Restriction map for
pFN21AE0951
(Circular) (Six-base) MAPSORT of: pFN21AE0951.seq Check: 7293 from: 1 to: 6124
pFN21AE0951 6124 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 1937
Size: 1530 4594
Acc65I G'GTAC_C
Cuts at: 3445 3445
Size: 6124
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3255 3347 4187 5571 5839 1632
Size: 1623 92 840 1384 268 1917
Fragments arranged by size:
1917 1623 1384 840 268 92
AflII C'TTAA_G
Cuts at: 820 1017 2981 820
Size: 197 1964 3963
Fragments arranged by size:
3963 1964 197
AflIII A'CryG_T
Cuts at: 1203 2673 5023 6000 1203
Size: 1470 2350 977 1327
Fragments arranged by size:
2350 1470 1327 977
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 2677 4853 2677
Size: 2176 3948
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6124
AlwNI CAG_nnn'CTG
Cuts at: 3867 5439 3867
Size: 1572 4552
ApaI G_GGCC'C
Cuts at: 3154 3154
Size: 6124
ApaLI G'TGCA_C
Cuts at: 4181 5337 4181
Size: 1156 4968
ApoI r'AATT_y
Cuts at: 1493 2637 2763 2960 3135 3433 3659 3811 5920
Size: 1144 126 197 175 298 226 152 2109
Cuts at: 5920 1493
Size: 1697
Fragments arranged by size:
2109 1697 1144 298 226 197 175 152
126
AseI AT'TA_AT
Cuts at: 160 1032 2843 4678 160
Size: 872 1811 1835 1606
Fragments arranged by size:
1835 1811 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 3449 1870
Size: 73 128 1378 4545
Fragments arranged by size:
4545 1378 128 73
BamHI G'GATC_C
Cuts at: 2854 3454 3821 2854
Size: 600 367 5157
Fragments arranged by size:
5157 600 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 3253 3445 4900 618
Size: 325 217 627 233 1233 192 1455 1842
Fragments arranged by size:
1842 1455 1233 627 325 233 217 192
BanII G_rGCy'C
Cuts at: 1391 3154 3443 1391
Size: 1763 289 4072
Fragments arranged by size:
4072 1763 289
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2606 3335 928
Size: 971 707 729 3717
Fragments arranged by size:
3717 971 729 707
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4315 4349 1903
Size: 34 50 34 2294 34 3678
Fragments arranged by size:
3678 2294 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2929 4016 5226 2929
Size: 1087 1210 3827
Fragments arranged by size:
3827 1210 1087
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 4735 136
Size: 107 122 71 631 605 394 2669 1525
Fragments arranged by size:
2669 1525 631 605 394 122 107 71
BglII A'GATC_T
Cuts at: 6119 6119
Size: 6124
BlpI GC'TnA_GC
Cuts at: 2129 3180 3541 2129
Size: 1051 361 4712
Fragments arranged by size:
4712 1051 361
Bme1580I G_kGCm'C
Cuts at: 1790 2025 3154 3175 3258 4185 5341 1790
Size: 235 1129 21 83 927 1156 2573
Fragments arranged by size:
2573 1156 1129 927 235 83 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3855 4813 442
Size: 416 53 83 399 24 2438 958 1753
Fragments arranged by size:
2438 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3072 1056
Size: 2016 4108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2471 2511 2873 4784 1239
Size: 1232 40 362 1911 2579
Fragments arranged by size:
2579 1911 1232 362 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4249 5114 5412 4249
Size: 865 298 4961
Fragments arranged by size:
4961 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3069 4787 882
Size: 617 1570 1718 2219
Fragments arranged by size:
2219 1718 1570 617
BsaAI yAC'GTr
Cuts at: 493 1204 3915 493
Size: 711 2711 2702
Fragments arranged by size:
2711 2702 711
BsaBI GATnn'nnATC
Cuts at: 6118 6118
Size: 6124
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3931 4313 4948 275
Size: 53 83 186 936 2398 382 635 1451
Fragments arranged by size:
2398 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4557 4955 5229 5376 5787 4557
Size: 398 274 147 411 4894
Fragments arranged by size:
4894 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2774 2804 1178
Size: 30 165 30 1371 30 4498
Fragments arranged by size:
4498 1371 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2388 3317 3332 3353 1186
