Restriction map for
pFN21AE0932
(Circular) (Six-base) MAPSORT of: pFN21AE0932.seq Check: 4509 from: 1 to: 5938
pFN21AE0932 5938 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3009 3281 1937
Size: 1072 272 4594
Fragments arranged by size:
4594 1072 272
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4001 5385 5653 1632
Size: 2369 1384 268 1917
Fragments arranged by size:
2369 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 4837 5814 1203
Size: 3634 977 1327
Fragments arranged by size:
3634 1327 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AlwNI CAG_nnn'CTG
Cuts at: 2570 3681 5253 2570
Size: 1111 1572 3255
Fragments arranged by size:
3255 1572 1111
ApaI G_GGCC'C
Cuts at: 2801 2801
Size: 5938
ApaLI G'TGCA_C
Cuts at: 2678 3995 5151 2678
Size: 1317 1156 3465
Fragments arranged by size:
3465 1317 1156
ApoI r'AATT_y
Cuts at: 1493 2618 2753 2825 3247 3473 3625 5734 1493
Size: 1125 135 72 422 226 152 2109 1697
Fragments arranged by size:
2109 1697 1125 422 226 152 135 72
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 2633 3263 1870
Size: 73 690 630 4545
Fragments arranged by size:
4545 690 630 73
BamHI G'GATC_C
Cuts at: 2459 3268 3635 2459
Size: 809 367 4762
Fragments arranged by size:
4762 809 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2416 3259 4714 618
Size: 325 217 627 629 843 1455 1842
Fragments arranged by size:
1842 1455 843 629 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2801 3257 1391
Size: 678 732 456 4072
Fragments arranged by size:
4072 732 678 456
BbeI G_GCGC'C
Cuts at: 2420 2420
Size: 5938
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4967
BbvCI CC'TCA_GC
Cuts at: 2338 2338
Size: 5938
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3136 3830 5040 3136
Size: 694 1210 4034
Fragments arranged by size:
4034 1210 694
BclI T'GATC_A
Cuts at: 1548 3045 1548
Size: 1497 4441
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4549 136
Size: 107 122 71 631 605 2877 1525
Fragments arranged by size:
2877 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5933 5933
Size: 5938
BlpI GC'TnA_GC
Cuts at: 3355 3355
Size: 5938
Bme1580I G_kGCm'C
Cuts at: 1790 2682 2801 3999 5155 1790
Size: 892 119 1198 1156 2573
Fragments arranged by size:
2573 1198 1156 892 119
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5938
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3669 4627 442
Size: 416 53 83 399 24 2252 958 1753
Fragments arranged by size:
2252 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2083 4598 1239
Size: 844 2515 2579
Fragments arranged by size:
2579 2515 844
Bpu10I CC'TnA_GC
Cuts at: 2338 2338
Size: 5938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4063 4928 5226 4063
Size: 865 298 4775
Fragments arranged by size:
4775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2640 4601 882
Size: 617 1141 1961 2219
Fragments arranged by size:
2219 1961 1141 617
BsaAI yAC'GTr
Cuts at: 493 1204 3729 493
Size: 711 2525 2702
Fragments arranged by size:
2702 2525 711
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2417 3745 4127 4762
Size: 53 83 186 936 884 1328 382 635
Cuts at: 4762 275
Size: 1451
Fragments arranged by size:
1451 1328 936 884 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4371 4769 5043 5190 5601 4371
Size: 398 274 147 411 4708
Fragments arranged by size:
4708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5713
Fragments arranged by size:
5713 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2137 1186
Size: 951 4987
BseYI C'CCAG_C
Cuts at: 2377 2554 2864 5141 5637 2377
Size: 177 310 2277 496 2678
Fragments arranged by size:
2678 2277 496 310 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2507 1163
Size: 573 771 4594
Fragments arranged by size:
4594 771 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3388 4149 5177 1540
Size: 275 186 1387 761 1028 2301
Fragments arranged by size:
2301 1387 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2682 3208 3999 4084 5155 2682
Size: 526 791 85 1071 3465
Fragments arranged by size:
3465 1071 791 526 85
BsmI GAATG_Cn'
Cuts at: 3449 3542 3449
Size: 93 5845
BsmBI CGTCTCn'nnnn_
Cuts at: 2356 2966 2356
Size: 610 5328
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2682 2801 3208 3257 3999 4084
Size: 399 279 613 119 407 49 742 85
Cuts at: 4084 5155 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 613 407 399 279 119
85 49
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 3827 5557 3827
Size: 1730 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3293 844
Size: 2449 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3024 3825 1394
Size: 429 1201 801 3507
Fragments arranged by size:
3507 1201 801 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2714 4433 4607 65
Size: 1183 1466 1719 174 1396
Fragments arranged by size:
1719 1466 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4582 4769 1700
Size: 110 2772 187 2869
Fragments arranged by size:
2869 2772 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 2265 3998 5010 2265
Size: 1733 1012 3193
Fragments arranged by size:
3193 1733 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 4742 4742
Size: 5938
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstYI r'GATC_y
Cuts at: 1972 2459 3268 3635 4020 4037 5489 5575 5587
Size: 487 809 367 385 17 1452 86 12
Cuts at: 5587 5933 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 809 487 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3010 3010
Size: 5938
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4447
Fragments arranged by size:
4447 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5199
Fragments arranged by size:
5199 683 56
BtsI GCAGTG_nn'
Cuts at: 2358 3454 4248 4268 2358
Size: 1096 794 20 4028
Fragments arranged by size:
4028 1096 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 2841 3242 2841
Size: 401 5537
DraIII CAC_nnn'GTG
Cuts at: 2117 2117
Size: 5938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 2355 2429
Size: 54 1425 50 275 18 498 27 74
Cuts at: 2429 2998 3385 4274 5903 8
Size: 569 387 889 1629 43
Fragments arranged by size:
1629 1425 889 569 498 387 275 74
54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2678 3868 1353
Size: 1325 1190 3423
Fragments arranged by size:
3423 1325 1190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4525 4899 5045 387
Size: 1064 138 2936 374 146 1280
Fragments arranged by size:
2936 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2083 4001 4598 5385 5653 1239
Size: 393 451 1918 597 787 268 1524
Fragments arranged by size:
1918 1524 787 597 451 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2095 2311 2797 2798 2095
Size: 216 486 1 5235
Fragments arranged by size:
5235 486 216 1
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 2420 5085 1678
Size: 742 2665 2531
Fragments arranged by size:
2665 2531 742
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2531 2563 2639 2671 4579
Size: 32 135 32 959 32 76 32 1908
Cuts at: 4579 4611 4653 4685 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1908 959 135 76 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3282 3528 669
Size: 988 281 1344 246 3079
Fragments arranged by size:
3079 1344 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2083 2603 748
Size: 1335 520 4083
Fragments arranged by size:
4083 1335 520
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KasI G'GCGC_C
Cuts at: 2416 2416
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 2037 3537 2037
Size: 1500 4438
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5052 5236 1305
Size: 3747 184 2007
Fragments arranged by size:
3747 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4116
Fragments arranged by size:
4116 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3896 4255 4414 518
Size: 648 2730 359 159 2042
Fragments arranged by size:
2730 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2513 3354 4031 5179 5424 1606
Size: 907 841 677 1148 245 2120
Fragments arranged by size:
2120 1148 907 841 677 245
NarI GG'CG_CC
Cuts at: 2417 2417
Size: 5938
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4447
Fragments arranged by size:
4447 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 2693 3296 4841 1132
Size: 97 1464 603 1545 2229
Fragments arranged by size:
2229 1545 1464 603 97
PasI CC'CwG_GG
Cuts at: 1348 2411 1348
Size: 1063 4875
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2117 1108
Size: 1009 4929
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2736 1613
Size: 1123 4815
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PspOMI G'GGCC_C
Cuts at: 2797 2797
Size: 5938
PstI C_TGCA'G
Cuts at: 830 2029 2319 3290 830
Size: 1199 290 971 3478
Fragments arranged by size:
3478 1199 971 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SfcI C'TryA_G
Cuts at: 826 1045 2025 2315 3286 4421 5102 5293 826
Size: 219 980 290 971 1135 681 191 1471
Fragments arranged by size:
1471 1135 980 971 681 290 219 191
SfoI GGC'GCC
Cuts at: 2418 2418
Size: 5938
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2633 4042 4943 5205 820
Size: 197 926 690 1409 901 262 1553
Fragments arranged by size:
1553 1409 926 901 690 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 2693 3296 1132
Size: 1561 603 3774
Fragments arranged by size:
3774 1561 603
SspI AAT'ATT
Cuts at: 5 52 2851 3862 5
Size: 47 2799 1011 2081
Fragments arranged by size:
2799 2081 1011 47
StuI AGG'CCT
Cuts at: 1517 2495 1517
Size: 978 4960
StyI C'CwwG_G
Cuts at: 513 1065 2004 3377 513
Size: 552 939 1373 3074
Fragments arranged by size:
3074 1373 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3965 3982 4135 4320 4765 325
Size: 1255 2385 17 153 185 445 1498
Fragments arranged by size:
2385 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4184 5896 96
Size: 274 80 33 51 1443 2207 1712 138
Fragments arranged by size:
2207 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2251 2283 5898 5930 2251
Size: 32 3615 32 2259
Fragments arranged by size:
3615 2259 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5938
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2608 2608
Size: 5938
XhoI C'TCGA_G
Cuts at: 1943 2633 1943
Size: 690 5248
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 4067 4067
Size: 5938
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI