Restriction map for
pFN21AE0921
(Circular) (Six-base) MAPSORT of: pFN21AE0921.seq Check: 3758 from: 1 to: 5551
pFN21AE0921 5551 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3361 278
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2894 1937
Size: 957 4594
Acc65I G'GTAC_C
Cuts at: 2039 2872 2039
Size: 833 4718
AclI AA'CG_TT
Cuts at: 1563 3678 4051 1563
Size: 2115 373 3063
Fragments arranged by size:
3063 2115 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3614 4998 5266 1632
Size: 1982 1384 268 1917
Fragments arranged by size:
1982 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5354
AflIII A'CryG_T
Cuts at: 1203 2213 4450 5427 1203
Size: 1010 2237 977 1327
Fragments arranged by size:
2237 1327 1010 977
AgeI A'CCGG_T
Cuts at: 2036 4382 2036
Size: 2346 3205
AhdI GACnn_n'nnGTC
Cuts at: 4280 4280
Size: 5551
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5551
AlwNI CAG_nnn'CTG
Cuts at: 3294 4866 3294
Size: 1572 3979
ApaI G_GGCC'C
Cuts at: 2237 2237
Size: 5551
ApaLI G'TGCA_C
Cuts at: 3608 4764 3608
Size: 1156 4395
ApoI r'AATT_y
Cuts at: 1493 2662 2860 3086 3238 5347 1493
Size: 1169 198 226 152 2109 1697
Fragments arranged by size:
2109 1697 1169 226 198 152
AseI AT'TA_AT
Cuts at: 160 1032 2514 4105 160
Size: 872 1482 1591 1606
Fragments arranged by size:
1606 1591 1482 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
AvaI C'yCGr_G
Cuts at: 1870 1943 2876 1870
Size: 73 933 4545
Fragments arranged by size:
4545 933 73
BamHI G'GATC_C
Cuts at: 2243 2881 3248 2243
Size: 638 367 4546
Fragments arranged by size:
4546 638 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2068 2872 4327 618
Size: 325 217 627 252 29 804 1455 1842
Fragments arranged by size:
1842 1455 804 627 325 252 217 29
BanII G_rGCy'C
Cuts at: 1391 2237 2364 2870 1391
Size: 846 127 506 4072
Fragments arranged by size:
4072 846 506 127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 928
Size: 971 402 4178
Fragments arranged by size:
4178 971 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3742 3776 1903
Size: 34 1805 34 3678
Fragments arranged by size:
3678 1805 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3443 4653 3443
Size: 1210 4341
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5551
BfrBI ATG'CAT
Cuts at: 2452 2452
Size: 5551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4162 136
Size: 107 122 71 631 605 2490 1525
Fragments arranged by size:
2490 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5546 5546
Size: 5551
BlpI GC'TnA_GC
Cuts at: 2968 2968
Size: 5551
Bme1580I G_kGCm'C
Cuts at: 1790 2237 2396 3612 4768 1790
Size: 447 159 1216 1156 2573
Fragments arranged by size:
2573 1216 1156 447 159
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5551
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2545 3282 4240
Size: 416 53 83 399 24 1128 737 958
Cuts at: 4240 442
Size: 1753
Fragments arranged by size:
1753 1128 958 737 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4211 1239
Size: 2972 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2699 3676 4541 4839 2699
Size: 977 865 298 3411
Fragments arranged by size:
3411 977 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4214 882
Size: 617 2715 2219
Fragments arranged by size:
2715 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3342 493
Size: 711 2138 2702
Fragments arranged by size:
2702 2138 711
BsaBI GATnn'nnATC
Cuts at: 5545 5545
Size: 5551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3358 3740 4375 275
Size: 53 83 186 936 1825 382 635 1451
Fragments arranged by size:
1825 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2036 3984 4382 4656 4803 5214 2036
Size: 1948 398 274 147 411 2373
Fragments arranged by size:
2373 1948 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5326
Fragments arranged by size:
5326 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2712 1186
Size: 1526 4025
BseYI C'CCAG_C
Cuts at: 4754 5250 4754
Size: 496 5055
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4978
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3001 3762 4790 1540
Size: 275 186 1000 761 1028 2301
Fragments arranged by size:
2301 1028 1000 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 3612 3697 4768 2364
Size: 1248 85 1071 3147
Fragments arranged by size:
3147 1248 1071 85
BsmI GAATG_Cn'
Cuts at: 3062 3155 3062
Size: 93 5458
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2364 2396 2870 3612 3697 4768
Size: 399 447 127 32 474 742 85 1071
Cuts at: 4768 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 474 447 399 127 85
32
BspEI T'CCGG_A
Cuts at: 5214 5214
Size: 5551
BspHI T'CATG_A
Cuts at: 2734 3440 5170 2734
Size: 706 1730 3115
Fragments arranged by size:
3115 1730 706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2906 844
Size: 2062 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3438 1394
Size: 429 1615 3507
Fragments arranged by size:
3507 1615 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2790 4046 4220 65
Size: 1183 1542 1256 174 1396
Fragments arranged by size:
1542 1396 1256 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 4195 4382 1700
Size: 110 226 2159 187 2869
Fragments arranged by size:
2869 2159 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5551
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5551
BssSI C'ACGA_G
Cuts at: 3611 4623 3611
Size: 1012 4539
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5551
BstBI TT'CG_AA
Cuts at: 4355 4355
Size: 5551
BstEII G'GTnAC_C
Cuts at: 1184 2606 1184
Size: 1422 4129
BstYI r'GATC_y
Cuts at: 1972 2243 2881 3248 3633 3650 5102 5188 5200
Size: 271 638 367 385 17 1452 86 12
Cuts at: 5200 5546 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 638 385 367 346 271 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4999
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4812
Fragments arranged by size:
4812 683 56
BtsI GCAGTG_nn'
Cuts at: 3067 3861 3881 3067
Size: 794 20 4737
Fragments arranged by size:
4737 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5516
DraI TTT'AAA
Cuts at: 2855 2855
Size: 5551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2774 4558 809
Size: 507 1458 1784 1802
Fragments arranged by size:
1802 1784 1458 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2176 2998 3887
Size: 54 1425 50 275 18 346 822 889
Cuts at: 3887 5516 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 822 346 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2998 1537
Size: 275 1186 4090
Fragments arranged by size:
4090 1186 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 3481 1353
Size: 999 1129 3423
Fragments arranged by size:
3423 1129 999
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4138 4512 4658 387
Size: 1064 138 2549 374 146 1280
Fragments arranged by size:
2549 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2362 2362
Size: 5551
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3614 4211 4998 5266 1239
Size: 393 1982 597 787 268 1524
Fragments arranged by size:
1982 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2233 2234 2233
Size: 1 5550
EcoRI G'AATT_C
Cuts at: 2860 3238 2860
Size: 378 5173
FspI TGC'GCA
Cuts at: 4057 4057
Size: 5551
HaeII r_GCGC'y
Cuts at: 1678 4698 1678
Size: 3020 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2196 4192 4224 4266
Size: 32 135 32 592 32 1996 32 42
Cuts at: 4266 4298 1373
Size: 32 2626
Fragments arranged by size:
2626 1996 592 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2533 2895 3141 669
Size: 988 281 595 362 246 3079
Fragments arranged by size:
3079 988 595 362 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5551
HpaI GTT'AAC
Cuts at: 3141 3141
Size: 5551
KpnI G_GTAC'C
Cuts at: 2043 2876 2043
Size: 833 4718
MfeI C'AATT_G
Cuts at: 3150 3150
Size: 5551
MluI A'CGCG_T
Cuts at: 5427 5427
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 2246 4665 4849 1305
Size: 816 125 2419 184 2007
Fragments arranged by size:
2419 2007 816 184 125
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2178 10
Size: 54 1425 343 346 3383
Fragments arranged by size:
3383 1425 346 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3509 3868 4027 518
Size: 648 2343 359 159 2042
Fragments arranged by size:
2343 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2635 2967 3644 4792 5037 1606
Size: 1029 332 677 1148 245 2120
Fragments arranged by size:
2120 1148 1029 677 332 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4999
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5551
NotI GC'GGCC_GC
Cuts at: 2998 2998
Size: 5551
NsiI A_TGCA'T
Cuts at: 2454 2454
Size: 5551
NspI r_CATG'y
Cuts at: 1132 1229 2217 2909 4454 1132
Size: 97 988 692 1545 2229
Fragments arranged by size:
2229 1545 988 692 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5551
PciI A'CATG_T
Cuts at: 2213 4450 2213
Size: 2237 3314
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5551
PmeI GTTT'AAAC
Cuts at: 2855 2855
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 5133 5165 3620
Size: 32 1481 32 4006
Fragments arranged by size:
4006 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5551
PsiI TTA'TAA
Cuts at: 3121 3121
Size: 5551
PspOMI G'GGCC_C
Cuts at: 2233 2233
Size: 5551
PstI C_TGCA'G
Cuts at: 830 2903 830
Size: 2073 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5551
PvuII CAG'CTG
Cuts at: 2635 2635
Size: 5551
SacI G_AGCT'C
Cuts at: 2364 2364
Size: 5551
SalI G'TCGA_C
Cuts at: 1936 2893 1936
Size: 957 4594
SapI GCTCTTCn'nnn_
Cuts at: 2352 2352
Size: 5551
SbfI CC_TGCA'GG
Cuts at: 2903 2903
Size: 5551
ScaI AGT'ACT
Cuts at: 2286 3799 5511 2286
Size: 1513 1712 2326
Fragments arranged by size:
2326 1712 1513
SfcI C'TryA_G
Cuts at: 826 1045 2022 2477 2505 2899 4034 4715 4906
Size: 219 977 455 28 394 1135 681 191
Cuts at: 4906 826
Size: 1471
Fragments arranged by size:
1471 1135 977 681 455 394 219 191
28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5551
SmaI CCC'GGG
Cuts at: 1872 2878 1872
Size: 1006 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2678 3655 4556 4818 820
Size: 197 926 735 977 901 262 1553
Fragments arranged by size:
1553 977 926 901 735 262 197
SnaBI TAC'GTA
Cuts at: 493 3342 493
Size: 2849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5551
SphI G_CATG'C
Cuts at: 1132 2909 1132
Size: 1777 3774
SspI AAT'ATT
Cuts at: 5 52 3475 5
Size: 47 3423 2081
Fragments arranged by size:
3423 2081 47
StuI AGG'CCT
Cuts at: 1517 2377 1517
Size: 860 4691
StyI C'CwwG_G
Cuts at: 513 1065 2990 513
Size: 552 1925 3074
Fragments arranged by size:
3074 1925 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2527 3578 3595 3748 3933 4378 325
Size: 1255 947 1051 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1051 947 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2284 3797 5509
Size: 274 80 33 51 1443 307 1513 1712
Cuts at: 5509 96
Size: 138
Fragments arranged by size:
1712 1513 1443 307 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2203 2235 5511 5543 2203
Size: 32 3276 32 2211
Fragments arranged by size:
3276 2211 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5551
XbaI T'CTAG_A
Cuts at: 2887 2887
Size: 5551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2104 2254 2104
Size: 150 5401
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5551
XmaI C'CCGG_G
Cuts at: 1870 2876 1870
Size: 1006 4545
XmnI GAAnn'nnTTC
Cuts at: 3680 3680
Size: 5551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3359 276
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI