Restriction map for
pFN21AE0937
(Circular) (Six-base) MAPSORT of: pFN21AE0937.seq Check: 6924 from: 1 to: 5989
pFN21AE0937 5989 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2250 3332 1937
Size: 313 1082 4594
Fragments arranged by size:
4594 1082 313
Acc65I G'GTAC_C
Cuts at: 3310 3310
Size: 5989
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2655 4052 5436 5704 1632
Size: 1023 1397 1384 268 1917
Fragments arranged by size:
1917 1397 1384 1023 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 4888 5865 1203
Size: 3685 977 1327
Fragments arranged by size:
3685 1327 977
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5989
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5989
AlwNI CAG_nnn'CTG
Cuts at: 2035 2047 2503 2561 3732 5304 2035
Size: 12 456 58 1171 1572 2720
Fragments arranged by size:
2720 1572 1171 456 58 12
ApaI G_GGCC'C
Cuts at: 2763 3279 2763
Size: 516 5473
ApaLI G'TGCA_C
Cuts at: 3218 4046 5202 3218
Size: 828 1156 4005
Fragments arranged by size:
4005 1156 828
ApoI r'AATT_y
Cuts at: 1493 2102 3298 3524 3676 5785 1493
Size: 609 1196 226 152 2109 1697
Fragments arranged by size:
2109 1697 1196 609 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4543 160
Size: 872 3511 1606
Fragments arranged by size:
3511 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 2470 2584 2675 2952 2977 3314 1870
Size: 73 527 114 91 277 25 337 4545
Fragments arranged by size:
4545 527 337 277 114 91 73 25
BamHI G'GATC_C
Cuts at: 3319 3686 3319
Size: 367 5622
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3310 4765 618
Size: 325 217 627 1523 1455 1842
Fragments arranged by size:
1842 1523 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2763 3279 3308 1391
Size: 1372 516 29 4072
Fragments arranged by size:
4072 1372 516 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5018
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4180 4214 1903
Size: 34 2243 34 3678
Fragments arranged by size:
3678 2243 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3881 5091 3881
Size: 1210 4779
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2857 4600 136
Size: 107 122 71 631 605 1185 1743 1525
Fragments arranged by size:
1743 1525 1185 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2922 5984 2922
Size: 3062 2927
BlpI GC'TnA_GC
Cuts at: 2183 3406 2183
Size: 1223 4766
Bme1580I G_kGCm'C
Cuts at: 1790 2763 3222 3279 4050 5206 1790
Size: 973 459 57 771 1156 2573
Fragments arranged by size:
2573 1156 973 771 459 57
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5989
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2544 3720 4678
Size: 416 53 83 399 24 1127 1176 958
Cuts at: 4678 442
Size: 1753
Fragments arranged by size:
1753 1176 1127 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2648 3023 3160 4649 1239
Size: 1409 375 137 1489 2579
Fragments arranged by size:
2579 1489 1409 375 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4114 4979 5277 4114
Size: 865 298 4826
Fragments arranged by size:
4826 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4652 882
Size: 617 3153 2219
Fragments arranged by size:
3153 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3780 493
Size: 711 2576 2702
Fragments arranged by size:
2702 2576 711
BsaBI GATnn'nnATC
Cuts at: 5983 5983
Size: 5989
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3796 4178 4813 275
Size: 53 83 186 936 2263 382 635 1451
Fragments arranged by size:
2263 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4422 4820 5094 5241 5652 4422
Size: 398 274 147 411 4759
Fragments arranged by size:
4759 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 2587 2617 2780
Size: 30 165 30 657 30 497 30 163
Cuts at: 2780 2810 1178
Size: 30 4357
Fragments arranged by size:
4357 657 497 165 163 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 1186
Size: 952 5037
BseYI C'CCAG_C
Cuts at: 2307 2928 3110 5192 5688 2307
Size: 621 182 2082 496 2608
Fragments arranged by size:
2608 2082 621 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2433 2574 1163
Size: 573 697 141 4578
Fragments arranged by size:
4578 697 573 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3439 4200 5228 1540
Size: 275 186 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2288 2547 2600 3146 3222 3267 4050 4135 5206
Size: 259 53 546 76 45 783 85 1071
Cuts at: 5206 2288
Size: 3071
Fragments arranged by size:
3071 1071 783 546 259 85 76 53
45
BsmI GAATG_Cn'
Cuts at: 3129 3500 3593 3129
Size: 371 93 5525
Fragments arranged by size:
5525 371 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2288 2547 2600 2763 3146 3222 3267
Size: 399 498 259 53 163 383 76 45
Cuts at: 3267 3279 3308 4050 4135 5206 1391
Size: 12 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 498 399 383 259 163
85 76 53 45 29 12
BspEI T'CCGG_A
Cuts at: 5652 5652
Size: 5989
BspHI T'CATG_A
Cuts at: 3878 5608 3878
Size: 1730 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2647 3344 844
Size: 1803 697 3489
Fragments arranged by size:
3489 1803 697
BsrBI CCG'CTC
Cuts at: 1394 1823 2235 3876 1394
Size: 429 412 1641 3507
Fragments arranged by size:
3507 1641 429 412
BsrDI GCAATG_nn'
Cuts at: 65 1248 2068 3156 4484 4658 65
Size: 1183 820 1088 1328 174 1396
Fragments arranged by size:
1396 1328 1183 1088 820 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2642 3052 4633 4820 1700
Size: 110 832 410 1581 187 2869
Fragments arranged by size:
2869 1581 832 410 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 4049 5061 4049
Size: 1012 4977
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2035 2561 794
Size: 1241 526 4222
Fragments arranged by size:
4222 1241 526
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5989
BstYI r'GATC_y
Cuts at: 1972 2922 3319 3686 4071 4088 5540 5626 5638
Size: 950 397 367 385 17 1452 86 12
Cuts at: 5638 5984 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 950 397 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2271 2271
Size: 5989
BtgI C'CryG_G
Cuts at: 513 1065 2004 2865 3258 513
Size: 552 939 861 393 3244
Fragments arranged by size:
3244 939 861 552 393
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5250
Fragments arranged by size:
5250 683 56
BtsI GCAGTG_nn'
Cuts at: 3505 4299 4319 3505
Size: 794 20 5175
Fragments arranged by size:
5175 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5954
DraI TTT'AAA
Cuts at: 3293 3293
Size: 5989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4996 809
Size: 507 3680 1802
Fragments arranged by size:
3680 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2065 2236 2899
Size: 54 1425 50 275 18 235 171 663
Cuts at: 2899 3174 3436 4325 5954 8
Size: 275 262 889 1629 43
Fragments arranged by size:
1629 1425 889 663 275 275 262 235
171 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2631 2694 2763 3919 1353
Size: 1278 63 69 1156 3423
Fragments arranged by size:
3423 1278 1156 69 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4576 4950 5096 387
Size: 1064 138 2987 374 146 1280
Fragments arranged by size:
2987 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2648 2655 3023 3160 4052 4649 5436
Size: 393 1016 7 368 137 892 597 787
Cuts at: 5436 5704 1239
Size: 268 1524
Fragments arranged by size:
1524 1016 892 787 597 393 368 268
137 7
EcoO109I rG'GnC_Cy
Cuts at: 2680 2759 2956 2999 3275 3276 2680
Size: 79 197 43 276 1 5393
Fragments arranged by size:
5393 276 197 79 43 1
EcoRI G'AATT_C
Cuts at: 2102 3298 3676 2102
Size: 1196 378 4415
Fragments arranged by size:
4415 1196 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3202 3202
Size: 5989
FspI TGC'GCA
Cuts at: 4495 4495
Size: 5989
HaeII r_GCGC'y
Cuts at: 1678 2579 3059 3213 5136 1678
Size: 901 480 154 1923 2531
Fragments arranged by size:
2531 1923 901 480 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 2587 2619 3233
Size: 32 135 32 797 32 186 32 614
Cuts at: 3233 3265 4630 4662 4704 4736 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1365 797 614 186 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3333 3579 669
Size: 988 281 1395 246 3079
Fragments arranged by size:
3079 1395 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5989
HpaI GTT'AAC
Cuts at: 3579 3579
Size: 5989
KpnI G_GTAC'C
Cuts at: 3314 3314
Size: 5989
MfeI C'AATT_G
Cuts at: 3588 3588
Size: 5989
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2754 5103 5287 1305
Size: 1449 2349 184 2007
Fragments arranged by size:
2349 2007 1449 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2067 10
Size: 54 1425 343 235 3932
Fragments arranged by size:
3932 1425 343 235 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3947 4306 4465 518
Size: 648 2781 359 159 2042
Fragments arranged by size:
2781 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2867 3405 4082 5230 5475 1606
Size: 438 823 538 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 677 538 438 245
NaeI GCC'GGC
Cuts at: 2644 3054 2644
Size: 410 5579
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4498
Fragments arranged by size:
4498 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5989
NgoMIV G'CCGG_C
Cuts at: 2642 3052 2642
Size: 410 5579
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 2366 2467 3347 4892 1132
Size: 97 1137 101 880 1545 2229
Fragments arranged by size:
2229 1545 1137 880 101 97
PasI CC'CwG_GG
Cuts at: 1348 2372 3272 1348
Size: 1024 900 4065
Fragments arranged by size:
4065 1024 900
PciI A'CATG_T
Cuts at: 4888 4888
Size: 5989
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3203 1108
Size: 2095 3894
PfoI T'CCnGG_A
Cuts at: 2078 2078
Size: 5989
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2956 2999 2956
Size: 43 5946
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5989
PsiI TTA'TAA
Cuts at: 3559 3559
Size: 5989
PspOMI G'GGCC_C
Cuts at: 2759 3275 2759
Size: 516 5473
PstI C_TGCA'G
Cuts at: 830 2043 2508 2566 2658 3341 830
Size: 1213 465 58 92 683 3478
Fragments arranged by size:
3478 1213 683 465 92 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
PvuII CAG'CTG
Cuts at: 2044 2044
Size: 5989
SacII CC_GC'GG
Cuts at: 2868 2868
Size: 5989
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SanDI GG'GwC_CC
Cuts at: 2999 2999
Size: 5989
SapI GCTCTTCn'nnn_
Cuts at: 2694 2694
Size: 5989
SbfI CC_TGCA'GG
Cuts at: 2566 3341 2566
Size: 775 5214
ScaI AGT'ACT
Cuts at: 4237 5949 4237
Size: 1712 4277
SfcI C'TryA_G
Cuts at: 826 1045 2039 2504 2562 2654 3337 4472 5153
Size: 219 994 465 58 92 683 1135 681
Cuts at: 5153 5344 826
Size: 191 1471
Fragments arranged by size:
1471 1135 994 683 681 465 219 191
92 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 2954 3316 1872
Size: 1082 362 4545
Fragments arranged by size:
4545 1082 362
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4093 4994 5256 820
Size: 197 926 2150 901 262 1553
Fragments arranged by size:
2150 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5989
SphI G_CATG'C
Cuts at: 1132 3347 1132
Size: 2215 3774
SspI AAT'ATT
Cuts at: 5 52 3913 5
Size: 47 3861 2081
Fragments arranged by size:
3861 2081 47
StuI AGG'CCT
Cuts at: 1517 2621 1517
Size: 1104 4885
StyI C'CwwG_G
Cuts at: 513 1065 2004 2406 2613 2763 2814 3428 513
Size: 552 939 402 207 150 51 614 3074
Fragments arranged by size:
3074 939 614 552 402 207 150 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2419 3124 4016 4033 4186 4371 4816
Size: 1255 839 705 892 17 153 185 445
Cuts at: 4816 325
Size: 1498
Fragments arranged by size:
1498 1255 892 839 705 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2460 4235 5947
Size: 274 80 33 51 1443 483 1775 1712
Cuts at: 5947 96
Size: 138
Fragments arranged by size:
1775 1712 1443 483 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2060 2092 3136 3168 5949 5981 2060
Size: 32 1044 32 2781 32 2068
Fragments arranged by size:
2781 2068 1044 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2381 1316
Size: 1065 4924
XbaI T'CTAG_A
Cuts at: 3325 3325
Size: 5989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 2770 2341
Size: 429 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 2952 3314 1870
Size: 1082 362 4545
Fragments arranged by size:
4545 1082 362
XmnI GAAnn'nnTTC
Cuts at: 4118 4118
Size: 5989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI
SfiI SfoI SrfI SwaI