Restriction map for
pFN21AE0935
(Circular) (Six-base) MAPSORT of: pFN21AE0935.seq Check: 4206 from: 1 to: 5959
pFN21AE0935 5959 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2043 3302 1937
Size: 106 1259 4594
Fragments arranged by size:
4594 1259 106
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 4022 5406 5674 1632
Size: 662 1728 1384 268 1917
Fragments arranged by size:
1917 1728 1384 662 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 2754 2838 1166
Size: 1588 84 4287
Fragments arranged by size:
4287 1588 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 3702 5274 3702
Size: 1572 4387
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2283 2510 2661 2745 2829 2913 3081 3165
Size: 790 227 151 84 84 84 168 84
Cuts at: 3165 3247 3268 3494 3646 5755 1493
Size: 82 21 226 152 2109 1697
Fragments arranged by size:
2109 1697 790 227 226 168 152 151
84 84 84 84 82 21
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3280 4735 618
Size: 325 217 627 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2196 2975 3278 1391
Size: 805 779 303 4072
Fragments arranged by size:
4072 805 779 303
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 2080 1548
Size: 532 5427
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2446 5954 2446
Size: 3508 2451
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 4020 5176 1790
Size: 2230 1156 2573
Fragments arranged by size:
2573 2230 1156
BmgBI CAC'GTC
Cuts at: 1318 2336 1318
Size: 1018 4941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2299 2931 3099
Size: 416 53 83 399 24 882 632 168
Cuts at: 3099 3265 3690 4648 442
Size: 166 425 958 1753
Fragments arranged by size:
1753 958 882 632 425 416 399 168
166 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2335 4619 1239
Size: 1096 2284 2579
Fragments arranged by size:
2579 2284 1096
Bpu10I CC'TnA_GC
Cuts at: 2010 2010
Size: 5959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2623 4084 4949 5247 2623
Size: 1461 865 298 3335
Fragments arranged by size:
3335 1461 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2249 4622 882
Size: 617 750 2373 2219
Fragments arranged by size:
2373 2219 750 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3766 4148 4783 275
Size: 53 83 186 936 2233 382 635 1451
Fragments arranged by size:
2233 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2213 1178
Size: 30 165 30 780 30 4924
Fragments arranged by size:
4924 780 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 1186
Size: 886 5073
BseYI C'CCAG_C
Cuts at: 5162 5658 5162
Size: 496 5463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2351 2975 4020 4105 5176 2351
Size: 624 1045 85 1071 3134
Fragments arranged by size:
3134 1071 1045 624 85
BsmI GAATG_Cn'
Cuts at: 2072 2143 2861 2870 3470 3563 2072
Size: 71 718 9 600 93 4468
Fragments arranged by size:
4468 718 600 93 71 9
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2351 2975 3278 4020 4105 5176
Size: 399 406 155 624 303 742 85 1071
Cuts at: 5176 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 624 406 399 303 155
85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2440 3848 5578 2440
Size: 1408 1730 2821
Fragments arranged by size:
2821 1730 1408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3314 844
Size: 2470 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2962 4454 4628 65
Size: 1183 1714 1492 174 1396
Fragments arranged by size:
1714 1492 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2865 794
Size: 2071 3888
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2446 3289 3656 4041 4058 5510 5596 5608
Size: 474 843 367 385 17 1452 86 12
Cuts at: 5608 5954 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 843 474 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4468
Fragments arranged by size:
4468 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2231 3406 4295
Size: 54 1425 50 275 18 401 1175 889
Cuts at: 4295 5924 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1175 889 401 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3889 1353
Size: 2536 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2973 2973
Size: 5959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2335 4022 4619 5406 5674 1239
Size: 393 662 41 1687 597 787 268 1524
Fragments arranged by size:
1687 1524 787 662 597 393 268 41
EcoRI G'AATT_C
Cuts at: 2745 2829 2913 3081 3165 3268 3646 2745
Size: 84 84 168 84 103 378 5058
Fragments arranged by size:
5058 378 168 103 84 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 5959
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2452 2484 4600 4632 4674
Size: 32 135 32 880 32 2116 32 42
Cuts at: 4674 4706 1373
Size: 32 2626
Fragments arranged by size:
2626 2116 880 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 2426 3558 2426
Size: 1132 4827
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 5073 5257 1305
Size: 786 2982 184 2007
Fragments arranged by size:
2982 2007 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2233 10
Size: 54 1425 343 401 3736
Fragments arranged by size:
3736 1425 401 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2754 2838 3006 3174 3917 4276 4435
Size: 648 1588 84 168 168 743 359 159
Cuts at: 4435 518
Size: 2042
Fragments arranged by size:
2042 1588 743 648 359 168 168 159
84
MspA1I CmG'CkG
Cuts at: 1606 3375 4052 5200 5445 1606
Size: 1769 677 1148 245 2120
Fragments arranged by size:
2120 1769 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4468
Fragments arranged by size:
4468 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PfoI T'CCnGG_A
Cuts at: 2238 2238
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 3311 830
Size: 2481 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SacI G_AGCT'C
Cuts at: 2975 2975
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SexAI A'CCwGG_T
Cuts at: 2127 2127
Size: 5959
SfcI C'TryA_G
Cuts at: 826 1045 3307 4442 5123 5314 826
Size: 219 2262 1135 681 191 1471
Fragments arranged by size:
2262 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2638 4063 4964 5226 820
Size: 197 926 695 1425 901 262 1553
Fragments arranged by size:
1553 1425 926 901 695 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 3173 152
Size: 3021 2938
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 2876 1517
Size: 1359 4600
StyI C'CwwG_G
Cuts at: 513 1065 2004 3398 513
Size: 552 939 1394 3074
Fragments arranged by size:
3074 1394 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3986 4003 4156 4341 4786 325
Size: 1255 2406 17 153 185 445 1498
Fragments arranged by size:
2406 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3252 4205 5917
Size: 274 80 33 51 1443 1275 953 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
1712 1443 1275 953 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2456 2488 5919 5951 2456
Size: 32 3431 32 2464
Fragments arranged by size:
3431 2464 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI