Restriction map for
pFN21AE0920
(Circular) (Six-base) MAPSORT of: pFN21AE0920.seq Check: 5589 from: 1 to: 5644
pFN21AE0920 5644 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2046 2443 2046
Size: 397 5247
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2160 2595 2987 1937
Size: 223 435 392 4594
Fragments arranged by size:
4594 435 392 223
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2303 2752 2769 3707 5091 5359 1632
Size: 671 449 17 938 1384 268 1917
Fragments arranged by size:
1917 1384 938 671 449 268 17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2206 4543 5520 1203
Size: 1003 2337 977 1327
Fragments arranged by size:
2337 1327 1003 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 2566 4373 2566
Size: 1807 3837
AleI CACnn'nnGTG
Cuts at: 1166 2258 1166
Size: 1092 4552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2078 2276 1184
Size: 894 198 4552
Fragments arranged by size:
4552 894 198
AlwNI CAG_nnn'CTG
Cuts at: 2458 3387 4959 2458
Size: 929 1572 3143
Fragments arranged by size:
3143 1572 929
ApaI G_GGCC'C
Cuts at: 2129 2937 2129
Size: 808 4836
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2256 2296 2372 2616 2969 1870
Size: 73 313 40 76 244 353 4545
Fragments arranged by size:
4545 353 313 244 76 73 40
BamHI G'GATC_C
Cuts at: 2640 2691 2974 3341 2640
Size: 51 283 367 4943
Fragments arranged by size:
4943 367 283 51
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2965 4420 618
Size: 325 217 627 293 885 1455 1842
Fragments arranged by size:
1842 1455 885 627 325 293 217
BanII G_rGCy'C
Cuts at: 1391 2103 2129 2297 2419 2550 2937 2963 1391
Size: 712 26 168 122 131 387 26 4072
Fragments arranged by size:
4072 712 387 168 131 122 26 26
BbeI G_GCGC'C
Cuts at: 2084 2084
Size: 5644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3835 3869 1903
Size: 34 50 34 1814 34 3678
Fragments arranged by size:
3678 1814 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2386 5639 2386
Size: 3253 2391
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2265 2937 3705 4861 1790
Size: 339 136 672 768 1156 2573
Fragments arranged by size:
2573 1156 768 672 339 136
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2144 2242 3375
Size: 416 53 83 399 24 727 98 1133
Cuts at: 3375 4333 442
Size: 958 1753
Fragments arranged by size:
1753 1133 958 727 416 399 98 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2384 2480 2616 4304 1239
Size: 887 258 96 136 1688 2579
Fragments arranged by size:
2579 1688 887 258 136 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2534 2567 3769 4634 4932 2534
Size: 33 1202 865 298 3246
Fragments arranged by size:
3246 1202 865 298 33
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2785 2818 4307 882
Size: 617 1286 33 1489 2219
Fragments arranged by size:
2219 1489 1286 617 33
BsaAI yAC'GTr
Cuts at: 493 1204 2209 3435 493
Size: 711 1005 1226 2702
Fragments arranged by size:
2702 1226 1005 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 2310 3451 3833
Size: 53 83 186 936 548 229 1141 382
Cuts at: 3833 4468 275
Size: 635 1451
Fragments arranged by size:
1451 1141 936 635 548 382 229 186
83 53
BsaWI w'CCGG_w
Cuts at: 2220 4077 4475 4749 4896 5307 2220
Size: 1857 398 274 147 411 2557
Fragments arranged by size:
2557 1857 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 1178
Size: 30 165 30 696 30 4693
Fragments arranged by size:
4693 696 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 1186
Size: 1255 4389
BseYI C'CCAG_C
Cuts at: 2936 4847 5343 2936
Size: 1911 496 3237
Fragments arranged by size:
3237 1911 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 1163
Size: 573 312 4759
Fragments arranged by size:
4759 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2297 2496 2550 3705 3790 4861 2297
Size: 199 54 1155 85 1071 3080
Fragments arranged by size:
3080 1155 1071 199 85 54
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
BsmBI CGTCTCn'nnnn_
Cuts at: 2182 2182
Size: 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2129 2265 2297 2419 2496 2550
Size: 399 313 26 136 32 122 77 54
Cuts at: 2550 2937 2963 3705 3790 4861 1391
Size: 387 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 313 136 122
85 77 54 32 26 26
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2046 2070 2443 2655 2999 844
Size: 1202 24 373 212 344 3489
Fragments arranged by size:
3489 1202 373 344 212 24
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2191 2377 4288 4475 1700
Size: 110 381 186 1911 187 2869
Fragments arranged by size:
2869 1911 381 187 186 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 2041 1925
Size: 116 5528
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 2847 4448 2847
Size: 1601 4043
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2272 2077
Size: 195 5449
BstYI r'GATC_y
Cuts at: 1972 2386 2640 2691 2907 2974 3341 3726 3743
Size: 414 254 51 216 67 367 385 17
Cuts at: 3743 5195 5281 5293 5639 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 414 385 367 346 254 216
86 67 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2265 2659 2867 513
Size: 552 1200 394 208 3290
Fragments arranged by size:
3290 1200 552 394 208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2636 487
Size: 683 56 1410 3495
Fragments arranged by size:
3495 1410 683 56
BtsI GCAGTG_nn'
Cuts at: 2473 3160 3954 3974 2473
Size: 687 794 20 4143
Fragments arranged by size:
4143 794 687 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2351 4651 809
Size: 507 1035 2300 1802
Fragments arranged by size:
2300 1802 1035 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 3091 3980
Size: 54 1425 50 275 18 366 895 889
Cuts at: 3980 5609 8
Size: 1629 43
Fragments arranged by size:
1629 1425 895 889 366 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2396 2736 2788 3574 1353
Size: 1043 340 52 786 3423
Fragments arranged by size:
3423 1043 786 340 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2295 2548 2295
Size: 253 5391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2303 2384 2480 2616 2752 2769
Size: 393 494 177 81 96 136 136 17
Cuts at: 2769 3707 4304 5091 5359 1239
Size: 938 597 787 268 1524
Fragments arranged by size:
1524 938 787 597 494 393 268 177
136 136 96 81 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2423 2891 2423
Size: 468 5176
EcoO109I rG'GnC_Cy
Cuts at: 2613 2933 2613
Size: 320 5324
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2084 2579 4791 1678
Size: 406 495 2212 2531
Fragments arranged by size:
2531 2212 495 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 4285 4317 4359
Size: 32 135 32 980 32 1701 32 42
Cuts at: 4359 4391 1373
Size: 32 2626
Fragments arranged by size:
2626 1701 980 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KasI G'GCGC_C
Cuts at: 2080 2080
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2205 2253 4758 4942 1305
Size: 900 48 2505 184 2007
Fragments arranged by size:
2505 2007 900 184 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2198 10
Size: 54 1425 343 366 3456
Fragments arranged by size:
3456 1425 366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 2270 3602 3961 4120 518
Size: 648 1092 12 1332 359 159 2042
Fragments arranged by size:
2042 1332 1092 648 359 159 12
MspA1I CmG'CkG
Cuts at: 1606 2142 3060 3737 4885 5130 1606
Size: 536 918 677 1148 245 2120
Fragments arranged by size:
2120 1148 918 677 536 245
NarI GG'CG_CC
Cuts at: 2081 2081
Size: 5644
NcoI C'CATG_G
Cuts at: 513 1065 2265 2659 2867 513
Size: 552 1200 394 208 3290
Fragments arranged by size:
3290 1200 552 394 208
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 3002 4547 1132
Size: 97 1773 1545 2229
Fragments arranged by size:
2229 1773 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2686 1348
Size: 1338 4306
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PfoI T'CCnGG_A
Cuts at: 2322 2361 2322
Size: 39 5605
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PmlI CAC'GTG
Cuts at: 2209 2209
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2613 2613
Size: 5644
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PspOMI G'GGCC_C
Cuts at: 2125 2933 2125
Size: 808 4836
PspXI vC'TCGA_Gb
Cuts at: 2256 2256
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2996 830
Size: 2166 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
SacI G_AGCT'C
Cuts at: 2297 2550 2297
Size: 253 5391
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SanDI GG'GwC_CC
Cuts at: 2613 2613
Size: 5644
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SexAI A'CCwGG_T
Cuts at: 2118 2118
Size: 5644
SfcI C'TryA_G
Cuts at: 826 1045 2992 4127 4808 4999 826
Size: 219 1947 1135 681 191 1471
Fragments arranged by size:
1947 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2082 2082
Size: 5644
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2256 2549 2582 3748 4649 4911
Size: 197 926 313 293 33 1166 901 262
Cuts at: 4911 820
Size: 1553
Fragments arranged by size:
1553 1166 926 901 313 293 262 197
33
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2265 2659 2867 3083 513
Size: 552 1200 394 208 216 3074
Fragments arranged by size:
3074 1200 552 394 216 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2146 3671 3688 3841 4026 4471 325
Size: 1255 566 1525 17 153 185 445 1498
Fragments arranged by size:
1525 1498 1255 566 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3890 5602 96
Size: 274 80 33 51 1443 1913 1712 138
Fragments arranged by size:
1913 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 2591 1316
Size: 1275 4369
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2206 2657 2206
Size: 451 5193
XhoI C'TCGA_G
Cuts at: 1943 2256 1943
Size: 313 5331
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SacI SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PsrI PvuII
RsrII SacII SapI SfiI SrfI SwaI