Restriction map for
pFN21AE0912
(Circular) (Six-base) MAPSORT of: pFN21AE0912.seq Check: 5861 from: 1 to: 5443
pFN21AE0912 5443 bp
With 182 enzymes: SgfI *
March 28, 2012 12:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2506 2506
Size: 5443
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 1937
Size: 849 4594
Acc65I G'GTAC_C
Cuts at: 2442 2764 2442
Size: 322 5121
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2685 3506 4890 5158 1632
Size: 1053 821 1384 268 1917
Fragments arranged by size:
1917 1384 1053 821 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 4342 5319 1203
Size: 3139 977 1327
Fragments arranged by size:
3139 1327 977
AgeI A'CCGG_T
Cuts at: 4274 4274
Size: 5443
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5443
AlwNI CAG_nnn'CTG
Cuts at: 2272 2593 3186 4758 2272
Size: 321 593 1572 2957
Fragments arranged by size:
2957 1572 593 321
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2026 2752 2978 3130 5239 1493
Size: 533 726 226 152 2109 1697
Fragments arranged by size:
2109 1697 726 533 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2768 1870
Size: 73 825 4545
Fragments arranged by size:
4545 825 73
BamHI G'GATC_C
Cuts at: 2452 2773 3140 2452
Size: 321 367 4755
Fragments arranged by size:
4755 367 321
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2442 2764 4219 618
Size: 325 217 627 655 322 1455 1842
Fragments arranged by size:
1842 1455 655 627 325 322 217
BanII G_rGCy'C
Cuts at: 1391 2214 2487 2762 1391
Size: 823 273 275 4072
Fragments arranged by size:
4072 823 275 273
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2323 2402 928
Size: 971 424 79 3969
Fragments arranged by size:
3969 971 424 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3634 3668 1903
Size: 34 1697 34 3678
Fragments arranged by size:
3678 1697 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2129 2138 2183
Size: 107 122 71 631 605 457 9 45
Cuts at: 2183 4054 136
Size: 1871 1525
Fragments arranged by size:
1871 1525 631 605 457 122 107 71
45 9
BglII A'GATC_T
Cuts at: 5438 5438
Size: 5443
BlpI GC'TnA_GC
Cuts at: 2860 2860
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1790 2617 3504 4660 1790
Size: 827 887 1156 2573
Fragments arranged by size:
2573 1156 887 827
BmgBI CAC'GTC
Cuts at: 1318 2718 1318
Size: 1400 4043
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3174 4132 442
Size: 416 53 83 399 24 1757 958 1753
Fragments arranged by size:
1757 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2564 4103 1239
Size: 1325 1539 2579
Fragments arranged by size:
2579 1539 1325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3568 4433 4731 3568
Size: 865 298 4280
Fragments arranged by size:
4280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 4106 882
Size: 617 555 2052 2219
Fragments arranged by size:
2219 2052 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 3234 493
Size: 711 2030 2702
Fragments arranged by size:
2702 2030 711
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2655 3250 3632 4267
Size: 53 83 186 936 1122 595 382 635
Cuts at: 4267 275
Size: 1451
Fragments arranged by size:
1451 1122 936 635 595 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2498 3876 4274 4548 4695 5106 2498
Size: 1378 398 274 147 411 2835
Fragments arranged by size:
2835 1378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5218
Fragments arranged by size:
5218 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5443
BseYI C'CCAG_C
Cuts at: 2037 2468 2650 4646 5142 2037
Size: 431 182 1996 496 2338
Fragments arranged by size:
2338 1996 496 431 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4870
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2673 2893 3654 4682 1540
Size: 275 186 672 220 761 1028 2301
Fragments arranged by size:
2301 1028 761 672 275 220 186
BsiHKAI G_wGCw'C
Cuts at: 2214 2487 3504 3589 4660 2214
Size: 273 1017 85 1071 2997
Fragments arranged by size:
2997 1071 1017 273 85
BsmI GAATG_Cn'
Cuts at: 2954 3047 2954
Size: 93 5350
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2487 2617 2762 3504 3589 4660
Size: 399 424 273 130 145 742 85 1071
Cuts at: 4660 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 424 399 273 145 130
85
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 3332 5062 3332
Size: 1730 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2436 2454 2506 2578 2798 844
Size: 1592 18 52 72 220 3489
Fragments arranged by size:
3489 1592 220 72 52 18
BsrBI CCG'CTC
Cuts at: 1394 1823 2076 2558 3330 1394
Size: 429 253 482 772 3507
Fragments arranged by size:
3507 772 482 429 253
BsrDI GCAATG_nn'
Cuts at: 65 1248 3938 4112 65
Size: 1183 2690 174 1396
Fragments arranged by size:
2690 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4087 4274 1700
Size: 110 2277 187 2869
Fragments arranged by size:
2869 2277 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 2172 3503 4515 2172
Size: 1331 1012 3100
Fragments arranged by size:
3100 1331 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5443
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2452 2773 3140 3525 3542 4994 5080 5092
Size: 480 321 367 385 17 1452 86 12
Cuts at: 5092 5438 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 480 385 367 346 321 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2118 513
Size: 552 1053 3838
Fragments arranged by size:
3838 1053 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2094 487
Size: 683 56 868 3836
Fragments arranged by size:
3836 868 683 56
BtsI GCAGTG_nn'
Cuts at: 2401 2959 3753 3773 2401
Size: 558 794 20 4071
Fragments arranged by size:
4071 794 558 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2747 2747
Size: 5443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4450 809
Size: 507 3134 1802
Fragments arranged by size:
3134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2670 2694
Size: 54 1425 50 275 18 216 624 24
Cuts at: 2694 2890 3779 5408 8
Size: 196 889 1629 43
Fragments arranged by size:
1629 1425 889 624 275 216 196 54
50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2670 2890 1537
Size: 275 858 220 4090
Fragments arranged by size:
4090 858 275 220
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 3373 1353
Size: 711 1309 3423
Fragments arranged by size:
3423 1309 711
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2639 4030 4404 4550 387
Size: 1064 138 1050 1391 374 146 1280
Fragments arranged by size:
1391 1280 1064 1050 374 146 138
EcoICRI GAG'CTC
Cuts at: 2212 2485 2212
Size: 273 5170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2564 2685 3506 4103 4890 5158 1239
Size: 393 932 121 821 597 787 268 1524
Fragments arranged by size:
1524 932 821 787 597 393 268 121
EcoNI CCTnn'n_nnAGG
Cuts at: 2597 2597
Size: 5443
EcoO109I rG'GnC_Cy
Cuts at: 2140 2327 2590 2140
Size: 187 263 4993
Fragments arranged by size:
4993 263 187
EcoRI G'AATT_C
Cuts at: 2752 3130 2752
Size: 378 5065
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 2092 2273 4590 1678
Size: 414 181 2317 2531
Fragments arranged by size:
2531 2317 414 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4084 4116 4158 4190 1373
Size: 32 135 32 2512 32 42 32 2626
Fragments arranged by size:
2626 2512 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2787 3033 669
Size: 988 281 849 246 3079
Fragments arranged by size:
3079 988 849 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KpnI G_GTAC'C
Cuts at: 2446 2768 2446
Size: 322 5121
MfeI C'AATT_G
Cuts at: 3042 3042
Size: 5443
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4557 4741 1305
Size: 3252 184 2007
Fragments arranged by size:
3252 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3621
Fragments arranged by size:
3621 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3401 3760 3919 518
Size: 648 2235 359 159 2042
Fragments arranged by size:
2235 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2365 2859 3536 4684 4929 1606
Size: 408 351 494 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 494 408 351 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4891
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2801 4346 1132
Size: 97 1572 1545 2229
Fragments arranged by size:
2229 1572 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2323 1348
Size: 975 4468
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2044 1108
Size: 936 4507
PfoI T'CCnGG_A
Cuts at: 2223 2223
Size: 5443
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2327 2590 2327
Size: 263 5180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 3013 3013
Size: 5443
PstI C_TGCA'G
Cuts at: 830 2268 2451 2566 2795 830
Size: 1438 183 115 229 3478
Fragments arranged by size:
3478 1438 229 183 115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
PvuII CAG'CTG
Cuts at: 2014 2014
Size: 5443
SacI G_AGCT'C
Cuts at: 2214 2487 2214
Size: 273 5170
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SbfI CC_TGCA'GG
Cuts at: 2451 2795 2451
Size: 344 5099
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SfcI C'TryA_G
Cuts at: 826 1045 2264 2447 2562 2791 3926 4607 4798
Size: 219 1219 183 115 229 1135 681 191
Cuts at: 4798 826
Size: 1471
Fragments arranged by size:
1471 1219 1135 681 229 219 191 183
115
SfiI GGCCn_nnn'nGGCC
Cuts at: 2129 2138 2129
Size: 9 5434
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2770 1872
Size: 898 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3547 4448 4710 820
Size: 197 926 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2801 1132
Size: 1669 3774
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5443
StyI C'CwwG_G
Cuts at: 513 1065 2244 2882 513
Size: 552 1179 638 3074
Fragments arranged by size:
3074 1179 638 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2568 3470 3487 3640 3825 4270 325
Size: 1255 988 902 17 153 185 445 1498
Fragments arranged by size:
1498 1255 988 902 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3689 5401 96
Size: 274 80 33 51 1443 1712 1712 138
Fragments arranged by size:
1712 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5403 5435 5403
Size: 32 5411
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2768 1870
Size: 898 4545
XmnI GAAnn'nnTTC
Cuts at: 2357 3572 2357
Size: 1215 4228
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI
XcmI