Restriction map for
pFN21AE0904
(Circular) (Six-base) MAPSORT of: pFN21AE0904.seq Check: 8927 from: 1 to: 5776
pFN21AE0904 5776 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2219 3119 1937
Size: 282 900 4594
Fragments arranged by size:
4594 900 282
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2289 2325 2342 2859 3839 5223 5491 1632
Size: 657 36 17 517 980 1384 268 1917
Fragments arranged by size:
1917 1384 980 657 517 268 36 17
AfeI AGC'GCT
Cuts at: 2037 2621 2037
Size: 584 5192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 2967 4675 5652 1203
Size: 1764 1708 977 1327
Fragments arranged by size:
1764 1708 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 2095 1166
Size: 929 4847
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2758 2790 2758
Size: 32 5744
AlwNI CAG_nnn'CTG
Cuts at: 3519 5091 3519
Size: 1572 4204
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 3085 3311 3463 5572 1493
Size: 1592 226 152 2109 1697
Fragments arranged by size:
2109 1697 1592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2206 3101 1870
Size: 73 96 167 895 4545
Fragments arranged by size:
4545 895 167 96 73
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2184 3097 4552 618
Size: 325 217 627 397 913 1455 1842
Fragments arranged by size:
1842 1455 913 627 397 325 217
BanII G_rGCy'C
Cuts at: 1391 2533 2643 2810 3095 1391
Size: 1142 110 167 285 4072
Fragments arranged by size:
4072 1142 285 167 110
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2111 2586 928
Size: 971 212 475 4118
Fragments arranged by size:
4118 971 475 212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3036 4387 136
Size: 107 122 71 631 605 1364 1351 1525
Fragments arranged by size:
1525 1364 1351 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 2642 3193 2642
Size: 551 5225
Bme1580I G_kGCm'C
Cuts at: 1790 3837 4993 1790
Size: 2047 1156 2573
Fragments arranged by size:
2573 2047 1156
BmgBI CAC'GTC
Cuts at: 1318 2955 2970 1318
Size: 1637 15 4124
Fragments arranged by size:
4124 1637 15
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2151 3507 4465
Size: 416 53 83 399 24 734 1356 958
Cuts at: 4465 442
Size: 1753
Fragments arranged by size:
1753 1356 958 734 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 4436 1239
Size: 1073 2124 2579
Fragments arranged by size:
2579 2124 1073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2679 3901 4766 5064 2679
Size: 1222 865 298 3391
Fragments arranged by size:
3391 1222 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2927 3567 493
Size: 711 1723 640 2702
Fragments arranged by size:
2702 1723 711 640
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2565 3028 3583 3965
Size: 53 83 186 936 1032 463 555 382
Cuts at: 3965 4600 275
Size: 635 1451
Fragments arranged by size:
1451 1032 936 635 555 463 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2758 4209 4607 4881 5028 5439 2758
Size: 1451 398 274 147 411 3095
Fragments arranged by size:
3095 1451 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2696 2726 1178
Size: 30 165 30 1293 30 4228
Fragments arranged by size:
4228 1293 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2423 2569 2693 2901 1186
Size: 1237 146 124 208 4061
Fragments arranged by size:
4061 1237 208 146 124
BseYI C'CCAG_C
Cuts at: 4979 5475 4979
Size: 496 5280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2121 2243 1163
Size: 573 385 122 4696
Fragments arranged by size:
4696 573 385 122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2636 2919 3226 3987 5015 1540
Size: 275 186 635 283 307 761 1028 2301
Fragments arranged by size:
2301 1028 761 635 307 283 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 2351 3837 3922 4993 2073
Size: 278 1486 85 1071 2856
Fragments arranged by size:
2856 1486 1071 278 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
BsmBI CGTCTCn'nnnn_
Cuts at: 2245 2245
Size: 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2351 2533 2643 2810 3095 3837
Size: 399 283 278 182 110 167 285 742
Cuts at: 3837 3922 4993 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 285 283 278 182
167 110 85
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3131 844
Size: 2287 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2272 3106 3473 3858 3875 5327 5413 5425
Size: 300 834 367 385 17 1452 86 12
Cuts at: 5425 5771 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 834 385 367 346 300 86
17 12
BstZ17I GTA'TAC
Cuts at: 2220 2220
Size: 5776
BtgI C'CryG_G
Cuts at: 513 1065 2004 2371 2931 2980 513
Size: 552 939 367 560 49 3309
Fragments arranged by size:
3309 939 560 552 367 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2071 3040 3292 4086 4106 2071
Size: 969 252 794 20 3741
Fragments arranged by size:
3741 969 794 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2809 2844 529
Size: 35 2245 35 3461
Fragments arranged by size:
3461 2245 35 35
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2670 2916 3037
Size: 54 1425 50 275 18 840 246 121
Cuts at: 3037 3223 4112 5741 8
Size: 186 889 1629 43
Fragments arranged by size:
1629 1425 889 840 275 246 186 121
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2916 3223 1537
Size: 275 1104 307 4090
Fragments arranged by size:
4090 1104 307 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2174 2243 2472 2484 2819 2835 3000 3706
Size: 821 69 229 12 335 16 165 706
Cuts at: 3706 1353
Size: 3423
Fragments arranged by size:
3423 821 706 335 229 165 69 16
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2289 2312 2325 2342 2859 3839 4436
Size: 393 657 23 13 17 517 980 597
Cuts at: 4436 5223 5491 1239
Size: 787 268 1524
Fragments arranged by size:
1524 980 787 657 597 517 393 268
23 17 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 2324
Size: 5776
EcoO109I rG'GnC_Cy
Cuts at: 2745 2745
Size: 5776
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2330 2330
Size: 5776
FspI TGC'GCA
Cuts at: 2857 4282 2857
Size: 1425 4351
HaeII r_GCGC'y
Cuts at: 1678 2039 2623 4923 1678
Size: 361 584 2300 2531
Fragments arranged by size:
2531 2300 584 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2555 2587 2625 2657 2696
Size: 32 135 32 983 32 38 32 39
Cuts at: 2696 2728 2834 2866 4417 4449 4491 4523 1373
Size: 32 106 32 1551 32 42 32 2626
Fragments arranged by size:
2626 1551 983 135 106 42 39 38
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3120 3366 669
Size: 988 281 1035 147 246 3079
Fragments arranged by size:
3079 1035 988 281 246 147
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2835 4890 5074 1305
Size: 1530 2055 184 2007
Fragments arranged by size:
2055 2007 1530 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2672 10
Size: 54 1425 343 840 3114
Fragments arranged by size:
3114 1425 840 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2095 2171 2730 3734 4093 4252 518
Size: 648 929 76 559 1004 359 159 2042
Fragments arranged by size:
2042 1004 929 648 559 359 159 76
MspA1I CmG'CkG
Cuts at: 1606 2215 2235 2915 2982 3192 3869 5017 5262
Size: 609 20 680 67 210 677 1148 245
Cuts at: 5262 1606
Size: 2120
Fragments arranged by size:
2120 1148 680 677 609 245 210 67
20
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4285
Fragments arranged by size:
4285 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 2916 3223 2916
Size: 307 5469
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PfoI T'CCnGG_A
Cuts at: 2127 2421 2127
Size: 294 5482
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2147 2179 2147
Size: 32 5744
PstI C_TGCA'G
Cuts at: 830 2545 3128 830
Size: 1715 583 3478
Fragments arranged by size:
3478 1715 583
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2215 2215
Size: 5776
SacII CC_GC'GG
Cuts at: 2983 2983
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SapI GCTCTTCn'nnn_
Cuts at: 2835 3000 2835
Size: 165 5611
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SfcI C'TryA_G
Cuts at: 826 1045 2541 3124 4259 4940 5131 826
Size: 219 1496 583 1135 681 191 1471
Fragments arranged by size:
1496 1471 1135 681 583 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 2208 3103 1872
Size: 336 895 4545
Fragments arranged by size:
4545 895 336
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2694 3880 4781 5043 820
Size: 197 926 751 1186 901 262 1553
Fragments arranged by size:
1553 1186 926 901 751 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 2199 1517
Size: 682 5094
StyI C'CwwG_G
Cuts at: 513 1065 2004 3215 513
Size: 552 939 1211 3074
Fragments arranged by size:
3074 1211 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2277 2700 2734 3803 3820 3973 4158
Size: 1255 697 423 34 1069 17 153 185
Cuts at: 4158 4603 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1069 697 445 423 185 153
34 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 2710 1316
Size: 1394 4382
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 2206 3101 1870
Size: 336 895 4545
Fragments arranged by size:
4545 895 336
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI XcmI