Restriction map for
pFN21AE0949
(Circular) (Six-base) MAPSORT of: pFN21AE0949.seq Check: 9472 from: 1 to: 5998
pFN21AE0949 5998 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 3341 1937
Size: 694 710 4594
Fragments arranged by size:
4594 710 694
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 3068 4061 5445 5713 1632
Size: 932 504 993 1384 268 1917
Fragments arranged by size:
1917 1384 993 932 504 268
AflII C'TTAA_G
Cuts at: 820 1017 2074 820
Size: 197 1057 4744
Fragments arranged by size:
4744 1057 197
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 4727 4727
Size: 5998
AleI CACnn'nnGTG
Cuts at: 1166 2586 1166
Size: 1420 4578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5998
AlwNI CAG_nnn'CTG
Cuts at: 2866 3741 5313 2866
Size: 875 1572 3551
Fragments arranged by size:
3551 1572 875
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 3307 3533 3685 5794 1493
Size: 1814 226 152 2109 1697
Fragments arranged by size:
2109 1814 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 2741 3323 1870
Size: 73 798 582 4545
Fragments arranged by size:
4545 798 582 73
BamHI G'GATC_C
Cuts at: 3328 3695 3328
Size: 367 5631
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3319 4774 618
Size: 325 217 627 1532 1455 1842
Fragments arranged by size:
1842 1532 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2144 2945 3317 1391
Size: 753 801 372 4072
Fragments arranged by size:
4072 801 753 372
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5027
BbvCI CC'TCA_GC
Cuts at: 2657 2925 2657
Size: 268 5730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4189 4223 1903
Size: 34 2252 34 3678
Fragments arranged by size:
3678 2252 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2899 3890 5100 2899
Size: 991 1210 3797
Fragments arranged by size:
3797 1210 991
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3167 4609 136
Size: 107 122 71 631 605 1495 1442 1525
Fragments arranged by size:
1525 1495 1442 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2473 5993 2473
Size: 3520 2478
BlpI GC'TnA_GC
Cuts at: 2735 3415 2735
Size: 680 5318
Bme1580I G_kGCm'C
Cuts at: 1790 2895 4059 5215 1790
Size: 1105 1164 1156 2573
Fragments arranged by size:
2573 1164 1156 1105
BmgBI CAC'GTC
Cuts at: 1318 3158 1318
Size: 1840 4158
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3102 3729 4687
Size: 416 53 83 399 24 1685 627 958
Cuts at: 4687 442
Size: 1753
Fragments arranged by size:
1753 1685 958 627 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2735 2747 3296 4658 1239
Size: 1496 12 549 1362 2579
Fragments arranged by size:
2579 1496 1362 549 12
Bpu10I CC'TnA_GC
Cuts at: 2657 2925 2657
Size: 268 5730
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4123 4988 5286 4123
Size: 865 298 4835
Fragments arranged by size:
4835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2053 4661 882
Size: 617 554 2608 2219
Fragments arranged by size:
2608 2219 617 554
BsaAI yAC'GTr
Cuts at: 493 1204 2293 3789 493
Size: 711 1089 1496 2702
Fragments arranged by size:
2702 1496 1089 711
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3805 4187 4822 275
Size: 53 83 186 936 2272 382 635 1451
Fragments arranged by size:
2272 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4431 4829 5103 5250 5661 4431
Size: 398 274 147 411 4768
Fragments arranged by size:
4768 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2277 2307 3008 3038 1178
Size: 30 165 30 874 30 701 30 4138
Fragments arranged by size:
4138 874 701 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2942 1186
Size: 1756 4242
BseYI C'CCAG_C
Cuts at: 5201 5697 5201
Size: 496 5502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5425
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2144 4059 4144 5215 2144
Size: 1915 85 1071 2927
Fragments arranged by size:
2927 1915 1071 85
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2895 2945 3317 4059 4144 5215
Size: 399 354 751 50 372 742 85 1071
Cuts at: 5215 1391
Size: 2174
Fragments arranged by size:
2174 1071 751 742 399 372 354 85
50
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 2178 3887 5617 2178
Size: 1709 1730 2559
Fragments arranged by size:
2559 1730 1709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3353 844
Size: 2509 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3885 1394
Size: 429 2062 3507
Fragments arranged by size:
3507 2062 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4493 4667 65
Size: 1183 3245 174 1396
Fragments arranged by size:
3245 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4642 4829 1700
Size: 110 2832 187 2869
Fragments arranged by size:
2869 2832 187 110
BsrGI T'GTAC_A
Cuts at: 96 2483 2578 96
Size: 2387 95 3516
Fragments arranged by size:
3516 2387 95
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 4058 5070 4058
Size: 1012 4986
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2539 794
Size: 1745 4253
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstXI CCAn_nnnn'nTGG
Cuts at: 2335 2335
Size: 5998
BstYI r'GATC_y
Cuts at: 1972 2473 3328 3695 4080 4097 5549 5635 5647
Size: 501 855 367 385 17 1452 86 12
Cuts at: 5647 5993 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 855 501 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2671 2729 513
Size: 552 1606 58 3782
Fragments arranged by size:
3782 1606 552 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5259
Fragments arranged by size:
5259 683 56
BtsI GCAGTG_nn'
Cuts at: 3083 3514 4308 4328 3083
Size: 431 794 20 4753
Fragments arranged by size:
4753 794 431 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2398 2433 529
Size: 35 1834 35 4094
Fragments arranged by size:
4094 1834 35 35
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DraIII CAC_nnn'GTG
Cuts at: 3070 3070
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2820 2827 2958
Size: 54 1425 50 275 18 990 7 131
Cuts at: 2958 3445 4334 5963 8
Size: 487 889 1629 43
Fragments arranged by size:
1629 1425 990 889 487 275 131 54
50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2381 3264 3928 1353
Size: 1028 883 664 3423
Fragments arranged by size:
3423 1028 883 664
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4585 4959 5105 387
Size: 1064 138 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2142 2142
Size: 5998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2564 2735 2747 3068 3296 4061 4658
Size: 393 932 171 12 321 228 765 597
Cuts at: 4658 5445 5713 1239
Size: 787 268 1524
Fragments arranged by size:
1524 932 787 765 597 393 321 268
228 171 12
EcoO109I rG'GnC_Cy
Cuts at: 2245 2548 2746 2245
Size: 303 198 5497
Fragments arranged by size:
5497 303 198
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
FspI TGC'GCA
Cuts at: 2648 4504 2648
Size: 1856 4142
HaeII r_GCGC'y
Cuts at: 1678 5145 1678
Size: 3467 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2158 2245 2277 2765
Size: 32 135 32 554 32 87 32 488
Cuts at: 2765 2797 3008 3040 4639 4671 4713 4745 1373
Size: 32 211 32 1599 32 42 32 2626
Fragments arranged by size:
2626 1599 554 488 211 135 87 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2319 748
Size: 1571 4427
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2883 3033 5112 5296 1305
Size: 1578 150 2079 184 2007
Fragments arranged by size:
2079 2007 1578 184 150
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2822 2960 10
Size: 54 1425 343 990 138 3048
Fragments arranged by size:
3048 1425 990 343 138 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2586 2861 3956 4315 4474 518
Size: 648 1420 275 1095 359 159 2042
Fragments arranged by size:
2042 1420 1095 648 359 275 159
MspA1I CmG'CkG
Cuts at: 1606 2680 2924 3414 4091 5239 5484 1606
Size: 1074 244 490 677 1148 245 2120
Fragments arranged by size:
2120 1148 1074 677 490 245 244
NcoI C'CATG_G
Cuts at: 513 1065 2671 513
Size: 552 1606 3840
Fragments arranged by size:
3840 1606 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 2366 3356 4901 1132
Size: 97 1137 990 1545 2229
Fragments arranged by size:
2229 1545 1137 990 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5998
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2569 3070 1108
Size: 1461 501 4036
Fragments arranged by size:
4036 1461 501
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2245 2746 2245
Size: 501 5497
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PstI C_TGCA'G
Cuts at: 830 2535 3350 830
Size: 1705 815 3478
Fragments arranged by size:
3478 1705 815
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
SacI G_AGCT'C
Cuts at: 2144 2144
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SapI GCTCTTCn'nnn_
Cuts at: 3264 3264
Size: 5998
SbfI CC_TGCA'GG
Cuts at: 2535 3350 2535
Size: 815 5183
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SfcI C'TryA_G
Cuts at: 826 1045 2531 2791 3346 4481 5162 5353 826
Size: 219 1486 260 555 1135 681 191 1471
Fragments arranged by size:
1486 1471 1135 681 555 260 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 3325 1872
Size: 1453 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2074 4102 5003 5265 820
Size: 197 926 131 2028 901 262 1553
Fragments arranged by size:
2028 1553 926 901 262 197 131
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 2671 3437 513
Size: 552 1606 766 3074
Fragments arranged by size:
3074 1606 766 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2873 4025 4042 4195 4380 4825 325
Size: 1255 1293 1152 17 153 185 445 1498
Fragments arranged by size:
1498 1293 1255 1152 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2483 2578 2709
Size: 274 80 33 51 1443 506 95 131
Cuts at: 2709 4244 5956 96
Size: 1535 1712 138
Fragments arranged by size:
1712 1535 1443 506 274 138 131 95
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2277 2309 5958 5990 2277
Size: 32 3649 32 2285
Fragments arranged by size:
3649 2285 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 3323 1870
Size: 1453 4545
XmnI GAAnn'nnTTC
Cuts at: 3183 4127 3183
Size: 944 5054
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI