Restriction map for
pFN21AE0895
(Circular) (Six-base) MAPSORT of: pFN21AE0895.seq Check: 2421 from: 1 to: 5644
pFN21AE0895 5644 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5644
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3707 5091 5359 1632
Size: 2075 1384 268 1917
Fragments arranged by size:
2075 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 4543 5520 1203
Size: 3340 977 1327
Fragments arranged by size:
3340 1327 977
AgeI A'CCGG_T
Cuts at: 2367 4475 2367
Size: 2108 3536
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 2582 2807 1166
Size: 1416 225 4003
Fragments arranged by size:
4003 1416 225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 3387 4959 3387
Size: 1572 4072
ApaLI G'TGCA_C
Cuts at: 2569 3701 4857 2569
Size: 1132 1156 3356
Fragments arranged by size:
3356 1156 1132
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2441 2524 2965 4420 618
Size: 325 217 627 654 83 441 1455 1842
Fragments arranged by size:
1842 1455 654 627 441 325 217 83
BanII G_rGCy'C
Cuts at: 1391 2905 2963 1391
Size: 1514 58 4072
Fragments arranged by size:
4072 1514 58
BbeI G_GCGC'C
Cuts at: 2445 2445
Size: 5644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2550 2584 3835 3869 1903
Size: 34 613 34 1251 34 3678
Fragments arranged by size:
3678 1251 613 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 2450 4255
Size: 107 122 71 631 605 685 93 1805
Cuts at: 4255 136
Size: 1525
Fragments arranged by size:
1805 1525 685 631 605 122 107 93
71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2573 3705 4861 1790
Size: 783 1132 1156 2573
Fragments arranged by size:
2573 1156 1132 783
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4304 1239
Size: 3065 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2899 3769 4634 4932 2899
Size: 870 865 298 3611
Fragments arranged by size:
3611 870 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2883 4307 882
Size: 617 1384 1424 2219
Fragments arranged by size:
2219 1424 1384 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 2597 5638 2597
Size: 3041 2603
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2442 2775 3451 3833
Size: 53 83 186 936 909 333 676 382
Cuts at: 3833 4468 275
Size: 635 1451
Fragments arranged by size:
1451 936 909 676 635 382 333 186
83 53
BsaWI w'CCGG_w
Cuts at: 2367 4077 4475 4749 4896 5307 2367
Size: 1710 398 274 147 411 2704
Fragments arranged by size:
2704 1710 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2782 1186
Size: 1596 4048
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2573 3705 3790 4861 2573
Size: 1132 85 1071 3356
Fragments arranged by size:
3356 1132 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
BsmBI CGTCTCn'nnnn_
Cuts at: 2069 2504 2069
Size: 435 5209
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2573 2905 2963 3705 3790 4861 1391
Size: 399 783 332 58 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 399 332 85 58
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 2409 2679 3533 5263 2409
Size: 270 854 1730 2790
Fragments arranged by size:
2790 1730 854 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2732 2999 844
Size: 1295 593 267 3489
Fragments arranged by size:
3489 1295 593 267
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2367 4288 4475 1700
Size: 110 557 1921 187 2869
Fragments arranged by size:
2869 1921 557 187 110
BsrGI T'GTAC_A
Cuts at: 96 2835 96
Size: 2739 2905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstXI CCAn_nnnn'nTGG
Cuts at: 2324 2324
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2592 2974 3341 3726 3743 5195 5281 5293
Size: 620 382 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 620 385 382 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4153
Fragments arranged by size:
4153 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 3954 3974 3160
Size: 794 20 4830
Fragments arranged by size:
4830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2617 2652 529
Size: 35 2053 35 3521
Fragments arranged by size:
3521 2053 35 35
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2193 2223
Size: 54 1425 50 275 18 315 48 30
Cuts at: 2223 2376 2673 3091 3980 5609 8
Size: 153 297 418 889 1629 43
Fragments arranged by size:
1629 1425 889 418 315 297 275 153
54 50 48 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2759 3574 1353
Size: 1406 815 3423
Fragments arranged by size:
3423 1406 815
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3707 4304 5091 5359 1239
Size: 393 2075 597 787 268 1524
Fragments arranged by size:
2075 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2686 2686
Size: 5644
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 5644
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2445 4791 1678
Size: 767 2346 2531
Fragments arranged by size:
2531 2346 767
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2482 2514 4285 4317 4359
Size: 32 135 32 910 32 1771 32 42
Cuts at: 4359 4391 1373
Size: 32 2626
Fragments arranged by size:
2626 1771 910 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2920 2988 3234 669
Size: 988 281 982 68 246 3079
Fragments arranged by size:
3079 988 982 281 246 68
HindIII A'AGCT_T
Cuts at: 748 2820 748
Size: 2072 3572
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KasI G'GCGC_C
Cuts at: 2441 2441
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2584 4758 4942 1305
Size: 1279 2174 184 2007
Fragments arranged by size:
2174 2007 1279 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 2195 2225 2378 2675
Size: 54 1425 343 315 48 30 153 297
Cuts at: 2675 10
Size: 2979
Fragments arranged by size:
2979 1425 343 315 297 153 54 48
30
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2177 2582 2669 2807 2852 3602
Size: 648 954 57 405 87 138 45 750
Cuts at: 3602 3961 4120 518
Size: 359 159 2042
Fragments arranged by size:
2042 954 750 648 405 359 159 138
87 57 45
MspA1I CmG'CkG
Cuts at: 1606 3060 3737 4885 5130 1606
Size: 1454 677 1148 245 2120
Fragments arranged by size:
2120 1454 1148 677 245
NarI GG'CG_CC
Cuts at: 2442 2442
Size: 5644
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4153
Fragments arranged by size:
4153 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2107 3002 4547 1132
Size: 97 878 895 1545 2229
Fragments arranged by size:
2229 1545 895 878 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2686 2686
Size: 5644
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2698 2729 2996 830
Size: 1868 31 267 3478
Fragments arranged by size:
3478 1868 267 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5644
SfcI C'TryA_G
Cuts at: 826 1045 2694 2725 2872 2992 4127 4808 4999
Size: 219 1649 31 147 120 1135 681 191
Cuts at: 4999 826
Size: 1471
Fragments arranged by size:
1649 1471 1135 681 219 191 147 120
31
SfoI GGC'GCC
Cuts at: 2443 2443
Size: 5644
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2878 3748 4649 4911 820
Size: 197 926 935 870 901 262 1553
Fragments arranged by size:
1553 935 926 901 870 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2004 3083 513
Size: 552 939 1079 3074
Fragments arranged by size:
3074 1079 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2785 3671 3688 3841 4026 4471 325
Size: 1255 1205 886 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1205 886 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2307 2835
Size: 274 80 33 51 1443 204 126 528
Cuts at: 2835 3890 5602 96
Size: 1055 1712 138
Fragments arranged by size:
1712 1443 1055 528 274 204 138 126
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 2339 1316
Size: 1023 4621
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2203
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 2714 2864 3773 2714
Size: 150 909 4585
Fragments arranged by size:
4585 909 150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SrfI SwaI