Size: 1202 929 15 21 3957
Fragments arranged by size:
3957 1202 929 21 15
BseYI C'CCAG_C
Cuts at: 2158 5327 5823 2158
Size: 3169 496 2459
Fragments arranged by size:
3169 2459 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3240 1163
Size: 573 1504 4047
Fragments arranged by size:
4047 1504 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3574 4335 5363 1540
Size: 275 186 1573 761 1028 2301
Fragments arranged by size:
2301 1573 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2595 4185 4270 5341 2595
Size: 1590 85 1071 3378
Fragments arranged by size:
3378 1590 1071 85
BsmI GAATG_Cn'
Cuts at: 3635 3728 3635
Size: 93 6031
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2595 3154 3175 3258 3443 4185
Size: 399 235 570 559 21 83 185 742
Cuts at: 4185 4270 5341 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 559 399 235 185
85 83 21
BspEI T'CCGG_A
Cuts at: 5787 5787
Size: 6124
BspHI T'CATG_A
Cuts at: 2595 4013 5743 2595
Size: 1418 1730 2976
Fragments arranged by size:
2976 1730 1418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3479 844
Size: 2635 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2942 4011 1394
Size: 429 1119 1069 3507
Fragments arranged by size:
3507 1119 1069 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4619 4793 65
Size: 1183 3371 174 1396
Fragments arranged by size:
3371 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4768 4955 1700
Size: 110 2958 187 2869
Fragments arranged by size:
2958 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2184 2926 96
Size: 2088 742 3294
Fragments arranged by size:
3294 2088 742
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 4184 5196 4184
Size: 1012 5112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6124
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 2053 1184
Size: 869 5255
BstXI CCAn_nnnn'nTGG
Cuts at: 2830 3265 2830
Size: 435 5689
BstYI r'GATC_y
Cuts at: 1972 2854 3454 3821 4206 4223 5675 5761 5773
Size: 882 600 367 385 17 1452 86 12
Cuts at: 5773 6119 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 882 600 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2664 2664
Size: 6124
BtgI C'CryG_G
Cuts at: 513 1065 2897 3040 513
Size: 552 1832 143 3597
Fragments arranged by size:
3597 1832 552 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3159 487
Size: 683 56 1933 3452
Fragments arranged by size:
3452 1933 683 56
BtsI GCAGTG_nn'
Cuts at: 3270 3640 4434 4454 3270
Size: 370 794 20 4940
Fragments arranged by size:
4940 794 370 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 3428 3428
Size: 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3571 4460 6089
Size: 54 1425 50 275 18 1741 889 1629
Cuts at: 6089 8
Size: 43
Fragments arranged by size:
1741 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2053 3016 3231 4054 1353
Size: 700 963 215 823 3423
Fragments arranged by size:
3423 963 823 700 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2023 4711 5085 5231 387
Size: 1064 138 434 2688 374 146 1280
Fragments arranged by size:
2688 1280 1064 434 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2471 2511 2873 3255 3347 4187 4784
Size: 393 839 40 362 382 92 840 597
Cuts at: 4784 5571 5839 1239
Size: 787 268 1524
Fragments arranged by size:
1524 840 839 787 597 393 382 362
268 92 40
EcoNI CCTnn'n_nnAGG
Cuts at: 3314 3314
Size: 6124
EcoO109I rG'GnC_Cy
Cuts at: 2205 3150 3241 3307 2205
Size: 945 91 66 5022
Fragments arranged by size:
5022 945 91 66
EcoRI G'AATT_C
Cuts at: 2763 2960 3433 3811 2763
Size: 197 473 378 5076
Fragments arranged by size:
5076 473 378 197
FspI TGC'GCA
Cuts at: 2629 4630 2629
Size: 2001 4123
HaeII r_GCGC'y
Cuts at: 1678 5271 1678
Size: 3593 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2473 2505 2663
Size: 32 135 32 563 32 306 32 158
Cuts at: 2663 2695 2795 2827 4765 4797 4839 4871 1373
Size: 32 100 32 1938 32 42 32 2626
