Restriction map for
pFN21AE0934
(Circular) (Six-base) MAPSORT of: pFN21AE0934.seq Check: 1625 from: 1 to: 5953
pFN21AE0934 5953 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3088 3088
Size: 5953
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3763 278
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3296 1937
Size: 1359 4594
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 4080 4453 1563
Size: 2517 373 3063
Fragments arranged by size:
3063 2517 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2589 3189 4016 5400 5668 1632
Size: 957 600 827 1384 268 1917
Fragments arranged by size:
1917 1384 957 827 600 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5756
AflIII A'CryG_T
Cuts at: 1203 4852 5829 1203
Size: 3649 977 1327
Fragments arranged by size:
3649 1327 977
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 2611 4682 2611
Size: 2071 3882
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2169 1184
Size: 985 4968
AlwNI CAG_nnn'CTG
Cuts at: 3696 5268 3696
Size: 1572 4381
ApaLI G'TGCA_C
Cuts at: 4010 5166 4010
Size: 1156 4797
ApoI r'AATT_y
Cuts at: 1493 2385 2424 2819 2833 3221 3262 3488 3640
Size: 892 39 395 14 388 41 226 152
Cuts at: 3640 5749 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 892 395 388 226 152 41
39 14
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 3146 3278 1870
Size: 73 1203 132 4545
Fragments arranged by size:
4545 1203 132 73
BamHI G'GATC_C
Cuts at: 3283 3650 3283
Size: 367 5586
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3274 4729 618
Size: 325 217 627 1487 1455 1842
Fragments arranged by size:
1842 1487 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2930 3272 1391
Size: 1539 342 4072
Fragments arranged by size:
4072 1539 342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2205 2849 928
Size: 971 306 644 4032
Fragments arranged by size:
4032 971 644 306
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4144 4178 1903
Size: 34 2207 34 3678
Fragments arranged by size:
3678 2207 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5953
BfrBI ATG'CAT
Cuts at: 2671 2671
Size: 5953
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4564 136
Size: 107 122 71 631 605 2892 1525
Fragments arranged by size:
2892 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3037 5948 3037
Size: 2911 3042
BlpI GC'TnA_GC
Cuts at: 3370 3370
Size: 5953
Bme1580I G_kGCm'C
Cuts at: 1790 4014 5170 1790
Size: 2224 1156 2573
Fragments arranged by size:
2573 2224 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5953
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3684 4642 442
Size: 416 53 83 399 24 2267 958 1753
Fragments arranged by size:
2267 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3123 1056
Size: 2067 3886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4613 1239
Size: 3374 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2245 4078 4943 5241 2195
Size: 50 1833 865 298 2907
Fragments arranged by size:
2907 1833 865 298 50
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4616 882
Size: 617 3117 2219
Fragments arranged by size:
3117 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3744 493
Size: 711 2540 2702
Fragments arranged by size:
2702 2540 711
BsaBI GATnn'nnATC
Cuts at: 2670 5947 2670
Size: 3277 2676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3760 4142 4777 275
Size: 53 83 186 936 2227 382 635 1451
Fragments arranged by size:
2227 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4386 4784 5058 5205 5616 4386
Size: 398 274 147 411 4723
Fragments arranged by size:
4723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5728
Fragments arranged by size:
5728 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5953
BseYI C'CCAG_C
Cuts at: 2142 5156 5652 2142
Size: 3014 496 2443
Fragments arranged by size:
3014 2443 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5380
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3403 4164 5192 1540
Size: 275 186 1402 761 1028 2301
Fragments arranged by size:
2301 1402 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4014 4099 5170 4014
Size: 85 1071 4797
Fragments arranged by size:
4797 1071 85
BsiWI C'GTAC_G
Cuts at: 2989 2989
Size: 5953
BsmI GAATG_Cn'
Cuts at: 2410 3464 3557 2410
Size: 1054 93 4806
Fragments arranged by size:
4806 1054 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2930 3272 4014 4099 5170 1391
Size: 399 1140 342 742 85 1071 2174
Fragments arranged by size:
2174 1140 1071 742 399 342 85
BspEI T'CCGG_A
Cuts at: 5616 5616
Size: 5953
BspHI T'CATG_A
Cuts at: 3842 5572 3842
Size: 1730 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3088 3308 844
Size: 2244 220 3489
Fragments arranged by size:
3489 2244 220
BsrBI CCG'CTC
Cuts at: 1394 1823 3840 1394
Size: 429 2017 3507
Fragments arranged by size:
3507 2017 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4448 4622 65
Size: 1183 3200 174 1396
Fragments arranged by size:
3200 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 4597 4784 1700
Size: 110 207 2580 187 2869
Fragments arranged by size:
2869 2580 207 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 4013 5025 4013
Size: 1012 4941
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5953
BstBI TT'CG_AA
Cuts at: 4757 4757
Size: 5953
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5953
BstXI CCAn_nnnn'nTGG
Cuts at: 2036 2036
Size: 5953
BstYI r'GATC_y
Cuts at: 1972 2329 2776 3037 3283 3650 4035 4052 5504
Size: 357 447 261 246 367 385 17 1452
Cuts at: 5504 5590 5602 5948 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 447 385 367 357 346 261
246 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4462
Fragments arranged by size:
4462 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5214
Fragments arranged by size:
5214 683 56
BtsI GCAGTG_nn'
Cuts at: 3469 4263 4283 3469
Size: 794 20 5139
Fragments arranged by size:
5139 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5918
DraI TTT'AAA
Cuts at: 2274 2391 3257 2274
Size: 117 866 4970
Fragments arranged by size:
4970 866 117
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3400 4289 5918
Size: 54 1425 50 275 18 1570 889 1629
Cuts at: 5918 8
Size: 43
Fragments arranged by size:
1629 1570 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3883 1353
Size: 2530 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4540 4914 5060 387
Size: 1064 138 2951 374 146 1280
Fragments arranged by size:
2951 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2589 3189 4016 4613 5400 5668 1239
Size: 393 957 600 827 597 787 268 1524
Fragments arranged by size:
1524 957 827 787 600 597 393 268
EcoRI G'AATT_C
Cuts at: 3262 3640 3262
Size: 378 5575
FspI TGC'GCA
Cuts at: 4459 4459
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 5100 1678
Size: 3422 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2597 2629 2836 2868 2897
Size: 32 135 32 1025 32 207 32 29
Cuts at: 2897 2924 2929 2956 4594 4626 4668 4700 1373
Size: 27 5 27 1638 32 42 32 2626
Fragments arranged by size:
2626 1638 1025 207 135 42 32 32
32 32 32 32 29 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3297 3543 669
Size: 988 281 1359 246 3079
Fragments arranged by size:
3079 1359 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5953
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 3552 3552
Size: 5953
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2704 2738 5067 5251 1305
Size: 1399 34 2329 184 2007
Fragments arranged by size:
2329 2007 1399 184 34
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4131
Fragments arranged by size:
4131 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2904 3911 4270 4429 518
Size: 648 1738 1007 359 159 2042
Fragments arranged by size:
2042 1738 1007 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2509 3369 4046 5194 5439 1606
Size: 903 860 677 1148 245 2120
Fragments arranged by size:
2120 1148 903 860 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4462
Fragments arranged by size:
4462 939 552
NdeI CA'TA_TG
Cuts at: 387 2245 387
Size: 1858 4095
NheI G'CTAG_C
Cuts at: 1052 3119 1052
Size: 2067 3886
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NsiI A_TGCA'T
Cuts at: 2673 2673
Size: 5953
NspI r_CATG'y
Cuts at: 1132 1229 3311 4856 1132
Size: 97 2082 1545 2229
Fragments arranged by size:
2229 2082 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5953
PciI A'CATG_T
Cuts at: 4852 4852
Size: 5953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5953
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 5535 5567 4022
Size: 32 1481 32 4408
Fragments arranged by size:
4408 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 3523 3523
Size: 5953
PstI C_TGCA'G
Cuts at: 830 3305 830
Size: 2475 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
PvuII CAG'CTG
Cuts at: 2509 2509
Size: 5953
SalI G'TCGA_C
Cuts at: 1936 3295 1936
Size: 1359 4594
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 4201 5913 4201
Size: 1712 4241
SfcI C'TryA_G
Cuts at: 826 1045 2793 3301 4436 5117 5308 826
Size: 219 1748 508 1135 681 191 1471
Fragments arranged by size:
1748 1471 1135 681 508 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 2017 1700
Size: 317 5636
SmaI CCC'GGG
Cuts at: 1872 3280 1872
Size: 1408 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 2224 3146 4057 4958 5220
Size: 197 926 231 50 922 911 901 262
Cuts at: 5220 820
Size: 1553
Fragments arranged by size:
1553 926 922 911 901 262 231 197
50
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5953
StyI C'CwwG_G
Cuts at: 513 1065 2004 2643 3392 513
Size: 552 939 639 749 3074
Fragments arranged by size:
3074 939 749 639 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3980 3997 4150 4335 4780 325
Size: 1255 2400 17 153 185 445 1498
Fragments arranged by size:
2400 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3016 4199 5911
Size: 274 80 33 51 1443 1039 1183 1712
Cuts at: 5911 96
Size: 138
Fragments arranged by size:
1712 1443 1183 1039 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5913 5945 5913
Size: 32 5921
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5953
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XhoI C'TCGA_G
Cuts at: 1943 3146 1943
Size: 1203 4750
XmaI C'CCGG_G
Cuts at: 1870 3278 1870
Size: 1408 4545
XmnI GAAnn'nnTTC
Cuts at: 2202 2574 3222 4082 2202
Size: 372 648 860 4073
Fragments arranged by size:
4073 860 648 372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3761 276
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI