Restriction map for
pFN21AE0929
(Circular) (Six-base) MAPSORT of: pFN21AE0929.seq Check: 4144 from: 1 to: 5860
pFN21AE0929 5860 bp
With 182 enzymes: SgfI *
March 28, 2012 13:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2867 2871 3923 5307 5575 1632
Size: 1235 4 1052 1384 268 1917
Fragments arranged by size:
1917 1384 1235 1052 268 4
AfeI AGC'GCT
Cuts at: 2630 2630
Size: 5860
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 2185 4759 5736 1203
Size: 982 2574 977 1327
Fragments arranged by size:
2574 1327 982 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 2684 3128 1166
Size: 1518 444 3898
Fragments arranged by size:
3898 1518 444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2636 2668 2636
Size: 32 5828
AlwNI CAG_nnn'CTG
Cuts at: 2838 2932 2992 3603 5175 2838
Size: 94 60 611 1572 3523
Fragments arranged by size:
3523 1572 611 94 60
ApaI G_GGCC'C
Cuts at: 2143 2174 2143
Size: 31 5829
ApaLI G'TGCA_C
Cuts at: 2447 3917 5073 2447
Size: 1470 1156 3234
Fragments arranged by size:
3234 1470 1156
ApoI r'AATT_y
Cuts at: 1493 2234 3169 3395 3547 5656 1493
Size: 741 935 226 152 2109 1697
Fragments arranged by size:
2109 1697 935 741 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 3007 3185 1870
Size: 73 1064 178 4545
Fragments arranged by size:
4545 1064 178 73
AvrII C'CTAG_G
Cuts at: 3016 3016
Size: 5860
BamHI G'GATC_C
Cuts at: 3021 3190 3557 3021
Size: 169 367 5324
Fragments arranged by size:
5324 367 169
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2477 2852 3181 4636 618
Size: 325 217 627 690 375 329 1455 1842
Fragments arranged by size:
1842 1455 690 627 375 329 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2143 2160 2174 3007 3114 3179 1391
Size: 700 52 17 14 833 107 65 4072
Fragments arranged by size:
4072 833 700 107 65 52 17 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2593 928
Size: 971 694 4195
Fragments arranged by size:
4195 971 694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4051 4085 1903
Size: 34 2114 34 3678
Fragments arranged by size:
3678 2114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2164 4471 136
Size: 107 122 71 631 605 492 2307 1525
Fragments arranged by size:
2307 1525 631 605 492 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2174 2252 2451 2482 3921 5077 1790
Size: 353 31 78 199 31 1439 1156 2573
Fragments arranged by size:
2573 1439 1156 353 199 78 31 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2145 2172 2476
Size: 416 53 83 399 24 728 27 304
Cuts at: 2476 2668 3591 4549 442
Size: 192 923 958 1753
Fragments arranged by size:
1753 958 923 728 416 399 304 192
83 53 27 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 3021 4520 1239
Size: 992 790 1499 2579
Fragments arranged by size:
2579 1499 992 790
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 3985 4850 5148 2501
Size: 1484 865 298 3213
Fragments arranged by size:
3213 1484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2045 2663 4523 882
Size: 617 546 618 1860 2219
Fragments arranged by size:
2219 1860 618 617 546
BsaAI yAC'GTr
Cuts at: 493 1204 2186 2596 2982 3651 493
Size: 711 982 410 386 669 2702
Fragments arranged by size:
2702 982 711 669 410 386
BsaBI GATnn'nnATC
Cuts at: 3075 5854 3075
Size: 2779 3081
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3667 4049 4684 275
Size: 53 83 186 936 2134 382 635 1451
Fragments arranged by size:
2134 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4293 4691 4965 5112 5523 4293
Size: 398 274 147 411 4630
Fragments arranged by size:
4630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2756 2786 1178
Size: 30 165 30 1353 30 4252
Fragments arranged by size:
4252 1353 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5860
BseYI C'CCAG_C
Cuts at: 2082 2095 2245 2428 2715 2809 3097 3101 3106
Size: 13 150 183 287 94 288 4 5
Cuts at: 3106 5063 5559 2082
Size: 1957 496 2383
Fragments arranged by size:
2383 1957 496 288 287 183 150 94
13 5 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5287
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2451 3921 4006 5077 2451
Size: 1470 85 1071 3234
Fragments arranged by size:
3234 1470 1071 85
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2143 2160 2174 2252 2451 2482
Size: 399 301 52 17 14 78 199 31
Cuts at: 2482 3007 3114 3179 3921 4006 5077 1391
Size: 525 107 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 525 399 301 199 107
85 78 65 52 31 17 14
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2541 3215 844
Size: 1697 674 3489
Fragments arranged by size:
3489 1697 674
BsrBI CCG'CTC
Cuts at: 1394 1823 3747 1394
Size: 429 1924 3507
Fragments arranged by size:
3507 1924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 4529 65
Size: 1183 3107 174 1396
Fragments arranged by size:
3107 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4504 4691 1700
Size: 110 2694 187 2869
Fragments arranged by size:
2869 2694 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 2100 1184
Size: 916 4944
BstYI r'GATC_y
Cuts at: 1972 2307 3021 3190 3557 3942 3959 5411 5497
Size: 335 714 169 367 385 17 1452 86
Cuts at: 5497 5509 5855 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 714 385 367 346 335 169
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2943 2943
Size: 5860
BtgI C'CryG_G
Cuts at: 513 1065 2004 2789 513
Size: 552 939 785 3584
Fragments arranged by size:
3584 939 785 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 2691 3376 4170 4190 2691
Size: 685 794 20 4361
Fragments arranged by size:
4361 794 685 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 2328 2440
Size: 54 1425 50 275 18 321 177 112
Cuts at: 2440 2548 2961 3145 3307 4196 5825 8
Size: 108 413 184 162 889 1629 43
Fragments arranged by size:
1629 1425 889 413 321 275 184 177
162 112 108 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3790 1353
Size: 2437 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2867 2871 3021 3923 4520 5307
Size: 393 599 636 4 150 902 597 787
Cuts at: 5307 5575 1239
Size: 268 1524
Fragments arranged by size:
1524 902 787 636 599 597 393 268
150 4
EcoO109I rG'GnC_Cy
Cuts at: 2117 2139 2170 2171 2947 3030 3140 2117
Size: 22 31 1 776 83 110 4837
Fragments arranged by size:
4837 776 110 83 31 22 1
EcoRI G'AATT_C
Cuts at: 2234 3169 3547 2234
Size: 935 378 4547
Fragments arranged by size:
4547 935 378
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2632 5007 1678
Size: 954 2375 2531
Fragments arranged by size:
2531 2375 954
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2206 2238 2680 2712 2756
Size: 32 135 32 634 32 442 32 44
Cuts at: 2756 2788 4501 4533 4575 4607 1373
Size: 32 1713 32 42 32 2626
Fragments arranged by size:
2626 1713 634 442 135 44 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2883 748
Size: 2135 3725
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2550 3147 10
Size: 54 1425 343 718 597 2723
Fragments arranged by size:
2723 1425 718 597 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2684 3128 3818 4177 4336 518
Size: 648 1089 429 444 690 359 159 2042
Fragments arranged by size:
2042 1089 690 648 444 429 359 159
MspA1I CmG'CkG
Cuts at: 1606 2209 2245 2719 2929 3101 3276 3953 5101
Size: 603 36 474 210 172 175 677 1148
Cuts at: 5101 5346 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 603 474 245 210 175
172 36
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4369
Fragments arranged by size:
4369 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 3218 4763 1132
Size: 97 1989 1545 2229
Fragments arranged by size:
2229 1989 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2149 1108
Size: 1041 4819
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PmlI CAC'GTG
Cuts at: 2186 2596 2186
Size: 410 5450
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2187 2219 2660 2692 3929 3961 5442 5474 2187
Size: 32 441 32 1237 32 1481 32 2573
Fragments arranged by size:
2573 1481 1237 441 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2947 3030 2947
Size: 83 5777
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PspOMI G'GGCC_C
Cuts at: 2139 2170 2139
Size: 31 5829
PstI C_TGCA'G
Cuts at: 830 3212 830
Size: 2382 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2209 2245 2719 2929 3101 2209
Size: 36 474 210 172 4968
Fragments arranged by size:
4968 474 210 172 36
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2968 3208 4343 5024 5215 826
Size: 219 1923 240 1135 681 191 1471
Fragments arranged by size:
1923 1471 1135 681 240 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2516 3964 4865 5127 820
Size: 197 926 573 1448 901 262 1553
Fragments arranged by size:
1553 1448 926 901 573 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 2974 3087 1517
Size: 1457 113 4290
Fragments arranged by size:
4290 1457 113
StyI C'CwwG_G
Cuts at: 513 1065 2004 2268 2472 3016 3026 3299 513
Size: 552 939 264 204 544 10 273 3074
Fragments arranged by size:
3074 939 552 544 273 264 204 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2729 3887 3904 4057 4242 4687 325
Size: 1255 1149 1158 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1158 1149 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2994 4106 5818
Size: 274 80 33 51 1443 1017 1112 1712
Cuts at: 5818 96
Size: 138
Fragments arranged by size:
1712 1443 1112 1017 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2931 2963 5820 5852 2931
Size: 32 2857 32 2939
Fragments arranged by size:
2939 2857 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2533 2533
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI