Restriction map for
pFN21AE0931
(Circular) (Six-base) MAPSORT of: pFN21AE0931.seq Check: 2740 from: 1 to: 5875
pFN21AE0931 5875 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3218 1937
Size: 1281 4594
Acc65I G'GTAC_C
Cuts at: 3196 3196
Size: 5875
AclI AA'CG_TT
Cuts at: 1563 2396 2799 4002 4375 1563
Size: 833 403 1203 373 3063
Fragments arranged by size:
3063 1203 833 403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2146 2198 2481 2705 3048 3938 5322 5590
Size: 514 52 283 224 343 890 1384 268
Cuts at: 5590 1632
Size: 1917
Fragments arranged by size:
1917 1384 890 514 343 283 268 224
52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5875
AlwNI CAG_nnn'CTG
Cuts at: 2147 2194 2814 2827 3618 5190 2147
Size: 47 620 13 791 1572 2832
Fragments arranged by size:
2832 1572 791 620 47 13
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 3184 3410 3562 5671 1493
Size: 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 2130 3200 1870
Size: 73 187 1070 4545
Fragments arranged by size:
4545 1070 187 73
BamHI G'GATC_C
Cuts at: 2321 2452 2566 2733 3205 3572 2321
Size: 131 114 167 472 367 4624
Fragments arranged by size:
4624 472 367 167 131 114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3196 4651 618
Size: 325 217 627 1409 1455 1842
Fragments arranged by size:
1842 1455 1409 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2721 3194 1391
Size: 1330 473 4072
Fragments arranged by size:
4072 1330 473
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4904
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2627 2661 4066 4100 1903
Size: 34 690 34 1405 34 3678
Fragments arranged by size:
3678 1405 690 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4486 136
Size: 107 122 71 631 605 2814 1525
Fragments arranged by size:
2814 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5870 5870
Size: 5875
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 3936 5092 1790
Size: 2146 1156 2573
Fragments arranged by size:
2573 2146 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3606 4564 442
Size: 416 53 83 399 24 2189 958 1753
Fragments arranged by size:
2189 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2262 4535 1239
Size: 1023 2273 2579
Fragments arranged by size:
2579 2273 1023
Bpu10I CC'TnA_GC
Cuts at: 2608 2608
Size: 5875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2366 2713 3123 4000 4865 5163 2366
Size: 347 410 877 865 298 3078
Fragments arranged by size:
3078 877 865 410 347 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4538 882
Size: 617 3039 2219
Fragments arranged by size:
3039 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3666 493
Size: 711 2462 2702
Fragments arranged by size:
2702 2462 711
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3039 3069 1178
Size: 30 165 30 1636 30 3984
Fragments arranged by size:
3984 1636 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2067 2079 3131 1186
Size: 881 12 1052 3930
Fragments arranged by size:
3930 1052 881 12
BseYI C'CCAG_C
Cuts at: 5078 5574 5078
Size: 496 5379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5302
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3325 4086 5114 1540
Size: 275 186 1324 761 1028 2301
Fragments arranged by size:
2301 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2449 3936 4021 5092 2449
Size: 1487 85 1071 3232
Fragments arranged by size:
3232 1487 1071 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2449 2721 3194 3936 4021 5092 1391
Size: 399 659 272 473 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 659 473 399 272 85
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 2907 3764 5494 2907
Size: 857 1730 3288
Fragments arranged by size:
3288 1730 857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3230 844
Size: 2386 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3762 1394
Size: 429 1939 3507
Fragments arranged by size:
3507 1939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4370 4544 65
Size: 1183 3122 174 1396
Fragments arranged by size:
3122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4519 4706 1700
Size: 110 2709 187 2869
Fragments arranged by size:
2869 2709 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 3935 4947 3935
Size: 1012 4863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5875
BstBI TT'CG_AA
Cuts at: 2557 4679 2557
Size: 2122 3753
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5875
BstYI r'GATC_y
Cuts at: 1972 2321 2452 2566 2733 2988 3205 3572 3957
Size: 349 131 114 167 255 217 367 385
Cuts at: 3957 3974 5426 5512 5524 5870 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 349 346 255 217
167 131 114 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2023 513
Size: 552 958 4365
Fragments arranged by size:
4365 958 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 3391 4185 4205 3391
Size: 794 20 5061
Fragments arranged by size:
5061 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DraIII CAC_nnn'GTG
Cuts at: 2359 2975 2359
Size: 616 5259
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2995 3322 4211
Size: 54 1425 50 275 18 1165 327 889
Cuts at: 4211 5840 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1165 889 327 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3322 1537
Size: 275 1510 4090
Fragments arranged by size:
4090 1510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2174 3108 3805 1353
Size: 821 934 697 3423
Fragments arranged by size:
3423 934 821 697
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4462 4836 4982 387
Size: 1064 138 2873 374 146 1280
Fragments arranged by size:
2873 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2198 2262 2481 2705 3048 3938
Size: 393 514 52 64 219 224 343 890
Cuts at: 3938 4535 5322 5590 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 890 787 597 514 393 343 268
224 219 64 52
EcoNI CCTnn'n_nnAGG
Cuts at: 2996 2996
Size: 5875
EcoO109I rG'GnC_Cy
Cuts at: 2076 2191 2076
Size: 115 5760
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
EcoRV GAT'ATC
Cuts at: 2587 2587
Size: 5875
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 2041 2815 5022 1678
Size: 363 774 2207 2531
Fragments arranged by size:
2531 2207 774 363
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2248 2260 2280 2292 4516
Size: 32 135 32 676 12 20 12 2224
Cuts at: 4516 4548 4590 4622 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2224 676 135 42 32 32 32
32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 3200 3200
Size: 5875
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 2644 4989 5173 1305
Size: 1074 265 2345 184 2007
Fragments arranged by size:
2345 2007 1074 265 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2997 10
Size: 54 1425 343 1165 2888
Fragments arranged by size:
2888 1425 1165 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2906 3833 4192 4351 518
Size: 648 1740 927 359 159 2042
Fragments arranged by size:
2042 1740 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2631 3291 3968 5116 5361 1606
Size: 1025 660 677 1148 245 2120
Fragments arranged by size:
2120 1148 1025 677 660 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5323
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 2627 3233 4778 1132
Size: 97 1398 606 1545 2229
Fragments arranged by size:
2229 1545 1398 606 97
PasI CC'CwG_GG
Cuts at: 1348 2416 1348
Size: 1068 4807
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5875
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2191
Size: 5875
PshAI GACnn'nnGTC
Cuts at: 1613 2954 1613
Size: 1341 4534
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PstI C_TGCA'G
Cuts at: 830 2836 3227 830
Size: 2006 391 3478
Fragments arranged by size:
3478 2006 391
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SexAI A'CCwGG_T
Cuts at: 2367 2367
Size: 5875
SfcI C'TryA_G
Cuts at: 826 1045 2346 2517 2832 3223 4358 5039 5230
Size: 219 1301 171 315 391 1135 681 191
Cuts at: 5230 826
Size: 1471
Fragments arranged by size:
1471 1301 1135 681 391 315 219 191
171
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 2132 3202 1872
Size: 260 1070 4545
Fragments arranged by size:
4545 1070 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2381 2692 3138 3979 4880 5142
Size: 197 926 438 311 446 841 901 262
Cuts at: 5142 820
Size: 1553
Fragments arranged by size:
1553 926 901 841 446 438 311 262
197
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 2171 1517
Size: 654 5221
StyI C'CwwG_G
Cuts at: 513 1065 2992 3314 513
Size: 552 1927 322 3074
Fragments arranged by size:
3074 1927 552 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2563 3902 3919 4072 4257 4702 325
Size: 1255 983 1339 17 153 185 445 1498
Fragments arranged by size:
1498 1339 1255 983 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2896 4121 5833
Size: 274 80 33 51 1443 919 1225 1712
Cuts at: 5833 96
Size: 138
Fragments arranged by size:
1712 1443 1225 919 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2343 2375 2718 2750 5835 5867 2343
Size: 32 343 32 3085 32 2351
Fragments arranged by size:
3085 2351 343 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2930 1316
Size: 1614 4261
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2785 2785
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 2130 3200 1870
Size: 260 1070 4545
Fragments arranged by size:
4545 1070 260
XmnI GAAnn'nnTTC
Cuts at: 2307 4004 2307
Size: 1697 4178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI