Restriction map for
pFN21AE0944
(Circular) (Six-base) MAPSORT of: pFN21AE0944.seq Check: 7985 from: 1 to: 5734
pFN21AE0944 5734 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2441 2441
Size: 5734
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3000 3544 278
Size: 53 83 186 936 1464 544 2468
Fragments arranged by size:
2468 1464 936 544 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2021 3077 1937
Size: 84 1056 4594
Fragments arranged by size:
4594 1056 84
Acc65I G'GTAC_C
Cuts at: 2757 3055 2757
Size: 298 5436
AclI AA'CG_TT
Cuts at: 1563 3033 3861 4234 1563
Size: 1470 828 373 3063
Fragments arranged by size:
3063 1470 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2776 3797 5181 5449 1632
Size: 1144 1021 1384 268 1917
Fragments arranged by size:
1917 1384 1144 1021 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 2712 4633 5610 1203
Size: 1509 1921 977 1327
Fragments arranged by size:
1921 1509 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5734
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2120 2152 2120
Size: 32 5702
AlwNI CAG_nnn'CTG
Cuts at: 3477 5049 3477
Size: 1572 4162
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 2543 2907 2950 3043 3269 3421 5530 1493
Size: 1050 364 43 93 226 152 2109 1697
Fragments arranged by size:
2109 1697 1050 364 226 152 93 43
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2140 2150 2571 2695 3059 1870
Size: 73 197 10 421 124 364 4545
Fragments arranged by size:
4545 421 364 197 124 73 10
BamHI G'GATC_C
Cuts at: 3064 3431 3064
Size: 367 5367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2308 2757 2989 3055 4510
Size: 325 217 627 521 449 232 66 1455
Cuts at: 4510 618
Size: 1842
Fragments arranged by size:
1842 1455 627 521 449 325 232 217
66
BanII G_rGCy'C
Cuts at: 1391 2109 2147 2883 3053 1391
Size: 718 38 736 170 4072
Fragments arranged by size:
4072 736 718 170 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2601 928
Size: 971 702 4061
Fragments arranged by size:
4061 971 702
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3925 3959 1903
Size: 34 1988 34 3678
Fragments arranged by size:
3678 1988 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3626 4836 3626
Size: 1210 4524
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BfrBI ATG'CAT
Cuts at: 2416 2416
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4345 136
Size: 107 122 71 631 605 2673 1525
Fragments arranged by size:
2673 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2670 5729 2670
Size: 3059 2675
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 3795 4951 1790
Size: 2005 1156 2573
Fragments arranged by size:
2573 2005 1156
BmgBI CAC'GTC
Cuts at: 1318 2713 1318
Size: 1395 4339
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3465 4423 442
Size: 416 53 83 399 24 2048 958 1753
Fragments arranged by size:
2048 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 4394 1239
Size: 995 2160 2579
Fragments arranged by size:
2579 2160 995
Bpu10I CC'TnA_GC
Cuts at: 2967 2967
Size: 5734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2516 3859 4724 5022 2516
Size: 1343 865 298 3228
Fragments arranged by size:
3228 1343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2214 2366 4397 882
Size: 617 715 152 2031 2219
Fragments arranged by size:
2219 2031 715 617 152
BsaAI yAC'GTr
Cuts at: 493 1204 2659 3525 493
Size: 711 1455 866 2702
Fragments arranged by size:
2702 1455 866 711
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2598 2997 3541 3923
Size: 53 83 186 936 1065 399 544 382
Cuts at: 3923 4558 275
Size: 635 1451
Fragments arranged by size:
1451 1065 936 635 544 399 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4167 4565 4839 4986 5397 4167
Size: 398 274 147 411 4504
Fragments arranged by size:
4504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 2211 2241 2252
Size: 30 165 30 717 30 61 30 11
Cuts at: 2252 2282 1178
Size: 30 4630
Fragments arranged by size:
4630 717 165 61 30 30 30 30
30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2273 2465 2468 1186
Size: 1087 192 3 4452
Fragments arranged by size:
4452 1087 192 3
BseYI C'CCAG_C
Cuts at: 2108 2780 4937 5433 2108
Size: 672 2157 496 2409
Fragments arranged by size:
2409 2157 672 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2231 1163
Size: 573 495 4666
Fragments arranged by size:
4666 573 495
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3184 3945 4973 1540
Size: 275 186 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3795 3880 4951 3795
Size: 85 1071 4578
Fragments arranged by size:
4578 1071 85
BsmI GAATG_Cn'
Cuts at: 2416 2865 3245 3338 2416
Size: 449 380 93 4812
Fragments arranged by size:
4812 449 380 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2950 2950
Size: 5734
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2147 2883 3053 3795 3880 4951
Size: 399 319 38 736 170 742 85 1071
Cuts at: 4951 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 736 399 319 170 85
38
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 3623 5353 3623
Size: 1730 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2441 3089 844
Size: 1597 648 3489
Fragments arranged by size:
3489 1597 648
BsrBI CCG'CTC
Cuts at: 1394 1823 2890 3621 1394
Size: 429 1067 731 3507
Fragments arranged by size:
3507 1067 731 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2170 4229 4403 65
Size: 1183 922 2059 174 1396
Fragments arranged by size:
2059 1396 1183 922 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4378 4565 1700
Size: 110 2568 187 2869
Fragments arranged by size:
2869 2568 187 110
