Restriction map for
pFN21AE0914
(Circular) (Six-base) MAPSORT of: pFN21AE0914.seq Check: 3115 from: 1 to: 5476
pFN21AE0914 5476 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3286 278
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2819 1937
Size: 882 4594
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 2016 3603 3976 1563
Size: 453 1587 373 3063
Fragments arranged by size:
3063 1587 453 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3539 4923 5191 1632
Size: 1907 1384 268 1917
Fragments arranged by size:
1917 1907 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 4375 5352 1203
Size: 3172 977 1327
Fragments arranged by size:
3172 1327 977
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 4205 4205
Size: 5476
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5476
AlwNI CAG_nnn'CTG
Cuts at: 2514 2545 3219 4791 2514
Size: 31 674 1572 3199
Fragments arranged by size:
3199 1572 674 31
ApaLI G'TGCA_C
Cuts at: 3533 4689 3533
Size: 1156 4320
ApoI r'AATT_y
Cuts at: 1493 2159 2785 3011 3163 5272 1493
Size: 666 626 226 152 2109 1697
Fragments arranged by size:
2109 1697 666 626 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 2801 1870
Size: 73 765 93 4545
Fragments arranged by size:
4545 765 93 73
BamHI G'GATC_C
Cuts at: 2806 3173 2806
Size: 367 5109
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2134 2797 4252 618
Size: 325 217 627 347 663 1455 1842
Fragments arranged by size:
1842 1455 663 627 347 325 217
BanII G_rGCy'C
Cuts at: 1391 2211 2715 2795 1391
Size: 820 504 80 4072
Fragments arranged by size:
4072 820 504 80
BbeI G_GCGC'C
Cuts at: 2138 2138
Size: 5476
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2578 928
Size: 971 679 3826
Fragments arranged by size:
3826 971 679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3667 3701 1903
Size: 34 50 34 1646 34 3678
Fragments arranged by size:
3678 1646 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4087 136
Size: 107 122 71 631 605 2415 1525
Fragments arranged by size:
2415 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2893 2893
Size: 5476
Bme1580I G_kGCm'C
Cuts at: 1790 3537 4693 1790
Size: 1747 1156 2573
Fragments arranged by size:
2573 1747 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3207 4165 442
Size: 416 53 83 399 24 1790 958 1753
Fragments arranged by size:
1790 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2321 2509 2626 2781 4136 1239
Size: 1082 188 117 155 1355 2579
Fragments arranged by size:
2579 1355 1082 188 155 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3601 4466 4764 3601
Size: 865 298 4313
Fragments arranged by size:
4313 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2363 4139 882
Size: 617 864 1776 2219
Fragments arranged by size:
2219 1776 864 617
BsaAI yAC'GTr
Cuts at: 493 1204 3267 493
Size: 711 2063 2702
Fragments arranged by size:
2702 2063 711
BsaBI GATnn'nnATC
Cuts at: 5470 5470
Size: 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2135 3283 3665 4300
Size: 53 83 186 936 602 1148 382 635
Cuts at: 4300 275
Size: 1451
Fragments arranged by size:
1451 1148 936 635 602 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3909 4307 4581 4728 5139 3909
Size: 398 274 147 411 4246
Fragments arranged by size:
4246 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2319 2349 1178
Size: 30 165 30 916 30 4305
Fragments arranged by size:
4305 916 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2102 2340 2668 2744 1186
Size: 913 3 238 328 76 3918
Fragments arranged by size:
3918 913 328 238 76 3
BseYI C'CCAG_C
Cuts at: 2138 2320 4679 5175 2138
Size: 182 2359 496 2439
Fragments arranged by size:
2439 2359 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 1163
Size: 573 345 4558
Fragments arranged by size:
4558 573 345
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2292 2424 2926 3687 4715 1540
Size: 275 186 291 132 502 761 1028 2301
Fragments arranged by size:
2301 1028 761 502 291 275 186 132
BsiHKAI G_wGCw'C
Cuts at: 2724 3537 3622 4693 2724
Size: 813 85 1071 3507
Fragments arranged by size:
3507 1071 813 85
BsmI GAATG_Cn'
Cuts at: 2987 3080 2987
Size: 93 5383
BsmBI CGTCTCn'nnnn_
Cuts at: 2443 2443
Size: 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2715 2724 2795 3537 3622 4693
Size: 399 421 504 9 71 742 85 1071
Cuts at: 4693 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 504 421 399 85 71
9
BspEI T'CCGG_A
Cuts at: 5139 5139
Size: 5476
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2544 2831 844
Size: 1700 287 3489
Fragments arranged by size:
3489 1700 287
BsrBI CCG'CTC
Cuts at: 1394 1823 2202 2412 3363 1394
Size: 429 379 210 951 3507
Fragments arranged by size:
3507 951 429 379 210
BsrDI GCAATG_nn'
Cuts at: 65 1248 3971 4145 65
Size: 1183 2723 174 1396
Fragments arranged by size:
2723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4120 4307 1700
Size: 110 2310 187 2869
Fragments arranged by size:
2869 2310 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5476
BssHII G'CGCG_C
Cuts at: 1925 2468 1925
Size: 543 4933
BssSI C'ACGA_G
Cuts at: 3536 4548 3536
Size: 1012 4464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5476
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 2308 1184
Size: 1124 4352
BstYI r'GATC_y
Cuts at: 1972 2542 2806 3173 3558 3575 5027 5113 5125
Size: 570 264 367 385 17 1452 86 12
Cuts at: 5125 5471 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 570 385 367 346 264 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2286 2500 513
Size: 552 1221 214 3489
Fragments arranged by size:
3489 1221 552 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4737
Fragments arranged by size:
4737 683 56
BtsI GCAGTG_nn'
Cuts at: 2992 3786 3806 2992
Size: 794 20 4662
Fragments arranged by size:
4662 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2780 2780
Size: 5476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4483 809
Size: 507 3167 1802
Fragments arranged by size:
3167 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2289 2421 2503
Size: 54 1425 50 275 18 459 132 82
Cuts at: 2503 2923 3812 5441 8
Size: 420 889 1629 43
Fragments arranged by size:
1629 1425 889 459 420 275 132 82
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2289 2421 2923 1537
Size: 275 477 132 502 4090
Fragments arranged by size:
4090 502 477 275 132
EarI CTCTTCn'nnn_
Cuts at: 1353 2767 3406 1353
Size: 1414 639 3423
Fragments arranged by size:
3423 1414 639
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4063 4437 4583 387
Size: 1064 138 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2509 2626 2781 3539 4136 4923
Size: 393 689 188 117 155 758 597 787
Cuts at: 4923 5191 1239
Size: 268 1524
Fragments arranged by size:
1524 787 758 689 597 393 268 188
155 117
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
EcoRV GAT'ATC
Cuts at: 2149 2149
Size: 5476
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 2138 2553 2649 4623 1678
Size: 460 415 96 1974 2531
Fragments arranged by size:
2531 1974 460 415 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2597 2629 4117
Size: 32 135 32 467 32 526 32 1488
Cuts at: 4117 4149 4191 4223 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1488 526 467 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2820 3066 669
Size: 988 281 882 246 3079
Fragments arranged by size:
3079 988 882 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KasI G'GCGC_C
Cuts at: 2134 2134
Size: 5476
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4590 4774 1305
Size: 3285 184 2007
Fragments arranged by size:
3285 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3654
Fragments arranged by size:
3654 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3434 3793 3952 518
Size: 648 2268 359 159 2042
Fragments arranged by size:
2268 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2299 2481 2502 2892 3569 4717 4962 1606
Size: 693 182 21 390 677 1148 245 2120
Fragments arranged by size:
2120 1148 693 677 390 245 182 21
NarI GG'CG_CC
Cuts at: 2135 2135
Size: 5476
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4924
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2834 4379 1132
Size: 97 1605 1545 2229
Fragments arranged by size:
2229 1605 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5476
PciI A'CATG_T
Cuts at: 4375 4375
Size: 5476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5476
PfoI T'CCnGG_A
Cuts at: 2394 2394
Size: 5476
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5476
PsiI TTA'TAA
Cuts at: 3046 3046
Size: 5476
PstI C_TGCA'G
Cuts at: 830 2127 2541 2828 830
Size: 1297 414 287 3478
Fragments arranged by size:
3478 1297 414 287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
PvuII CAG'CTG
Cuts at: 2299 2299
Size: 5476
SacII CC_GC'GG
Cuts at: 2503 2503
Size: 5476
SalI G'TCGA_C
Cuts at: 1936 2818 1936
Size: 882 4594
SbfI CC_TGCA'GG
Cuts at: 2541 2828 2541
Size: 287 5189
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2123 2537 2824 3959 4640 4831 826
Size: 219 1078 414 287 1135 681 191 1471
Fragments arranged by size:
1471 1135 1078 681 414 287 219 191
SfoI GGC'GCC
Cuts at: 2136 2136
Size: 5476
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2803 1872
Size: 931 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3580 4481 4743 820
Size: 197 926 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 3400 5
Size: 47 3348 2081
Fragments arranged by size:
3348 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5476
StyI C'CwwG_G
Cuts at: 513 1065 2915 513
Size: 552 1850 3074
Fragments arranged by size:
3074 1850 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2327 3503 3520 3673 3858 4303 325
Size: 1255 747 1176 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1176 747 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3722 5434 96
Size: 274 80 33 51 1443 1745 1712 138
Fragments arranged by size:
1745 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2319 2351 2390 2422 5436 5468 2319
Size: 32 39 32 3014 32 2327
Fragments arranged by size:
3014 2327 39 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2686 1316
Size: 1370 4106
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5476
XmaI C'CCGG_G
Cuts at: 1870 2801 1870
Size: 931 4545
XmnI GAAnn'nnTTC
Cuts at: 2159 3605 2159
Size: 1446 4030
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3284 276
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI
XcmI