Restriction map for
pFN21AE0893
(Circular) (Six-base) MAPSORT of: pFN21AE0893.seq Check: 2619 from: 1 to: 5746
pFN21AE0893 5746 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3809 5193 5461 1632
Size: 2177 1384 268 1917
Fragments arranged by size:
2177 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2024 820
Size: 197 1007 4542
Fragments arranged by size:
4542 1007 197
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 2954 1166
Size: 1788 3958
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 3489 5061 3489
Size: 1572 4174
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 2365 3036 3055 3281 3433 5542 1493
Size: 872 671 19 226 152 2109 1697
Fragments arranged by size:
2109 1697 872 671 226 152 19
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 3071 1870
Size: 73 1128 4545
Fragments arranged by size:
4545 1128 73
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3067 4522 618
Size: 325 217 627 1280 1455 1842
Fragments arranged by size:
1842 1455 1280 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3065 1391
Size: 1674 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4775
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 1903
Size: 34 2000 34 3678
Fragments arranged by size:
3678 2000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2468 4357 136
Size: 107 122 71 631 605 796 1889 1525
Fragments arranged by size:
1889 1525 796 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 3807 4963 1790
Size: 2017 1156 2573
Fragments arranged by size:
2573 2017 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2680 4406 1239
Size: 1441 1726 2579
Fragments arranged by size:
2579 1726 1441
Bpu10I CC'TnA_GC
Cuts at: 2178 2725 2178
Size: 547 5199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 4736 5034 3871
Size: 865 298 4583
Fragments arranged by size:
4583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2714 4409 882
Size: 617 1215 1695 2219
Fragments arranged by size:
2219 1695 1215 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3553 3935 4570 275
Size: 53 83 186 936 2020 382 635 1451
Fragments arranged by size:
2020 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2067 2097 2552 2582 2678
Size: 30 165 30 664 30 455 30 96
Cuts at: 2678 2708 1178
Size: 30 4216
Fragments arranged by size:
4216 664 455 165 96 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5746
BseYI C'CCAG_C
Cuts at: 2658 4949 5445 2658
Size: 2291 496 2959
Fragments arranged by size:
2959 2291 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5173
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2479 3196 3957 4985 1540
Size: 275 186 478 717 761 1028 2301
Fragments arranged by size:
2301 1028 761 717 478 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 2793 3807 3892 4963 2247
Size: 546 1014 85 1071 3030
Fragments arranged by size:
3030 1071 1014 546 85
BsmI GAATG_Cn'
Cuts at: 3257 3350 3257
Size: 93 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2793 3065 3807 3892 4963 1391
Size: 399 457 546 272 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 546 457 399 272 85
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 2463 2778 3101 844
Size: 1385 234 315 323 3489
Fragments arranged by size:
3489 1385 323 315 234
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2533 4241 4415 65
Size: 1183 1285 1708 174 1396
Fragments arranged by size:
1708 1396 1285 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2478 4390 4577 1700
Size: 110 668 1912 187 2869
Fragments arranged by size:
2869 1912 668 187 110
BsrGI T'GTAC_A
Cuts at: 96 2946 96
Size: 2850 2896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2539 794
Size: 1745 4001
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2704 2113
Size: 591 5155
BstYI r'GATC_y
Cuts at: 1972 3076 3443 3828 3845 5297 5383 5395 5741
Size: 1104 367 385 17 1452 86 12 346
Cuts at: 5741 1972
Size: 1977
Fragments arranged by size:
1977 1452 1104 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2143 2990 2143
Size: 847 4899
BtgI C'CryG_G
Cuts at: 513 1065 2528 513
Size: 552 1463 3731
Fragments arranged by size:
3731 1463 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 2075 2850 3262 4056 4076 2075
Size: 775 412 794 20 3745
Fragments arranged by size:
3745 794 775 412 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2469 2487
Size: 54 1425 50 275 18 426 213 18
Cuts at: 2487 3193 4082 5711 8
Size: 706 889 1629 43
Fragments arranged by size:
1629 1425 889 706 426 275 213 54
50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2173 3002 3676 1353
Size: 820 829 674 3423
Fragments arranged by size:
3423 829 820 674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2680 3809 4406 5193 5461 1239
Size: 393 1048 1129 597 787 268 1524
Fragments arranged by size:
1524 1129 1048 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2898 2898
Size: 5746
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
EcoRV GAT'ATC
Cuts at: 2326 2326
Size: 5746
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 4893 1678
Size: 3215 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2678 2710 4387 4419 4461
Size: 32 135 32 1106 32 1677 32 42
Cuts at: 4461 4493 1373
Size: 32 2626
Fragments arranged by size:
2626 1677 1106 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2027 748
Size: 1279 4467
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2196 4860 5044 1305
Size: 891 2664 184 2007
Fragments arranged by size:
2664 2007 891 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2471 2489 10
Size: 54 1425 343 639 18 3267
Fragments arranged by size:
3267 1425 639 343 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 2511 2942 2954 3704 4063 4222
Size: 648 1122 223 431 12 750 359 159
Cuts at: 4222 518
Size: 2042
Fragments arranged by size:
2042 1122 750 648 431 359 223 159
12
MspA1I CmG'CkG
Cuts at: 1606 3162 3839 4987 5232 1606
Size: 1556 677 1148 245 2120
Fragments arranged by size:
2120 1556 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5194
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 3104 4649 1132
Size: 97 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5746
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2707 2817 1108
Size: 1599 110 4037
Fragments arranged by size:
4037 1599 110
PfoI T'CCnGG_A
Cuts at: 2096 2096
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PstI C_TGCA'G
Cuts at: 830 2187 2809 2857 3098 830
Size: 1357 622 48 241 3478
Fragments arranged by size:
3478 1357 622 241 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 2128 2183 2805 2853 2983 3094 4229
Size: 219 1083 55 622 48 130 111 1135
Cuts at: 4229 4910 5101 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1083 681 622 219 191 130
111 55 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 2468 2468
Size: 5746
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2024 3850 4751 5013 820
Size: 197 926 81 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 262 197 81
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 2825 1517
Size: 1308 4438
StyI C'CwwG_G
Cuts at: 513 1065 3185 513
Size: 552 2120 3074
Fragments arranged by size:
3074 2120 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2672 2977 3773 3790 3943 4128 4573
Size: 1255 1092 305 796 17 153 185 445
Cuts at: 4573 325
Size: 1498
Fragments arranged by size:
1498 1255 1092 796 445 305 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2292 2418 2910
Size: 274 80 33 51 1443 315 126 492
Cuts at: 2910 2946 3992 5704 96
Size: 36 1046 1712 138
Fragments arranged by size:
1712 1443 1046 492 315 274 138 126
80 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 2564 2596 5706 5738 2067
Size: 32 465 32 3110 32 2075
Fragments arranged by size:
3110 2075 465 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2479 2704 2914 2479
Size: 225 210 5311
Fragments arranged by size:
5311 225 210
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 2825 2975 3875 2825
Size: 150 900 4696
Fragments arranged by size:
4696 900 150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfoI
SrfI SwaI