BsrGI T'GTAC_A
Cuts at: 96 2736 96
Size: 2640 3094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2963 794
Size: 2169 3565
BstBI TT'CG_AA
Cuts at: 2076 4538 2076
Size: 2462 3272
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstXI CCAn_nnnn'nTGG
Cuts at: 2749 2749
Size: 5734
BstYI r'GATC_y
Cuts at: 1972 2670 2799 3064 3431 3816 3833 5285 5371
Size: 698 129 265 367 385 17 1452 86
Cuts at: 5371 5383 5729 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 698 385 367 346 265 129
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2100 513
Size: 552 1035 4147
Fragments arranged by size:
4147 1035 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4995
Fragments arranged by size:
4995 683 56
BtsI GCAGTG_nn'
Cuts at: 2496 2497 3250 4044 4064 2496
Size: 1 753 794 20 4166
Fragments arranged by size:
4166 794 753 20 1
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5699
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2809 3181 4070
Size: 54 1425 50 275 18 979 372 889
Cuts at: 4070 5699 8
Size: 1629 43
Fragments arranged by size:
1629 1425 979 889 372 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 2556 2795 3003 3664 1353
Size: 720 483 239 208 661 3423
Fragments arranged by size:
3423 720 661 483 239 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4321 4695 4841 387
Size: 1064 138 2732 374 146 1280
Fragments arranged by size:
2732 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2776 3797 4394 5181 5449 1239
Size: 393 602 542 1021 597 787 268 1524
Fragments arranged by size:
1524 1021 787 602 597 542 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2527 2527
Size: 5734
EcoRI G'AATT_C
Cuts at: 2950 3043 3421 2950
Size: 93 378 5263
Fragments arranged by size:
5263 378 93
EcoRV GAT'ATC
Cuts at: 2284 2284
Size: 5734
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 4881 1678
Size: 3203 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 2615 2647 2819
Size: 32 135 32 500 32 511 32 172
Cuts at: 2819 2851 4375 4407 4449 4481 1373
Size: 32 1524 32 42 32 2626
Fragments arranged by size:
2626 1524 511 500 172 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3001 3078 3324 669
Size: 988 281 1063 77 246 3079
Fragments arranged by size:
3079 1063 988 281 246 77
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 2761 3059 2761
Size: 298 5436
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2184 2658 4848 5032 1305
Size: 879 474 2190 184 2007
Fragments arranged by size:
2190 2007 879 474 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2811 10
Size: 54 1425 343 979 2933
Fragments arranged by size:
2933 1425 979 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2502 2747 3692 4051 4210 518
Size: 648 1336 245 945 359 159 2042
Fragments arranged by size:
2042 1336 945 648 359 245 159
MspA1I CmG'CkG
Cuts at: 1606 2102 2253 2289 2986 3150 3827 4975 5220
Size: 496 151 36 697 164 677 1148 245
Cuts at: 5220 1606
Size: 2120
Fragments arranged by size:
2120 1148 697 677 496 245 164 151
36
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5182
NdeI CA'TA_TG
Cuts at: 387 2959 387
Size: 2572 3162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NsiI A_TGCA'T
Cuts at: 2418 2418
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 2416 3092 4637 1132
Size: 97 1187 676 1545 2229
Fragments arranged by size:
2229 1545 1187 676 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5734
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5734
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2120 2152 3803 3835 5316 5348 2120
Size: 32 1651 32 1481 32 2506
Fragments arranged by size:
2506 1651 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 2527
Size: 5734
PshAI GACnn'nnGTC
Cuts at: 1613 2573 1613
Size: 960 4774
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PstI C_TGCA'G
Cuts at: 830 3086 830
Size: 2256 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
PvuII CAG'CTG
Cuts at: 2289 2986 2289
Size: 697 5037
SacII CC_GC'GG
Cuts at: 2103 2103
Size: 5734
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 2931 3082 4217 4898 5089 826
Size: 219 1886 151 1135 681 191 1471
Fragments arranged by size:
1886 1471 1135 681 219 191 151
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 3061 1872
Size: 1189 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2531 3838 4739 5001 820
Size: 197 926 588 1307 901 262 1553
Fragments arranged by size:
1553 1307 926 901 588 262 197
SnaBI TAC'GTA
Cuts at: 493 2659 3525 493
Size: 2166 866 2702
Fragments arranged by size:
2702 2166 866
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5734
StyI C'CwwG_G
Cuts at: 513 1065 2841 2921 3173 513
Size: 552 1776 80 252 3074
Fragments arranged by size:
3074 1776 552 252 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 3761 3778 3931 4116 4561 325
Size: 1255 777 1404 17 153 185 445 1498
Fragments arranged by size:
1498 1404 1255 777 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2736 3980 5692
Size: 274 80 33 51 1443 759 1244 1712
Cuts at: 5692 96
Size: 138
Fragments arranged by size:
1712 1443 1244 759 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 5694 5726 2252
Size: 32 3410 32 2260
Fragments arranged by size:
3410 2260 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2833 1316
Size: 1517 4217
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 3059 1870
Size: 1189 4545
XmnI GAAnn'nnTTC
Cuts at: 3863 3863
Size: 5734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2998 3542 276
Size: 53 83 186 936 1464 544 2468
Fragments arranged by size:
2468 1464 936 544 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI