3463 3841 3463
Size: 378 5776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2585 2585
Size: 6154
FspI TGC'GCA
Cuts at: 3201 4660 3201
Size: 1459 4695
FspAI rTGC'GCAy
Cuts at: 3201 3201
Size: 6154
HaeII r_GCGC'y
Cuts at: 1678 2344 5301 1678
Size: 666 2957 2531
Fragments arranged by size:
2957 2531 666
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2882 2900 2914 2932 3070
Size: 32 135 32 1310 18 14 18 138
Cuts at: 3070 3102 3401 3433 4795 4827 4869 4901 1373
Size: 32 299 32 1362 32 42 32 2626
Fragments arranged by size:
2626 1362 1310 299 138 135 42 32
32 32 32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2646 3420 3498 3744 669
Size: 988 281 708 774 78 246 3079
Fragments arranged by size:
3079 988 774 708 281 246 78
HindIII A'AGCT_T
Cuts at: 748 3381 748
Size: 2633 3521
HpaI GTT'AAC
Cuts at: 3744 3744
Size: 6154
KpnI G_GTAC'C
Cuts at: 3479 3479
Size: 6154
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3325 3422 5268 5452 1305
Size: 2020 97 1846 184 2007
Fragments arranged by size:
2020 2007 1846 184 97
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4332
Fragments arranged by size:
4332 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2372 3005 4112 4471 4630 518
Size: 648 1206 633 1107 359 159 2042
Fragments arranged by size:
2042 1206 1107 648 633 359 159
MspA1I CmG'CkG
Cuts at: 1606 2220 2235 2295 2921 2989 3241 3350 3570
Size: 614 15 60 626 68 252 109 220
Cuts at: 3570 4247 5395 5640 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 626 614 252 245 220
109 68 60 15
NcoI C'CATG_G
Cuts at: 513 1065 2169 513
Size: 552 1104 4498
Fragments arranged by size:
4498 1104 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6154
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6154
NotI GC'GGCC_GC
Cuts at: 3601 3601
Size: 6154
NspI r_CATG'y
Cuts at: 1132 1229 2680 3512 5057 1132
Size: 97 1451 832 1545 2229
Fragments arranged by size:
2229 1545 1451 832 97
PasI CC'CwG_GG
Cuts at: 1348 2036 2716 2966 1348
Size: 688 680 250 4536
Fragments arranged by size:
4536 688 680 250
PciI A'CATG_T
Cuts at: 2676 5053 2676
Size: 2377 3777
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2353 2992 1108
Size: 1245 639 4270
Fragments arranged by size:
4270 1245 639
PfoI T'CCnGG_A
Cuts at: 2457 2457
Size: 6154
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3053 3085 4223 4255 5736 5768 3053
Size: 32 1138 32 1481 32 3439
Fragments arranged by size:
3439 1481 1138 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3342 3342
Size: 6154
PshAI GACnn'nnGTC
Cuts at: 1613 2309 1613
Size: 696 5458
PsiI TTA'TAA
Cuts at: 3724 3724
Size: 6154
PspOMI G'GGCC_C
Cuts at: 2862 2862
Size: 6154
PspXI vC'TCGA_Gb
Cuts at: 2191 2191
Size: 6154
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 6122
PstI C_TGCA'G
Cuts at: 830 2490 3506 830
Size: 1660 1016 3478
Fragments arranged by size:
3478 1660 1016
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
PvuII CAG'CTG
Cuts at: 2921 2989 3241 2921
Size: 68 252 5834
Fragments arranged by size:
5834 252 68
SacI G_AGCT'C
Cuts at: 2198 2874 2943 2198
Size: 676 69 5409
Fragments arranged by size:
5409 676 69
SalI G'TCGA_C
Cuts at: 1936 3418 3496 1936
Size: 1482 78 4594
Fragments arranged by size:
4594 1482 78
SapI GCTCTTCn'nnn_
Cuts at: 2693 2693
Size: 6154
SbfI CC_TGCA'GG
Cuts at: 3506 3506
Size: 6154
ScaI AGT'ACT
Cuts at: 4402 6114 4402
Size: 1712 4442
SfcI C'TryA_G
Cuts at: 826 1045 2486 3502 4637 5318 5509 826
Size: 219 1441 1016 1135 681 191 1471
Fragments arranged by size:
1471 1441 1135 1016 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 2141 1700
Size: 441 5713
SmaI CCC'GGG
Cuts at: 1872 2272 3026 3481 1872
Size: 400 754 455 4545
Fragments arranged by size:
4545 754 455 400
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2191 2780 3172 3337 4258 5159
Size: 197 926 248 589 392 165 921 901
Cuts at: 5159 5421 820
Size: 262 1553
Fragments arranged by size:
1553 926 921 901 589 392 262 248
197 165
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 2434 152
Size: 2282 3872
SphI G_CATG'C
Cuts at: 1132 3512 1132
Size: 2380 3774
SspI AAT'ATT
Cuts at: 5 52 2518 4078 5
Size: 47 2466 1560 2081
Fragments arranged by size:
2466 2081 1560 47
StuI AGG'CCT
Cuts at: 1517 3194 1517
Size: 1677 4477
StyI C'CwwG_G
Cuts at: 513 1065 2169 2329 2634 3593 513
Size: 552 1104 160 305 959 3074
Fragments arranged by size:
3074 1104 959 552 305 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2162 3437 4181 4198 4351 4536 4981
Size: 1255 582 1275 744 17 153 185 445
Cuts at: 4981 325
Size: 1498
Fragments arranged by size:
1498 1275 1255 744 582 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2673 2929 3159
Size: 274 80 33 51 1443 696 256 230
Cuts at: 3159 4400 6112 96
Size: 1241 1712 138
Fragments arranged by size:
1712 1443 1241 696 274 256 230 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 6114 6146 3326
Size: 32 2756 32 3334
Fragments arranged by size:
3334 2756 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6154
XbaI T'CTAG_A
Cuts at: 3490 3490
Size: 6154
XhoI C'TCGA_G
Cuts at: 1943 2191 1943
Size: 248 5906
XmaI C'CCGG_G
Cuts at: 1870 2270 3024 3479 1870
Size: 400 754 455 4545
Fragments arranged by size:
4545 754 455 400
XmnI GAAnn'nnTTC
Cuts at: 4283 4283
Size: 6154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3962 276
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI