Restriction map for
pFN21AE0909
(Circular) (Six-base) MAPSORT of: pFN21AE0909.seq Check: 8228 from: 1 to: 5380
pFN21AE0909 5380 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2269 2269
Size: 5380
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3190 278
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2496 2723 1937
Size: 559 227 4594
Fragments arranged by size:
4594 559 227
Acc65I G'GTAC_C
Cuts at: 2701 2701
Size: 5380
AclI AA'CG_TT
Cuts at: 1563 3507 3880 1563
Size: 1944 373 3063
Fragments arranged by size:
3063 1944 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 2399 3443 4827 5095 1632
Size: 746 21 1044 1384 268 1917
Fragments arranged by size:
1917 1384 1044 746 268 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5183
AflIII A'CryG_T
Cuts at: 1203 2019 4279 5256 1203
Size: 816 2260 977 1327
Fragments arranged by size:
2260 1327 977 816
AgeI A'CCGG_T
Cuts at: 4211 4211
Size: 5380
AhdI GACnn_n'nnGTC
Cuts at: 4109 4109
Size: 5380
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5380
AlwNI CAG_nnn'CTG
Cuts at: 2044 3123 4695 2044
Size: 1079 1572 2729
Fragments arranged by size:
2729 1572 1079
ApaI G_GGCC'C
Cuts at: 2241 2322 2588 2241
Size: 81 266 5033
Fragments arranged by size:
5033 266 81
ApaLI G'TGCA_C
Cuts at: 3437 4593 3437
Size: 1156 4224
ApoI r'AATT_y
Cuts at: 1493 2689 2915 3067 5176 1493
Size: 1196 226 152 2109 1697
Fragments arranged by size:
2109 1697 1196 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3934 160
Size: 872 2902 1606
Fragments arranged by size:
2902 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2141 2518 2705 1870
Size: 73 130 68 377 187 4545
Fragments arranged by size:
4545 377 187 130 73 68
BamHI G'GATC_C
Cuts at: 2710 3077 2710
Size: 367 5013
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2152 2701 4156 618
Size: 325 217 627 365 549 1455 1842
Fragments arranged by size:
1842 1455 627 549 365 325 217
BanII G_rGCy'C
Cuts at: 1391 2241 2322 2588 2699 1391
Size: 850 81 266 111 4072
Fragments arranged by size:
4072 850 266 111 81
BbeI G_GCGC'C
Cuts at: 2156 2156
Size: 5380
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3571 3605 1903
Size: 34 50 34 1550 34 3678
Fragments arranged by size:
3678 1550 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3272 4482 3272
Size: 1210 4170
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5380
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3991 136
Size: 107 122 71 631 605 2319 1525
Fragments arranged by size:
2319 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2406 5375 2406
Size: 2969 2411
BlpI GC'TnA_GC
Cuts at: 2797 2797
Size: 5380
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2241 2322 2588 3441 4597 1790
Size: 352 99 81 266 853 1156 2573
Fragments arranged by size:
2573 1156 853 352 266 99 81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5380
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3111 4069 442
Size: 416 53 83 399 24 1694 958 1753
Fragments arranged by size:
1753 1694 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 4040 1239
Size: 827 1974 2579
Fragments arranged by size:
2579 1974 827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2429 3505 4370 4668 2429
Size: 1076 865 298 3141
Fragments arranged by size:
3141 1076 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2657 4043 882
Size: 617 1158 1386 2219
Fragments arranged by size:
2219 1386 1158 617
BsaAI yAC'GTr
Cuts at: 493 1204 3171 493
Size: 711 1967 2702
Fragments arranged by size:
2702 1967 711
BsaBI GATnn'nnATC
Cuts at: 5374 5374
Size: 5380
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2153 3187 3569 4204
Size: 53 83 186 936 620 1034 382 635
Cuts at: 4204 275
Size: 1451
Fragments arranged by size:
1451 1034 936 635 620 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2548 3813 4211 4485 4632 5043 2548
Size: 1265 398 274 147 411 2885
Fragments arranged by size:
2885 1265 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2522 2552 1178
Size: 30 165 30 1119 30 4006
Fragments arranged by size:
4006 1119 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5380
BseYI C'CCAG_C
Cuts at: 2008 2029 2233 4583 5079 2008
Size: 21 204 2350 496 2309
Fragments arranged by size:
2350 2309 496 204 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 2438 1163
Size: 573 561 141 4105
Fragments arranged by size:
4105 573 561 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2253 2830 3591 4619 1540
Size: 275 186 252 577 761 1028 2301
Fragments arranged by size:
2301 1028 761 577 275 252 186
BsiHKAI G_wGCw'C
Cuts at: 3441 3526 4597 3441
Size: 85 1071 4224
Fragments arranged by size:
4224 1071 85
BsmI GAATG_Cn'
Cuts at: 2891 2984 2891
Size: 93 5287
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2241 2322 2588 2699 3441 3526
Size: 399 352 99 81 266 111 742 85
Cuts at: 3526 4597 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 352 266 111 99
85 81
BspEI T'CCGG_A
Cuts at: 5043 5043
Size: 5380
BspHI T'CATG_A
Cuts at: 3269 4999 3269
Size: 1730 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2269 2735 844
Size: 1425 466 3489
Fragments arranged by size:
3489 1425 466
BsrBI CCG'CTC
Cuts at: 1394 1823 3267 1394
Size: 429 1444 3507
Fragments arranged by size:
3507 1444 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3875 4049 65
Size: 1183 2627 174 1396
Fragments arranged by size:
2627 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 2248 4024 4211 1700
Size: 110 297 141 1776 187 2869
Fragments arranged by size:
2869 1776 297 187 141 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5380
BssSI C'ACGA_G
Cuts at: 3440 4452 3440
Size: 1012 4368
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5380
BstBI TT'CG_AA
Cuts at: 4184 4184
Size: 5380
BstEII G'GTnAC_C
Cuts at: 1184 2281 1184
Size: 1097 4283
BstYI r'GATC_y
Cuts at: 1972 2178 2332 2406 2710 3077 3462 3479 4931
Size: 206 154 74 304 367 385 17 1452
Cuts at: 4931 5017 5029 5375 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 304 206 154
86 74 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2253 513
Size: 552 1188 3640
Fragments arranged by size:
3640 1188 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4641
Fragments arranged by size:
4641 683 56
BtsI GCAGTG_nn'
Cuts at: 2118 2896 3690 3710 2118
Size: 778 794 20 3788
Fragments arranged by size:
3788 794 778 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2080 2115 529
Size: 35 1516 35 3794
Fragments arranged by size:
3794 1516 35 35
DraI TTT'AAA
Cuts at: 2684 2684
Size: 5380
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2299 2492 4387 809
Size: 507 983 193 1895 1802
Fragments arranged by size:
1895 1802 983 507 193
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2250 2827
Size: 54 1425 50 275 18 381 39 577
Cuts at: 2827 3716 5345 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 577 381 275 54 50
43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2250 2827 1537
Size: 275 438 577 4090
Fragments arranged by size:
4090 577 438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2159 2612 3310 1353
Size: 806 453 698 3423
Fragments arranged by size:
3423 806 698 453
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3967 4341 4487 387
Size: 1064 138 2378 374 146 1280
Fragments arranged by size:
2378 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2378 2399 3443 4040 4827 5095
Size: 393 434 312 21 1044 597 787 268
Cuts at: 5095 1239
Size: 1524
Fragments arranged by size:
1524 1044 787 597 434 393 312 268
21
EcoO109I rG'GnC_Cy
Cuts at: 2237 2238 2318 2344 2473 2584 2585 2237
Size: 1 80 26 129 111 1 5032
Fragments arranged by size:
5032 129 111 80 26 1 1
EcoRI G'AATT_C
Cuts at: 2689 3067 2689
Size: 378 5002
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2428 2188
Size: 240 5140
FspI TGC'GCA
Cuts at: 3886 3886
Size: 5380
HaeII r_GCGC'y
Cuts at: 1678 2066 2156 2526 2564 4527 1678
Size: 388 90 370 38 1963 2531
Fragments arranged by size:
2531 1963 388 370 90 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2391 2423 4021 4053 4095
Size: 32 135 32 819 32 1598 32 42
Cuts at: 4095 4127 1373
Size: 32 2626
Fragments arranged by size:
2626 1598 819 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2724 2970 669
Size: 988 281 786 246 3079
Fragments arranged by size:
3079 988 786 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5380
HpaI GTT'AAC
Cuts at: 2970 2970
Size: 5380
KasI G'GCGC_C
Cuts at: 2152 2152
Size: 5380
KpnI G_GTAC'C
Cuts at: 2705 2705
Size: 5380
MfeI C'AATT_G
Cuts at: 2979 2979
Size: 5380
MluI A'CGCG_T
Cuts at: 2019 5256 2019
Size: 3237 2143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2106 2283 4494 4678 1305
Size: 801 177 2211 184 2007
Fragments arranged by size:
2211 2007 801 184 177
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 10
Size: 54 1425 343 381 3177
Fragments arranged by size:
3177 1425 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3338 3697 3856 518
Size: 648 2172 359 159 2042
Fragments arranged by size:
2172 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2188 2439 2796 3473 4621 4866 1606
Size: 435 147 251 357 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 435 357 251 245 147
NaeI GCC'GGC
Cuts at: 2109 2109
Size: 5380
NarI GG'CG_CC
Cuts at: 2153 2153
Size: 5380
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4828
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5380
NgoMIV G'CCGG_C
Cuts at: 2107 2107
Size: 5380
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5380
NotI GC'GGCC_GC
Cuts at: 2827 2827
Size: 5380
NspI r_CATG'y
Cuts at: 1132 1229 2738 4283 1132
Size: 97 1509 1545 2229
Fragments arranged by size:
2229 1545 1509 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5380
PciI A'CATG_T
Cuts at: 4279 4279
Size: 5380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5380
PmeI GTTT'AAAC
Cuts at: 2684 2684
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 4962 4994 3449
Size: 32 1481 32 3835
Fragments arranged by size:
3835 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2344 2344
Size: 5380
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5380
PsiI TTA'TAA
Cuts at: 2950 2950
Size: 5380
PspOMI G'GGCC_C
Cuts at: 2237 2318 2584 2237
Size: 81 266 5033
Fragments arranged by size:
5033 266 81
PstI C_TGCA'G
Cuts at: 830 2732 830
Size: 1902 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5380
PvuII CAG'CTG
Cuts at: 2041 2188 2041
Size: 147 5233
SalI G'TCGA_C
Cuts at: 1936 2722 1936
Size: 786 4594
SapI GCTCTTCn'nnn_
Cuts at: 2159 2159
Size: 5380
SbfI CC_TGCA'GG
Cuts at: 2732 2732
Size: 5380
ScaI AGT'ACT
Cuts at: 3628 5340 3628
Size: 1712 3668
SexAI A'CCwGG_T
Cuts at: 2364 2364
Size: 5380
SfcI C'TryA_G
Cuts at: 826 1045 2728 3863 4544 4735 826
Size: 219 1683 1135 681 191 1471
Fragments arranged by size:
1683 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2154 2154
Size: 5380
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5380
SmaI CCC'GGG
Cuts at: 1872 2707 1872
Size: 835 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 3484 4385 4647 820
Size: 197 926 501 1040 901 262 1553
Fragments arranged by size:
1553 1040 926 901 501 262 197
SnaBI TAC'GTA
Cuts at: 493 3171 493
Size: 2678 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5380
SphI G_CATG'C
Cuts at: 1132 2738 1132
Size: 1606 3774
SspI AAT'ATT
Cuts at: 5 52 3304 5
Size: 47 3252 2081
Fragments arranged by size:
3252 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5380
StyI C'CwwG_G
Cuts at: 513 1065 2819 513
Size: 552 1754 3074
Fragments arranged by size:
3074 1754 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3407 3424 3577 3762 4207 325
Size: 1255 1827 17 153 185 445 1498
Fragments arranged by size:
1827 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2217 3626 5338
Size: 274 80 33 51 1443 240 1409 1712
Cuts at: 5338 96
Size: 138
Fragments arranged by size:
1712 1443 1409 274 240 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2621 2653 5340 5372 2621
Size: 32 2687 32 2629
Fragments arranged by size:
2687 2629 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5380
XbaI T'CTAG_A
Cuts at: 2716 2716
Size: 5380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5380
XmaI C'CCGG_G
Cuts at: 1870 2705 1870
Size: 835 4545
XmnI GAAnn'nnTTC
Cuts at: 3509 3509
Size: 5380
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3188 276
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI
SrfI SwaI