Fragments arranged by size:
2626 1938 563 306 158 135 100 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3468 3714 669
Size: 988 281 1530 246 3079
Fragments arranged by size:
3079 1530 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2978 748
Size: 2230 3894
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KpnI G_GTAC'C
Cuts at: 3449 3449
Size: 6124
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2133 2139 5238 5422 1305
Size: 828 6 3099 184 2007
Fragments arranged by size:
3099 2007 828 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4302
Fragments arranged by size:
4302 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2475 4082 4441 4600 518
Size: 648 1309 1607 359 159 2042
Fragments arranged by size:
2042 1607 1309 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2990 3540 4217 5365 5610 1606
Size: 1384 550 677 1148 245 2120
Fragments arranged by size:
2120 1384 1148 677 550 245
NcoI C'CATG_G
Cuts at: 513 1065 3040 513
Size: 552 1975 3597
Fragments arranged by size:
3597 1975 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6124
NheI G'CTAG_C
Cuts at: 1052 3068 1052
Size: 2016 4108
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 2677 3482 5027 1132
Size: 97 1448 805 1545 2229
Fragments arranged by size:
2229 1545 1448 805 97
PasI CC'CwG_GG
Cuts at: 1348 2618 1348
Size: 1270 4854
PciI A'CATG_T
Cuts at: 2673 5023 2673
Size: 2350 3774
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6124
PfoI T'CCnGG_A
Cuts at: 2091 2448 3051 2091
Size: 357 603 5164
Fragments arranged by size:
5164 603 357
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 5706 5738 4193
Size: 32 1481 32 4579
Fragments arranged by size:
4579 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2205 2205
Size: 6124
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 2309 3694 2309
Size: 1385 4739
PspOMI G'GGCC_C
Cuts at: 3150 3150
Size: 6124
PstI C_TGCA'G
Cuts at: 830 2431 3476 830
Size: 1601 1045 3478
Fragments arranged by size:
3478 1601 1045
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SanDI GG'GwC_CC
Cuts at: 2205 2205
Size: 6124
SapI GCTCTTCn'nnn_
Cuts at: 2053 2053
Size: 6124
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 4372 6084 4372
Size: 1712 4412
SfcI C'TryA_G
Cuts at: 826 1045 2337 2427 2682 3472 4607 5288 5479
Size: 219 1292 90 255 790 1135 681 191
Cuts at: 5479 826
Size: 1471
Fragments arranged by size:
1471 1292 1135 790 681 255 219 191
90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 2073 3451 1872
Size: 201 1378 4545
Fragments arranged by size:
4545 1378 201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2981 4228 5129 5391 820
Size: 197 926 1038 1247 901 262 1553
Fragments arranged by size:
1553 1247 1038 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 3482 1132
Size: 2350 3774
SspI AAT'ATT
Cuts at: 5 52 2780 4048 5
Size: 47 2728 1268 2081
Fragments arranged by size:
2728 2081 1268 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6124
StyI C'CwwG_G
Cuts at: 513 1065 2823 3040 3258 3563 513
Size: 552 1758 217 218 305 3074
Fragments arranged by size:
3074 1758 552 305 218 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2041 4151 4168 4321 4506 4951 325
Size: 1255 461 2110 17 153 185 445 1498
Fragments arranged by size:
2110 1498 1255 461 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2926 4370
Size: 274 80 33 51 1443 207 742 1444
Cuts at: 4370 6082 96
Size: 1712 138
Fragments arranged by size:
1712 1444 1443 742 274 207 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2652 2684 6084 6116 2652
Size: 32 3400 32 2660
Fragments arranged by size:
3400 2660 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2831 1316
Size: 1515 4609
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 2071 3449 1870
Size: 201 1378 4545
Fragments arranged by size:
4545 1378 201
XmnI GAAnn'nnTTC
Cuts at: 4253 4253
Size: 6124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI